Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 5,454,155 C→A 5.2% F234L (TTC→TTA)  leuS → Leucine‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,454,1550CA 5.2% 274.8 / 3.0 77F234L (TTC→TTA) leuSLeucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (49/24);  new base A (2/2);  total (51/26)
Fisher's exact test for biased strand distribution p-value = 6.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

CTACTTCCGCATCACCGATTACGCCGACGAGCTGCTGGAGAGCCTTGACGAGCTGCCGGGCTGGCCTGAGCAGGTCAAGACCATGCAGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCACCTATGTCGCCGTCGCCGCCGAGCACCCGCTGGCCACCCAGGCTGCGCAGGGCA  >  ACB143/5454026‑5454288
                                                                                                                                 |                                                                                                                                     
ctactTCCGCATCACCGATTACGCCGACGAGCTGCTGGAGAGCCTTGACGAGCTGCCGGGCTGGCCTGAGCAGGTCAAGACCATGCAGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTAc                                                                                                                                 <  7:41196/136‑1 (MQ=255)
ctactTCCGCATCACCGATTACGCCGACGAGCTGCTGGAGAGCCTTGACGAGCTGCCGGGCTGGCCTGAGCAGGTCAAGACCATGCAGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTAc                                                                                                                                 <  5:278278/136‑1 (MQ=255)
ctactTCCGCATCACCGATTACGCCGACGAGCTGCTGGAGAGCCTTGACGAGCTGCCGGGCTGGCCTGAGCAGGTCAAGACCATGCAGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTAc                                                                                                                                 <  5:128348/136‑1 (MQ=255)
ctactTCCGCATCACCGATTACGCCGACGAGCTGCTGGAGAGCCTTGACGAGCTGCCGGGCTGGCCTGAGCAGGTCAAGACCATGCAGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTAc                                                                                                                                 <  2:118383/136‑1 (MQ=255)
   ctTCCGCATCACCGATTACGCCGACGAGCTGCTGGAGAGCCTTGACGAGCTGCCGGGCTGGCCTGAGCAGGTCAAGACCATGCAGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGa                                                                                                                               >  8:88254/1‑135 (MQ=255)
      ccGCATCACCGATTACGCCGACGAGCTGCTGGAGAGCCTTGACGAGCTGCCGGGCTGGCCTGAGCAGGTCAAGACCATGCAGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAg                                                                                                                           >  5:142834/1‑136 (MQ=255)
      ccGCATCACCGATTACGCCGACGAGCTGCTGGAGAGCCTTGACGAGCTGCCGGGCTGGCCTGAGCAGGTCAAGACCATGCAGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAg                                                                                                                           >  5:326957/1‑136 (MQ=255)
         cATCACCGATTACGCCGACGAGCTGCTGGAGAGCCTTGACGAGCTGCCGGGCTGGCCTGAGCAGGTCAAGACCATGCAGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGc                                                                                                                         >  4:180682/1‑135 (MQ=255)
         cATCACCGATTACGCCGACGAGCTGCTGGAGAGCCTTGACGAGCTGCCGGGCTGGCCTGAGCAGGTCAAGACCATGCAGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGc                                                                                                                         >  3:352697/1‑135 (MQ=255)
          aTCACCGATTACGCCGACGAGCTGCTGGAGAGCCTTGACGAGCTGCCGGGCTGGCCTGAGCAGGTCAAGACCATGCAGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCa                                                                                                                       <  4:178386/136‑1 (MQ=255)
            cACCGATTACGCCGACGAGCTGCTGGAGAGCCTTGACGAGCTGCCGGGCTGGCCTGAGCAGGTCAAGACCATGCAGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAg                                                                                                                      >  1:72295/1‑135 (MQ=255)
                       ccGACGAGCTGCTGGAGAGCCTTGACGAGCTGCCGGGCTGGCCGGAGCAGGTCAAGACCATGCAGCGCAACTGGCTCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACACGGCCAGCATCGGCCACGa                                                                                                          <  7:226981/136‑1 (MQ=255)
                         gacgaGCTGCTGGAGAGCCTTGACGAGATGCCGGGCTGGCCTGAGCAGGTCAAGACCATGCAGCGCAACTGTATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAg                                                                                                        <  1:306251/136‑1 (MQ=255)
                               ctgctgGAGAGCCTTGACGAGCTGCCGGGCTGGCCTGAGCAGGTCAAGACCATGCAGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCAccc                                                                                                  <  4:29906/136‑1 (MQ=255)
                                           cTTGACGAGCTGCCGGGCTGGCCTGAGCAGGTCAAGACCATGCAGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTca                                                                                      >  6:266190/1‑136 (MQ=255)
                                             tGACGAGCTGCCGGGCTGGCCTGAGCAGGTCAAGACCATGCAGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTcacc                                                                                    >  7:269054/1‑136 (MQ=255)
                                             tGACGAGCTGCCGGGCTGGCCTGAGCAGGTCAAGACCATGCAGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTcac                                                                                     >  1:39027/1‑135 (MQ=255)
                                                cGAGCTGCCGGGCTGGCCTGAGCAGGTCAAGACCATGCAGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCaccacc                                                                                 >  2:119644/1‑136 (MQ=255)
                                                       ccGGGCTGGCCTGAGCAGGTCAAGACCATGCAGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGa                                                                                                          <  3:278853/104‑1 (MQ=255)
                                                       ccGGGCTGGCCTGAGCAGGTCAAGACCATGCAGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGa                                                                                                          >  4:278853/1‑104 (MQ=255)
                                                       ccGGGCTGGCCTGAGCAGGTCAAGACCATGCAGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCgg                                                                          >  4:111221/1‑136 (MQ=255)
                                                         gggCTGGCCTGAGCAGGTCAAGACCATGCAGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGac                                                                        <  4:156033/136‑1 (MQ=255)
                                                                  tGAGCAGGTCAAGACCATGCAGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATg                                                               >  4:387956/1‑136 (MQ=255)
                                                                    aGCAGGTCAAGACCATGCAGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATggg                                                             <  6:77004/136‑1 (MQ=255)
                                                                    aGCAGGTCAAGACCAGGCAGCGCAAATGTATCGGCATATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACACGGCCAGCATCGGCCACGAAGTCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATgg                                                              <  6:24909/135‑1 (MQ=255)
                                                                       aGGTCAAGACCATGCAGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGgcgc                                                          >  4:193867/1‑136 (MQ=255)
                                                                       aGGTCAAGACCATGCAGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGgcgc                                                          >  7:169636/1‑136 (MQ=255)
                                                                       aGGGCATGACGAAGCAGGGCAACTGGATCGGCATATCGGGCGGCAGGGAAGTCGAGTTCCCGTACGAAAACGCCACCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGgcgc                                                          <  6:385169/136‑1 (MQ=255)
                                                                            aaGACCATGCAGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGATAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCAcc                                                      >  1:156405/1‑135 (MQ=255)
                                                                            aaGACCATGCAGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCACGGTCTTCACCCCCAGTCCGGACACGCTGATGGGCGCCACCt                                                     >  4:33204/1‑136 (MQ=255)
                                                                            aaGACCATGCAGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCCCCACCCGTCCGGACACGCTGATGGGCGCCAcc                                                      >  3:358108/1‑135 (MQ=255)
                                                                            aaGACCATGCAGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCAcc                                                      >  3:391219/1‑135 (MQ=255)
                                                                            aaGACCATGCAGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCAcc                                                      >  3:346181/1‑135 (MQ=255)
                                                                            aaGACCATGCAGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCAcc                                                      >  7:123433/1‑135 (MQ=255)
                                                                            aaGACCATGCAGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCAGTCCGGCCACGCTGATGGGCGCCAcc                                                      >  4:38813/1‑135 (MQ=255)
                                                                                 cATGCAGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCACCTATgtc                                                >  6:252395/1‑136 (MQ=255)
                                                                                 cATGCAGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCACCTATgtc                                                >  2:375431/1‑136 (MQ=255)
                                                                                   tGCAGCGCAAGTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCACCTATgtcgc                                              <  8:233268/136‑1 (MQ=255)
                                                                                   tGCAGCGCAACTGGATCGGAAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCACCTATgtcgc                                              <  4:269685/136‑1 (MQ=255)
                                                                                      aGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGCCAAGGCCAGCATCGGCCACGAAGGCCCCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCACCTATgtcgccgt                                           >  1:366042/1‑136 (MQ=255)
                                                                                      aGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCCGCATCGGCCACGAAGGCACCCTCAAGGACTTCACCACCCGTCCGGACACGCTGCTGGGCGCCACCTATgtcgccgt                                           >  6:215534/1‑136 (MQ=255)
                                                                                      aGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCCCCCGTCCGGACACGCTGATGGGCGCCACCTATgtcgccgt                                           >  5:182445/1‑136 (MQ=255)
                                                                                      aGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCCCCTATgtcgccgt                                           >  1:167595/1‑136 (MQ=255)
                                                                                      aGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCCCCTATgtcgccgt                                           >  7:37367/1‑136 (MQ=255)
                                                                                      aGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCCCCTATgtcgccgt                                           >  5:205835/1‑136 (MQ=255)
                                                                                      aGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCACCTATgtcgccgt                                           >  2:23899/1‑136 (MQ=255)
                                                                                      aGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCACCTATgtcgccgt                                           >  3:296673/1‑136 (MQ=255)
                                                                                      aGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCACCTATgtcgccgt                                           >  8:341008/1‑136 (MQ=255)
                                                                                      aGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCACCTATgtcgccgt                                           >  1:339796/1‑136 (MQ=255)
                                                                                      aGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCACCTATgtcgccgt                                           >  1:124879/1‑136 (MQ=255)
                                                                                      aGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCACCTATgtcgccgt                                           >  1:167402/1‑136 (MQ=255)
                                                                                      aGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCACCTATgtcgccgt                                           >  6:358556/1‑136 (MQ=255)
                                                                                      aGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTACCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCACCTATgtcgccgt                                           >  2:379538/1‑136 (MQ=255)
                                                                                      aGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTACCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCACCTATgtcgccgt                                           >  2:385058/1‑136 (MQ=255)
                                                                                           aaCTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCACCTATGtcgccgtcgccg                                      >  2:94472/1‑136 (MQ=255)
                                                                                              tGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCACCTATGTCGCCGTCGccgccg                                   >  4:376135/1‑136 (MQ=255)
                                                                                                gATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTcac                                                                                     <  5:327241/84‑1 (MQ=255)
                                                                                                gATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTcac                                                                                     >  6:327241/1‑84 (MQ=255)
                                                                                                gATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCACCTATGTCGCCGTCGCCGCCGAg                                 >  1:84963/1‑136 (MQ=255)
                                                                                                     gCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCACCTATGTCGCCGTCGCCGCCGAGCAccc                            >  8:265974/1‑136 (MQ=255)
                                                                                                      cAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCACCTATGTCGCCGTCGCCGCCGAGCACCCg                           <  3:49883/136‑1 (MQ=255)
                                                                                                      cAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCACCTATGTCGCCGTCGCCGCCGAGCACCCg                           <  5:262855/136‑1 (MQ=255)
                                                                                                        aaTCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCACCTATGTCGCCGTCGCCGCCGAGCACCCGCt                         >  6:312793/1‑136 (MQ=255)
                                                                                                        aaTCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCACCTATGTCGCCGTCGCCGCCGAGCACCCGCt                         >  7:292837/1‑136 (MQ=255)
                                                                                                        aaTCGCGCGGCATGGAAGTCCAGTTACCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCACCTATGTCGCCGTCGCCGCCGAGCACCCGCt                         <  1:379538/136‑1 (MQ=255)
                                                                                                        aaTCGCGCGGCATGGAAGTCCAGTTACCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCACCTATGTCGCCGTCGCCGCCGAGCACCCGCt                         <  1:385058/136‑1 (MQ=255)
                                                                                                            gcgcgGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCACCTATGTCGCCGTCGCCGCCGAGCACCCGCTGGc                      <  8:99548/135‑1 (MQ=255)
                                                                                                             cgcgGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCACCTATGTCGCCGTCGCCGCCGAGCACCCGCTGGCCa                    <  7:315299/136‑1 (MQ=255)
                                                                                                                ggCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCACCTATGTCGCCGTCGCCGCCGAGCACCCGCTGGCCAccc                 <  3:75428/136‑1 (MQ=255)
                                                                                                                ggCATGGAAGTCCAGGTCCCGTACGACAAGTCCACCATCGGCCACGAAGGCACCCTCAAGCTCATCACCACCCGTCCCGACACGCTGATGGGCGCCACCTATGTCGCCGTCGCCGCCGAGCACCCGCTGGCCAccc                 <  8:98507/136‑1 (MQ=255)
                                                                                                                     ggAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCTACCGGACACGCTGATGTGCGCCACCTATGTCGCCGTCGCCGCCGAGCACCCGCTGGCCACCCAGGCt            <  7:386548/136‑1 (MQ=255)
                                                                                                                      gAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCACCTATGTCGCCGTCGCCGCCGAGCACCCGCTGGCCACCCAGGCt            <  3:111221/135‑1 (MQ=255)
                                                                                                                               ttCCCGTACTCCAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTACACAACCCGTCCGGACACGCTGAAGGGCGCCAACTATGTCGCCCTCGCCGCCGAGCACCCGCTGGCCACACAGGCTGCTCAGGGc   >  6:354311/1‑135 (MQ=255)
                                                                                                                               ttCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCACCTATGTCGCCGTCGCCGCCGAGCACCCGCTGGCCACCCAGGCTGCGCAGGGc   >  1:214712/1‑135 (MQ=255)
                                                                                                                               ttCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCACCTATGTCGCCGTCGCCGCCGAGCACCCGCTGGCCACCCAGGCTGCGCAGGGc   >  3:314864/1‑135 (MQ=255)
                                                                                                                               ttCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCACCTATGTCGCCGTCGCCGCCGAGCACCCGCTGGCCACCCAGGCTGCGCAGGGc   >  3:316743/1‑135 (MQ=255)
                                                                                                                               ttCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCACCTATGTCGCCGTCGCCGCCGAGCACCCGCTGGCCACCCAGGCTGCGCAGGGCa  >  4:331121/1‑136 (MQ=255)
                                                                                                                                 |                                                                                                                                     
CTACTTCCGCATCACCGATTACGCCGACGAGCTGCTGGAGAGCCTTGACGAGCTGCCGGGCTGGCCTGAGCAGGTCAAGACCATGCAGCGCAACTGGATCGGCAAATCGCGCGGCATGGAAGTCCAGTTCCCGTACGACAAGGCCAGCATCGGCCACGAAGGCACCCTCAAGGTCTTCACCACCCGTCCGGACACGCTGATGGGCGCCACCTATGTCGCCGTCGCCGCCGAGCACCCGCTGGCCACCCAGGCTGCGCAGGGCA  >  ACB143/5454026‑5454288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑