Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1433,440,3460AC45.7% 22.7 / 10.9 35A24A (GCA→GCC) CJ019_03266hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (8/11);  new base C (16/0);  total (24/11)
Fisher's exact test for biased strand distribution p-value = 1.92e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGC  >  ACB143/3440220‑3440471
                                                                                                                              |                                                                                                                             
gCAACGGTCTGGCGGCTCGCCAGGCCCTCTACACGCCTGCGCTCGCGGTGCTGCTGTGCGCGCGGGGGCCCCCCGCCGGGGCGCCGGGGCGCGGCGGCGGGCCGCCCGCGCGGGCGGCGCCCGGGCAGCCTGGgtg                                                                                                                      <  8:220117/136‑1 (MQ=255)
gCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCAGCCTGGgtg                                                                                                                      >  3:368715/1‑136 (MQ=255)
gCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCAGCCTGGgtg                                                                                                                      >  8:108816/1‑136 (MQ=255)
gCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCCGGCCGCCGGGgtg                                                                                                                      >  4:237919/1‑136 (MQ=255)
       tCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGGGTGGCAgg                                                                                                               >  8:101852/1‑136 (MQ=255)
        cgggaggcGCGCAAGGCCCTCTACACGCGCGCGCGCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCGGGGGCGCTGCTGCTGGCCGGCTGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGc                                                                                                              <  1:109158/134‑1 (MQ=255)
         gggaggaGCGCAAGGCCCTCTACCCGCGCGCGCGCGTGGTGCTGCAGGGCGCGCTGGGGCCACCCGCCGGGGCGCCGTGGCGCGGCGGCGGGCCGCCAGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGcc                                                                                                             <  8:43002/135‑1 (MQ=255)
            aggaTCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCAGGCGGCGCCCGGGCCGCCGGGGGGTGGGAGGCCCAc                                                                                                          >  7:303069/1‑136 (MQ=255)
                   cAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCCCTGGCGGCGCCCCGGCCGCCTCGGGGTTGGAGGGCACCGccct                                                                                                     >  2:365452/1‑132 (MQ=255)
                    aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCTGGGTGTGGGAGGCCACCGCCGATaa                                                                                                  >  1:271519/1‑136 (MQ=255)
                    aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCGGGGGGTGGGAGGCCCACCCCTATaa                                                                                                  >  1:273858/1‑136 (MQ=255)
                    aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCCGGCGGCGCCCCGGCCGCCTAGGTGTGGCAGGCCCACCCCCATaa                                                                                                  >  6:15393/1‑136 (MQ=255)
                                  gcgcgcgcTCGAGGGGCTGCAGGGCGCGCGTGGGCCACCCGCCGGGGCGCCGGGGCGCTGCTGCGGGCCGCCCGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTg                                                                                    <  8:48649/136‑1 (MQ=255)
                                        gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCCCCGGGGTGTGGCCGGCCCCCGCCGATAATGCGCCGCTCGCTGTTAAgg                                                                              >  2:43999/1‑136 (MQ=255)
                                        gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCTGGCGGCCCCCCGGCCGCCGGGGTGTGGCAGGCCACCGCCGATAAGTCGCAGCGCGCGGATCAgg                                                                              >  1:164783/1‑136 (MQ=255)
                                          tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCTGGGGGTGGCCGGCCCCCGCCTATAAGGCGCAGCGCGCGGATCAGGcc                                                                            >  3:298449/1‑136 (MQ=255)
                                          tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGGAGGCCAACGCCGAAAATGCGCAGCTCGCGGCTCAGGcc                                                                            >  3:283915/1‑136 (MQ=255)
                                          tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCAGCCGGGGTGTGGGAGGCCACCGCCGATAAGTCGCCGCTCGCTGATCAGGcc                                                                            >  7:130206/1‑136 (MQ=255)
                                          tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCTGGGTGTGGCAGGCCAACGCCGATAATGCGCAGCTCGCGGATCAGGcc                                                                            >  3:227235/1‑136 (MQ=255)
                                          tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCCCTGGCGGCGCCCGGGCCCCCGGGGGGTGGGAGGCCCCCGCCTATAAAGCGCCGCTCGCGGATCAGGcc                                                                            >  3:262133/1‑136 (MQ=255)
                                                             gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGTGTGGCAGGCCCCCGCCTATAAGGCGCCGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGt                                                         >  7:291367/1‑136 (MQ=255)
                                                             gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCCCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGTGTGGCAGGCCCCCGCCGATAAGGCGCCGCTCGCGGCTCAGGCCGATGACGTCGGCCAGCAGt                                                         >  3:31444/1‑136 (MQ=255)
                                                               ttGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGCGGCGCCCGGGCCGCCGGGGGGTGGCCGGCGCCCGCCGATAAGGCGCAGCGCGCGGATCTGGCCGAGGCCTTCGGGAGGCAGTTg                                                       >  5:26988/1‑136 (MQ=255)
                                                                    ccACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCGGGGTGGGGCAGGCCCACGCCGATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCg                                                    >  7:189168/1‑134 (MQ=255)
                                                                                ggcccGTGGCGGGGGTGCTCGCCGCCCGCGGGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc                                      <  8:265412/133‑1 (MQ=255)
                                                                                gcgcgGTGGCGCTGGTGGTCGCCGCCAGCGGGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc                                      <  4:177707/135‑1 (MQ=255)
                                                                                 cGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgcg                                     <  7:16844/136‑1 (MQ=255)
                                                                                       ggCGCTGCTGCTGGCCGACAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGt                               >  7:89984/1‑136 (MQ=255)
                                                                                                     ccgcccgcGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGaa                 <  6:121672/136‑1 (MQ=255)
                                                                                                      cgccCGCGCTGGCGGCGCCCGGGCACCCGGGGTGTGGCAGGCCAACGCCGATAATGCGCAGCTCGCTGATCAGGCCGATGACGTAGGGAAGCCGTTGGCGGCGAAGGATAGCGCGCACCGGCGGGAGCGGGAGAAg                >  2:84876/1‑136 (MQ=255)
                                                                                                       gccACCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACCCCTATAATGCGCAGCTCGCGGATCAGGCCGCTGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCCCGGTCTGGTGCGGGAGAAgg               >  4:160643/1‑136 (MQ=255)
                                                                                                                 ccgccgcCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTgcagcagc     <  8:316119/132‑1 (MQ=255)
                                                                                                                  cggcgCCCGGGCAGCCTGTGGGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTTGAGCGGGAGAAGGCTgcagcagca    <  6:111578/136‑1 (MQ=255)
                                                                                                                   cccgcCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGcagcagcag   <  2:164783/134‑1 (MQ=255)
                                                                                                                    gcgcCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCagcagcagc  >  5:185778/1‑136 (MQ=255)
                                                                                                                              |                                                                                                                             
GCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGC  >  ACB143/3440220‑3440471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑