Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB143	2,281,980	A→G	K408R (AAG→AGG)	pepN →	Aminopeptidase N

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	2,281,980	0	A	G	100.0%	155.1 / NA	41	K408R (AAG→AGG)	pepN	Aminopeptidase N
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/8);  total (33/8)

GGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCAACTTCTACACCCTGACCGTCTACGAGAAGGGCGCCGAAGTGGTGCGCATGGTCCGTACCCTGTTGGGTAGCGAAGGCTTCCGTAAGGGCAGCGACCTGTACTTTGAACGTCATGATGGCCAGGCGGTAACCACCGACGACTTCATCAAGGCCATGGAAGACGCCAACGGCGTCGACTTTACCCAGTTCAAGCGCTGGTACAGCCAGGCGGGCAC  >  ACB143/2281847‑2282109                                                                                                                                      |                                                                                                                                  ggCCCCATGGCCCACCCGGTGCGCCCGGACATCTTCATCGAAATCTCCAACTTCTACACCCTGACCGTCTACGAGAAGGGCGCCGAAGTGGTGCGCATGGTCCGTACCCTGTTGGGTAGCGAAGGCTTCCGTAggg                                                                                                                                 <  1:293694/136‑1 (MQ=255) ggCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCAACTTCTACACCCTGACCGTCTACGAGAAGGGCGCCGAAGTGGTGCGCATGGTCCGTACCCTGTTGGGTAGCGAAGGCTTCCGTAggg                                                                                                                                 <  3:183657/136‑1 (MQ=255)    cccATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCAACTTCTACACCCTGACCGTCTACGAGAAGGGCGCCGAAGTGGTGCGCATGGTCCGTACCCTGTTGGGTAGCGAAGGCGTCCGTAgggg                                                                                                                                >  2:320805/1‑134 (MQ=255)      cATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCAACTTCTACACCCTGACCGTCTACGAGAAGGGCGCCGAAGTGGTGCGCATGGTCCGTACCCTGTTGGGTAGCGAAGGCTTCCGTAGGGGCAGc                                                                                                                            <  3:258735/136‑1 (MQ=255)                       gcCCGGACAGCTTCATCGAAATCTCCAACTTCTACACCCTGACCGTCTACGAGAAGGGCGCCGAAGTGGTGCGCATGGTCCGTACCCTGTTGGGTAGCGAAGGCTTCCGTAGGGGCAGCGACCTGTACTTTGAACg                                                                                                           >  2:220170/1‑136 (MQ=255)                          cGGACAGCTTCATCGAAATCTCCAACTTCTACACCCTGACCGTCTACGAGAAGGGCGCCGAAGTGGTGCGCATGGTCCGTACCCTGTTGGGTAGCGAAGGCTTCCGTAGGGGCAGCGACCTGTACTTTGAACGTCa                                                                                                        <  6:168552/136‑1 (MQ=255)                             aCAGCTTCATCGAAATCTCCAACTTCTACCCCCTGACCGTCTACGAGAAGGGCGCCGAAGTGGTGCGCATGGTCCGTACCCTGTTGGGTAGCGAAGGCTTCCGTAGGGGCAGCGACCTGTACTTTGAACGTCatga                                                                                                     >  6:220930/1‑136 (MQ=255)                               aGCTTCATCGAAATCTCCAACTTCTACACCCTGACCGTCTACGAGAAGGGCGCCGAAGTGGTGCGCATGGTCCGTACCCTGTTGGGTAGCGAAGGCTTCCGTAGGGGCAGCGACCTGTACTTTGAACGTCatgatg                                                                                                   <  5:113233/136‑1 (MQ=255)                                    cATCGAAATCTCCAACTTCTACACCCTGACCGTCTACGAGAAGGGCGCCGAAGTGGTGCGCATGGTCCGTACCCTGTTGGGTAGCGAAGGCTTCCGTAGGGGCAGCGACCTGTACTTTGAACGTCATGATGGCCAg                                                                                              >  4:95516/1‑136 (MQ=255)                                      tCGAAATCTCCAACTTCTACACCCTGACCGTCTACGAGAAGGGCGCCGAAGTGGTGCGCATGGTCCGTACCCTGTTGGGTAGCGAAGGCTTCCGTAGGGGCAGCGACCTGTACTTTGAACGTCATGATGGCCAGGc                                                                                            <  1:49970/136‑1 (MQ=255)                                           aTCTCCAACTTCTACACCCTGACCGTCTACGAGAAGGGCGCCGAAGTGGTGCGCATGGTCCGTACCCTGTTGGGTAGCGAAGGCTTCCGTAGGGGCAGCGACCTGTACTTTGAACGTCATGATGGCCAGGCGGTaa                                                                                       <  7:185701/136‑1 (MQ=255)                                                                 ccGTCTACGAGAAGGTCGCCGAAGTGGTGCGCATGGTCCGTACCCTGTAGGGTAGCGAAGGCTTCCGTAGGGGCAGCTACCTGTACATTGAACCTCAAGATGGCCAGGCGGTAAACACCTACGACTTAATCAAGGc                                                                 >  1:330820/1‑136 (MQ=255)                                                                 ccGTCTACGAGAAGGGCGCCGAAGTGGTGCGCATGGTCCGTACCCTGTTGGGTAGCGAAGGCTTCCGTAGGGGCAGCGACCTGTACTTTGAACGTCATGATGGCCAGGCGGTAACCACCGACGACTTCATCAAGGc                                                                 >  4:52465/1‑136 (MQ=255)                                                                   gTCTACGAGAAGGGCGCCGAAGTGGTGCGCATGGTCCGTACCCTGTTGGGTAGCGAAGGCTTCCGTAGGGGCAGCGACCTGTACTTTGAACGTCATGATGGCCAGGCGGTACCCACCGACGACTTCATCAAGGCCa                                                               >  7:71593/1‑136 (MQ=255)                                                                   gTCTACGAGAAGGGCGCCGAAGTGGTGCGCATGGTCCGTACCCTGTTGGGTAGCGAAGGCTTCCGTAGGGGCAGCGACCTGTACTTTGAACGTCATGATGGCCAGGCGGTAACCACCGACGACTTCATCAAGGCCa                                                               >  3:90037/1‑136 (MQ=255)                                                                   gTCTACGAGAAGGGCGCCGAAGTGGTGCGCATGGTCCGTACCCTGTTGGGTAGCGAAGGCTTCCGTAGGGGCAGCGACCTGTACTTTGAACGTCATGATGGCCAGGCGGTAACCACCGACGACTTCATCAAGGCCa                                                               >  5:162330/1‑136 (MQ=255)                                                                       aCGAGAAGGGCGCCGAAGTGGTGCGCATGGTCCGTACCCTGTTGGGTAGCGAAGGCTTCCGTAGGGGCAGCGACCTGTACTTTGAACGTCATGATGGCCAGGCGGTAACCACCGACGACTTCATCAAGGCCATgg                                                            >  4:1749/1‑135 (MQ=255)                                                                        cgtgaAGGGCGCCGAAGTGGTGCGCATGGTCCGTACCCTGTTGGGTAGCGAAGGCTTCCGTAGGGGCAGCGACCTGTACTTTGAACGTCATGATGGCCAGGCGGTAACCACCGACGACTTCATCAAGGCCATg                                                             <  7:182854/130‑1 (MQ=255)                                                                           gaAGGGCGCCGAAGTGGTGCGCATGGTCCGTACCCTGTTGGGTAGCGAAGGCTTCCGTAGGGGCAGCGACCTGTTCTTTGAACGTCATGATGGCCAGGCGGTAACCACCGACGACTTCATCAAGGCCATTGAAGAc                                                       >  2:103545/1‑136 (MQ=255)                                                                                 cgcCGAAGTGGTGCGCATGGTCCGTACCCTGTTGGGTAGCGAAGGCTTCCGTAGGGGCAGCGACCTGTACTTTGAACGTCATGATGGCCAGGCGGTAACCACCGACGACTTCATCAAGGCCATGGAAGACGCCAAc                                                 >  7:355177/1‑136 (MQ=255)                                                                                 cgcCGAAGTGGTGCGCATGGTCCGTACCCTGTTGGGTAGCGAAGGCTTCCGTAGGGGCAGCGACCTGTACTTTGAACGTCATGATGGCCAGGCGGTAACCACCGACGACTTCATCAAGGCCATGGAAGACGCCAAc                                                 >  7:350176/1‑136 (MQ=255)                                                                                   gcttaGTGGGGGGCTTGGTCCGTACCCTGTTGGTTAGGTTAGGCTTCCGTTGGGGCAGCGACCTGTACTTTGAACGTAATTATGGCCAGGCGGTAACCACCGACGACTTCATCAAGGCCATGGAAGACGCCAACgg                                               <  8:250111/132‑1 (MQ=255)                                                                                    cGAAGTGGTGCGCATGGTCCGTACCCTGTTGGGTAGCGAAGGCTTCCGTAGGGGCAGCGACCTGTACTTTGAACGTCATGATGGCCAGGCGGTAACCACCGACGACTTCATCAAGGCCATGGAAGACGCCAACGGc                                              >  6:216176/1‑136 (MQ=255)                                                                                              cgcATGGTCCGTACCCTGTTGGGTAGCGAAGGCTTCCGTAGGGGCAGCGACCTGTACTTTGAACGTCATGATGGCCAGGCGGTAACCACCGACGACTTCATCAAGGCCATGGAAGACGCCAACGGCGTCGACTTTa                                    >  3:18045/1‑136 (MQ=255)                                                                                                 aTGGTCCGTACCCTGTTGGGTAGCGAAGGCTTCCGTAGGGGCAGCGACCTGTACTTTGAACGTCATGATGGCCAGGCGGTAACCACCGACGACTTCATCAAGGCCATGGAAGACGCCAACGGCGTCGACTTTAcc                                  >  3:346994/1‑135 (MQ=255)                                                                                                 aTGGTCCGTACCCTGTTGGGTAGCGAAGGCTTCCGTAGGGGCAGCGACCTGTACTTTGAACGTCATGATGGCCAGGCGGTAACCACCGACGACTTCATCAAGGCCATGGAAGACGCCAACGGCGTCGACTTTAcc                                  >  2:218539/1‑135 (MQ=255)                                                                                                            ccTGTTGGGTAGCGAAGGCTTCCGTAGGGGCAGCGACCTGTACTTTGAACGTCATGATGGCCAGGCGGTAACCACCGACGACTTCATCAAGGCCATGGAAGACGCCAACGGCGTCGACTTTACCCAGTTCAAgcgc                      >  5:92486/1‑136 (MQ=255)                                                                                                            ccTGTTGGGTAGCGAAGGCTTCCGTAGGGGCAGCGACCTGTACTTTGAACGTCATGATGGCCAGGCGGTAACCACCGACGACTTCATCAAGGCCATGGAAGACGCCAACGGCGTCGACTTTACCCAGTTCAAgcgc                      >  6:165071/1‑136 (MQ=255)                                                                                                            ccTGTTGGGTAGCGAAGGCTTCCGTAGGGGCAGCGACCTGTACTTTGAACGTCATGATGGCCAGGCGGTAACCACCGACGACTTCATCAAGGCCATGGAAGACGCCAACGGCGTCGACTTTACCCAGTTCAAgcgc                      >  2:55432/1‑136 (MQ=255)                                                                                                              tGTTGGGTAGCTAAGGCTTCCGTAGGGGCAGCGACCTGTACTTTGAACGTCATGATGGCCAGGCGGTAACCACCGACGACTTCATCAAGGCCATGGAAGACGCCAACGGCGTCGACTTTACCCAGTTCAAGCGCTg                    <  7:246645/136‑1 (MQ=255)                                                                                                                 tGGGTAGCGAAGGCTTCCGTAGGGGCAGCGACCTGTACTTTGAACGTCATGATGGCCAGGCGGTAACCACCGACGACTTCATCAAGGCCATGGAAGACGCCAACGGCGTCGACTTTACCCAGTTCAAGCGCTGGTa                 >  5:53430/1‑136 (MQ=255)                                                                                                                  gggTAGCGAAGGCTTCCGTAGGGGCAGCGACCTGTACTTTGAACGTCATGATTGCCAGGCGGTTACCACCGACGACTTCATCAAGGCCATGGAAGACGCCAACGGCGTCGACTTTACCCAGTTCAAGCGCTGGTa                 >  5:242706/1‑135 (MQ=255)                                                                                                                  gggTAGCGAAGGCTTCCGTAGGGGCAGCGACCTGTACTTTGAACGTCATGATGGCCAGGCGGTAACCACCGACGACTTCATCAAGGCCATGGAAGACGCCAACGGCGTCGACTTTACCCAGTTCAATCGCTGGTAc                >  4:290513/1‑136 (MQ=255)                                                                                                                  gggTAGCGAAGGCTTCCGTAGGGGCAGCGACCTGTACTTTGAACGTCATGATGGCCAGGCGGTAACCACCGACGACTTCATCAAGGCCATGGAAGACGCCAACGGCGTCGACTTTACCCAGTTCAAGCGCTGGTa                 >  7:179931/1‑135 (MQ=255)                                                                                                                  gggTAGCGAAGGCTTCCGTAGGGGCAGCGACCTGTACTTTGAACGTCATGATGGCCAGGCGGTAACCACCGACGACTTCATCAAGGCCATGGAAGACGCCAACGGCGTCGACTTTACCCAGTTCAAGCGCTGGTa                 >  7:269450/1‑135 (MQ=255)                                                                                                                  gggTAGCGAAGGCTTCCGTAGGGGCAGCGACCTGTACTTTGAACGTCATGATGGCCAGGCGGTAACCACCGACGACTTCATCAAGGCCATGGAAGACGCCAACGGCGTCGACTTTACCCAGTTCAAGCGCTGGTa                 >  4:68214/1‑135 (MQ=255)                                                                                                                  gggTAGCGAAGGCTTCCGTAGGGGCAGCGACCTGTACTTTGAACGTCATGATGGCCAGGCGGTAACCACCGACGACTTCATCAAGGCCATGGAAGACGCCAACGGCGTCGACTTTACCCAGTTCAAGCGCTGGTa                 >  8:141897/1‑135 (MQ=255)                                                                                                                  gggTAGCGAAGGCTTCCGTAGGGGCAGCGACCTGTACTTTGAACGTCATGATGGCCAGGCGGTAACCACCGACGACTTCATCAAGGCCATGGAAGACGCCAACGGCGTCGACTTTACCCAGTTCAAGCGCTGGTa                 >  8:145136/1‑135 (MQ=255)                                                                                                                  gggTAGCGAAGGCTTCCGTAGGGGCAGCGACCTGTACTTTGAACGTCATGATGGCCAGGCGGTAACCACCGACGACTTCATCAAGGCCATGGAAGACGCCAACGGCGTCGACTTTACCCAGTTCAAGCGCTGGTa                 >  8:257098/1‑135 (MQ=255)                                                                                                                  gggTAGCGAAGGCTTCCGTAGGGGCAGCGACCTGTACTTTGAACGTCATGATGGCCAGGCGGTAACCACCGACGACTTCATCAAGGCCATGGAAGACGCCAACGGCGTCGACTTTACCCAGTTCAAGCGCTGGTa                 >  8:297405/1‑135 (MQ=255)                                                                                                                  gggTAGCGAAGGCTTCCGTAGGGGCAGCGACCTGTACTTTGAACGTCATGATGGCCAGGCGGTAACCACCGACGACTTCATCAAGGCCATGGAAGACGCCAACGGCGTCGACTTTACCCAGTTCAAGCGCTGGTa                 >  8:69785/1‑135 (MQ=255)                                                                                                                  gggTAGCGAAGGCTTCCGTAGGGGCAGCGACCTGTACTTTGAACGTCATGATGCCCAGGCGGTAACCACCGACGACTTCATCAAGGCCATGGAAGACGCCAACGGCGTCGACTTTACCCAGTTCAAGCGCTGGTa                 >  6:66653/1‑135 (MQ=255)                                                                                                                        cGAAGGCTTCCGTAGGGGCAGCGACCTGTACTTTGAACGTCATGATGGCCAGGCGGTAACCACCGACGACTTCATCAAGGCCATGGAAGACGCCAACGGCGTCGACTTTACCCAGTTCAAGCGCTGGTACAGCCAg          >  3:233686/1‑136 (MQ=255)                                                                                                                                tCCGTAGGGGCAGCGACCTGTACTTTGAACGTCATGATGGCCAGGCGGTAACCACCGACGACTTCATCAAGGCCATGGAAGACGCCAACGGAGTCGACTTTACCCAGTTCAAGCGCTGGTACAGCCAGGCGGGCAc  >  5:220754/1‑136 (MQ=255)                                                                                                                                      |                                                                                                                                  GGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCAACTTCTACACCCTGACCGTCTACGAGAAGGGCGCCGAAGTGGTGCGCATGGTCCGTACCCTGTTGGGTAGCGAAGGCTTCCGTAAGGGCAGCGACCTGTACTTTGAACGTCATGATGGCCAGGCGGTAACCACCGACGACTTCATCAAGGCCATGGAAGACGCCAACGGCGTCGACTTTACCCAGTTCAAGCGCTGGTACAGCCAGGCGGGCAC  >  ACB143/2281847‑2282109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑