Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 4,653,487 A→T 6.3% N251I (AAC→ATC)  nuoG → NADH‑quinone oxidoreductase subunit G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,653,4870AT 6.3% 210.0 / 4.6 63N251I (AAC→ATC) nuoGNADH‑quinone oxidoreductase subunit G
Reads supporting (aligned to +/- strand):  ref base A (27/32);  new base T (2/2);  total (29/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.56e-01

CAAGACCCACTCCGAACGCTACAACCGCAAGTGGGACATGCAGTTCGCCCCAAGCATCTGCCATGGCTGCTCCAGCGGCTGCAACATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGCCGCTTCGGCTACGGCTACGTCAACCGCAAGGACCGCCCACGCCAGCCACAACTGGCCGACGGCACCAAGCTGGGCCTGGACGCC  >  ACB143/4653359‑4653617
                                                                                                                                |                                                                                                                                  
cAAGACCCACTCCGAACGCTACAACCGCAAGTGGGACATGCAGTTCGCCCCAAGCATCTGCCATGGCTGCTCCAGCGGCTGCAACATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCtt                                                                                                                              >  4:239344/1‑135 (MQ=255)
cAAGACCCACTCCGAACGCTACAACCGCAAGTGGGACATGCAGTTCGCCCCAAGCATCTGCCATGGCTGCTCCAGCGGCTGCAACATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCtt                                                                                                                              >  2:300606/1‑135 (MQ=255)
       cACTCCGAACGCTACAACCGCAAGTGGGACATGCAGTTCGCCCCAAGCATCTGCCATGGCTGCTCCAGCGGCTGCAACATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGtt                                                                                                                      <  3:142204/136‑1 (MQ=255)
         cTCCGAACGCTACAACCGCAAGTGGGACATGCAGTTCGCCCGAAGCATCTGCCATGGCTGCTCCAGCGGCTGCAACATCAGCCCTGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACAGCTTCAACGGTTCg                                                                                                                    <  4:105260/136‑1 (MQ=255)
            cGAACGCTACAACCGCAAGTGGGACATGCAGTTCGCCCCAAGCATCTGCCATGGCTGCTCCAGCGGCTGCAACATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCgg                                                                                                                   <  8:2070/134‑1 (MQ=255)
            cGAACGCTACAACCGCAAGTGGGACATGCAGTTCGCCCCAAGCATCTGCCATGGCTGCTCCAGCGGCTGCAACATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGt                                                                                                                  <  5:324487/135‑1 (MQ=255)
            cGAACGCTACAACCGCAAGTGGGACATGCAGTTCGCCCCAAACATCTGCCATGGCTGCTCCAGCGGCTGCAACATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGt                                                                                                                  <  5:143916/135‑1 (MQ=255)
             gAACGCTACAACCGCAAGTGGGACATGCAGTTCGCCCCAAGCATCTGCCATGGCTGCTCCAGCGGCTGCAACATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTc                                                                                                                 >  1:276827/1‑135 (MQ=255)
             gAACGCTACAACCGCAAGTGGGACATGCAGTTCGCCCCAAGCATCTGCCATGGCTGCTCCAGCGGCTGCAACATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCa                                                                                                                <  6:49591/136‑1 (MQ=255)
             gAACGCTACAACCGCAAGTGGGACATGCAGTTCGCCCCAAGCATCTGCCATGGCTGCTCCAGCGGCTGCAACATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCa                                                                                                                <  3:76775/136‑1 (MQ=255)
             gAACGCTACAACCGCAAGTGGGACATGCAGTTCGCCCCAAGCATCTGCCATGGCTGCTCCAGCGGCTGCAACATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCa                                                                                                                <  7:354753/136‑1 (MQ=255)
               aCGCTACAACCGCAAGTGGGACATGCAGTTCGCCCCAAGCATCTGCCATGGCTGCTCCAGCGGCTGCAACATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAAc                                                                                                              <  1:66189/136‑1 (MQ=255)
                cGCTACAACCGCAAGTGGGACATGCAGTTCGCCCCAAGCATCTGCCATGGCTGCTCCAGCGGCTGCAACATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAAcc                                                                                                             <  7:78117/136‑1 (MQ=255)
                 gCTACAACCGCAAGTGGGACATGCAGTTCGCCCCAAGCATCTGCCATGGCTGCTCCAGCGGCTGCAACATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGGTCGGTCAAc                                                                                                              >  7:31194/1‑134 (MQ=255)
                       aCCGCAAGTGGGACATGTAGTTCGCCCCAAGCATCTGCCATGGCTGCTCCAGCGGCTGCAACATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACAGCTTCAACGGTTCGGTCAACCAGTACt                                                                                                       >  7:115380/1‑135 (MQ=255)
                              gTGGGACATGCAGTTCGCCCCAAGCATCTGCCATGGCTGCTCCAGCGGCTGCAACATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGc                                                                                               >  7:202341/1‑136 (MQ=255)
                              gTGGGACATGCAGTTCGCCCCAAGCATCTGCCATGGCTGCTCCAGCGGCTGCAACATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGc                                                                                               >  2:113101/1‑136 (MQ=255)
                                  gACATGCAGTTCGCCCCAAGCATCTGCCATGGCTGCTCCAGCGGCTGCAACATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGAcc                                                                                           <  7:163236/136‑1 (MQ=255)
                                  gACATGCAGTTCGCCCCAAGCATCTGCCATGGCTGCTCCAGCGGCTGCAACATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGAcc                                                                                           <  6:327814/136‑1 (MQ=255)
                                     aTGCAGTTCGCCCCAAGCATCTGCCATGGCTGCTCCAGCGGCTGCAACCTCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACcgcg                                                                                        >  7:118680/1‑136 (MQ=255)
                                      tGCAGTTCGCCCCAAGCATCTGCCATGGCTGCTCCAGCGGCTGCAACATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCgg                                                                                       >  2:53841/1‑136 (MQ=255)
                                        cAGTTCGCCCCAAGCATCTGCCATGGCTGCTCCAGCGGCTGCAACATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGcc                                                                                     <  7:171114/136‑1 (MQ=255)
                                        cAGTTCGCCCCAAGCATCTGCCATGGCTGCTCCAGCGGCTGCAACATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGcc                                                                                     <  5:181052/136‑1 (MQ=255)
                                           ttCGCCCCAAGCATCTGCCATGGCTGCTCCAGCGGCTGCAACATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGCCGCt                                                                                  <  8:39703/136‑1 (MQ=255)
                                              gCCCCAAGCATCTGCCATGGCTGCTCCAGCGGCTGCAACATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGCCGCTTc                                                                                <  5:359992/135‑1 (MQ=255)
                                              gCCCCAAGCATCTGCCATGGCTGCTCCAGCGGCTGCAACATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGCCGCTTc                                                                                <  1:300606/135‑1 (MQ=255)
                                               ccccAAGCATCTGCCATGGCTGCTCCAGCGGCTGCAACATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGCCGCTTcgg                                                                              >  2:292443/1‑136 (MQ=255)
                                               ccccAAGCATCTGCCATGGCTGCGCCAGCGGCTGCAACATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGCCGCTTcgg                                                                              <  4:9942/136‑1 (MQ=255)
                                                cccAAGCATCTGCCATGGCTGCTCCAGCGGCTGCAACATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGCCGCTTcggc                                                                             >  1:23184/1‑136 (MQ=255)
                                                   aaGCATCTGCCATGGCTGCTCCAGCGGCTGCAACATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGCCGCTTcggctac                                                                          <  3:302138/136‑1 (MQ=255)
                                                            ccATGGCTGCTCCAGCGGCTGCAACATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGCCGCTTCGgctacggctacg                                                                   <  7:138338/134‑1 (MQ=255)
                                                            ccATGGCTGCTCCAGCGGCTGCAACATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGCCGCTTCGgctacggctacg                                                                   <  3:265887/134‑1 (MQ=255)
                                                            ccATGGCTGCTCCAGCGGCTGCAACATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGCCGCTTCGgctacggctacg                                                                   <  8:182567/134‑1 (MQ=255)
                                                            ccATGGCTGCTCCAGCGGCTGCAACATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGCCGCTTCGgctacggctacg                                                                   <  2:184196/134‑1 (MQ=255)
                                                            ccATGGCTGCTCCAGCGGCTGCAACATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGCCGCTTCGGCTACGGCTACGt                                                                  >  7:75057/1‑135 (MQ=255)
                                                             cATGGCTGCTCCAGCGGCTGCAACATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGCCGCTTCGGCTACGGCTACGTc                                                                 >  7:352909/1‑135 (MQ=255)
                                                             cATGGCTGCTCCAGCGGCTGCAACATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGCCGCGTCGGCTACGGCTACGTc                                                                 >  7:196198/1‑135 (MQ=255)
                                                                   tgctCCAGCGGCTGCAACATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGCCGCTTCGGCTACGGCTACGTCAACCGc                                                           >  5:194982/1‑135 (MQ=255)
                                                                        cAGCGGCTGCAACATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGCCGCTTCGGCTACGGCTACGTCAACCGCAAGGAc                                                     >  6:324846/1‑136 (MQ=255)
                                                                             gCTGCAACATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGCCGCTTCGGCTACGGCTACGTCAACCGCAAGGACCGccc                                                <  1:272124/136‑1 (MQ=255)
                                                                                   aCATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACAGCGGCCGCTTCGGCTACGGCTACGTCAACCGCAAGGACCGCCCACgcca                                          >  4:230358/1‑136 (MQ=255)
                                                                                                 gAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGCCGCTTCGGCTACGGCTACGTCAACCGCAAGGACCGCCCACGCCAGCCACAACTGGcc                             >  3:41122/1‑135 (MQ=255)
                                                                                                    cgcTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGCCGCTTCGGCTACGGCTACGTCAACCGCAAGGACCGCCCACACCAGCCACAACTGGCCGACg                         <  4:160688/136‑1 (MQ=255)
                                                                                                          ggCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGCCGCTTCGgctacggctacg                                                                   <  8:35829/88‑1 (MQ=255)
                                                                                                          ggCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGCCGCTTCGgctacggctacg                                                                   >  7:35829/1‑88 (MQ=255)
                                                                                                          ggCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGCCGCTTCGGCTACGGCTACGTCAACCGCAAGGACCGCCCACGCCAGCCACAACTGGCCGACGGCACCa                   <  4:31541/136‑1 (MQ=255)
                                                                                                          ggCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGCCGCTTCGGCTACGGCTACGTCAACCGCAAGGACCGCCCACGCCAGCCACAACTGGCCGACGGCACCa                   <  4:41122/136‑1 (MQ=255)
                                                                                                          ggCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGCCGCTTCGGCTACGGCTACGTCAACCGCAAGGACCGCCCACGCCAGCCACAACTGGCCGACGGCACCa                   <  3:229345/136‑1 (MQ=255)
                                                                                                            cGAACTACGCCGTATCGAAATCCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGCCGCTTCGGCTACGGCTACGTCAACCGCAAGGACCGCCCACgcc                                           <  1:154014/110‑1 (MQ=255)
                                                                                                            cGAACTACGCCGTATCGAAATCCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGCCGCTTCGGCTACGGCTACGTCAACCGCAAGGACCGCCCACgcc                                           <  1:148393/110‑1 (MQ=255)
                                                                                                            cGAACTACGCCGTATCGAAATCCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGCCGCTTCGGCTACGGCTACGTCAACCGCAAGGACCGCCCACgcc                                           >  2:148393/1‑110 (MQ=255)
                                                                                                            cGAACTACGCCGTATCGAAATCCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGCCGCTTCGGCTACGGCTACGTCAACCGCAAGGACCGCCCACgcc                                           >  2:154014/1‑110 (MQ=255)
                                                                                                                 tACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGCCGCTTCGGCTACGGCTACGTCAACCGCAAGGACCGCCCACGCCCGCCACAACTGGCCGACGGCACCAAGCTggg            >  8:185687/1‑136 (MQ=255)
                                                                                                                 tACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGCCGCTTCGGCTACGGCTACGTCAACCGCAAGGACCGCCCACGCCAGCCACAACTGGCCGACGGCACCAAGCTggg            >  3:230909/1‑136 (MQ=255)
                                                                                                                 tACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGCCGCTTCGGCTACGGCTACGTCAACCGCAAGGACCGCCCACGCCAGCCACAACTGGCCGACGGCACCAAGCTggg            >  1:274628/1‑136 (MQ=255)
                                                                                                                 tACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGCCGCTTCGGCTACGGCTACGTCAACCGCAAGGACCGCCCACGCCAGCCACAACTGGCCGACGGCACCAAGCTggg            >  1:57333/1‑136 (MQ=255)
                                                                                                                 tACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGCCGCTTCGGCTACGGCTACGTCAACCGCAAGGACCGCCCACGCCAGCCACAACTGGCCGACGGCACCAAGCTggg            >  4:150990/1‑136 (MQ=255)
                                                                                                                      cGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGCCGCTTCGGCTACGGCTACGTCAACCGCAAGGACCGCCCACGCCAGCCACAACTGGCCGACGGCACCAAGCTGGGCCTgg       <  8:167832/136‑1 (MQ=255)
                                                                                                                      agTATCGAAAACAGCTTCAACGGTTCGGTCACCCAGTACTTCCTGTGCGACCGCGGCCGCTTGGGCTTCGGCTACGTCAACCGCAAGGACCGCGCACGCCCGCGACGAATGGCCGAGGGGCGCTAGATGGGCCTgg       >  3:60413/2‑136 (MQ=255)
                                                                                                                           cGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGCCGCTTCGGCTACGGCTACGTCAACCGCAAGGACCGCCCACGCCAGCCACAACTGGCCGACGGCACCAAGCTGGGCCTGGAcgcc  >  3:384479/1‑136 (MQ=255)
                                                                                                                           cGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGCCGCTTCGGCTACGGCGACGTCAACCGCAAGGACCGCCCCCGCCAGCCACAACTGGCCGACGGCACCACGCTGGGCCTGGAcgcc  >  3:70714/1‑136 (MQ=255)
                                                                                                                            gAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGCCGCTTCGGCTACGGCTACGTCAACCGCAAGGACCGCCCACGCCAGCCACAACTGGCCGACGGCACCAAGCTGGGCCTGGAcgcc  <  6:211278/135‑1 (MQ=255)
                                                                                                                            gAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGCCGCTTCGGCTACGGCTACGTCAACCGCAAGGACCGCCCACGCCAGCCACAACTGGCCGACGGCACCAAGCTGGGCCTGGAcgcc  <  5:211533/135‑1 (MQ=255)
                                                                                                                                |                                                                                                                                  
CAAGACCCACTCCGAACGCTACAACCGCAAGTGGGACATGCAGTTCGCCCCAAGCATCTGCCATGGCTGCTCCAGCGGCTGCAACATCAGCCCGGGTGAGCGCTACGGCGAACTACGCCGTATCGAAAACCGCTTCAACGGTTCGGTCAACCAGTACTTCCTGTGCGACCGCGGCCGCTTCGGCTACGGCTACGTCAACCGCAAGGACCGCCCACGCCAGCCACAACTGGCCGACGGCACCAAGCTGGGCCTGGACGCC  >  ACB143/4653359‑4653617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑