Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,978,6120AC53.1% ‑2.2 / 29.3 52Q688P (CAG→CCG) sasA_19Adaptive‑response sensory‑kinase SasA
Reads supporting (aligned to +/- strand):  ref base A (10/13);  new base C (24/2);  total (34/18)
Fisher's exact test for biased strand distribution p-value = 3.96e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GGCGCTGGCTTCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCC‑TGGCCATGCGCCA‑GGCTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCAT  >  ACB143/5978479‑5978740
                                                                                                                                      |                                                                                                                                 
ggCGCTGGCTTCCTGGAACCGGGCCAGAAGCTCTTGGGCTTCACTCATGACCTGGTGATGGAGGCCGCTTATGCACTCAGGCCCGTGCACCAGCGCGCCTCTCTGCATGCACGTATCGCCC‑TGGCCAGGCGCCA‑g                                                                                                                                 <  6:83276/135‑1 (MQ=255)
     tGGCTTCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCC‑AGGCCCAGCGCCC‑GGCATgg                                                                                                                           >  6:125529/1‑136 (MQ=255)
     tGGCTTCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCC‑AGGCCAAGCGCCC‑CGCtttg                                                                                                                           >  6:132012/1‑134 (MQ=255)
     tGGCTTCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCCCCC‑AGGCCAGGCCCCC‑GGCGTgg                                                                                                                           >  5:323525/1‑136 (MQ=255)
         ttCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCC‑TGGCCCTGCGCCA‑CGCTTGGCCAg                                                                                                                       >  8:121583/1‑136 (MQ=255)
          tCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCC‑GGGCCATGCGCCA‑GGCGTGGCCAgg                                                                                                                      >  5:355724/1‑136 (MQ=255)
           ccTGCACCCGGGCCCGACCGGCTTGGGCTTCACTCATGACCGGGTGAGGGCGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCAGGCACGTATCGCCC‑TGGCCATGCGCCA‑GGCTGGGCCAgg                                                                                                                      <  1:211507/135‑1 (MQ=255)
               cAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCC‑AGGCCAAGCGCCC‑CGCTTGGCCAGGGCgca                                                                                                                 >  6:190759/1‑134 (MQ=255)
                 aCCTGGCCATAACGGCTTGGGCTTCACTATTGACCGGTTGAGGGAGGCCGGTTATGCACTCCCGCCCGTGCACCTGCGGGCCTCGCTGAATGCACTTATCGCCC‑TGGCCATGCGCCT‑GGCTTGGCCTGGGCGAACg                                                                                                               <  6:124273/136‑1 (MQ=255)
                  ccGGGCCATACCGGCTTGGGCTTCACTCATGCCGGGGTGAGGGAGGCCGCTTATGCACTCCCGCCCGTGCACCAGCGCGCCGCGCTGCATGCACGTATCGCCC‑TGGCCATGCGCCA‑GGCTTGGCCAGGGCGAACGc                                                                                                              <  3:157051/136‑1 (MQ=255)
                        cAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCC‑AGGCCATTCGCCA‑GGCTTTGGCCGGGGGAGCGCACGAgg                                                                                                        >  4:111670/1‑136 (MQ=255)
                                   gggCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCATGGCCTTACGCCC‑GGCTGTGCCAGGCGAAACCCACGAGG‑gttgttgcag                                                                                             >  2:305574/1‑132 (MQ=255)
                                   gggCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCC‑GTGCCAAGCGCCA‑CGCGTGGCCACGGCGAACCCCCGAGGTGTTGGTCGAg                                                                                             >  6:88620/1‑136 (MQ=255)
                                         cACTCATGACCGGGTGATGGCGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCC‑TGGCCATGCGCCA‑GGCTGGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCgcc                                                                                       <  5:190759/136‑1 (MQ=255)
                                            tCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCC‑TGGCCCATCCCCC‑CGCATGGCCATGGCGCACGCAAGACGTGTTGTTAGAGCTAgccacc                                                                                    >  7:56893/1‑134 (MQ=255)
                                            tCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCC‑AGGCCATGCGCCA‑CGCTTGGCCCCGGCGAACGCACGAGGTGTTGTTCGCGCTCgcccgc                                                                                    >  6:70805/1‑136 (MQ=255)
                                            tCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCC‑AGGCCAATCGCCA‑GGCTTGGGCAGGGCGAACGCACGAGGTGTTGTTAGAGCTCgcccgc                                                                                    >  8:258601/1‑136 (MQ=255)
                                                 aCCGGTTGATGGAGGCCCGTTATGCACTCACGCCGGGGCCCCAGCGCGCCTCGCTGAATGCACTTATCGCCC‑TGGCCATGCGCCT‑GGGTTTTCCTGGTTTAAAGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCt                                                                               <  2:304073/136‑1 (MQ=255)
                                                 aCCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCC‑TGGGCAAGCGCCACGGGTTGG‑CAGGGCGCACCCACCCGGTGGTGTTGGCGCTAGCccgcccgc                                                                                >  5:173577/1‑135 (MQ=255)
                                                  ccGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCC‑CGGCCAAGCGGCC‑GGCATGGCCAGGGCGCACGCACCAGGTGTTGTTTGAGCTCGCccgccagcga                                                                              >  2:56455/1‑134 (MQ=255)
                                                  ccGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCC‑AGGCCCAGCGCCC‑AGCGTTGGCACGGCGCACCCCCCCGGTGGTGTTGGAGGGAGCCACCCAGCTa                                                                              >  4:225860/1‑136 (MQ=255)
                                                  ccGCGTGAGGGAGCCCGCTAATGCCCCCACGCCCGTGCACCCGCGCGCCTCGCTGCATCCCCGTATCGCCC‑TGGCCATGCGCCA‑GGCTTGGCCCGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTa                                                                              <  7:240720/136‑1 (MQ=255)
                                                   cGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCC‑AGGCCATGCGCCC‑CGCTTGGCCAGGGCGCACGCCCGAGGTGTTGTTCGAGCTcgcccgcccccta                                                                              >  5:295429/1‑130 (MQ=255)
                                                        gATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCC‑GGGGCCTGCGCCC‑GGCGTGGGCAGGGCGCACGCACGAGGGGTTGTTGGAGCGCGCCCGGCCGCTACCAcgc                                                                        >  4:97894/1‑136 (MQ=255)
                                                        gATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCC‑GGGCCCTGCGCCC‑GGCTTGGCCAGGGCGAACGCCCGAGGTGTTGTTCGCGCTCGCCCGCCAGCTACACcgc                                                                        >  2:43018/1‑136 (MQ=255)
                                                        gATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCC‑GGGCCCATCCCCC‑CGCGTTGCCAGGGCGCACGCCCCCGGTGTTGTTAGAGCTAGCCCGCCAGCAACacagc                                                                        >  7:214709/1‑132 (MQ=255)
                                                         aTGGTGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCAGTTATCGCCC‑TGGCCATGCGCCA‑GGCTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAAcgcg                                                                       <  5:125283/136‑1 (MQ=255)
                                                         aTGGAGGCCGCTTTTGCACTCACGCCCGTGCACCAGCGCGCCGCGCTGCAGGCACTTAGCGCCC‑TGGCCAGGCGCCC‑GGCTGGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAAcgc                                                                        <  4:171609/135‑1 (MQ=255)
                                                         aTGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGGCC‑AGGCCACGCGCCC‑CGCATGGCGCGGGCGGACGCACCAGGGGGTGTTCGGGCGCGCCAGCCACCGACAAcccg                                                                       >  3:24490/1‑136 (MQ=255)
                                                         aTGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCC‑AGGCCAAGCGCCC‑CGCGTTGGCAGGGCGCACGCCCGAGGTGTTGTTAGAGCTAGCCAGCCCCCGACAAcccc                                                                       >  3:225740/1‑133 (MQ=255)
                                                          tGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCC‑TGGCCATGCGCCA‑GGCTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAAcgcg                                                                       <  3:222928/135‑1 (MQ=255)
                                                          tGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCC‑TGGCCATGCGCCA‑GGCTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAAcgcg                                                                       <  5:173277/135‑1 (MQ=255)
                                                             aGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCC‑AGGGCATGCGCCA‑GGCTTGGGCCCGGCGCACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCGACAACGCGcccg                                                                   >  1:204321/1‑134 (MQ=255)
                                                                ccGCTTATCCACTCCCGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCC‑TGGCCATCCGCAA‑GGCTTGGCCAGGGCGAACGCACGAGGGGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGccc                                                                <  5:206435/136‑1 (MQ=255)
                                                                  gCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCC‑TGGCCATGCGCCA‑GGCTTGGCCAGGGCGAAAGCCAGAGGTGTTGTTCGAGCTGGCCAGCCAGGTACAACGCGCCAGccccg                                                              >  5:51776/1‑135 (MQ=255)
                                                                  gCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCC‑TGGCCAAGCGCCC‑GGCTTGGCCAGGGGGAAAGCCCGAGGTGTTTGTGGAGCTCGCCCCCCAGCTACAACCCGCCAGccccg                                                              >  6:119360/1‑135 (MQ=255)
                                                                   cTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCC‑TGGGCCTGCGCCC‑GGCGTGGGCAGGGGGGACGCCCGAGGGGGTGTTCGGGCTCGCCCGCCCGCTACAACGCGCCCGccccgg                                                             >  3:250408/1‑134 (MQ=255)
                                                                   cTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCC‑TGGGCATGCGCCC‑CGGTTGGGCAGGGCGCACCCCCCCGGTGGTGTTGGCTCGCGCCAGCCCGCGACAACGCGcccccccccg                                                             >  1:322608/1‑134 (MQ=255)
                                                                         cACTCCCGCCCGTGCACCCGCGCGCCTCGCGGCATGCACGTAGCGCCC‑GGCCCAGGCGCCC‑GGCTTGGCCCGGGGGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGt                                                        <  5:22119/135‑1 (MQ=255)
                                                                         cACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCC‑AGGCCCAGCGCCC‑GCCGTGGCCAGGGGGGCAGCCCCCGGGGGTGTTGGGGCTCGCCCGCCAGCTACAAACCGCCCGCCCCgggggggt                                                       >  4:340854/1‑134 (MQ=255)
                                                                         cACTCACGCCCGTGCACCAGCGCGCCGCGCTGCATGCACGTAGCGCCC‑GGGCCATGCGCCA‑GGCTGGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGt                                                        <  6:169385/135‑1 (MQ=255)
                                                                                 cccGTGAACCAGCGCGCCTCGCTGCATGACCTTATCGCCC‑TGGCCATGCGCCT‑GGGTGGGCCTGGGGGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCgg                                               <  8:186273/136‑1 (MQ=255)
                                                                                        aCCAGCGCGCCTCGCTGCATGCACGTATCGGCC‑GGGCCATGCGCCC‑CGGGTTGCCCGGGCGCACGCCCGAGGTGTTGGTGGCGGTCGCCAGCCCGCAACAACGCCCCCGCCCCAGCGGGGTTGGAGCGGATGGCTg                                        >  8:231024/1‑136 (MQ=255)
                                                                                        aCCAGCGCGCCTCGCTGCATGCACGTATCGCCC‑AGGCCATGCGCCC‑GGCTTGGGCCGGGCGGCCGCACGAGGGGGTGTTGGCGCTCGGCCGCCCGCTACAACGCGCCAGCCCCGGGGGGGTTGGCGCGGATGAcgg                                        >  2:305711/1‑134 (MQ=255)
                                                                                             cgcgcCTCGCTGCATGCAGGTATGGCCC‑TGGCCATGCGCCA‑GGCTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAg                                   <  5:132012/136‑1 (MQ=255)
                                                                                               cgcCTCGCTGCATGCACGTATCGGCC‑CGGCCCAGCGCCC‑CGCGTGGGCCGGGCGGACGCCCGAGGGGTTGTTGGGGCGCGGCCGCCCGCGGCCACGCGCCCCCCCCGGCGGGGTTGGGGCGGGTGACGGCGCAGCg                                 >  4:25458/1‑136 (MQ=255)
                                                                                                       tgcatgcaCGTATCGCCC‑GGGCCCTGCGCCC‑GGCGTGGGCAGGGCGCACGCCCGCGGTGGTGTTGGGGCTCGCCCGCCCGCGACAACGCGCCCGCCCCGGGGGGGTTGGAGCGGGTGTCTGCGAAGCGTTCCTg                           >  8:240808/1‑134 (MQ=255)
                                                                                                               cgaaTCGCCC‑TGGCCATGCGCCA‑GGCTGGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTAc                 <  2:131438/133‑1 (MQ=255)
                                                                                                               cGTATCGCCC‑TGGCCATGCGCCA‑GGCTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTAc                 <  4:203740/136‑1 (MQ=255)
                                                                                                                    cGCCC‑TGGCCATGCGCCA‑GGCTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCCGCCCCTGCGGGGTTGCAGCGGCTGACTGCGAAGCGTTCCTGCAGCTTTTAcgcg              >  2:251916/1‑134 (MQ=255)
                                                                                                                    cGCCC‑AGGCCAAGCGCCC‑CGCGTGGGCAGGGCGGACGCACGAGGTGTTGTTCGCGCTCGCCAGCCCGCGAAAACCCGCCCGCCCCTCCGGGTTTGCAGCGGATGACTGCGAAGCGTTGCTGCACCGTTTAagcgg             >  6:174188/1‑134 (MQ=255)
                                                                                                                               aTGCGCCC‑GGCTTGGCCCGGGCGAACGCCCGAGGGGTTGTTAGAGCTCGCCCGCCAGCGACAACGCGCCAGCCCCAGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGcatcct  >  2:281554/1‑134 (MQ=255)
                                                                                                                                      |                                                                                                                                 
GGCGCTGGCTTCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCC‑TGGCCATGCGCCA‑GGCTTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCAT  >  ACB143/5978479‑5978740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑