Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1431,524,7320TG57.9% ‑2.0 / 22.6 38Q67P (CAG→CCG) CJ019_01439hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (5/11);  new base G (0/22);  total (5/33)
Fisher's exact test for biased strand distribution p-value = 8.70e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CATGGCAGTGCTCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAGGGTTATCGGTATCTTGTA  >  ACB143/1524604‑1524861
                                                                                                                                |                                                                                                                                 
gaTGGCAGTGCTCGGGGGCAGCCGGTTCCAACTGGCTTTCCAGCAGGCGCTTAAAGTGCTCCAGGCGCTGGGACTGGTGCTGATGCTGGCGCAGCGGTCTCACCTGGCTCTGCTGGGCGGAAGGTTGCTGGGCggg                                                                                                                            <  6:315351/135‑1 (MQ=255)
        tGCTCGCGGGCAGCCGGTTGAAACTGGCTTTCCAGCTGGCGCTGCAAGTGCTCCAGGGGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACGTGGCGCAGCAGGGCGGAAGGTGGGTGGGGGGGGTAGGGt                                                                                                                     <  4:229760/135‑1 (MQ=255)
         gCTCGCGGGAAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCGCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGTAAGGTGGCTGGGCGGGGGAGGGttt                                                                                                                   <  1:165344/136‑1 (MQ=255)
          cTCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTg                                                                                                                  >  8:61617/1‑136 (MQ=255)
           tCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTg                                                                                                                  >  4:26854/1‑135 (MQ=255)
           tCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTg                                                                                                                  >  5:128052/1‑135 (MQ=255)
             gcgGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTAg                                                                                                               >  6:302336/1‑136 (MQ=255)
               gggCAGCCGGTGCAAACGGGCTTGCCAGCCGGCGCGGAAAGTGCTCCCGGCGCGCGGCGGGGTTCAGCAGCCGGCGCAGCGGGCGCACCTGGCGCAGCAGGGGGTAAGGGGGCGGGGGGGGGGCGGGTTTGTAGGc                                                                                                             <  8:116131/136‑1 (MQ=255)
                    gCCGGTTGAACCTGGCTTTCCCGCAGGCGCTGCAAGTGCGCCCGGCGCGCGGCCGGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCGGGGGGGGGGAGGGTTTGTAGGCCATgg                                                                                                        <  8:69091/136‑1 (MQ=255)
                     ccGGTTGCAACTGGCTTTCCAGCAGGCGCTGAAAGGGCTCCCGGCGCGCGGCCGGGTTCAGCAGCAGGCGCCGCGGGCGCACCTGGCGCAGCCGGGCGGATGGTGGCGGGGCGGGGGAGGGTTTGTAGGCCATggg                                                                                                       <  8:20664/136‑1 (MQ=255)
                              atgggCTTTCCAGCAGGCGCTGCATGTGCTCCAGGCGCTCGGCCTGGTTCTGCCGCAGGCGCCGCGGGCGCACCTGGCGCTGCTGGGGGTATGGGGGCGGGGGGGGGGCGGGTTTGTAGGCCATGGGGGTACGCGt                                                                                              <  2:230642/133‑1 (MQ=255)
                                tGGCTTGCCCGCTGGCGCTGAATGTGCGCCCGGGGCTCGGCCTGGTGCCGCCGCTGGCGCTGCGGGCGCACCGGGCGCAGCAGGGCGTAAGGGGGCGGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGa                                                                                            <  3:338341/136‑1 (MQ=255)
                                  gCTTTCCCGCAGGCGCTGCAAGTGCTCCTGGGTCTCGGCCTGGTTCAGCCGCAGGCGCAGCGGGCGCACGTGGCGCAGAAGGGCGTAAGGGGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGa                                                                                            <  8:95375/134‑1 (MQ=255)
                                  gCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCGCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCGGGGCTGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGa                                                                                            <  1:29494/134‑1 (MQ=255)
                                  gCTTACCAGCCGGCGCTGCAAGTGGCCCAGGCGCGCGGCCTGGTTCAGCCGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCGGGGCGGGGGTGGGTTTGTAGGCCATGGGGGTACGCGTGa                                                                                            <  8:134452/134‑1 (MQ=255)
                                    tttCCCGCCGGCGCTGCAAGGGCGCCTGCCGCTCGGCCGGGTCCCGCAGCCGGCGCTGGGGGCGCACCGGGCGCAGCCGGGGGGAAGGGGGCGGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTg                                                                                        <  4:261517/136‑1 (MQ=255)
                                    tttCCAGCAGGCGCTGCAAGGGCTCCAGGGGGGCGGCGTGGTTCACCAGACGGCGCAGCGGGCGCACGTGGGGCAGCCGGGCGTAAGGGGGCGGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTg                                                                                        <  7:304192/136‑1 (MQ=255)
                                    ttaccAGCCGGCGCTGCATGTGCGCCCGGCGCGCGGCCGGGTGCAGCCGCGGGCGCCGGGGGCGCACCGGGCGCAGCCGGGCGTATGGGGGCGGGGGGGGGGCGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTg                                                                                        <  2:214469/133‑1 (MQ=255)
                                     tgccAGCAGGCGCTGAAATGGCTCCCGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCTGGGCGTAAGGTGGGGGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTg                                                                                        <  5:83292/133‑1 (MQ=255)
                                        ccgccggCGCGGCATGGGCGCCCGGCGGGCGGCCTGGTTCAGCTGCAGGCGCAGCGGGCGCACCGGGCGCAGCCGGGCGGAAGGGGGCGGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGt                                                                                    <  5:359395/131‑1 (MQ=255)
                                        cagctgGCGCTGCAAGTGCTCCAGGCGCGGGGCCTGGTTCCGCCGCTGGCGCAGCGGGCGCACCGGGCGCAGCCGGGCGTATGGGGGCGGGGGGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGt                                                                                    <  3:309363/136‑1 (MQ=255)
                                        cagcagGCGCTGAATGTGCTCCAGGCGCGGGGCCGGGTTCAGCAGCCGGCGCAGGGGGCGCACGTGGCGCAGCAGGGCTGATGGGGGCTGGGGGGGGGCGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGt                                                                                    <  8:6841/136‑1 (MQ=255)
                                          gcagGCGCTGCAAGGGCTCCTGGGGCGGGGCCTGGTTCAGCAGCTGGCGCCGGGGGCGCACGTGGCGCAGCTGGGGGGAAGGGGGGGGGGGGGGGGTGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTgg                                                                                  <  4:283254/136‑1 (MQ=255)
                                          gcagGCGCTGCAAGGGCGCCTGGCGCGGGGCCTGGTGCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGATGGTGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTgg                                                                                  <  7:242488/136‑1 (MQ=255)
                                          gcagGCGCTGCAAGGGCGCCCGGCGCTGGGCCTGGTTCAGCAGCAGGCGCAGGGGGCGCACCTGGCGCAGCAGGGCGTAAGGGGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTgg                                                                                  <  7:135396/136‑1 (MQ=255)
                                          gcagGCGCGGCAAGTGCTCCCGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCGGGCGCCGCAGGGCGGAAGGTGGCGGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTgg                                                                                  <  2:203594/136‑1 (MQ=255)
                                           cagGCGCGGCAAGGGCGCCCGGCGCGGGGCCGGGTTCAGCCGCAGGCGCAGCGGGCGCACCGGGCGCAGCTGGGCGGAAGGGGGCGGGGGGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTggg                                                                                 <  4:57266/136‑1 (MQ=255)
                                               cgcTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGca                                                                               >  5:206122/1‑134 (MQ=255)
                                                  tGCATGTGCTCCAGGCGGTCGGCGGGGTTCAGCAGCAGGCGCTGGGGGCGCACCTGGGGCAGCAGGGCGTAAGGGGGCGGGGCGGGGGCGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTc                                                                          <  5:72396/136‑1 (MQ=255)
                                                    cAAGTGCTCCCGGCGCGCGGCGGGGTTCAGCACCCGGCGCCGCGGGCGCACCGGGCGCAGCAGGGCGTAAGGGGGCTGGGGGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTa                                                                        <  6:268516/136‑1 (MQ=255)
                                                                        ccgggTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGTAAGGGGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGcc                                                    <  8:24555/133‑1 (MQ=255)
                                                                                            cgCGGGCGCCCCGGCCGCTGCTGGGGGGTTGGGGGCGGGGGGGGGGTGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAAcc                                <  6:253493/135‑1 (MQ=255)
                                                                                             gCGGGCGCACCTGGCGCAGCAGGGCGGAAGGGGGCGGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAAccc                               <  8:158818/136‑1 (MQ=255)
                                                                                                   gccccTGGCGCTGCTGGGCGTAAGGTGGCTGGGGGGGGGTGGGTTTGTAGGCCTTGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCt                         <  1:344470/133‑1 (MQ=38)
                                                                                                           cgcAGCCGGGCGTATGGGGGCTGGGGGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGGGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAGGGt                 <  8:4911/136‑1 (MQ=38)
                                                                                                             cagccgGGCGTAAGGTGGCGGGGGGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAGGGTTa               <  2:325812/136‑1 (MQ=255)
                                                                                                              cgcgggggggACGGGGGCGGGGGGGGGGTGGGGTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAGGGTTAt              <  3:26854/132‑1 (MQ=38)
                                                                                                                           gTGGCGGGGGGGGGGTGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAGGGTTATCGGTATCTTGTa  <  4:333431/135‑1 (MQ=255)
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CATGGCAGTGCTCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAGGGTTATCGGTATCTTGTA  >  ACB143/1524604‑1524861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑