Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB143	2,433,832	A→G	intergenic (+30/‑71)	CJ019_02296 → / → topA	hypothetical protein/DNA topoisomerase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	2,433,832	0	A	G	100.0%	79.5 / NA	26	intergenic (+30/‑71)	CJ019_02296/topA	hypothetical protein/DNA topoisomerase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/15);  total (11/15)

CGTCGAATTTGACGGCGAGCAGGTGCCACGCTTTGCTTTGGTCAAGGCCGAAGAGGGGCTGCCCGGGTTGCTGGGCAAGTCGCACGCGTGATCGTCCGAGCTTGACCTCACACTTTTGCCACTATATATAGCGGTGCCGCGTCTGGCACTTGGCGCGTTTTTTATCTTCAGATTCAGGAATACTCCGATCCATGGGCAAATCGCTGGTCATTGTGGAATCCCCGGCCAAGGCCAAGACCATC  >  ACB143/2433712‑2433953                                                                                                                         |                                                                                                                          cGTCGAATTTGACGGCGAGCAGGTGCCACGCTTTGCTTTGGTCAAGGCCGAAGAGGGGCTGCCCGGGTTGCTGGGCAAGTCGCACGCGTGATCGTCCGAGCTTGACCTCACACTTTTGCCGCTATATATAGCGGTg                                                                                                            >  7:234596/1‑136 (MQ=255)    cGAATTTGACGGCGAGCAGGTGCCACGCTTTGCTTTGGTCAAGGCCGAAGAGGGGCTGCCCGGGTTGCTGGGCAAGTCGCACGCGTTATCGTCCGAGCTTGACCTCACACTTTTGCCGCTATATATATCGGTGCcg                                                                                                         >  6:251906/1‑136 (MQ=255)    cGAATTTGACGGCGAGCAGGTGCCACGCTTTGCTTTGGTCAAGGCCGAAGAGGGGCTGCCCGGGTTGCTGGGCAAGTCGCACGCGTGATCGTCCGAGCTTGACCTCACACTTTTGCCGCTATATATAGCGGTGCcg                                                                                                         >  7:104892/1‑136 (MQ=255)    cGAATTTGACGGCGAGCAGGTGCCACGCTTTGCTTTGGTCAAGGCCGAAGAGGGGCTGCCCGGGTTGCTGGGCAAGTCGCACGCGTGATCGTCCGAGCTTGACCTCACACTTTTGCCGCTATATATAGCGGTGCcg                                                                                                         >  6:123815/1‑136 (MQ=255)    cGAATTTGACGGCGAGCAGGTGCCACGCTTTGCTTTGGTCAAGGCCGAAGAGGGGCTGCCCGGGTTGCTGGGCAAGTCGCACGCGTGATCGTCCGAGCTTGACCTCACACTTTTGCCGCTATATATAGCGGTGCcg                                                                                                         >  3:264899/1‑136 (MQ=255)    cGAATTTGACGGCGAGCAGGTGCCACGCTTTGCTTTGGTCAAGGCCGAAGAGGGGCTGCCCGGGTTGCTGGGCAAGTCGCACGCGTGATCGTCCGAGCTTGACCTCACACTTTTGCCGCTATATATAGCGGTGCcg                                                                                                         >  3:365760/1‑136 (MQ=255)    cGAATTTGACGGCGAGCAGGTGCCACGCTTTGCTTTGGTCAAGGCCGAAGAGGGGCTGCCCGGGTTGCTGGGCAAGTCGCACGCGTGATCGTCCGAGCTTGACCTCACACTTTTGCCGCTATATATAGCGGTGCcg                                                                                                         >  8:240384/1‑136 (MQ=255)                                    ctttgGTCAAGGCCGAAGAGGGGCTGCCCGGGTTGCTGGGCAAGTCGCACGCGTGATCGTCCGAGCTTGACCTCACACTTTTGCCGCTATATATAGCGGTGCCGCGTCTGGCACTTGGCGCGTTTTTTATCTTCAg                                                                         <  7:240384/136‑1 (MQ=255)                                    ctttgGTCAAGGCCGAAGAGGGGCTGCCCGGGTTGCTGGGCAAGTCGCACGCGTGATCGTCCGAGCTTGACCTCACACTTTTGCCGCTATATATAGCGGTGCCGCGTCTGGCACTTGGCGCGTTTTTTATCTTCAg                                                                         <  7:305035/136‑1 (MQ=255)                                    ctttgGTCAAGGCCGAAGAGGGGCTGCCCGGGTTGCTGGGCAAGTCGCACGCGTGATCGTCCGAGCTTGACCTCACACTTTTGCCGCTATATATAGCGGTGCCGCGTCTGGCACTTGGCGCGTTTTTTATCTTCAg                                                                         <  5:123815/136‑1 (MQ=255)                                    ctttgGTCAAGGCCGAAGAGGGGCTGCCCGGGTTGCTGGGCAAGTCGCACGCGTGATCGTCCGAGCTTGACCTCACACTTTTGCCGCTATATATAGCGGTGCCGCGTCTGGCACTTGGCGCGTTTTTTATCTTCAg                                                                         <  3:175433/136‑1 (MQ=255)                                      ttgGTCAAGGCCGAAGAGGGGCTGCCCGGGTTGCTGGGCAAGTCGCACGCGTGATCGTCCGAGCTTGACCTCACACTTTTGCCGCTATATATAGCGGTGCCGCGTCTGGCACTTGGCGCGTTTTTTATCTTCAGAt                                                                       >  6:13609/1‑136 (MQ=255)                                          tCAAGGCCGAAGAGGGGCTGCCCGGGTTGCTGGGCAAGTCGCACGCGTGATCGTCCGAGCTTGACCTCACACTTTTGCCGCTATATATAGCGGTGCCGCGTCTGGCACTTGGCGCGTTTTTTATCTTCAGATTCAg                                                                   <  3:284301/136‑1 (MQ=255)                                               gCCGAAGAGGGGCTGCCCGGGTTGCTGGGCAAGTCGCACGCGTGATCGTCCGAGCTTGACCTCACACTTTTGCCGCTATATATAGCGGTGCCGCGTCTGGCACTTGGCGCGTTTTTTATCTTCAGATTCAGGAATa                                                              <  3:372263/136‑1 (MQ=255)                                               gCCGAAGAGGGGCTGCCCGGGTTGCTGGGCAAGTCGCACGCGTGATCGTCCGAGCTTGACCTCACACTTTTGCCGCTATATATAGCGGTGCCGCGTCTGGCACTTGGCGCGTTTTTTATCTTCAGATTCAGGAATa                                                              <  3:138134/136‑1 (MQ=255)                                                     gagGGGCTGCCCGGGTTGCGGGGAAAGTCGCACGCGTGATCGTCCGAGCTTGACCTCACACTTTTGACGCTATATATAGCGGTGCCGCGTCTGGCACTTGGCGCGTTTTTTATCTTCAGATTCAGGAATACTCCGa                                                        <  8:258779/136‑1 (MQ=255)                                                              cccGGGTTGCTGGGCAAGTCGCACGCGTGATCGTCCGAGCTTGACCTCACACTTTTGCCGCTATATATAGCGGTGCCGCGTCTGGCACTTGGCGCGTTTTTTATCTTCAGATTCAGGAATACTCCGATCCATgg                                                 <  5:162179/134‑1 (MQ=255)                                                                      gCTGGGCAAGTCGCACGCGTGATCGTCCGAGCTTGACCTCACACTTTTGCCGCTATATATAGCGGTGCCGCGTCTGGCACTTGGCGCGTTTTTTATATTCAGATTCAGGAATACTACGATCCATGGGCAAATCGCt                                       >  5:70756/1‑136 (MQ=255)                                                                                  gCACGCGTGATCGTCCGAGCTTGACCTCACACTTTTGCCGCTATATATAGCGGTGCCGCGTCTGGCACTTGGCGCGTTTTTTATCTTCAGATTCAGGAATACTCCGATCCATGGGCAAATCGCTGGTCATTGTgg                            >  2:271629/1‑135 (MQ=255)                                                                                    aCGCGTGATCGTCCGAGCTTGACCTCACACTTTTGCCGCTATATATAGCGGTGCCGCGTCTGGCACTTGGCGCGTTTTTTATCTTCAGATTCAGGAATACTCCGATCCATGGGCAAATCGCTGGTCATTGTGGAAt                         <  1:41458/136‑1 (MQ=255)                                                                                         tGATCGTCCGAGCTTGACCTCACACTTTTGCCGCTATATATAGCGGTGCCGCGTCTGGCACTTGGCGCGTTTTTTATCTTCAGATTCAGGAATACTCCGATCCATGGGCAAATCGCTGGTCATTGTGGAATCCCCg                    <  1:271629/136‑1 (MQ=255)                                                                                         tGATCGTCCGAGCTTGACCTCACACTTTTGCCGCTATATATAGCGGTGCCGCGTCTGGCACTTGGCGCGTTTTTTATCTTCAGATTCAGGAATACTCCGATCCATGGGCAAATCGCTGGTCATTGTGGAATCCCCg                    <  6:333254/136‑1 (MQ=255)                                                                                         tGATCGTCCGAGCTTGACCTCACACTTTTGCCGCTATATATAGCGGTGCCGCGTCTGGCACTTGGCGCGTTTTTTATCTTCAGATTCAGGAATACTCCGATCCATGGGCAAATCGCTGGTCATTGTGGAATCCCCg                    <  4:209878/136‑1 (MQ=255)                                                                                           aTCGTCCGAGCTTGACCTCACACTTTTGCCGCTATATATAGCGGTGCCGCGTCTGGCACTTGGCGCGTTTTTTATCTTCAGATTCAGGAATACTCCGATCCATGGGCAAATCGCTGGTCATTGTGGAATCCCCgg                   <  7:56034/135‑1 (MQ=255)                                                                                                           cTCACACTTTTGCCGCTATATATAGCGGTGCCGCGTCTGGCACTTGGCGCGTTTTTTATCTTCAGATTCAGGAATACTCCGATCCATGGGCAAATCGCTGGTCATTGTGGAATCCCCGGCCAAGGCCAAGACCATc  <  1:197580/136‑1 (MQ=255)                                                                                                           cTCACACTTTTGCCGCTATATATAGCGGTGCCGCGTCTGGCACTTGGCGCGTTTTTTATCTTCAGATTAAGGAATACTCCGATCCATTGGCAAATCGCTGGTCATTGTGGAATCCCCGGCCAAGGCCAAGACCAt   >  8:43901/1‑135 (MQ=255)                                                                                                                         |                                                                                                                          CGTCGAATTTGACGGCGAGCAGGTGCCACGCTTTGCTTTGGTCAAGGCCGAAGAGGGGCTGCCCGGGTTGCTGGGCAAGTCGCACGCGTGATCGTCCGAGCTTGACCTCACACTTTTGCCACTATATATAGCGGTGCCGCGTCTGGCACTTGGCGCGTTTTTTATCTTCAGATTCAGGAATACTCCGATCCATGGGCAAATCGCTGGTCATTGTGGAATCCCCGGCCAAGGCCAAGACCATC  >  ACB143/2433712‑2433953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑