Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB143 2,092,484 A→G Y155H (TAC→CAC)  rcsC_3 ← Sensor histidine kinase RcsC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1432,092,4840AG98.6% 280.6 / ‑3.4 73Y155H (TAC→CAC) rcsC_3Sensor histidine kinase RcsC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (45/27);  minor base C (1/0);  total (46/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01

TGGTCAGCATCATGTAGAACAGCGCCAGCAGAAGGCCGGTGAGCACCAGGCTGCGGGCAAAACCATTGAGCAGGGTGATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTACAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAACTGGCGTTGCTCGCCGAACAGGAAGTCGCTCAGTGGCCGATAGCGGTCCTGCAGTTTTGGCCGCTCGACATC  >  ACB143/2092352‑2092610
                                                                                                                                    |                                                                                                                              
tGGTCAGCATCATGTAGAACAGCGCCAGCAGAAGGCCGGTGAGCACCAGGCTGCGGGCAAAACCATTGAGCAGGGTGATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCa                                                                                                                              <  4:376919/135‑1 (MQ=255)
tGGTCAGCATCATGTAGAACAGCGCCAGCAGAAGGCCGGTGAGCACCAGGCTGCGGGCAAAACCATTGAGCAGGGTGATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCa                                                                                                                              <  2:282675/135‑1 (MQ=255)
    ctgcttcatGTAGACCTGCGCCAGCAGAAGGCCGGTGAGCACCAGGCTGCGGGCAAAACCATTGAGCATGGTGATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTg                                                                                                                         <  2:320624/131‑1 (MQ=255)
     aGCATCATGTAGAACAGCGCCAGCAGAAGGCCGGTGAGCACCAGGCTGCGGGCAAAACCATTGAGCAGGGTGATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTg                                                                                                                         <  7:168866/135‑1 (MQ=255)
         tcatGTAGAACAGCGCCAGCAGAAGGCCGGTGAGCACCAGGCTGCGGGCAAAACCATTGAGCAGGGTGATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAg                                                                                                                    <  4:73489/136‑1 (MQ=255)
         tcatGTAGAACAGCGCCAGCAGAAGGCCGGTGAGCACCAGGCTGCGGGCAAAACCATTGAGCAGGGTGATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAg                                                                                                                    <  8:11182/136‑1 (MQ=255)
               aGAACAGCGCCAGCAGAAGGCCGGTGAGCACCAGGCTGCGGGCAAAACCATTGAGCAGGGTGATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTg                                                                                                              >  1:370724/1‑136 (MQ=255)
                    aGCGCCAGCAGAAGGCCGGTGAGCACCAGGCTGCGGGCAAAACCATTGAGCAGGGTGATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTg                                                                                                         >  3:284802/1‑136 (MQ=255)
                    aGCGCCAGCAGAAGGCCGGTGAGCACCAGGCTGCGGGCAAAACCATTGAGCAGGGTGATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTg                                                                                                         >  7:241198/1‑136 (MQ=255)
                    aGCGCCAGCAGAAGGCCGGTGAGCACCAGGCTGCGGGCAAAACCATTGAGCAGGGTGATGCCGGCACGCTCGAGAAAGCGGCTGCCGAAGGCGCAGGTGACGATGTCCAGGTGCAGGGAGCCCAGGTATAGCTCTg                                                                                                         >  2:112755/1‑136 (MQ=255)
                      cgcCAGCAGAAGGCCGGTGAGCACCAGGCTGCGGGCAAAACCATTGAGCAGGGTGATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTgg                                                                                                        >  2:76740/1‑135 (MQ=255)
                       gcCAGCAGAAGGCCGGTGAGCACCAGGCTGCGGGCAAAACCATTGAGCAGGGTGATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCa                                                                                                      <  4:282304/136‑1 (MQ=255)
                       gcCAGCAGAAGGCCGGTGAGCACCAGGCTGCGGGCAAAACCATTGAGCAGGGTGATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCa                                                                                                      <  6:321427/136‑1 (MQ=255)
                         cagcagAAGGCCGGTGAGCACCAGGCTGCGGGCAAAACCATTGAGCAGGGTGATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCAtg                                                                                                    >  5:232755/1‑136 (MQ=255)
                         cagcagAAGGCCGGTGAGCACCAGGCTGCGGGCAAAACCATTGAGCAGGGTGATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCAtg                                                                                                    >  1:127667/1‑136 (MQ=255)
                         cagcagAAGGCCGGTGAGCACCAGGCTGCGGGCAAAACCATTGAGCAGGGTGATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCAtg                                                                                                    >  3:343303/1‑136 (MQ=255)
                         cagcagAAGGCCGGTGAGCACCAGGCTGCGGGCAAAACCATTGAGCAGGGTGATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCAtg                                                                                                    >  8:139562/1‑136 (MQ=255)
                         cagcagAAGGCCGGTGAGCACCAGGCTGCGGGCAAAACCATTGAGCAGGGTGATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCAtg                                                                                                    >  1:296808/1‑136 (MQ=255)
                         cagcagAAGGCCGGTGAGCACCAGGCTGCGGGCAAAACCATTGAGCAGGGTGATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCAtg                                                                                                    >  5:19855/1‑136 (MQ=255)
                         cagcagAAGGCCGGTGAGCACCAGGCTGCGGGCAAAACCATTGAGCAGGGTGATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCAtg                                                                                                    >  8:253531/1‑136 (MQ=255)
                         cagcagAAGGCCGGTGAGCACCAGGCTGCGGGCAAAACCATTGAGCAGGGTGATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCAtc                                                                                                    >  8:326973/1‑135 (MQ=255)
                         cagcagAAGGCCGGTGAGCACCAGGCTGCGGGCAAAACCATTGAGCAGGGTGATGCCGGCACGGTCGAGAAAGCGGCTGCAGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCAtg                                                                                                    >  7:342493/1‑136 (MQ=255)
                           gcagAAGGCCGGTGAGCACCAGGCTGCGGGCAAAACCATTGAGCAGGTTGATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCAtgtg                                                                                                  <  7:341781/136‑1 (MQ=255)
                            cagAAGGCCGGTGAGCACCAGGCTGCGGGCAAAACCATTGAGCAGGGTGATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTgg                                                                                                 <  4:268089/136‑1 (MQ=255)
                            cagAAGGCCGGTGAGCACCAGGCTGCGGGCAAAACCATTGAGCAGGGTGATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTgg                                                                                                 <  4:18384/136‑1 (MQ=255)
                                      gTGAGCACCAGGCTGCGGGCAAAACCATTGAGCAGGGTGATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACa                                                                                       <  2:47304/136‑1 (MQ=255)
                                         aGCACCAGGCTGCGGGCAAAACCATTGAGCAGGGTGATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGgc                                                                                    >  3:265376/1‑136 (MQ=255)
                                              cAGGCTGCGGGCAAAACCATTGAGCAGGGTGATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGCGTCAGGTACAGGCGCTcc                                                                               >  3:338117/1‑136 (MQ=255)
                                                ggCTGCGGGCAAAACCATTGAGCAGGGTGATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCtt                                                                             <  6:313086/136‑1 (MQ=255)
                                                    gCGGGCAAAACCATTGAGCAGGGTGATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAAc                                                                         >  1:47785/1‑136 (MQ=255)
                                                        gCAAAACCATTGAGCAGGGTGATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAACTGGc                                                                     >  2:191462/1‑136 (MQ=255)
                                                        gCAAAACCATTGAGCAGGGTGATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAACTGGc                                                                     >  8:221252/1‑136 (MQ=255)
                                                        gCAAAACCATTGAGCAGGGTGATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTCCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAACTGGc                                                                     >  3:237769/1‑136 (MQ=255)
                                                               cATTGAGCAGGGTGATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAACTGGCGTTGCTc                                                              >  6:15648/1‑136 (MQ=255)
                                                               cATTGAGCAGGGTGATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAACTGGCGTTGCTc                                                              <  1:372460/136‑1 (MQ=255)
                                                                     gCAGGGTGATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAACTGGCGTTGCTCGCCGaa                                                        >  4:316242/1‑136 (MQ=255)
                                                                       aGGGTGATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAACTGGCGTTGCTCGCCGaa                                                        >  4:335582/1‑134 (MQ=255)
                                                                           tGATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAACTGGCGTTGCTCGCCGAACAgg                                                    >  3:208789/1‑134 (MQ=255)
                                                                            gATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGc                                                                                                       <  4:189112/82‑1 (MQ=255)
                                                                            gATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGc                                                                                                       >  3:189112/1‑82 (MQ=255)
                                                                            gATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAACTGGCGTTGCTCGCCGAACAGGAAg                                                 >  1:151516/1‑136 (MQ=255)
                                                                            gATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAACTGGCGTTGCTCGCCGAACAGGAAg                                                 >  1:220841/1‑136 (MQ=255)
                                                                                ccGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAACTGGCGTTGCTCGCCGAACAGGAAGTCGc                                             <  4:343303/136‑1 (MQ=255)
                                                                                ccGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAACTGGCGTTGCTCGCCGAACAGGAAGTCGc                                             <  4:363772/136‑1 (MQ=255)
                                                                                ccGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAACTGGCGTTGCTCGCCGAACAGGAAGTCGc                                             <  4:108498/136‑1 (MQ=255)
                                                                                ccGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAACTGGCGTTGCTCGCCGAACAGGAAGTCGc                                             <  5:313690/136‑1 (MQ=255)
                                                                                 cGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTg                                                                                                                         <  6:351901/59‑1 (MQ=255)
                                                                                 cGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTg                                                                                                                         >  5:351901/1‑59 (MQ=255)
                                                                                 cGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAACTGGCGTTGCTCGCCGAACAGGAAGTCGCt                                            >  5:37949/1‑136 (MQ=255)
                                                                                 cGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAACTGGCGTTGCTCGCCGAACAGGAAGTCGCt                                            >  4:205928/1‑136 (MQ=255)
                                                                                 cGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAACTGGCGTTGCTCGCCGAACAGGAAGTCGCt                                            >  5:103555/1‑136 (MQ=255)
                                                                                 cGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAACTGGCGTTGCTCGCCGAACAGGAAGTCGCt                                            >  2:168154/1‑136 (MQ=255)
                                                                                 cGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAACTGGCGTTGCTCGCCGAACAGGAAGTCGCt                                            <  3:316242/136‑1 (MQ=255)
                                                                                   gCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAACTGGCGTTGCTCGCCGAACAGGAAGTCGCt                                            >  1:285673/1‑134 (MQ=255)
                                                                                      cGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAACTGGCGTTGCTCGCCGAACAGGAAGTCGCTCAGTg                                       >  5:349044/1‑136 (MQ=255)
                                                                                      cGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAACTGGCGTTGCTCGCCGAACAGGAAGTCGCTCAGTg                                       >  1:220373/1‑136 (MQ=255)
                                                                                      cGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAACTGGCGTTGCTCGCCGAACAGGAAGTCGCTCAGTg                                       >  7:30763/1‑136 (MQ=255)
                                                                                      cGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAACTGGCGTTGCTCGCCGAACAGGAAGTCGCTCAGTg                                       >  7:104492/1‑136 (MQ=255)
                                                                                           gagaAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAACTGGCGTTGCTCGCCGAACAGGAAGTCGCTCAGTGGCCGa                                  <  5:129312/136‑1 (MQ=255)
                                                                                           gagaAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAACTGGCGTTGCTCGCCGAACAGGAAGTCGCTCAGTGGCCGa                                  >  5:329564/1‑136 (MQ=255)
                                                                                           gagaAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCAGGTGGGTCAGGTACAGGCGCTCCTTGAACTGGCGTTGCTCGCCGCACAGGAAGTCGCTCAGTGGCCGa                                  >  8:353915/1‑136 (MQ=255)
                                                                                            agaAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAACTGGCGTTGCTCGCCGAACAGGAAGTCGCTCAGTGGCCGa                                  >  6:356754/1‑135 (MQ=255)
                                                                                            agaAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAACTGGCGTTGCTCGCCGAACAGGAAGTCGCTCAGTGGCCGa                                  >  4:122647/1‑135 (MQ=255)
                                                                                                 gCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAACTGGCGTTGCTCGCCGAACAGGAAGTCGCTCAGTGGCCGATAGCgg                            >  1:285707/1‑136 (MQ=255)
                                                                                                      tGCCGAAGGCGAAGGTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAACTGGCGTTGCTCGCCGAACAGGAAGTCGCTCAGTGGCCGATAGCGGTCCTg                       >  1:94889/1‑136 (MQ=255)
                                                                                                          gaaggcgaaggTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAACTGGCGTTGCTCGCCGAACAGGAAGTCGCTCAGTGGCCGATAGCGGTCCTGCAGt                   >  6:204530/1‑136 (MQ=255)
                                                                                                                 aaggTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAACTGGCGTTGCTCGCCGAACAGGAAGTCGCTCAGTGGCCGATAGCGGTCCTGCAGTTTTGGcc            <  2:29961/136‑1 (MQ=255)
                                                                                                                 aaggTGTCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAACTGGCGTTGCTCGCCGAACAGGAAGTCGCTCAGTGGCCGATAGCGGTCCTGCAGTTTTGGcc            <  2:247314/136‑1 (MQ=255)
                                                                                                                    gtgtCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAACTGGCGTTGCTCGCCGAACAGGAAGTCGCTCAGTGGCCGATAGCGGTCCTGCAGTTTTGGCCGCt         <  4:109192/136‑1 (MQ=255)
                                                                                                                      gtCGATGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAACTGGCGTTGCTCGCCGAACAGGAAGTCGCTCAGTGGCCGATAGCGGTCCTGCAGTTTTGGCCGCTCg       <  1:76740/136‑1 (MQ=255)
                                                                                                                          aTGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAACTGGCGTTGCTCGCCGAACAGGAAGTCGCTCAGTGGCCGATAGCGGTCCTGCAGTTTTGGCCGCTCGACAt   >  2:375551/1‑136 (MQ=255)
                                                                                                                          aTGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAACTGGCGTTGCTCGCCGAACAGGAAGTCGCTCAGTGGCCGATAGCGGTCCTGCAGTTTTGGCCGCTCGACAt   >  6:45782/1‑136 (MQ=255)
                                                                                                                           tGTCCAGGTGCAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAACTGGCGTTGCTCGCCGAACAGGAAGTCGCTCAGTGGCCGATAGCGGTCCTGCAGTTTTGGCCGCTCGACATc  <  2:220841/136‑1 (MQ=255)
                                                                                                                                    |                                                                                                                              
TGGTCAGCATCATGTAGAACAGCGCCAGCAGAAGGCCGGTGAGCACCAGGCTGCGGGCAAAACCATTGAGCAGGGTGATGCCGGCACGGTCGAGAAAGCGGCTGCCGAAGGCGAAGGTGTCGATGTCCAGGTACAGGGTGCCCAGGTATTGCTCTGGCATGTGGGTCAGGTACAGGCGCTCCTTGAACTGGCGTTGCTCGCCGAACAGGAAGTCGCTCAGTGGCCGATAGCGGTCCTGCAGTTTTGGCCGCTCGACATC  >  ACB143/2092352‑2092610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: