Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB143 2,031,417 (G)10→9 coding (1120/1143 nt) wecB ← UDP‑N‑acetylglucosamine 2‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,031,4080G.100.0% 17.4 / NA 5coding (1129/1143 nt)wecBUDP‑N‑acetylglucosamine 2‑epimerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (5/0);  total (5/0)

GCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGCGGTGGGGGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCA  >  ACB143/2031312‑2031536
                                                                                                |                                                                                                                                
gCACGAGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGAGGGGCTGCGGATATCGTATTCGAGGGTGAACCCTCACCTAAATTGAGAGGTGGGGGGGGGCCAGGTGGCCGGGGATTTGCGTTTgcgcgcg                                                                                          >  8:323391/1‑136 (MQ=255)
  aCGCGGAAGGGGGCCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCAACATTCACCGGTGGGGGGGGCCCAGGAGGCCAGGGCTTTGCGAATgcgcgac                                                                                          >  2:84726/1‑132 (MQ=255)
                                                 cTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGCGGTGGGGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGa                                          >  8:269591/1‑135 (MQ=255)
                                                                            ccGCACCTACATTGAGCGGTGGGGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTAc              >  1:107286/1‑136 (MQ=255)
                                                                                        tGAGCGGTGGGGGGGGGCCAGGTGGCCAGGGCTTTGCGTAAGCGCGCGCAGGCTTTGCCATCGCCATACGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGca  >  3:341972/1‑136 (MQ=255)
                                                                                                |                                                                                                                                
GCACGCGGAAGGGGGGCTGCTGGCCAGTTTTTACTGGGGGGCGGCGGGGCTGCGGATATCGTATTCGCGGGTGAACCCGCACCTACATTGAGCGGTGGGGGGGGGGCCAGGTGGCCAGGGCTTTGCGTATGCGCGCGCAGGCTTTGCCATCGCCATAGGGGTTATGGGCGAAGCTCATTTCGCGATACGCCGCCTCATCGGTCAGCAAGGTACTCAACGCCCGCA  >  ACB143/2031312‑2031536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: