Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB143 120,983 (T)9→8 intergenic (+280/+92) polA → / ← engB DNA polymerase I/putative GTP‑binding protein EngB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB143120,9750T.94.9% 251.4 / 6.7 59intergenic (+272/+100)polA/engBDNA polymerase I/putative GTP‑binding protein EngB
Reads supporting (aligned to +/- strand):  ref base T (0/3);  new base . (42/14);  total (42/17)
Fisher's exact test for biased strand distribution p-value = 2.09e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTAACTGAATGGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGTTCCATC  >  ACB143/120847‑121105
                                                                                                                                |                                                                                                                                  
gTAACTGAATGGCTGGTGAAGCCCTTCGATTCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCAGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGtttttttt                                                                                                                             <  3:360156/136‑1 (MQ=255)
       aaTGGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCgg                                                                                                                      >  2:119250/1‑135 (MQ=255)
          ggCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAAtt                                                                                                                  >  6:153197/1‑136 (MQ=255)
           gCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAAttt                                                                                                                 <  2:296328/136‑1 (MQ=255)
                            aTGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGACAGGAATTTGCCGGGAGCGGGTTTtc                                                                                                >  1:213182/1‑136 (MQ=255)
                             tGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGACGGGAGCGGGTTTtc                                                                                                >  3:24635/1‑135 (MQ=255)
                                  cTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTcgcg                                                                                           >  7:10953/1‑135 (MQ=255)
                                  cTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGa                                                                                          <  5:15432/136‑1 (MQ=255)
                                    aTGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt                                                                                        >  4:27087/1‑136 (MQ=255)
                                     tgttgtGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTATCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt                                                                                        <  2:160423/135‑1 (MQ=255)
                                     tgttgtGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt                                                                                        <  1:114110/135‑1 (MQ=255)
                                     tggtgtgTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt                                                                                        <  3:104269/132‑1 (MQ=255)
                                      gttgtGTTAACGGTTGGCACAAAACCTGCCCCCGCCCCAGCCGGCCGGAAATGGACCCCGAACATCCCCCACCCCAAACCCAGGCCCGGGTTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt                                                                                        <  4:347595/135‑1 (MQ=255)
                                       ttgtGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGc                                                                                      <  5:112322/135‑1 (MQ=255)
                                            ttAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTc                                                                                >  2:267077/1‑136 (MQ=255)
                                             tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCTGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCCATGTc                                                                                >  5:97676/1‑135 (MQ=255)
                                             tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTc                                                                                >  6:32370/1‑135 (MQ=255)
                                             tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTc                                                                                >  2:172604/1‑135 (MQ=255)
                                             tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTc                                                                                >  2:75148/1‑135 (MQ=255)
                                             tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTc                                                                                >  4:119282/1‑135 (MQ=255)
                                             tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTc                                                                                >  2:13533/1‑135 (MQ=255)
                                             tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTc                                                                                >  5:43103/1‑135 (MQ=255)
                                             tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTc                                                                                >  3:4002/1‑135 (MQ=255)
                                             tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTc                                                                                >  8:309853/1‑135 (MQ=255)
                                             tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCAGGGAGCGGGTTTTCTCGCGAATGCGATGTc                                                                                >  1:16450/1‑135 (MQ=255)
                                             tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCACCCAATACGAAGTCCGGGGTATTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGAtctc                                                                                >  4:114374/1‑135 (MQ=255)
                                             tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTACGTACTTGGACCACGAACTTCCCCATCCCCATACGAAGTCCGGGGTTTTTTTTGACCGGAATTTGCCTGGAGCGGGTTTTCTCGCGAAGGCGATGTc                                                                                >  6:54855/1‑135 (MQ=255)
                                               aGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGaa                                                                             >  3:268564/1‑136 (MQ=255)
                                               aGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGaa                                                                             >  8:301270/1‑136 (MQ=255)
                                               aGTGTTGGCAGATATCTGGACCCCGCCCTAGCCGTCCGGACTTGGACCCCCAACTTCCCCCTCCCCATACGAAGACCGGGGTTTTTTTTGCCCGGAATTTGCCGGGACCGGGTTTTCTCGCGAATGCGATGTCGaa                                                                             >  2:77928/1‑136 (MQ=255)
                                                  gtTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATa                                                                           >  3:374077/1‑135 (MQ=255)
                                                       cAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACAccgc                                                                     >  3:35355/1‑136 (MQ=255)
                                                       cAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATAAAccgc                                                                     >  8:257095/1‑136 (MQ=255)
                                                              cTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTc                                                              >  2:116057/1‑136 (MQ=255)
                                                                 gACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTcgcg                                                           <  3:178385/136‑1 (MQ=255)
                                                                    cccGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGt                                                        <  7:4218/136‑1 (MQ=255)
                                                                         ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAAcc                                                   >  1:12218/1‑136 (MQ=255)
                                                                         ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAAcc                                                   >  1:174990/1‑136 (MQ=255)
                                                                         ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAAcc                                                   >  7:108909/1‑136 (MQ=255)
                                                                         ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAAcc                                                   >  1:24042/1‑136 (MQ=255)
                                                                         ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAAcc                                                   >  5:83043/1‑136 (MQ=255)
                                                                         ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAAca                                                   >  2:375607/1‑135 (MQ=255)
                                                                             gCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTGATCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCg                                                 <  6:179837/134‑1 (MQ=255)
                                                                                   cGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGgc                                         <  3:57721/136‑1 (MQ=255)
                                                                                   cGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGgc                                         <  4:268564/136‑1 (MQ=255)
                                                                                   cGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGgc                                         <  7:30913/136‑1 (MQ=255)
                                                                                   cGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGgc                                         <  5:322127/136‑1 (MQ=255)
                                                                                    ggccTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGCAGTTGCCGGGAGCGGGTTTTATCGCGAATGCGATGTCGAATACACCGCCGCATTCTCGGGTAAACCCGGGTCAGgcg                                        >  8:308389/4‑136 (MQ=255)
                                                                                     gACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGgcg                                        >  2:63669/1‑135 (MQ=255)
                                                                                      aCTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGgcgc                                       >  2:311467/1‑135 (MQ=255)
                                                                                      aCTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGgcgc                                       >  7:182048/1‑135 (MQ=255)
                                                                                             ccccGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTACTCCCGGGTCAGGCGCAAACCAc                                >  1:92106/1‑135 (MQ=255)
                                                                                              cccGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACt                               >  7:237333/1‑135 (MQ=255)
                                                                                                   aCTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGa                         >  4:297693/1‑136 (MQ=255)
                                                                                                     ttCCCCCTCCCCATACGAAGTCCGGTGTTTTTTTTGCCCGTAATTTGCCGGGAGCGGGTTTTCTCGCGAATGAGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACAACTAAAAGAcc                       >  1:28216/1‑136 (MQ=38)
                                                                                                        ccccTCCCCATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGc                    <  3:119282/136‑1 (MQ=255)
                                                                                                        ccccTCCCCATACGAAGGCGGGGTTTTTTTTTGCCCGTAATTTGCGGGGAGGGGGTTTTCTCGCTATTGCTATGTCTAATACACCGCCGCATTCGCGGGTAAACCGGGGTCAGGCGCAAACCACTAAACGACCGGc                    <  5:32370/136‑1 (MQ=255)
                                                                                                              cccATACTATGTCCTGGTTTTTTTTTGCCCGTAATTTGCCGGGAGCGGGTTTACTCGCGAATGCGATGTCTAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTc               <  4:35355/135‑1 (MQ=38)
                                                                                                               ccATACGAAGTCCGGGGTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCtt             >  4:273555/1‑136 (MQ=255)
                                                                                                                     gAAGTCCGGGGTTTTTTTTGCCCTGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGtt       >  6:5458/1‑136 (MQ=255)
                                                                                                                            cggggTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGTtccatc  >  2:253453/2‑135 (MQ=255)
                                                                                                                            cggggTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGTtccatc  >  3:120899/2‑135 (MQ=255)
                                                                                                                            cggggTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGTtccatc  >  5:357264/2‑135 (MQ=255)
                                                                                                                            cggggTTTTTTTTGCACGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGTtccatc  >  5:1875/2‑135 (MQ=255)
                                                                                                                                |                                                                                                                                  
GTAACTGAATGGCTGGTGAAGCCCTTCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGTTCCATC  >  ACB143/120847‑121105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: