| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 1,433,079 | 0 | T | G | 54.5% | 1.7 / 11.7 | 12 | intergenic (‑356/‑303) | yveA/CJ019_01212 | Aspartate‑proton symporter/IS110 family transposase ISPpu9 |
| Reads supporting (aligned to +/- strand): ref base T (0/5); new base G (6/0); total (7/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.16e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCGGATAAGGGTTTGAGAGATGCAGTGCTAGTGCCGGCCCTTTCGCGGGTGAACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCCTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAGCCGCCTT > ACB143/1432947‑1433176 | ccGGATAAGGGTTTGAGAGATGCAGTGCTAGTGCCGGCCCTTTCGCGGGTGAACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCCTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCGGTc > 4:287973/1‑136 (MQ=255) tttGAGAGATGCAGTGCTAGTGCCGGCCCTTTCGCGGGTGAACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGTGGCCCTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAAc < 6:283740/136‑1 (MQ=255) gagagaTGCAGTGCTAGTGCCGGCCCTTTCGCGGGTGAACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCCTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCGGTCGCGCCGCaaaaaa > 1:307036/1‑132 (MQ=255) cAGTGCTAGTGCCGGCCCTTTCGCGGGTGAACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCCTGTAGGAGATCACCCAGCCCTTCCGGGGGGCGCAGGGGCGCGGCAAACAAGAgggggtc > 7:329034/1‑131 (MQ=37) cAGTGCTAGTGCCGGCCCTTTCGCGGGTGAACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCCTGTAGGAGATCACCCAGCCCTTCCGGGCTGGGGGGTGGGGCAGCCAACAAGAGgtggtc > 1:271042/1‑133 (MQ=37) tAGTGCCGGCCCTTTCGCGGGTGAACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCCTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCGGTCGGGCAGCAAACCTGAGGGGTTCggggt > 5:364364/1‑132 (MQ=25) cGGCGCAGAATTCGAAATCTGTGGGGGCCCTGTAGGAGATCACCCAGCCCTTCCGGGCGGCGCGGGCGCGCCGCAAACAAGAGGTGTACGGCGTGAGTTGCCCCCCGCCGGTGACAATTTTAAAAAAAAAAAGccg > 2:246935/1‑134 (MQ=12) gcgcAGAATTCGAAATCTGTGGGGGCCCTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGtt < 3:165676/136‑1 (MQ=255) gcgcAGAATTCGAAATCTGTGGGGGCCCTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCGGTGGCGGCGCAAACAATAGGTGTTAGGCGTGAGTTACGCCGCGCCCCGGACATTTTTAAAAAAAAAAAGCGGtt > 4:292295/1‑136 (MQ=12) aGAATGCGAAATCTGTGGGGGCCCTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGcc < 1:92241/136‑1 (MQ=255) ggCCCTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAGCCGCCtt < 3:292295/136‑1 (MQ=11) ggCCCTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGAAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAGCCGCCtt < 3:39990/136‑1 (MQ=11) | CCGGATAAGGGTTTGAGAGATGCAGTGCTAGTGCCGGCCCTTTCGCGGGTGAACCCGCTCCTACAGGTACGGCGCAGAATTCGAAATCTGTGGGGGCCCTGTAGGAGATCACCCAGCCCTTCCGGGCTGCGCTGTCGCGCAGCAAACATGAGGTGTACGGCTTCAGTTCCGCAGCGACGCTGACATTTTGAAAAAGAAAAGGCGGTTGGCCCGGTAGGGCCAGCCGCCTT > ACB143/1432947‑1433176 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |