Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | ACB143 | 4,749,727 | C→T | 100% | S441S (AGC→AGT) | ttgI_4 → | Toluene efflux pump outer membrane protein TtgI |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | ACB143 | 4,749,727 | 0 | C | T | 100.0% | 213.6 / ‑6.8 | 66 | S441S (AGC→AGT) | ttgI_4 | Toluene efflux pump outer membrane protein TtgI |
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (31/33); minor base G (1/1); total (32/34) | |||||||||||
Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
CTGACCGTGCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTG > ACB143/4749599‑4749850 | cTGACCGTGCTCGACACCCAGCGCAGCCTGTACCCGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGAcgc < 5:353773/136‑1 (MQ=255) aCCGTGCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGAcgcg < 4:120162/134‑1 (MQ=255) aCCGTGCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGAcgcg < 2:334865/134‑1 (MQ=255) aCCGTGCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGAcgcg < 7:324236/134‑1 (MQ=255) gCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGc < 3:354044/136‑1 (MQ=255) gCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCGAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAACGCCTTCGGCGGTGGCTGGCAAGCGGATAGGCCGACGCGCTAGGGc > 7:339176/1‑136 (MQ=255) cTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGc < 7:265399/135‑1 (MQ=255) cTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGc < 6:138174/135‑1 (MQ=255) cTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGc < 5:160705/135‑1 (MQ=255) cTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCCAGGGc < 1:280649/135‑1 (MQ=255) cGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTACGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGt > 8:242180/1‑135 (MQ=255) gACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGt < 4:32732/134‑1 (MQ=255) gACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTc < 1:175994/135‑1 (MQ=255) acacCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAg < 4:402554/136‑1 (MQ=255) cccAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGc > 4:397459/1‑135 (MQ=255) cAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTAt < 7:71897/136‑1 (MQ=255) cAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTAt < 7:343006/136‑1 (MQ=255) cAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTAt > 4:323016/1‑136 (MQ=255) cgcAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTg < 3:332168/136‑1 (MQ=255) cgcAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTg < 1:243711/136‑1 (MQ=255) gcAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTgg > 7:84306/1‑136 (MQ=255) cAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGgt < 6:163567/136‑1 (MQ=255) cAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGAcc > 7:164122/1‑136 (MQ=255) gCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGTCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCt < 1:354350/136‑1 (MQ=255) gCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCt < 7:9729/136‑1 (MQ=255) gCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCt < 5:74545/136‑1 (MQ=255) cAGGACCAGCAGTCCCGAAAGAACCAAACAAAACTGCAGGCCAGCGTGGCCCTTTACCAAGCCTTGGGCGGTGGCTGCCAAGCGGATAGTCCGAAGCGCTAGGTCGTCAGGCTATCTGGTGTACACGACCGCTGcc > 3:384887/1‑136 (MQ=255) cAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGCCGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGcc > 7:81465/1‑136 (MQ=255) cAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGcc > 3:298157/1‑136 (MQ=255) cAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGcc > 8:39138/1‑136 (MQ=255) cAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGcc > 5:7840/1‑136 (MQ=255) cAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCGTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCCCGACCGCTGcc > 3:303479/1‑136 (MQ=255) aCCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGAc > 2:274192/1‑136 (MQ=255) ggCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAgg > 7:55979/1‑136 (MQ=255) ggCCCGCCTGCAGCTAAGCAAGCTTCAGGCCAGCGTGGCCCTTTACAACGCCATGGGGGCTGGCTGGCAAGCGGATAGTCAGACGCGCTAGTGCCTCAGGCTATCTGGTGTTCCCGACCGCTGCGTGACCTTCAgc > 2:382815/1‑135 (MQ=255) gCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGt < 3:77287/136‑1 (MQ=255) gCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGt < 6:332499/136‑1 (MQ=255) gCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGt < 6:109314/136‑1 (MQ=255) cGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGTATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGc < 1:102029/136‑1 (MQ=255) aaGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGGCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAg < 1:303186/136‑1 (MQ=255) aaGCCAACTGCAGGCCAGCGGGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAg < 5:203247/136‑1 (MQ=255) aaCTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGggcg < 5:851/136‑1 (MQ=255) aGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGTGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACtt > 1:244623/1‑136 (MQ=255) cGTGGCCCTTTACAAATCCTTGGGCGGTGGCTTGCAAGCGGAGAGTCCGACGCGGTAGGGCGTCAGGCTATCTGGTTTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTg < 4:238853/136‑1 (MQ=255) gTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGCCCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGc > 5:295164/1‑136 (MQ=255) ggCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGcc > 5:297978/1‑135 (MQ=255) ggCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGcc > 7:100921/1‑135 (MQ=255) ggCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGcc > 6:192515/1‑135 (MQ=255) ggCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGcc > 2:187757/1‑135 (MQ=255) tttACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAgg < 2:335494/59‑1 (MQ=255) tttACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAgg > 1:335494/1‑59 (MQ=255) tttACAAAGCCTTGGGCGGTGGCTGGAAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAg > 5:233423/1‑136 (MQ=255) cAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCg > 4:49796/1‑136 (MQ=255) aGCCGTGGGCGGGGGCGGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCa < 8:210440/136‑1 (MQ=255) tGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAgcg > 4:70859/1‑136 (MQ=255) tGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAgcg > 8:339981/1‑136 (MQ=255) gCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGCCCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGctc > 1:104179/1‑136 (MQ=255) gCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGctc > 7:81507/1‑136 (MQ=255) ggTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGctcct < 3:55459/136‑1 (MQ=255) ggTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGctcct < 3:11118/136‑1 (MQ=255) ctggcAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTAt > 2:237842/1‑41 (MQ=255) ctggcAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTAt < 1:237842/41‑1 (MQ=255) ctggcAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCtcgtcg > 6:18930/1‑136 (MQ=255) ctggcAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCtcgtcg > 3:389752/1‑136 (MQ=255) ctggcAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCgcgtcg > 3:302379/1‑136 (MQ=255) gcAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTg < 6:233423/136‑1 (MQ=255) | CTGACCGTGCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTG > ACB143/4749599‑4749850 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |