Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | ACB143 | 4,882,564 | G→T | 5.9% | Q159H (CAG→CAT) | hyi → | Hydroxypyruvate isomerase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | ACB143 | 4,882,564 | 0 | G | T | 5.9% | 236.7 / 2.4 | 68 | Q159H (CAG→CAT) | hyi | Hydroxypyruvate isomerase |
Reads supporting (aligned to +/- strand): ref base G (33/31); new base T (2/2); total (35/33) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01 |
TGCGCCACCGTGCGTAAGACCTTCGTCGAGAACCTGCGCTACGCCGCTGACAAGCTCAAGGGCGCCGGGATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACCACATGCAGATCATGGAAGGTGACCTGGCTCGCACCATGGAAGCCAACCTGAAGCTGATCAAC > ACB143/4882430‑4882690 | tGCGCCACCGTGCGTAAGACCTTCGTCGAGAACCTGCGCTACGCCGCTGACAAGCTCAAGGGCGCCGGGATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCcag > 5:155180/1‑135 (MQ=255) cACCGTGCGTAAGACCTTCGTCGAGAACCTGCGCTACGCCGCTGACAAGCTCAAGGGCGCCGGGATCCGCCTGGTCATGGAAATGATCAACACCCGCGACCTCCCCGGCTTCTACCTGAACACCACCCAGCAGGcc > 5:146886/1‑136 (MQ=255) cACCGTGCGTAAGACCTTCGTCGAGAACCTGCGCTACGCCGCTGACAAGCTCAAGGGCGCCGGGATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCATCAGGcc > 4:388259/1‑136 (MQ=255) cACCGTGCGTAAGACCTTCGTCGAGAACCTGCGCTACGCCGCTGACAAGCTCAAGGGCGCCGGGATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCATCAGGcc > 4:23220/1‑136 (MQ=255) cACCGTGCGTAAGACCTTCGTCGAGAACCTGCGCTACGCCGCTGACAAGCTCAAGGGCGCCGGGATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGcc > 6:302012/1‑136 (MQ=255) cACCGTGCGTAAGACCTTCGTCGAGAACCTGCGCTACGCCGCTGACAAGCTCAAGGGCGCCGGGATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGcc > 6:253813/1‑136 (MQ=255) cACCGTGCGTAAGACCTTCGTCGAGAACCTGCGCTACGCCGCTGACAAGCTCAAGGGCGCCGGGATCCGCCTGGTCATGGAAATGATCAACACACGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGcc > 7:342652/1‑136 (MQ=255) aCCGTGCGTAAGACCTTCGTCGAGAACCTGCGCTACGCCGCTGACAAGCTCAAGGGCGCCGGGATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGccc > 2:393000/1‑136 (MQ=255) gCGTAAGACCTTCGTCGAGAACCTGCGCTACGCCGCTGACAAGCTCAAGGGCGCCGGGATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGa > 7:44444/1‑135 (MQ=255) aaGACCTTCGTCGAGAACCTGCGCTACGCCGCTGACAAGATCAAGGGCGCCGGGATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATc > 8:371635/1‑135 (MQ=255) cTTCGTCGAGAACCTGCGCTACGCCGCTGACAAGCTCAAGGGCGCCGGGATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGcc > 7:33580/1‑136 (MQ=255) cTTCGTCGAGAACCTGCGCTACGCCGCTGACAAGCTCAAGGGCGCCGGGATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGcc < 8:246968/136‑1 (MQ=255) cTTCGTCGAGAACCTGCGCTACGCCGCTGACAAGCTCAAGGGCGCCGGGATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGcc > 3:65273/1‑136 (MQ=255) cgtcgAGAACCTGCGCTACGCCGCTGACAAGCTCAAGGGCGCCGGGATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCCCCCAGCAGGCCCTGGAAATCCAGGCCg > 6:348827/1‑134 (MQ=255) tcgAGAACCTGCGCTACGCCGCTGACAAGCTCAAGGGCGCCGGGATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGt < 1:365239/136‑1 (MQ=255) tcgAGAACCTGCGCTACGCCGCTGACAAGCTCAAGGGCGCCGGGATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGt < 2:198211/136‑1 (MQ=255) tcgAGAACCTGCGCTACGCCGCTGACAAGCTCAAGGGCGCCGGGATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGt < 5:354103/136‑1 (MQ=255) tcgAGAACCTGCGCTACGCCGCTGACAAGCTCAAGGGCGCCGGGATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGt > 1:81301/1‑136 (MQ=255) tcgAGAACCTGCGCTACGCCGCTGACAAGCTCAAGGGCGCCGGGATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGt < 3:340124/136‑1 (MQ=255) tcgAGAACCTGCGCTACGCCGCTGACAAGCTCAAGGGCGCCGGGATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGt > 3:90807/1‑136 (MQ=255) cgAGAACCTGCGCTACGCCGCTGACAAGCTCAAGGGCGCCGGGATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTg > 2:180042/1‑136 (MQ=255) cTGCGCTACGCCGCTGACAAGCTCAAGGGCGCCGGGATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGc > 7:358412/1‑135 (MQ=255) cgcTACGCCGCTGACAAGCTCAAGGGCGCCGGGATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGa < 6:84575/134‑1 (MQ=255) cgccgcTGACAAGCTCAAGGGCGCCGGGATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTg < 6:131815/136‑1 (MQ=255) cgcTGACAAGCTCAAGGGCGCCGGGATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTc < 2:242658/136‑1 (MQ=255) cgcTGACAAGCTCAAGGACGCCGGGATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTc > 5:123960/1‑136 (MQ=255) aCAAGCTCAAGGGCGCCGGGATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGc > 3:366112/1‑135 (MQ=255) aCAAGCTCAAGGGCGCCGGGATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGc < 1:17590/135‑1 (MQ=255) cAAGCTCAAGGGCGCCGGGATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCaa < 2:311647/136‑1 (MQ=255) aaGCTCAAGGGCGCCGGGATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAAt < 2:363165/136‑1 (MQ=255) aaGGGCGCCGGGATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGa < 5:282408/134‑1 (MQ=255) aaGGGCGCCGGGATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTACTGCAATACTACa > 4:109424/1‑136 (MQ=255) ggCGCCGGGATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCt < 8:358412/136‑1 (MQ=255) cGGGATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCATCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACcac < 3:388259/136‑1 (MQ=255) cGGGATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCATCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACcac < 3:23220/136‑1 (MQ=255) cGGGATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACcac < 4:49747/136‑1 (MQ=255) cGGGATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACcac < 8:282267/136‑1 (MQ=255) cGGGATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACcac < 5:344728/136‑1 (MQ=255) cGGGATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACcac < 1:17392/136‑1 (MQ=255) ggATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACCACAt > 6:198633/1‑136 (MQ=255) ggATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACCACAt > 7:235355/1‑136 (MQ=255) ggATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACCACAt > 6:177949/1‑136 (MQ=255) ggATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACCACAt > 6:157508/1‑136 (MQ=255) ggATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACCACAt > 3:403016/1‑136 (MQ=255) ggATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACCACAt > 7:74777/1‑136 (MQ=255) ggATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACCACAt > 8:124854/1‑136 (MQ=255) ggATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACCACAt > 1:72196/1‑136 (MQ=255) gATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACCACATg < 8:364965/136‑1 (MQ=255) gATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACCACATg < 2:30853/136‑1 (MQ=255) gTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACCACATGCAGATCATgg < 6:199931/136‑1 (MQ=255) gAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACCACATGCAGATCATGGAAGGt < 3:153104/135‑1 (MQ=255) gAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACCACATGCAGATCATGGAAGGt < 5:302012/135‑1 (MQ=255) gAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACCACATGCAGATCATGGAAGGt < 6:7829/135‑1 (MQ=255) gAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACCACATGCAGATCATGGAAGGt < 7:163995/135‑1 (MQ=255) aaTGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACCACATGCAGATCATGGAAGGTGAc > 6:94846/1‑136 (MQ=255) tCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACCACATGCAGATCATGGAAGGTGACCTGGc > 7:62356/1‑136 (MQ=255) ccGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACCACATGCAGATCATGGAAGGTGACCTGGCTCGCAc > 3:183418/1‑135 (MQ=255) aCATCCCCGGCTTCTACCTGATCACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACCACATGCAGATCATGGAAGGTGACCTGGCTCGCACCATGGa < 1:105961/136‑1 (MQ=255) aCATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACCACATGCAGATCATGGAAGGTGACCTGGCTCGCACCATGGa < 3:9613/136‑1 (MQ=255) aCATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACCACATGCAGATCATGGAAGGTGACCTGGCTCGCACCATGGa < 2:39122/136‑1 (MQ=255) cATCCCAGGCTTCTACCTGAACACCAACCAGCAGGCCCTGGAAATCCATGCCGAAGTTGGCAGCGACAACCTGTTCCTTCAATACGACATCTACCACATGCAGATCATGGAAGGTGACCTGGCTCGCACCATGGa < 6:47873/135‑1 (MQ=255) ccGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACCACATGCAGATCATGGAAGGTGACCTGGCTCGCACCATGGAAGCCaa > 3:36999/1‑136 (MQ=255) tCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACCACATGCAGATCATGGAAGGTGACCTGGCTCGCACCATGGAAGCCAACCTg > 4:309395/1‑134 (MQ=255) tCTACCTGAAAACCACCCAGAAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACATGTTCCTGCAATACGACATCTACCACATGCAGATCATGGAAGGTGCCCTGGCTCGCACCATGGAAGCCAACCTg > 5:251867/1‑134 (MQ=255) ccTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACCACATGCAGATCATGGAAGGTGACCTGGCTCGCACCATGGAAGCCAACCTGAAGCt < 5:77093/135‑1 (MQ=255) ccTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACCACATGCAGATCATGGAAGGTGACCTGGCTCGCACCATGGAAGCCAACCTGAAGCt < 5:200956/135‑1 (MQ=255) accaccCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACCACATGCAGATCATGGAAGGTGACCTGGCTCGCACCATGGAAGCCAACCTGAAGCTGATCAAc > 1:60843/1‑135 (MQ=255) ccAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGa < 1:291762/60‑1 (MQ=255) ccAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGa > 2:291762/1‑60 (MQ=255) | TGCGCCACCGTGCGTAAGACCTTCGTCGAGAACCTGCGCTACGCCGCTGACAAGCTCAAGGGCGCCGGGATCCGCCTGGTCATGGAAATGATCAACACCCGCGACATCCCCGGCTTCTACCTGAACACCACCCAGCAGGCCCTGGAAATCCAGGCCGAAGTGGGCAGCGACAACCTGTTCCTGCAATACGACATCTACCACATGCAGATCATGGAAGGTGACCTGGCTCGCACCATGGAAGCCAACCTGAAGCTGATCAAC > ACB143/4882430‑4882690 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |