Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB143	4,713,495	Δ2 bp	coding (1667‑1668/1905 nt)	htpG ←	Chaperone protein HtpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	4,713,494	0	T	.	100.0%	167.1 / NA	36	coding (1669/1905 nt)	htpG	Chaperone protein HtpG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (14/22);  total (14/22)
*	ACB143	4,713,495	0	C	.	100.0%	167.1 / NA	36	coding (1668/1905 nt)	htpG	Chaperone protein HtpG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (14/22);  total (14/22)

TGTGCGACAGCTCGGCGAAACGGTCTTCGCTCTGCTCGTGATCCAGCTTCTCGATCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCATCTGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCGGCCACGCTGTCACCCAGCGCACCTTTCAGTCGCTCGACCA  >  ACB143/4713359‑4713610                                                                                                                                        ||                                                                                                                    tgtgCGACAGCTCGGCGAAACGGTCTTCGCTCTGCTCGTGATCCAGCTTCTCGATCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCAt                                                                                                                      <  7:75925/136‑1 (MQ=255) tgtgCGACAGCTCGGCGAAACGGTCTTCGCTCTGCTCGTGATCCAGCTTCTCGATCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCAt                                                                                                                      <  8:27228/136‑1 (MQ=255) tgtgCGACAGCTCGGCGAAACGGTCTTCGCTCTGCTCGTGATCCAGCTTCTCGATCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCAt                                                                                                                      <  3:17523/136‑1 (MQ=255)     cGACAGCGCGGCGAAACGGTCTTCGCTCTGCTCGTGAGCCAGCTTCTCGATCAGCGGGTGGCTCGGGCTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCAtgcag                                                                                                                  <  4:95265/136‑5 (MQ=255)      gACAGCTCGGCGAAACGGTCTTCGCTCTGCTCGTGATCCAGCTTCTCGATCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCAtgcagg                                                                                                                 <  2:17361/136‑6 (MQ=255)           cTCGGCGAAACGGTCTTCGCTCTGCTCGTGATCCAGCTTCTCGATCAGCGGGTTGCTCGGGTTGAACTCGAATCTCGGCGTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAg                                                                                                          <  3:313696/136‑1 (MQ=255)           cTCGGCGAAACGGTCTTCGCTCTGCTCGTGATCCAGCTTCTCGATCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAg                                                                                                          <  8:196273/136‑1 (MQ=255)              ggCGAAACGGTCTTCGCTCTGCTCGTGATCCAGCTTCTCGATCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCAtgcagg                                                                                                                 <  8:99243/128‑6 (MQ=255)              ggCGAAACGGTCTTCGCTCTGCTCGTGATCCAGCTTCTCGATCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCAtgcagg                                                                                                                 >  7:99243/1‑123 (MQ=255)              ggCGAAACGGTCTTCGCTCTGCTCGTGATCCAGCTTCTCGATCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTc                                                                                                       >  8:241392/1‑136 (MQ=255)                cGAAACGGTCTTCGCTCTGCTCGTGATCCAGCTTCTCGATCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCt                                                                                                     <  5:89706/136‑1 (MQ=255)                     cGGTCTTCGCTCTGCTCGTGATCCAGCTTCTCGATCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCg                                                                                                <  3:111063/136‑1 (MQ=255)                     cGGTCTTCGCTCTGCTCGTGATCCAGCTTCGCGATCAGCGGGTGGCTCGGGTTGAAAGCGAAGATCGGCTTTGACTCCGGCACTTACTGCCCGCTGGCTGCCAGGATCTGGCGCA‑‑TGCAGGCCCAGTGCCTGCTCg                                                                                                <  6:251787/136‑1 (MQ=255)                        tCTTCGCTCTGCTCGTGATCCAGCTTCTCGATCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGcc                                                                                              >  7:225671/1‑135 (MQ=255)                                      gTGATCCAGCTTCTCGATCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGc                                                                               >  8:114533/1‑136 (MQ=255)                                         atgcAGCTTCTCGATCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCgg                                                                            <  8:342896/133‑1 (MQ=255)                                         aTCCAGCTTCTCGATCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCgg                                                                            <  4:313073/136‑1 (MQ=255)                                         aTCCAGCTTCTCGATAAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGCCTCCGGCACTTTCTGCCCGATGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCgg                                                                            >  6:189252/1‑136 (MQ=255)                                            cAGCTTCTCGATCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCg                                                                          <  8:244410/135‑1 (MQ=255)                                                     gATCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAg                                                                >  5:346299/1‑136 (MQ=255)                                                      aTCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAgg                                                               >  7:150170/1‑136 (MQ=255)                                                          gCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGt                                                           >  8:49132/1‑136 (MQ=255)                                                                        ttGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACt                                             >  3:120688/1‑136 (MQ=255)                                                                          gAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGTCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGAGAGACGCGCACTTc                                           >  2:299315/1‑136 (MQ=255)                                                                           aaCTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTc                                           <  5:77382/135‑1 (MQ=255)                                                                           aaCTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTc                                           <  2:241970/135‑1 (MQ=255)                                                                            aCTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCgg                                         <  3:351717/136‑1 (MQ=255)                                                                            aCTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCgg                                         <  2:252499/136‑1 (MQ=255)                                                                              tCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCGGc                                        >  7:30410/1‑135 (MQ=255)                                                                              tCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCGGc                                        >  8:203199/1‑135 (MQ=255)                                                                                      cGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCGGCCACGCTGTc                               <  1:193788/136‑1 (MQ=255)                                                                                      cGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCGGCCACGCTGTc                               <  2:112123/136‑1 (MQ=255)                                                                                            ggACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCGGCCACGCTGTCACCCa                          <  6:346299/135‑1 (MQ=255)                                                                                            ggACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCGGCCACGCTGTCACCCa                          <  7:291314/135‑1 (MQ=255)                                                                                            ggACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCGGCCACGCTGTCACCCa                          <  2:47525/135‑1 (MQ=255)                                                                                            ggACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCGGCCACGCTGTCACCCa                          <  2:253906/135‑1 (MQ=255)                                                                                             gACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCGGCCACGCTGTCACCCAgc                        <  2:254283/136‑1 (MQ=255)                                                                                                  cGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCGGCCACGCTGTCCCCCAGCGCAc                    >  5:176145/1‑135 (MQ=255)                                                                                                     cACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCGGCCACGCTGTCACCCAGCGCACCttt                >  7:141844/1‑136 (MQ=255)                                                                                                     cACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCGGCCACGCTGTCACCCAGCGCACCttt                >  3:350592/1‑136 (MQ=255)                                                                                                                     gCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCGGCCACGCTGTCACCCAGCGCACCTTTCAGTCGCTCGACCa  <  8:20524/134‑1 (MQ=255)                                                                                                                     gCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCGGCCACGCTGTCACCCAGCGCACCTTTCAGTCGCTCGACCa  <  5:178902/134‑1 (MQ=255)                                                                                                                     gCTTCCAGGATCTGGCGCA‑‑TGCAGGCCCAGGTCCTGCTCGACAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCGGCCACGCTGTCACCCAGCGCAGCTTTCAGTCGCTCGACCa  <  1:313961/134‑1 (MQ=255)                                                                                                                                        ||                                                                                                                    TGTGCGACAGCTCGGCGAAACGGTCTTCGCTCTGCTCGTGATCCAGCTTCTCGATCAGCGGGTGGCTCGGGTTGAACTCGAAGATCGGCTTGGACTCCGGCACTTTCTGCCCGCTGGCTTCCAGGATCTGGCGCATCTGCAGGCCCAGGTCCTGCTCGCCAATGGCCAGGATCGCCGGCGAGTCGGTCAGGCGGTGCGAGACGCGCACTTCGGCCACGCTGTCACCCAGCGCACCTTTCAGTCGCTCGACCA  >  ACB143/4713359‑4713610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑