Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB143 2,020,873 (C)7→8 coding (606/1005 nt) tagO → putative undecaprenyl‑phosphateN‑acetylglucosaminyl 1‑phosphate transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,020,8661.C91.4% 202.2 / 12.0 58coding (599/1005 nt)tagOputative undecaprenyl‑phosphateN‑acetylglucosaminyl 1‑phosphate transferase
Reads supporting (aligned to +/- strand):  ref base . (4/1);  new base C (24/29);  total (28/30)
Fisher's exact test for biased strand distribution p-value = 1.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

TGGCATCGCCAGTATCGAGGCCATCTGCGTGTGCATGGGCGGAAGCCTGCTCTACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCAGGCTGGGTAAACCCGCTATTATTCTGGGGGTGGTTAATACTTTCG  >  ACB143/2020732‑2020990
                                                                                                                                        |                                                                                                                              
tGGCATCGCGAGTATCGAGGCGATCTGCGTTTGCATGGGCGGATGCCTGCTCTACTGGCTCACTGGTCATGCGGAGGCAGGGTGGCTTCCGATATTACTGGCGGCCAATGTGGCGGGCTTCCTGTGCTGGAATTTc                                                                                                                               <  8:118191/136‑1 (MQ=255)
       gCCAGTATCGAGGCCATCTGCGTGTGCATGGGCGGAAGCCTGCTCTACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTcccccccc                                                                                                                      >  8:316417/1‑135 (MQ=255)
       gCCAGTATCGAGGCCATCTGCGTGTGCATGGGCGGAAGCCTGCTCTACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTcccccccc                                                                                                                      >  6:334640/1‑135 (MQ=255)
       gCCAGTATCGAGGCCATCTGCGTGTGCATGGGCGGAAGCCTGCTCTACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTcccccccc                                                                                                                      >  5:73636/1‑135 (MQ=255)
       gCCAGTATCGAGGCCATCTGCGTGTGCATGGGCGGAAGCCTGCTCTACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTcccccccc                                                                                                                      <  1:340357/136‑2 (MQ=255)
       gCCAGTATCGAGGCCATCTGCGTGTGCATGGGCGGAAGCCTGCTCTACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTcccccccc                                                                                                                      >  5:219399/1‑135 (MQ=255)
       gCCAGTATCGAGGCCATCTGCGTGTGCATGGGCGGAAGCCTGCTCTACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTccccaccc                                                                                                                      >  6:101287/1‑135 (MQ=255)
       gCCAGTATCGAGGCCATCTGCGTGTGCATGGGCGGAAGCCTGCTCTACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCCTCCTGTGCTGGAATTTcccccccc                                                                                                                      >  6:325306/1‑135 (MQ=255)
                 aGGCCATCTGCGTGTGCATGGGCGGAAGCCTGCTCTACTGGCTCACTGGTCATGCCGCGGCAGCGTGGCTGCCGTTATTTCTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCACCCCCCGCACGTAttt                                                                                                             >  3:269598/1‑136 (MQ=255)
                    ccATCTGCGTGTGCATGGGCGGAAGCCTGCTCTACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCCCGTATTTTTa                                                                                                          >  8:78710/1‑136 (MQ=255)
                      aTCTGCGTGTGCATGGGCGGAAGCCTGCTCTACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATg                                                                                                        <  1:114430/136‑1 (MQ=255)
                      aTCTGCGTGTGCATGGGCGGAAGCCTGCTCTACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATg                                                                                                        >  6:184100/1‑136 (MQ=255)
                        cTGCGTGTGCATGGGCGGAAGCCTGCTCTACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATggg                                                                                                      >  4:296422/1‑136 (MQ=255)
                         tGCGTGTGCATGGGCGGAAGCCTGCTCTACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCGCACGTATTTTTATGGGc                                                                                                     >  3:329844/1‑135 (MQ=255)
                           cgggtgCATGGGCGGCAGCCGGCTCTACTGGCGAACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCTGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGc                                                                                                     <  8:252779/131‑1 (MQ=255)
                              gtgCATGGGCGGAAGCCTGCTCTACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGc                                                                                                >  8:152021/1‑136 (MQ=255)
                                 cATGGGCGGAAGCCTGCTCTACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCgg                                                                                             <  5:179792/136‑1 (MQ=255)
                                 cATGGGCGGAAGCCTGCTCTACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCg                                                                                              <  7:85923/135‑1 (MQ=255)
                                   tgtgCGGAAGCCTGCTCTACTTGCTCACTGGTCATGCCGAGTCAGCGTGGCTTCCGATATTACTGTCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCa                                                                                           <  8:172106/133‑1 (MQ=255)
                                   tGGGCGGAAGCCTGCTCTACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCa                                                                                           <  7:269626/136‑1 (MQ=255)
                                    gggCGGAAGCCTGCTCTACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAg                                                                                          >  3:291726/1‑136 (MQ=255)
                                        ggAAGCCTGCTCTACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGc                                                                                      >  1:243282/1‑136 (MQ=255)
                                            gCCTGCTCTACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTc                                                                                  >  3:331170/1‑136 (MQ=255)
                                                gCTCTACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTggg                                                                              <  1:123964/136‑1 (MQ=255)
                                                 ctctACTGGCTCCCTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTgg                                                                               <  4:217068/134‑1 (MQ=255)
                                                 ctctACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTggg                                                                              <  4:67978/135‑1 (MQ=255)
                                                 ctctACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTgg                                                                               <  7:325146/134‑1 (MQ=255)
                                                 ctctACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTgg                                                                               <  4:4324/134‑1 (MQ=255)
                                                   ctACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTAt                                                                           >  7:42107/1‑136 (MQ=255)
                                                   ctACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCAGATATTACTGGAGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATTGGCGATGCAGGAAGTGGCTGTCTGGGTAt                                                                           >  7:36982/1‑136 (MQ=255)
                                                    tACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTAATTTTTTGGGCGATGCCGGCAGTGGCTTTCTGGGTATc                                                                          >  3:310711/1‑136 (MQ=255)
                                                             aCTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAggg                                                                 <  5:84931/136‑1 (MQ=255)
                                                             aCTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAggg                                                                 <  3:329340/136‑1 (MQ=255)
                                                              cTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGgc                                                                >  4:324280/1‑136 (MQ=255)
                                                              cTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGgc                                                                >  5:105411/1‑136 (MQ=255)
                                                                 gTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGgctgc                                                             >  4:282994/1‑136 (MQ=255)
                                                                 gTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGgct                                                               <  6:300354/134‑1 (MQ=255)
                                                                 gTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAAGTTCCCCACCCGCACGTAATTTTATGGGCGATGCCGGCAGTTGCTTGCTGGGTATCGTGCTAGGgctgc                                                             >  4:25235/1‑136 (MQ=255)
                                                                      gCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCa                                                        >  2:122860/1‑136 (MQ=255)
                                                                      gCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTAATTTTATGGGCGATGCCGGAAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCa                                                        >  4:117481/1‑136 (MQ=255)
                                                                       ccGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCAt                                                       >  8:2888/1‑136 (MQ=255)
                                                                       ccGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCAt                                                       >  3:127110/1‑136 (MQ=255)
                                                                          aGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATCTCCCCCCCCGCACGTATTTTTCTg                                                                                                        <  8:326953/84‑1 (MQ=255)
                                                                          aGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATCTCCCCCCCCGCACGTATTTTTATg                                                                                                        >  7:326953/1‑84 (MQ=255)
                                                                          aGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAg                                                    <  5:234880/136‑1 (MQ=255)
                                                                              aGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGc                                                <  1:187355/136‑1 (MQ=255)
                                                                              aGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGc                                                <  8:131493/136‑1 (MQ=255)
                                                                                 gTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCAgg                                             <  7:98355/136‑1 (MQ=255)
                                                                                    gCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCAGGCTg                                          >  3:153077/1‑136 (MQ=255)
                                                                                         cGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCAGGCTGGGTaa                                     <  5:22818/136‑1 (MQ=255)
                                                                                         cGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCAGGCTGGGTaa                                     <  5:145363/136‑1 (MQ=255)
                                                                                            tattcttGGCGGCCACTGTGCCGGGCTTCGTTTGATGTAATTTCCCCCCCCGCACGTATTTTTATTGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCAGGCTGGGTAAAc                                   <  3:200602/129‑1 (MQ=255)
                                                                                            tatTACTGGCGGCCACTGTGGCGGGCCTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCAGGCTGGGTAAAcc                                  <  5:325306/136‑1 (MQ=255)
                                                                                                       gcCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCAGGCTGGGTAAACCCGCTATTATTc                       >  7:313767/1‑136 (MQ=255)
                                                                                                         cACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCAGGCTGGGTAAACCCGCTATTATTCTg                     <  3:296422/136‑1 (MQ=255)
                                                                                                         cACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCAGGCTGGGTAAACCCGCTATTATTCTg                     <  6:173274/136‑1 (MQ=255)
                                                                                                         cACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCAGGCTGGGTAAACCCGCTATTATTCTg                     <  1:283078/136‑1 (MQ=255)
                                                                                                          aCTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCAGGCTGGGTAAACCCGCTATTATTCTgg                    >  1:316774/1‑136 (MQ=255)
                                                                                                              tgGCGGTCTTCCTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCAGGCTGGGTAAACCCGCTATTATTCTGGGggt                <  6:177916/136‑1 (MQ=255)
                                                                                                                        ccTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCAGGCTGGGTAAACCCGCTATTATTCTGGGGGTGGTTAATAc       <  1:251707/135‑1 (MQ=255)
                                                                                                                        ccTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCAGGCTGGGTAAACCCGCTATTATTCTGGGGGTGGTTAATACt      >  3:375073/1‑136 (MQ=255)
                                                                                                                         cTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCAGGCTGGGTAAACCCGCTATTATTCTGGGGGTGGTTAATAc       <  7:286470/134‑1 (MQ=255)
                                                                                                                         cTGTGCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCAGGCTGGGTAAACCCGCTATTATTCTGGGGGTGGTTAATAc       >  8:286470/1‑134 (MQ=255)
                                                                                                                           gtgCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCAGGCTGGGTAAACCCGCTATTATTCTGGGGGTGGTTAATACTTTc   >  7:96817/1‑136 (MQ=255)
                                                                                                                           gtgCTGGAATTTCCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCAGGCTGGGTAAACCCGCTATTATTCTGGGGGTGGTTAATACTTTc   >  3:330140/1‑136 (MQ=255)
                                                                                                                            tgCTGGAATTTCCCCCCCCGCAGGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCAGGCTGGGTAAACCCGCTATTATTCTGGGGGTGGTTAATACTTTCg  <  3:137351/136‑1 (MQ=255)
                                                                                                                                        |                                                                                                                              
TGGCATCGCCAGTATCGAGGCCATCTGCGTGTGCATGGGCGGAAGCCTGCTCTACTGGCTCACTGGTCATGCCGAGGCAGCGTGGCTGCCGATATTACTGGCGGCCACTGTGGCGGGCTTCCTGTGCTGGAATTTCCCCCCCGCACGTATTTTTATGGGCGATGCCGGCAGTGGCTTTCTGGGTATCGTGCTAGGGCTGCTGGCATTAGTGGCAGGCTGGGTAAACCCGCTATTATTCTGGGGGTGGTTAATACTTTCG  >  ACB143/2020732‑2020990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: