Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB143 1,346,999 A→G R477R (CGT→CGC)  CJ019_01261 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1431,346,9990AG98.2% 200.0 / NA 54R477R (CGT→CGC) CJ019_01261hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (21/32);  minor base T (1/0);  total (22/32)
Fisher's exact test for biased strand distribution p-value = 4.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGGCCTTGCCGGTCAGGTCGTGCAGGTCTGGCACTTCCGAAGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTACGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGCACATACAGCGCCTTTGCGCTTGCCACCCTGGTTGACCGCAACGGCGG  >  ACB143/1346871‑1347123
                                                                                                                                |                                                                                                                            
caGGCCTTGCCGGTCAGGTCGTGCAGGTCTGGCACTTCCGAAGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGAcggc                                                                                                                       <  3:259105/135‑1 (MQ=255)
      ttGCCGGTCAGGTCGTGCAGGTCTGGCACTTCCGAAGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTTCGACGGCGGtcat                                                                                                                 >  4:96563/1‑136 (MQ=255)
      gtGCCGGTCAGGTCGTGCAGGTCTGGCACTTCCGAAGGCGATAACAGTGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGtcat                                                                                                                 <  2:175585/135‑1 (MQ=255)
         ccGGTCAGTTCGTTCATGTCTGGCACTTCAGAAGGCGAGAACAGGGTCCACTTGCCGTCATCTAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCAc                                                                                                              <  1:258211/136‑1 (MQ=255)
         ccGGTCAGGTCGTGCAGGTCTGGCACTTCCGAAGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCAc                                                                                                              <  6:101337/136‑1 (MQ=255)
         ccGGTCAGGTCGTGCAGGTCTGGCACTTCCGAAGGCGAGAACAGGGGACACTTGCCGTCATCGAAGACACGCATCAGGAAAAGGTCAGGGATCCAGTTGGCGGTGTTCATTTCGTGGGTGCGACGGCGGTCATCAc                                                                                                              >  2:278081/1‑136 (MQ=255)
           ggTCAGGTCGTGCAGGTCTGGCACTTCCGAAGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTAATGAACAGGTGAGGGATCCAGTTGGCGGTGTTGATGACGTGGGTGCGACGGCGGTCATAACCg                                                                                                            >  5:269647/1‑136 (MQ=255)
               aGGTCGTGCAGGTCTGGCACTTCCGAAGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGgtg                                                                                                         <  3:334397/135‑1 (MQ=255)
                   cGTGCAGGTCTGGCACTTCCGAAGGCGAGAACAGGGTCCACTTTCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCtt                                                                                                    <  1:308859/136‑1 (MQ=255)
                              ggCACTTCCGAAGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGa                                                                                         >  8:345833/1‑136 (MQ=255)
                               gCACTTCCGAAGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGCCACGCTTCATGAACAGGTCAGGGCTCCAGTTGGCGGTGTTCAAGTCGTGGGTGCGACGGCGGTCATCACCGCTGTTCTTGCGCAGCTCGAt                                                                                        >  1:111724/1‑136 (MQ=255)
                                 aCTTCCGAAGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGa                                                                                      <  3:70410/136‑1 (MQ=255)
                                       gAAGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAAttctt                                                                                <  1:14060/136‑1 (MQ=255)
                                        aaGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAAttcttc                                                                               <  8:308455/136‑1 (MQ=255)
                                           gCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGAt                                                                            <  4:241611/136‑1 (MQ=255)
                                            cGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATg                                                                           >  7:34541/1‑136 (MQ=255)
                                                  cAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGATGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAgg                                                                     >  4:183511/1‑136 (MQ=255)
                                                   aGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGt                                                                    <  2:231237/136‑1 (MQ=255)
                                                   aGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGt                                                                    <  1:297467/136‑1 (MQ=255)
                                                               ccGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTcc                                                         <  7:156460/135‑1 (MQ=255)
                                                               ccGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTcc                                                         <  7:209180/135‑1 (MQ=255)
                                                               ccGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTcc                                                         <  1:51027/135‑1 (MQ=255)
                                                               ccGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTcc                                                         <  1:327134/135‑1 (MQ=255)
                                                               ccGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTcc                                                         <  3:188798/135‑1 (MQ=255)
                                                               ccGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTcc                                                         <  7:346067/135‑1 (MQ=255)
                                                               ccGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTcc                                                         <  3:296610/135‑1 (MQ=255)
                                                               cagTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGGCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTcc                                                         <  4:207031/133‑1 (MQ=255)
                                                                  tCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAgg                                                      >  3:55209/1‑135 (MQ=255)
                                                                  tCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAgg                                                      >  1:113884/1‑135 (MQ=255)
                                                                       gAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGc                                                 <  4:310383/135‑1 (MQ=255)
                                                                       gAAGACACGCTTCATGAACAGGGCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGc                                                 <  1:149115/135‑1 (MQ=255)
                                                                         aGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTAGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGcac                                               <  2:201663/135‑1 (MQ=255)
                                                                           acacGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGCACATa                                            <  4:113166/136‑1 (MQ=255)
                                                                                    aTGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGCACATACAGCGCCtt                                   <  2:165734/136‑1 (MQ=255)
                                                                                        aCAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGCACATACAGCGCCTTTgcg                               <  4:176737/136‑1 (MQ=255)
                                                                                          aGGTCAGGGATCCAGTTGGCGGGGTTCATGTCGTGGGGGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGCACATACAGCGCCTTTGCGCt                             <  6:75574/136‑1 (MQ=255)
                                                                                            gTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCATCGATGTCCCGGTGCCAGGTTTCCAGGTAGGCACATACAGCGCCATTGCGCttt                           >  3:332853/1‑135 (MQ=255)
                                                                                              cAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTcc                                                         >  2:53145/1‑104 (MQ=255)
                                                                                              cAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTcc                                                         <  1:53145/104‑1 (MQ=255)
                                                                                              cAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGCACATACAGCGCCTTTTCGCTTGc                          >  5:15010/1‑135 (MQ=255)
                                                                                              cAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGCACATACAGCGCCTTTGCGCTTGcc                         >  8:49782/1‑136 (MQ=255)
                                                                                              cAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGCACATACAGCGCCTTTGCGCTTGcc                         >  1:187436/1‑136 (MQ=255)
                                                                                                         ttGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAAtt                                                                                   <  2:96212/67‑1 (MQ=255)
                                                                                                         ttGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAAtt                                                                                   >  1:96212/1‑67 (MQ=255)
                                                                                                         ttGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGCACATACAGCGCCTTTGCGCTTGCCACCCTGGTTGa              >  2:72076/1‑136 (MQ=255)
                                                                                                         ttGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGCACATACAGCGCCTTTGCGCTTGCCACCCTGGTTGa              >  6:113245/1‑136 (MQ=255)
                                                                                                         ttGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGCACATACAGCGCCTTTGCGCTTGCCACCCTGGTTGa              >  6:356517/1‑136 (MQ=255)
                                                                                                         ttGGCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCATGCGCAGCTCGATGAATTCTTAGATTTCCAGGTGCAAGGTTTACAGGTAGGCACATACAGCGCCTTTTCGCTTGCCACCCTGGTTGa              >  5:11380/1‑136 (MQ=255)
                                                                                                           ggCGGTGTTCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGCACATACAGCGCCTTTGCGCTTGCCACCCTGGTTGAc             <  1:7396/135‑1 (MQ=255)
                                                                                                                  ttCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGCACATACAGCGCCTTTGCGCTTGCCACCCTGGTTGACCGCAAcgg     <  8:259027/136‑1 (MQ=255)
                                                                                                                   tCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTa                                                    >  1:237670/1‑88 (MQ=255)
                                                                                                                   tCATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTa                                                    <  2:237670/88‑1 (MQ=255)
                                                                                                                    cATGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGCACATACAGCGCCTTTGCGCTTGCCACCCTGGTTGACCGCAAcggcg   >  1:17344/1‑136 (MQ=255)
                                                                                                                     aTGTCGTGGGTGCGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGCACATACAGCGCCTTTGCGCTTGCCACCCTGGTTGACCGCAAcggcgg  >  5:310017/1‑136 (MQ=255)
                                                                                                                                |                                                                                                                            
AAGGCCTTGCCGGTCAGGTCGTGCAGGTCTGGCACTTCCGAAGGCGAGAACAGGGTCCACTTGCCGTCATCGAAGACACGCTTCATGAACAGGTCAGGGATCCAGTTGGCGGTGTTCATGTCGTGGGTACGACGGCGGTCATCACCGGTGTTCTTGCGCAGCTCGATGAATTCTTCGATGTCCAGGTGCCAGGTTTCCAGGTAGGCACATACAGCGCCTTTGCGCTTGCCACCCTGGTTGACCGCAACGGCGG  >  ACB143/1346871‑1347123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑