Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB143 4,575,178 (G)11→10 intergenic (‑154/+17) CJ019_04328 ← / ← glgE2 Glucosamine kinase/Alpha‑1,4‑glucan:maltose‑1‑phosphatemaltosyltran s ferase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,575,1680G.100.0% 70.0 / NA 16intergenic (‑144/+27)CJ019_04328/glgE2Glucosamine kinase/Alpha‑1,4‑glucan:maltose‑1‑phosphatemaltosyltran s ferase 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (15/1);  total (15/1)

CTTTCAATTGCATGCACAGACATCCGAGCTTGCTCGATCCCCCGTAGGAGCGGGTTTACCCGCGAAGATGACACCGCGGTGCCGAGGTGCATGGCACCGGCTTCGCCGGTGTTCGCGGATAAACCCGCTCCTACGGGGGGGGGGGCACAGTCTGAACCTTGTTAACGGGCTTTTTCGATGCGCCAGATGCCGAACGGCAGGTGCCAGGGCTCTATGCGCATCCATTGGGTCTTGCCGTACCACGACCAGCGGTGGCCGT  >  ACB143/4575034‑4575292
                                                                                                                                      |                                                                                                                            
cTTTCAATTGCATGCACAGACATCCGAGCTTGCTCGATCCCCCGTAGGAGCGGGTTTACCCGCGAAGATGACACCGCGGTGCCGAGGTGCATGGCACCGGCTTCGCCGGTGTTAGCGGATAAACCCGCTCCTACgg                                                                                                                             >  8:287978/1‑136 (MQ=255)
             gcaCAGACATCCGAGCTTGCTCGATCCCCCGTAGGAGCGGGTTTACCCGCGAAGATGACACCGCGGTGCCGAGGTGCATGGCACCGGCTTAGCCGGTGTTCGCGGATAAACCCGCTCCTACGGGGGGGGGGCACAg                                                                                                               >  7:234954/1‑136 (MQ=255)
                                  cGATCCCCCGTAGGAGCGGGTTTACCCGCGAAGATGACCCCGCGGTGCCGAGGTGCATGGCACCGGCTTCGCCGGTGTTCGCGGACAAACCCGCTCCGACGGGGGGGGGGCACAGTCTGAACCTTGTTTACGGGCt                                                                                          >  7:62685/1‑136 (MQ=38)
                                                                 aGATGACACCGCGGTGCCGAGGTGCATGGCACCGGCTTCGCCGGTGTTCGCGGATAAACCCGCTCCTACGGGGGGGGGGCACAGTCTTAACCTTGTTAACGGGCTTTTTCGATGCGCCAGATGCCGAACGGCAGGt                                                           >  3:130764/1‑136 (MQ=255)
                                                                  gATGACACCGCGGTGCCGAGGTGCATGGCACCGGCTTCGCCGGTGTTCGCGGATAAACCCGCTCCTACGGGGGGGGGGCACAGTCTGAACCTTGTTAACGGGCTTTTTCGATGCGCCAGATGCCGAACGGCAGGTg                                                          >  7:15568/1‑136 (MQ=255)
                                                                             ggTGCCGAGGTGCATGGCACCGGCTTCGCCGGTGTTCGCGGATAAACCCGCTCCTACGGGGGGGGGGCACAGTCTGAACCTTGTTAACGGGCTTTTTCGATGCGCCAGATGCCGAACg                                                                 >  7:169731/1‑118 (MQ=255)
                                                                             ggTGCCGAGGTGCATGGCACCGGCTTCGCCGGTGTTCGCGGATAAACCCGCTCCTACGGGGGGGGGGCACAGTCTGAACCTTGTTAACGGGCTTTTTCGATGCGCCAGATGCCGAACg                                                                 <  8:169731/118‑1 (MQ=255)
                                                                                    aGGTGCATGGCACCGGCTTCGCCGGTGTTCGCGGATAAACCCGCTCCTACGGGGGGGGGGCACAGTCTGAACCTTGTTAACGGGCTTTTTCGATGCGCCAGATGCCGAACGGCAGGTGCCAGGGGTCTATGCGCAt                                        >  7:239808/1‑136 (MQ=255)
                                                                                    aGGTGCATGGCACCGGCTTCGCCGGTGTTCGCGGATAAACCCGCTCCTACGGGGGGGGGGCACAGTCTGAACCTTGTTAACGGGCTTTTTCGATGCGCCAGATGCCGAACGGCAGGTGCCAGGGCTCTATGCGCa                                         >  8:236411/1‑135 (MQ=255)
                                                                                         cATGGCACCGGCTTCGCCGGTGTTCGCGGATAAACCCGCTCCTACGGGGGGGGGGCAAAGGAGGACCCTTGGTAACGGGCTTTTTCGATGCGCCCGATGCCGAACGGCAGGTGCCAGGGCTCTATGCGCATCCAtt                                   >  5:215478/1‑136 (MQ=37)
                                                                                            ggCACCGGCTTCGCCGGTGTTCGCGGATAAACCCGCTCCTACGGGGGGGGGGCACAGTATGACCCTTGTTAACGGGCTTTTTCGATGCGCCAGATGCCGAACGGCAGGTGCCAGGGCTCTATGCGCATCCATTggg                                >  8:240291/1‑136 (MQ=255)
                                                                                              cACCGGCTTCGCCGGTGTTCGCGGATAAACCCGCTCCTACGGGGGGGGGGCACAGTCTGAACCTTGTTAACGGGCTTTTTCGATGCGCCAGATGCCGAACGGCAGGTGCCAGGGCTCTATGCGCATCCATTGGGTc                              >  8:225407/1‑136 (MQ=255)
                                                                                                    cTTCGCCGGTGTTCGCGGATAAACCCGCTCCTACGGGGGGGGGGCACAGTCTGAACCTTGTTAACGGGCTTTTTCGATGCGCCAGATGCCGAACGGCAGGTGCCAGGGCTCTATGCGCATCCATTGGGTCTTGCCg                        >  6:142809/1‑136 (MQ=255)
                                                                                                    cTTCGCCGGTGTTCGCGGATAAACCCGCTCCTACGGGGGGGGGGCACAGTCTGAACCTTGTTAACGGGCTTTTTCGATGCGCCAGATGCCGAACGGCAGGTGCCAGGGCTCTATGCGCATCCATTGGGTCTTGCCg                        >  2:16548/1‑136 (MQ=255)
                                                                                                      tCGCCGGTGTTCGCGGATAAACCCGCTCCTACGGGGGGGGGGCACAGTCAGAACCGTGTTAACGGGCTTTATCGATGCGCCAGATGCCGAACGGCAGGTGCCAGGGCGCTATGCGCATCCATTGGGTCTTGCCGTa                      >  3:92846/1‑136 (MQ=255)
                                                                                                                          aCCCGCTCCTACGGGGGGGGGGCACAGTGTGAACCTTGTTAACGGGCTTTTTCGATGCGCCAGATGCCGAACGGCAGGTGCCAGGGGTCTATGCGCATCCATTGGGTCTTGCCGTACCCCGCCCAGCGGTGGCCGt  >  7:194216/1‑136 (MQ=255)
                                                                                                                          aCCCGCTCCTACGGGGGGGGGGCACAGTCTGAACCTTGTTAACGGGCTTTTTCGATGCGCCAGATGCCGAACGGCAGGTGCCAGGGCTCTATGCGCATCCATTGGGTCTTGCCGTACCACGACCAGCGGTGGCCGt  >  7:116649/1‑136 (MQ=255)
                                                                                                                                      |                                                                                                                            
CTTTCAATTGCATGCACAGACATCCGAGCTTGCTCGATCCCCCGTAGGAGCGGGTTTACCCGCGAAGATGACACCGCGGTGCCGAGGTGCATGGCACCGGCTTCGCCGGTGTTCGCGGATAAACCCGCTCCTACGGGGGGGGGGGCACAGTCTGAACCTTGTTAACGGGCTTTTTCGATGCGCCAGATGCCGAACGGCAGGTGCCAGGGCTCTATGCGCATCCATTGGGTCTTGCCGTACCACGACCAGCGGTGGCCGT  >  ACB143/4575034‑4575292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: