Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 4,749,727 C→T 100% S441S (AGC→AGT ttgI_4 → Toluene efflux pump outer membrane protein TtgI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,749,7270CT100.0% 213.6 / ‑6.8 66S441S (AGC→AGTttgI_4Toluene efflux pump outer membrane protein TtgI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (31/33);  minor base G (1/1);  total (32/34)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTGACCGTGCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTG  >  ACB143/4749599‑4749850
                                                                                                                                |                                                                                                                           
cTGACCGTGCTCGACACCCAGCGCAGCCTGTACCCGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGAcgc                                                                                                                      <  5:353773/136‑1 (MQ=255)
   aCCGTGCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGAcgcg                                                                                                                     <  4:120162/134‑1 (MQ=255)
   aCCGTGCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGAcgcg                                                                                                                     <  2:334865/134‑1 (MQ=255)
   aCCGTGCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGAcgcg                                                                                                                     <  7:324236/134‑1 (MQ=255)
        gCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGc                                                                                                              <  3:354044/136‑1 (MQ=255)
        gCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCGAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAACGCCTTCGGCGGTGGCTGGCAAGCGGATAGGCCGACGCGCTAGGGc                                                                                                              >  7:339176/1‑136 (MQ=255)
         cTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGc                                                                                                              <  7:265399/135‑1 (MQ=255)
         cTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGc                                                                                                              <  6:138174/135‑1 (MQ=255)
         cTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGc                                                                                                              <  5:160705/135‑1 (MQ=255)
         cTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCCAGGGc                                                                                                              <  1:280649/135‑1 (MQ=255)
           cGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTACGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGt                                                                                                            >  8:242180/1‑135 (MQ=255)
            gACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGt                                                                                                            <  4:32732/134‑1 (MQ=255)
            gACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTc                                                                                                           <  1:175994/135‑1 (MQ=255)
             acacCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAg                                                                                                         <  4:402554/136‑1 (MQ=255)
                cccAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGc                                                                                                       >  4:397459/1‑135 (MQ=255)
                  cAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTAt                                                                                                    <  7:71897/136‑1 (MQ=255)
                  cAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTAt                                                                                                    <  7:343006/136‑1 (MQ=255)
                  cAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTAt                                                                                                    >  4:323016/1‑136 (MQ=255)
                     cgcAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTg                                                                                                 <  3:332168/136‑1 (MQ=255)
                     cgcAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTg                                                                                                 <  1:243711/136‑1 (MQ=255)
                      gcAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTgg                                                                                                >  7:84306/1‑136 (MQ=255)
                       cAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGgt                                                                                               <  6:163567/136‑1 (MQ=255)
                                 cAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGAcc                                                                                     >  7:164122/1‑136 (MQ=255)
                                    gCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGTCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCt                                                                                  <  1:354350/136‑1 (MQ=255)
                                    gCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCt                                                                                  <  7:9729/136‑1 (MQ=255)
                                    gCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCt                                                                                  <  5:74545/136‑1 (MQ=255)
                                       cAGGACCAGCAGTCCCGAAAGAACCAAACAAAACTGCAGGCCAGCGTGGCCCTTTACCAAGCCTTGGGCGGTGGCTGCCAAGCGGATAGTCCGAAGCGCTAGGTCGTCAGGCTATCTGGTGTACACGACCGCTGcc                                                                               >  3:384887/1‑136 (MQ=255)
                                       cAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGCCGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGcc                                                                               >  7:81465/1‑136 (MQ=255)
                                       cAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGcc                                                                               >  3:298157/1‑136 (MQ=255)
                                       cAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGcc                                                                               >  8:39138/1‑136 (MQ=255)
                                       cAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGcc                                                                               >  5:7840/1‑136 (MQ=255)
                                       cAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCGTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCCCGACCGCTGcc                                                                               >  3:303479/1‑136 (MQ=255)
                                           aCCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGAc                                                                           >  2:274192/1‑136 (MQ=255)
                                                  ggCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAgg                                                                    >  7:55979/1‑136 (MQ=255)
                                                  ggCCCGCCTGCAGCTAAGCAAGCTTCAGGCCAGCGTGGCCCTTTACAACGCCATGGGGGCTGGCTGGCAAGCGGATAGTCAGACGCGCTAGTGCCTCAGGCTATCTGGTGTTCCCGACCGCTGCGTGACCTTCAgc                                                                    >  2:382815/1‑135 (MQ=255)
                                                   gCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGt                                                                   <  3:77287/136‑1 (MQ=255)
                                                   gCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGt                                                                   <  6:332499/136‑1 (MQ=255)
                                                   gCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGt                                                                   <  6:109314/136‑1 (MQ=255)
                                                      cGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGTATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGc                                                                <  1:102029/136‑1 (MQ=255)
                                                                 aaGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGGCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAg                                                     <  1:303186/136‑1 (MQ=255)
                                                                 aaGCCAACTGCAGGCCAGCGGGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAg                                                     <  5:203247/136‑1 (MQ=255)
                                                                      aaCTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGggcg                                                <  5:851/136‑1 (MQ=255)
                                                                                 aGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGTGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACtt                                     >  1:244623/1‑136 (MQ=255)
                                                                                   cGTGGCCCTTTACAAATCCTTGGGCGGTGGCTTGCAAGCGGAGAGTCCGACGCGGTAGGGCGTCAGGCTATCTGGTTTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTg                                   <  4:238853/136‑1 (MQ=255)
                                                                                    gTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGCCCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGc                                  >  5:295164/1‑136 (MQ=255)
                                                                                      ggCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGcc                                 >  5:297978/1‑135 (MQ=255)
                                                                                      ggCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGcc                                 >  7:100921/1‑135 (MQ=255)
                                                                                      ggCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGcc                                 >  6:192515/1‑135 (MQ=255)
                                                                                      ggCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGcc                                 >  2:187757/1‑135 (MQ=255)
                                                                                           tttACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAgg                                                                                                        <  2:335494/59‑1 (MQ=255)
                                                                                           tttACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAgg                                                                                                        >  1:335494/1‑59 (MQ=255)
                                                                                           tttACAAAGCCTTGGGCGGTGGCTGGAAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAg                           >  5:233423/1‑136 (MQ=255)
                                                                                               cAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCg                       >  4:49796/1‑136 (MQ=255)
                                                                                                  aGCCGTGGGCGGGGGCGGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCa                    <  8:210440/136‑1 (MQ=255)
                                                                                                       tGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAgcg               >  4:70859/1‑136 (MQ=255)
                                                                                                       tGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAgcg               >  8:339981/1‑136 (MQ=255)
                                                                                                          gCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGCCCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGctc            >  1:104179/1‑136 (MQ=255)
                                                                                                          gCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGctc            >  7:81507/1‑136 (MQ=255)
                                                                                                            ggTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGctcct          <  3:55459/136‑1 (MQ=255)
                                                                                                            ggTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGctcct          <  3:11118/136‑1 (MQ=255)
                                                                                                                 ctggcAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTAt                                                                                                    >  2:237842/1‑41 (MQ=255)
                                                                                                                 ctggcAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTAt                                                                                                    <  1:237842/41‑1 (MQ=255)
                                                                                                                 ctggcAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCtcgtcg     >  6:18930/1‑136 (MQ=255)
                                                                                                                 ctggcAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCtcgtcg     >  3:389752/1‑136 (MQ=255)
                                                                                                                 ctggcAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCgcgtcg     >  3:302379/1‑136 (MQ=255)
                                                                                                                    gcAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTg  <  6:233423/136‑1 (MQ=255)
                                                                                                                                |                                                                                                                           
CTGACCGTGCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTG  >  ACB143/4749599‑4749850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: