Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 4,759,509 G→A 100% intergenic (+124/‑16) fpvA_3 → / → ligM_KToptimized Ferripyoverdine receptor/sequence generated by OPTIMIZER for KT2440

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,759,5090GA100.0% 152.0 / ‑6.8 45intergenic (+124/‑16)fpvA_3/ligM_KToptimizedFerripyoverdine receptor/sequence generated by OPTIMIZER for KT2440
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (24/20);  minor base T (0/1);  total (24/21)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCC  >  ACB143/4759377‑4759631
                                                                                                                                    |                                                                                                                          
aGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATGTCACACCAACACATAGAGtt                                                                                                                         >  2:96947/1‑136 (MQ=255)
 gCTTCTGATCTCCTGACGTAGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGttt                                                                                                                        <  2:122541/136‑1 (MQ=255)
 gCTTCTGATCGCCTGACGTTGAACGCAAAAATCCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACATTTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGttt                                                                                                                        <  5:19276/136‑1 (MQ=255)
 gCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGttt                                                                                                                        <  7:124059/136‑1 (MQ=255)
 gCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGttt                                                                                                                        <  4:393018/136‑1 (MQ=255)
 gCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGttt                                                                                                                        <  4:78885/136‑1 (MQ=255)
 gCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGttt                                                                                                                        <  6:299441/136‑1 (MQ=255)
 gCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGttt                                                                                                                        <  7:191862/136‑1 (MQ=255)
     ttGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTag                                                                                                                      <  4:34132/133‑1 (MQ=255)
     cTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTag                                                                                                                      >  3:166253/1‑134 (MQ=255)
     cTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTag                                                                                                                      >  6:268915/1‑134 (MQ=255)
     cTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAAAACATAGAGTTTag                                                                                                                      >  8:7894/1‑134 (MQ=255)
          cGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGta                                                                                                                >  6:347246/1‑135 (MQ=255)
                aCGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGa                                                                                                         <  8:188594/136‑1 (MQ=255)
                         gCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGa                                                                                                >  7:88020/1‑136 (MQ=255)
                                       ccAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAg                                                                                  >  3:236255/1‑136 (MQ=255)
                                                gTTTTTTGAGCTGTTGACAATTAATCATCGGCGCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGgcc                                                                         <  6:100222/136‑1 (MQ=255)
                                                        aGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCcggcgg                                                                 >  2:42704/1‑136 (MQ=255)
                                                             ttGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAaca                                                            >  6:190290/1‑136 (MQ=255)
                                                                aCAATTAATCATCGGCTCTTATAATGTGTGTAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCg                                                         <  8:312631/136‑1 (MQ=255)
                                                                aCAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCg                                                         >  6:170388/1‑136 (MQ=255)
                                                                       atcatcGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAAt                                                  <  7:56955/136‑1 (MQ=255)
                                                                          atcGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGtt                                               >  7:158431/1‑136 (MQ=255)
                                                                           tcGGCTGTTATAATTTGTGGATTTGTGAGCGGATAACATTTTAACACAAAAACATAGTGTTTAGTAGGAATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCGGGGGGCAACACCGTTGAAATGTTg                                              <  8:32888/136‑1 (MQ=255)
                                                                           tcGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTg                                              <  1:267208/136‑1 (MQ=255)
                                                                           tcGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTg                                              <  4:166253/136‑1 (MQ=255)
                                                                           tcGGATCGTATAATGTGTGTAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTg                                              <  7:123706/136‑1 (MQ=255)
                                                                                  gTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAAc                                        <  2:129006/135‑1 (MQ=255)
                                                                                           gtgGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTg                              >  6:182909/1‑136 (MQ=255)
                                                                                            tgGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAgc                                                                                                       <  5:101451/62‑1 (MQ=255)
                                                                                            tgGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAgc                                                                                                       >  6:101451/1‑62 (MQ=255)
                                                                                            tgGAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTgg                             >  3:358362/1‑136 (MQ=255)
                                                                                             ggAATTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGgc                            >  3:334131/1‑136 (MQ=255)
                                                                                               aaTTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGAt                                <  5:30607/130‑1 (MQ=255)
                                                                                               aaTTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGAt                                >  6:30607/1‑130 (MQ=255)
                                                                                               aaTTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGgcgc                          >  3:174078/1‑136 (MQ=255)
                                                                                               aaTTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGgcgc                          >  7:29178/1‑136 (MQ=255)
                                                                                               aaTTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGgcgc                          >  1:178299/1‑136 (MQ=255)
                                                                                               aaTTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGgcgc                          >  5:269952/1‑136 (MQ=255)
                                                                                               aaTTGTGAGCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGgcgc                          >  3:53170/1‑136 (MQ=255)
                                                                                                       gCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTc                   >  7:282371/1‑135 (MQ=255)
                                                                                                       gCGGATAACAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGAAACACCGTTGAAATGTTGCGTAACTACCAGATTGTCGCCTACGTc                   >  8:120834/1‑135 (MQ=255)
                                                                                                              aCAATTTCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGgt           <  4:137622/136‑1 (MQ=255)
                                                                                                                   ttCACACCAACACATAGAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGtcgtcg      >  3:184312/1‑136 (MQ=255)
                                                                                                                       cacCAACACATATAGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGcc  <  7:34377/136‑1 (MQ=255)
                                                                                                                                    |                                                                                                                          
AGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAGCTGTTGACAATTAATCATCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACACCAACACATAGGGTTTAGGAGGTATACATGAGCGCCCCGACTAACCTGGAGCAGGTGCTGGCCGCCGGCGGCAACACCGTTGAAATGTTGCGTAACTCCCAGATTGGCGCCTACGTCTACCCGGTCGTCGCGCC  >  ACB143/4759377‑4759631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑