Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,189,9110TC46.5% 22.5 / 186.9 128T46A (ACC→GCC) CJ019_02075hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (38/30);  new base C (24/35);  total (63/65)
Fisher's exact test for biased strand distribution p-value = 1.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 128 total aligned reads displayed.

GTATGATTCGTCAAGATTTTGAAGGATGTTAACCATTTGTGGCATGTATATCTCCTGCCATCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGTGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCCTGCTGCTGCTCTGCACGAAGTGTGGCAGGTTTTCTTTGCTCTTTTTTCGA  >  ACB143/2189777‑2190043
                                                                                                                                      |                                                                                                                                    
  aTGATTCGTCAAGATTTTGAAGGATGTTAACCATTTGTGGCATGTATATCTCCTGCCATCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGTGAt                                                                                                                                   >  4:328114/1‑136 (MQ=255)
       tCGTCAAGATTTTGAAGGATGTTAACCATTTGTGGCATGTATATCTCCTGCCATCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGCGAtgttg                                                                                                                               >  6:271566/1‑135 (MQ=255)
            aaGATTTTGAAGGATGTTAACCATTTGTGGCATGTATATCTCCTGCCATCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGTGATGTTGTCTATc                                                                                                                         >  4:26035/1‑136 (MQ=255)
            aaGATTTTGAAGGATGTTAACCATTTGTGGCATGTATATCTCCTGCCATCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGTGATGTTGTCTATc                                                                                                                         >  2:33305/1‑136 (MQ=255)
            aaGATTTTGAAGGATGTTAACCATTTGTGGCATGTATATCTCCTGCCATCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGCGATGTTGTCTATc                                                                                                                         >  1:72235/1‑136 (MQ=255)
             aGATTTTGAAGGATGTTAACCATTTGTGGCATGTATATCTCCTGCCATCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGTGATGTTGTCTATCg                                                                                                                        >  7:40117/1‑136 (MQ=255)
              gATTTTGGAGGATGTTAACCATTTGTGGCATGTATATCTCCTGCCATCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGAGATGTTGTCTATCGt                                                                                                                       >  1:56643/1‑136 (MQ=255)
              gATTTTGAAGGATGTTAACCATTTGTGGCATGTATATCTCCTGCCATCCCTCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGCGATGTTGTCTATCGt                                                                                                                       >  1:135067/1‑136 (MQ=255)
              gATTTTGAAGGATGTTAACCATTTGTGGCATGTATATCTCCTGCCATCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGTGATGTTGTCTATCGt                                                                                                                       >  4:202805/1‑136 (MQ=255)
              gATTTTGAAGGATGTTAACCATTTGTGGCATGTATATCTCCTGCCATCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGTGATGTTGTCTATCGt                                                                                                                       >  1:120995/1‑136 (MQ=255)
              gATTTTGAAGGATGTTAACCATTTGTGGCATGTATATCTCATGCCATCCCGCCATCAATGCCTCGGGCTTACTCCGCAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGTGATGTTGTCTATCGt                                                                                                                       >  3:190933/1‑136 (MQ=255)
               aTTTTGAAGGATGTTAACCATTTGTGGCATGTATATCTCCTGCCATCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGTGATGTTGTCTATCGtt                                                                                                                      <  6:108233/136‑1 (MQ=255)
                      aGGATGTTAACCATTTGTGGCATGTATATCTCCTGCCATCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGTGATGTTGTCTATCGTTGCGCttt                                                                                                               <  6:341756/136‑1 (MQ=255)
                      aGGATGTTAACCATTTGTGGCATGTATATCTCCTGCCATCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGTGATGTTGTCTATCGTTGCGCttt                                                                                                               >  2:235238/1‑136 (MQ=255)
                      aGGATGTTAACCATTTGTGGCATGTATATCTCCTGCCATCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGCGATGTTGTCTATCGTTGCGCttt                                                                                                               <  5:271566/136‑1 (MQ=255)
                      aGGATGTTAACCATTTGTGGCATGTATATCTCCTGCCATCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGCGATGTTGTCTATCGTTGCGCttt                                                                                                               <  2:320402/136‑1 (MQ=255)
                             tAACAATTTGTGGCATGTATATCTCCTGCCATCCCGCCATCAATGCATCGGGATTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGCGATGTTGTCTATCGTTGCGCTTTGGTAGcc                                                                                                        >  2:82360/1‑136 (MQ=255)
                              aaCCATTTGTGGCATGTATATCTCCTGCCATCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGCGATGTTGTCTATCGTTGCGCTTTGGTAGcc                                                                                                        <  1:263662/135‑1 (MQ=255)
                                      gtgGCATGTATATCTCCTGCCATCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGTGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGc                                                                                                >  2:173473/1‑135 (MQ=255)
                                       tgGCATGTATATCTCCTGCCATCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGTGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCt                                                                                               >  1:302470/1‑135 (MQ=255)
                                       tgGCATGTATATCTCCTGCCATCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGTGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCt                                                                                               <  8:336859/135‑1 (MQ=255)
                                       tgGCATGTATATCTCCTGCCATCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGCGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCt                                                                                               <  2:245383/135‑1 (MQ=255)
                                       tgGCATGTATATCTCCTGCCATCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGCGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCt                                                                                               <  4:82882/135‑1 (MQ=255)
                                       tgGCATGTATATCTCCTGCCATCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGCGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCt                                                                                               <  5:304426/135‑1 (MQ=255)
                                         gCATGTATATCTCCTGCCATCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGTGATGTTGGCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAAt                                                                                            <  7:137165/136‑1 (MQ=255)
                                           aTGTATATCTCCTGCCATCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGCGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCg                                                                                          <  8:174188/136‑1 (MQ=255)
                                             gTATATCTCCTGCCATCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGTg                                                                                                                                     >  1:159252/1‑91 (MQ=255)
                                                       tGCCATCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGCGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAAtagtag                                                                              >  4:7733/1‑136 (MQ=255)
                                                         ccATCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGTGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGca                                                                            >  4:41864/1‑135 (MQ=255)
                                                         ccATCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGCGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGcc                                                                            >  7:97847/1‑136 (MQ=255)
                                                         ccATCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGCGATGTTGTCGATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATAGTTTCAATAGTAGcc                                                                            >  1:171870/1‑136 (MQ=255)
                                                           aTCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGTGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGccc                                                                           >  7:165811/1‑135 (MQ=255)
                                                           aTCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGTGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGccc                                                                           >  7:32842/1‑135 (MQ=255)
                                                           aTCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGTGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGccc                                                                           >  7:11172/1‑135 (MQ=255)
                                                           aTCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGTGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGccc                                                                           >  1:374320/1‑135 (MQ=255)
                                                           aTCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGTGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGccc                                                                           >  7:321353/1‑135 (MQ=255)
                                                           aTCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGTGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCg                                                                          <  4:48170/136‑1 (MQ=255)
                                                           aTCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGTGATGTTGTCTATCGTTGCGCTGTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGccc                                                                           >  8:289489/1‑135 (MQ=255)
                                                           aTCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGCGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGccc                                                                           >  6:310825/1‑135 (MQ=255)
                                                           aTCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGCGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGccc                                                                           >  4:176993/1‑135 (MQ=255)
                                                           aTCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGCGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGccc                                                                           >  4:84316/1‑135 (MQ=255)
                                                           aTCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGCGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGccc                                                                           >  8:146987/1‑135 (MQ=255)
                                                           aTCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGCGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGccc                                                                           >  8:181480/1‑135 (MQ=255)
                                                           aTCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGCGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGccc                                                                           >  3:187007/1‑135 (MQ=255)
                                                           aTCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGCGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGccc                                                                           >  7:220772/1‑135 (MQ=255)
                                                            tCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGCGAGGTGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGa                                                                         <  4:65770/136‑1 (MQ=255)
                                                                          ccTCGGGCTTACTCCGTAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGTCGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCTTTTCAATAGTAGCCCGAAGCAGTTTTTccc                                                            <  4:337459/135‑1 (MQ=255)
                                                                          ccTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGCGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTccc                                                            <  7:260268/135‑1 (MQ=255)
                                                                           cTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGTGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCcgc                                                          >  7:345306/1‑136 (MQ=255)
                                                                             cGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGTGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGt                                                        >  2:42310/1‑136 (MQ=255)
                                                                              ggtcTTACTCCGGAAATTTAGATAATATGTAGCTCGGACTGTTCCTGCATGAGAGGCGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGtt                                                       <  4:60617/133‑1 (MQ=255)
                                                                                gCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGCGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTc                                                     <  3:285610/136‑1 (MQ=255)
                                                                                    aCTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGCGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCctg                                                  <  8:97847/135‑1 (MQ=255)
                                                                                       ccGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGTGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCctgctgc                                              >  1:84757/1‑136 (MQ=255)
                                                                                        cGGAAATGTAGATAAAATGTAGCTCGTCCTGTTCCTGCATGAGAGGTGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCctgctgct                                             <  7:221851/136‑1 (MQ=255)
                                                                                        cGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGCGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCctgctgct                                             <  3:142402/136‑1 (MQ=255)
                                                                                         ggAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGTGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCctgctgctg                                            <  8:211326/136‑1 (MQ=255)
                                                                                             aTGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGTGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCCTGCTGCTGctct                                        >  4:209157/1‑136 (MQ=255)
                                                                                             aTGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGTGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCCTGCTGCTGctct                                        >  7:12658/1‑136 (MQ=255)
                                                                                             aTGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGTGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCCTGCTGCTGctct                                        >  5:88701/1‑136 (MQ=255)
                                                                                             aTGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGCGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCCTGCTGCTGctct                                        <  4:197363/136‑1 (MQ=255)
                                                                                             aTGTAGATAAAATGTAGCTCGGCCTGATCCTGCATGAGAGGCGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCCTGCTGCTGctct                                        >  7:157209/1‑136 (MQ=255)
                                                                                                tAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGTGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCCTGCTGCTGCTCTGc                                      <  2:127946/135‑1 (MQ=255)
                                                                                                tAGATAAAATGTAGCTCGGACTGTTCCTGCATGAGAGGCGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCCTGCTGCTTCTCTGCa                                     <  2:345277/136‑1 (MQ=255)
                                                                                                   aTAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGTGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCCTGCTGCTGCTCTGCACg                                   >  4:225037/1‑135 (MQ=255)
                                                                                                   aTAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGCGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCCTGCTGCTGCTCTGCACg                                   >  8:355497/1‑135 (MQ=255)
                                                                                                       aaTGTAGCTCGGCCTGTTCCTGCATGAGAGGCGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCCTGCTGCTGCTCTGCACGAAgtg                              <  3:261557/136‑1 (MQ=255)
                                                                                                        aTGTAGCTCGGCCGGTTCCTGCATGAGAGGCGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTATCTAATCGTTTCAAAAGTAGCCCGAAGCAGTTTTTCCCGCCTTTCCTGCTGCTGCTCTGCACGAAgtgt                             >  1:221418/1‑136 (MQ=255)
                                                                                                               tCGGCCTGTTCCTGCATGAGAGGCGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCCTGCTGCTGCTCTGCACGAAGTGTGGCAGGt                      >  6:300686/1‑136 (MQ=255)
                                                                                                                cGGCCTGTTCCTGCATGAGAGGTGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCCTGCTGCTGCTCTGCACGAAGTGTGGCAGGtt                     >  3:263998/1‑136 (MQ=255)
                                                                                                                 ggCCTGTTCCTGCATGAGAGGTGATGTTGTCTATCGTTg                                                                                                                     >  5:42623/1‑39 (MQ=255)
                                                                                                                 ggCCTGTTCCTGCATGAGAGGTGATGTTGTCTATCGTTg                                                                                                                     <  6:42623/39‑1 (MQ=255)
                                                                                                                      gTTCCTGCATGAGAGGTGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCctg                                                  >  3:121345/1‑101 (MQ=255)
                                                                                                                      gTTCCTGCATGAGAGGTGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCctg                                                  <  4:121345/101‑1 (MQ=255)
                                                                                                                              aTGAGAGTTGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCCTGCTGCTGCTCTGCACGAAGTGTGGCAGGTTTTCTTTGCTCtttt       <  3:259207/136‑1 (MQ=255)
                                                                                                                              aTGAGAGGTGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCCTGCTGCTGCTCTGCACGAAGTGTGGCAGGTTTTCTTTGCTCtttt       <  5:260271/136‑1 (MQ=255)
                                                                                                                              aTGAGAGGTGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCCTGCTGCTGCTCTGCACGAAGTGTGGCAGGTTTTCTTTGCTCtttt       <  3:52294/136‑1 (MQ=255)
                                                                                                                              aTGAGAGGCGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCCTGCTGCTGCTCTGCACGAAGTGTGGCAGGTTTTCTTTGCTCtttt       <  8:85067/136‑1 (MQ=255)
                                                                                                                                gagaGGCGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCCTGCTGCTGCTCTGCACGAAGTGTGGCAGGTTTTCTTTGCTCtttttt     <  4:291487/136‑1 (MQ=255)
                                                                                                                                gagaGGCGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCCTGCTGCTGCTCTGCACGAAGTGTGGCAGGTTTTCTTTGCTCttttt      >  1:371653/1‑135 (MQ=255)
                                                                                                                                 cgagGCGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGAAGCCCGAAGCAGTTTTTCCCGCGTTTCCTGCTGCTGCTCTGCACGAAGTGTGGCAGGTTTTCTTTGCTCtttttt     <  4:347537/134‑1 (MQ=255)
                                                                                                                                 agagGTGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCCTGCTGCTGCTCTGCACGAAGTGTGGCAGGTTTTCTTTGCTCtttttt     <  8:32842/135‑1 (MQ=255)
                                                                                                                                 agagGTGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCCTGCTGCTGCTCTGCACGAAGTGTGGCAGGTTTTCTTTGCTCtttttt     <  6:357523/135‑1 (MQ=255)
                                                                                                                                 agagGTGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCCTGCTGCTGCTCTGCACGAAGTGTGGCAGGTTTTCTTTGCTCtttttt     <  1:50280/135‑1 (MQ=255)
                                                                                                                                 agagGTGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCCTGCTGCTGCTCTGCACGAAGTGTGGCAGGTTTTCTTTGCTCtttttt     <  1:44345/135‑1 (MQ=255)
                                                                                                                                 agagGTGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCACGAAGCAGTTTTTCCCGCGTTTCCTGCTGCTGCTCTGCACGAAGTGTGGCAGGTTTTCTTTGCTCtttttt     <  3:41864/135‑1 (MQ=255)
                                                                                                                                 agagGTGATGTTGTCTATCGTTGCGCGTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCCTGCTGCTGCTCTGCACGAAGTGTGGCAGGTTTTCTTTGCTCtttttt     <  3:369568/135‑1 (MQ=255)
                                                                                                                                 agagGCGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCCTGCTGCTGCTCTGCACGAAGTGTGGCAGGTTTTCTTTGCTCtttttt     <  2:255510/135‑1 (MQ=255)
                                                                                                                                 agagGCGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCCTGCTGCTGCTCTGCACGAAGTGTGGCAGGTTTTCTTTGCTCtttttt     <  3:57069/135‑1 (MQ=255)
                                                                                                                                 agagGCGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCCTGCTGCTGCTCTGCACGAAGTGTGGCAGGTTTTCTTTGCTCtttttt     <  3:367264/135‑1 (MQ=255)
                                                                                                                                 agagGCGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCCTGCTGCTGCTCTGCACGAAGTGTGGCAGGTTTTCTTTGCTCtttttt     <  8:291780/135‑1 (MQ=255)
                                                                                                                                 agagGCGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCCTGCTGCTGCTCTGCACGAAGTGTGGCAGGTTTTCTTTGCTCTTTTTTc    <  1:319924/136‑1 (MQ=255)
                                                                                                                                 agagGCGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTGTCCTGCTGCTGCTCTGCACGAAGTGTGGCAGGTTTTCTTTGCTCtttttt     <  2:105151/135‑1 (MQ=255)
                                                                                                                                  gagGTGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCCTGCTGCTGCTCTGCACGAAGTGTGGCAGGTTTTCTTTGCTCTTTTTTCg   <  3:209157/136‑1 (MQ=255)
                                                                                                                                  gagGTGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTNGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCCTGCTGCTGCTCTGCACGAAGTGTGGCAGGTTTTCTTTGCTCTTTTTTCg   <  4:128484/136‑1 (MQ=255)
                                                                                                                                  gagGCGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCCTGCTGCTGCTCTGCACGAAGTGTGGCAGGTTTTCTTTGCTCTTTTTTCg   <  5:310825/136‑1 (MQ=255)
                                                                                                                                  gagGCGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCCTGCTGCTGCTCTGCACGAAGTGTGGCAGGTTTTCTTTGCTCTTTTTTCg   <  7:181480/136‑1 (MQ=255)
                                                                                                                                  gagGCGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCCTGCTGCTGCTCTGCACGAAGTGTGGCAGGTTTTCTTTGCTCTTTTTTCg   <  8:64637/136‑1 (MQ=255)
                                                                                                                                    ggTGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCCTGCTGCTGCTCTGCACGAAGTGTGGCAGGTTTTCTTTGCTCTTTTTTCGa  >  2:158245/1‑135 (MQ=255)
                                                                                                                                    ggTGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCCTGCTGCTGCTCTGCACGAAGTGTGGCAGGTTTTCTTTGCTCTTTATTCGa  >  4:313097/1‑135 (MQ=255)
                                                                                                                                      |                                                                                                                                    
GTATGATTCGTCAAGATTTTGAAGGATGTTAACCATTTGTGGCATGTATATCTCCTGCCATCCCGCCATCAATGCCTCGGGCTTACTCCGGAAATGTAGATAAAATGTAGCTCGGCCTGTTCCTGCATGAGAGGTGATGTTGTCTATCGTTGCGCTTTGGTAGCCTTTGTAGCTAATCGTTTCAATAGTAGCCCGAAGCAGTTTTTCCCGCGTTTCCTGCTGCTGCTCTGCACGAAGTGTGGCAGGTTTTCTTTGCTCTTTTTTCGA  >  ACB143/2189777‑2190043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑