Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB143	1,312,593	A→G	Q255R (CAG→CGG)	aaeA_2 →	p‑hydroxybenzoic acid efflux pump subunit AaeA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	1,312,593	0	A	G	100.0%	226.8 / NA	65	Q255R (CAG→CGG)	aaeA_2	p‑hydroxybenzoic acid efflux pump subunit AaeA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/25);  total (40/25)

CTGATGGGCACGGGTGAACCGATGCAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCAGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGACCGCGAGCCGCAGTGATGGTGCTGGCTTAACAGG  >  ACB143/1312460‑1312717                                                                                                                                      |                                                                                                                             cTGATGGGCACGGGTGAACCGATGCAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGgc                                                                                                                            >  5:160184/1‑136 (MQ=255) cTGATGGGCACGGGTGAACCGATGCAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGgc                                                                                                                            >  3:152185/1‑136 (MQ=255) cTGATGGGCACGGGTGAACCGATGCAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGgc                                                                                                                            >  8:184775/1‑136 (MQ=255) cTGATGGGCACGGGTGAACCGATGCAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGgc                                                                                                                            >  2:3755/1‑136 (MQ=255) cTGATGGGCACGGGTGAACCGATGCAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGgc                                                                                                                            >  3:324882/1‑136 (MQ=255) cTGATGGGCACGGGTGAACCGATGCAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTGCAACTGGGTGCGACTGGCCCGgc                                                                                                                            >  2:134958/1‑136 (MQ=255) cTGATGGGCACGGGTGAACCGATGCAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGACGACCTTCAACTGGGTGCGACTGGCCCGgc                                                                                                                            >  7:151487/1‑136 (MQ=255)   gATGGGCACGGGTGAACCGATGCAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGTCCCGgcg                                                                                                                           >  8:158435/1‑135 (MQ=255)   gATGGGCACGGGTGAACCGATGCAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGgcgc                                                                                                                          >  7:129666/1‑136 (MQ=255)   gATGGGCACGGGTGAACCGATGCAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGgcg                                                                                                                           >  7:124889/1‑135 (MQ=255)   gATGGGCACGGGTGAACCGATGCAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGACAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGgcg                                                                                                                           >  5:366457/1‑135 (MQ=255)    aTGGGCACGGGTGAACCGATGCAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGgcgc                                                                                                                          <  7:123731/135‑1 (MQ=255)        gCACGGGTGAACCGATGCAGTGGCACGTTGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATAcc                                                                                                                     <  3:216507/136‑1 (MQ=255)        gCACGGGTGAACCGATGCAGGGGCACGTGGAGAGTATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATAcc                                                                                                                     <  1:65163/136‑1 (MQ=255)         cACGGGTGAACCGATGCAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCg                                                                                                                    >  5:112130/1‑136 (MQ=255)                 aaCCGATGCAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGAt                                                                                                            >  8:60959/1‑136 (MQ=255)                   ccGATGCAGGGGCACGTTGAGAGGATCAGACGCGGCATCACCGACCGCAACAGCTACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAAGTGGGTTCGACTGGCCCGGAGATTACCGGTGAGGATTc                                                                                                          <  1:19209/136‑1 (MQ=255)                    cGATGCAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCg                                                                                                         >  5:359078/1‑136 (MQ=255)                         cAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGCCCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTgg                                                                                                    >  4:20687/1‑136 (MQ=255)                         cAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTgg                                                                                                    <  2:101358/136‑1 (MQ=255)                         cAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTgg                                                                                                    <  8:339662/136‑1 (MQ=255)                         cAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTg                                                                                                     >  1:105762/1‑135 (MQ=255)                         cAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTg                                                                                                     >  6:248982/1‑135 (MQ=255)                         cAGGGGCACGTGGAGAGAATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTTCCGGAGGTACAGCAGACCTTCATCTGTGTGCGTCTGGCCCGGCGCCTACCGGTGAGGATGCGACTg                                                                                                     >  5:226151/1‑135 (MQ=255)                          aGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGa                                                                                                   >  5:332778/1‑136 (MQ=255)                               cACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAgg                                                                                              >  6:325143/1‑136 (MQ=255)                                   tGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTTCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGcc                                                                                          <  5:267366/136‑1 (MQ=255)                                   tGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGcc                                                                                          >  4:39548/1‑136 (MQ=255)                                    ggAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGCCCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCg                                                                                         >  8:290487/1‑136 (MQ=255)                                    ggAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCGGGATCAGGTGCCg                                                                                         >  1:149518/1‑136 (MQ=255)                                       gagCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCGGGATCAGGTGCCGGaa                                                                                      <  7:184464/136‑1 (MQ=255)                                         gCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGAGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAgg                                                                                    <  6:50346/136‑1 (MQ=255)                                             cAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACAGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCt                                                                                 >  2:226936/1‑135 (MQ=255)                                               gCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGAc                                                                              >  3:165077/1‑136 (MQ=255)                                                     gCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAg                                                                        >  4:153793/1‑136 (MQ=255)                                                         cACCGACCGCAACAGCAACCCTGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCg                                                                    <  1:127151/136‑1 (MQ=255)                                                          accgaccgCAACAGCAACCCGGTCAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCg                                                                    <  4:110685/135‑1 (MQ=255)                                                          accgaccgCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCg                                                                    <  7:240974/135‑1 (MQ=255)                                                           ccgaccgCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCggg                                                                  >  4:47416/1‑136 (MQ=255)                                                                 gCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGAc                                                            <  6:168426/136‑1 (MQ=255)                                                                     cAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGTATCATGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGc                                                         >  4:204695/1‑135 (MQ=255)                                                                      aGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGa                                                       <  6:150582/136‑1 (MQ=255)                                                                      aGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGa                                                       <  6:7956/136‑1 (MQ=255)                                                                            ccGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGc                                                 <  7:60959/136‑1 (MQ=255)                                                                            ccGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGc                                                 <  8:266123/136‑1 (MQ=255)                                                                                   gCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCa                                          >  7:346108/1‑136 (MQ=255)                                                                                   gCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCa                                          >  1:111818/1‑136 (MQ=255)                                                                                        ttGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAgg                                     <  4:337971/136‑1 (MQ=255)                                                                                           ctgcCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGAcc                                  <  7:184775/136‑1 (MQ=255)                                                                                                ggAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGACCGCGAg                             <  5:248982/136‑1 (MQ=255)                                                                                                ggAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGACCGCGAg                             <  2:46324/136‑1 (MQ=255)                                                                                                ggAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGACCGCGAg                             <  3:83798/136‑1 (MQ=255)                                                                                                 gAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGAGACGCCTGGATCAGGTGCCGGAAGGGCTGACGTTGAGTGCGGCGATGACGGCGAGTGTGCAAGTGCAGGAGGACCGCGAGc                            >  4:28034/1‑136 (MQ=255)                                                                                                     tAGAGCCGAACTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGACCGCGAGCCGCa                        <  8:327451/136‑1 (MQ=255)                                                                                                       gagCCGACCTTCAACTGGGTGCGACTGTCCCGGCGGATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGACGGGGTGACCTTGAGTGGGGTGATGACGGAGAGTGTGCAAGTGCACGAGGATCGCTAGCCGCAg                       <  8:81851/135‑1 (MQ=255)                                                                                                           cGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGACCGCGAGCCGCAGTGATg                  >  1:184976/1‑136 (MQ=255)                                                                                                           cGACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGCGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGACCGCGAGCCGCAGTGATg                  >  2:2182/1‑136 (MQ=255)                                                                                                            gACCTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCTTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGACCGCGAGCCGCAGTGATgg                 <  4:26502/136‑1 (MQ=255)                                                                                                               cTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGACCGCGAGCCGCAGTGATGGTGc              >  3:197542/1‑136 (MQ=255)                                                                                                               cTTCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGACCGCGAGCCGCAGTGATGGTGc              >  4:354050/1‑136 (MQ=255)                                                                                                                ttCAACTGGGTGCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGACCGCGAGCCGCAGTGATGGTGCt             >  2:192396/1‑136 (MQ=255)                                                                                                                           gCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGACCGCGAGCCGCAGTGATGGTGCTGGCTTAACAgg  >  6:262917/1‑136 (MQ=255)                                                                                                                           gCGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGACCGCGAGCCGCAGTGATGGTGCTGGCTTAACAgg  >  7:10440/1‑136 (MQ=255)                                                                                                                            cGACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGACCGCGAGCCGCAGTGATGGTGCTGGCTTAACAgg  >  7:216332/1‑135 (MQ=255)                                                                                                                             gACTGGCCCGGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGACCGCGAGCCGCAGTGATGGTGCTGGCTTAACAgg  >  1:177086/1‑134 (MQ=255)                                                                                                                                      |                                                                                                                             CTGATGGGCACGGGTGAACCGATGCAGGGGCACGTGGAGAGCATCAGCCGCGGCATCACCGACCGCAACAGCAACCCGGACAGCCAGTTGCTGCCGGAGGTAGAGCCGACCTTCAACTGGGTGCGACTGGCCCAGCGCATACCGGTGAGGATTCGCCTGGATCAGGTGCCGGAAGGGCTGACCTTGAGTGCGGGGATGACGGCGAGTGTGCAAGTGCACGAGGACCGCGAGCCGCAGTGATGGTGCTGGCTTAACAGG  >  ACB143/1312460‑1312717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑