Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,094,8080AC62.8% 6.8 / 11.8 47L186R (CTG→CGG) CJ019_01995hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (12/4);  new base C (13/14);  total (26/21)
Fisher's exact test for biased strand distribution p-value = 1.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCAAG  >  ACB143/2094688‑2094936
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aTGCGGTCCAGTCCCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCCGCTGCTCTTCATACACCTGCGGCGCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCgg                                                                                                                   <  8:49390/136‑1 (MQ=255)
   cGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGGCCGGCGCGCcgggccgggcg                                                                                                                >  1:352950/1‑135 (MQ=255)
     gTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGGCAGGCCCGCCGGGCcgggcggg                                                                                                              >  8:43323/1‑136 (MQ=255)
         aGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCACGGCCGCCTGGCCGGGCAGGcgtg                                                                                                          >  4:261618/1‑133 (MQ=255)
                        gCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCTGGCCGGGGTGGGCGTTTTTCAGGGCCGGtt                                                                                           >  8:325828/1‑136 (MQ=255)
                         cccGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTg                                                                                          >  4:245376/1‑136 (MQ=255)
                          ccGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTAGGCTACGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCTGGCCGGGCGGGCCGTTTTGGCGGGCCGGGTgg                                                                                         >  4:7350/1‑136 (MQ=255)
                           cGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCGGGCCGGGGGGGGCGTTTTGGAGGGGCtggtggg                                                                                        >  7:70804/1‑135 (MQ=255)
                                cAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCGGGCCGGGCGGGCCGTTTTTCCGGGCCGGTTGGGCGAgg                                                                                   >  6:211894/1‑136 (MQ=255)
                                     gTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCTGGCCGGGCTTGCCGTTTTTCAGGGCCTGGTGGCCGAGgtggtt                                                                              >  4:129217/1‑136 (MQ=255)
                                      tttCTAAATATACGTCAGCTGCTATACAACCACATTCGGCGCGGGTGGCTGGCTCAAATCCTGCACCCCGCGCTCTAAGGCCCGGCTCGCCTGCCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGgttgtt                                                                              <  8:195162/135‑1 (MQ=255)
                                        actcAAGATAGGTCAGCTGCTATTCAACCCCCTTCGGCTCCGGCGGTTTGCCCAAATCCTGCACGCCGCGCGCCAAGGCCCGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTga                                                                            <  5:161604/134‑1 (MQ=255)
                                          ccacgATCCGGCAGCGGCGCTGCCTCCACCTGCGGCTCCGGCTGCGTGGGAAAATACTGCCCGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTgaaga                                                                         <  2:279988/132‑1 (MQ=255)
                                           cAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCCCGCCGGGGCCGGCGGGCCGTTTTGCCGGGCCTGTTGGCCGCGGTGGGTgaagca                                                                        >  4:132009/1‑134 (MQ=255)
                                                 aCGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGCCGCGCCTGGCCGCGCTGGCCGCTTTGCACGGCCAGGTTGCCGAGGTTGTTTAAGAACTCgat                                                                  >  1:212744/1‑136 (MQ=255)
                                                    tccgcggctCATCATACACCTTCGGCTCCGGCTGCTGGCCAAAATACTCCCCGCCGGGCTCGACGGCCAGGCGCGCCGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATg                                                               <  5:156385/130‑1 (MQ=255)
                                                     cAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCTGGCCGGGCTGGCCATTTTGCAGGGCCGGTTGGCCGAGGGTGTTGAAGAACTCTATATg                                                               >  8:336396/1‑135 (MQ=255)
                                                       gctgctCTACATCCACCTTCGGCGCGGGCTGCTTGCGAAAATCCTCCCCGCCGCGCTCGACGGCCCGGCGCGCCGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATgcag                                                            <  1:44730/136‑1 (MQ=255)
                                                         ggctCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATgcagca                                                          <  1:308143/135‑1 (MQ=255)
                                                           ctctTCATACCCCTGCGGCGCCGGCTGCTTGCGAAAATACTGCACGCCGCGCGCGAAGGCCTGGCGCGCGTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATgcagcag                                                         <  6:183592/135‑1 (MQ=255)
                                                                   ccacCTGCGGCTCGGGCTGCTGCCGAAAATCGGGCACGCCGCGCGCGAAGGCCCGGCGCGCGGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGat                                                <  1:212339/135‑1 (MQ=255)
                                                                          cgcccccGGCGGCTTCCGAAAATACTCCACGCCGCGCGCTACGGCCTGGCGCGCCGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGc                                           <  2:130779/129‑1 (MQ=255)
                                                                          cGGCTCCGGCGGCTGGCGCAAATACTGCACGCCGCGCGCGAAGGCCCGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCg                                          <  7:254840/135‑1 (MQ=255)
                                                                           ggCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCTGGCCGGGCTGGCCGTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGa                                        >  3:187609/1‑136 (MQ=255)
                                                                           ggCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCCCCAGGATATGGCGGa                                        >  4:298911/1‑136 (MQ=255)
                                                                           ggCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCAGGCCGGGCTGGCCGTTTTGCAGGGCCTGGTGGCCGAGGGTGTTGAAGAACTCAATATGCAGCAGCCCCAGGATATGGGGGa                                        >  7:160167/1‑136 (MQ=255)
                                                                             cTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCACGCGCCCCTGGCCCGGCGGGGCTTTTTGCAGGGCCAGTTGGCCGGTGTTTGTGTAAAACACTATTATCAACCGCACCAGGATATGGCGGAta                                      >  6:35452/1‑135 (MQ=255)
                                                                              cccGGCGGCTGGCGAAAAACCTCCCCGCCGCGCTCGATGGCCCGGCGCGCCGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAttt                                     <  8:70804/135‑1 (MQ=255)
                                                                              accGGCTGCTTGCGCAAATACTCCCCGCCGCGCTCTAAGGCCCGGCGCGCCGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAttt                                     <  4:55853/135‑1 (MQ=255)
                                                                                    tgcttgctAAAATACTCCACGCCGCGCTCGACGGCCCGGCGCGCCGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGa                               <  1:84703/136‑1 (MQ=255)
                                                                                     gctTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCTCGGCTGGCCGGGCTGGGCGTTTTTCAGGGCGTGTTGGCCGAGGGTGTTGAAGAACTCTATTTTCAGCAGCACCAGGATATTGCGGAGTTACTTggc                              >  7:134712/1‑134 (MQ=255)
                                                                                         ccgcAAATCCTGCCCGCCGCGCTCCCAGGCCCGGCGCGCGTGGCCGTGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCaa                          <  1:362994/135‑1 (MQ=255)
                                                                                                      cccccgcGCCCGCCGGCCGGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGa             <  7:319421/133‑1 (MQ=255)
                                                                                                      aCGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCGTTTTGCAGGGCCGGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGa             >  8:36435/1‑136 (MQ=255)
                                                                                                       cgccgcGCTCGAAGGCCCGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGa             >  8:105328/1‑135 (MQ=255)
                                                                                                       cgccgcGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCGGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAAAGCTTGGCCTCgg             >  2:205689/1‑134 (MQ=255)
                                                                                                        gccgcGCTCGAAGGCCGGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGCCACAATGCTTGGCCTCGa             >  5:241131/1‑134 (MQ=255)
                                                                                                        gccgcGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACa           >  5:163959/1‑136 (MQ=255)
                                                                                                        gccgcGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCGTTTTTCAGGGCCTGGTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAAAGCTTGGGCTCGa             >  4:154729/1‑134 (MQ=255)
                                                                                                        gccgcGCTCGAAGGCCAGGCGCGCCTGGCCGGGCGGGCCGTTTTTCAGGGCCTGTTGGCCGCGGTTGTTGAAGAACTCTATATGCCGCCGCACCAGGATATGGCGGAGTTCCTTGACCCAATGCTTGGCCTCGACa           >  4:149039/1‑136 (MQ=255)
                                                                                                        gccgcGCTCGAAGGCCAGGCGCCCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATTCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACa           >  4:182278/1‑136 (MQ=255)
                                                                                                             gcTCGAAGGCCCGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCggg      >  7:235568/1‑136 (MQ=255)
                                                                                                                 gccggCCCGGCGCGCGGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaag  <  3:10928/133‑1 (MQ=255)
                                                                                                                 gacggCCCGGCGGGCGGGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaag  <  3:30662/133‑1 (MQ=255)
                                                                                                                 gacggCCCGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaag  <  3:129632/133‑1 (MQ=255)
                                                                                                                 gacggCCCGGCGCGCCGGCCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaag  <  7:49333/133‑1 (MQ=255)
                                                                                                                   aGGCCCGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaag  <  2:28748/134‑1 (MQ=255)
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ATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCAAG  >  ACB143/2094688‑2094936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑