Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB143 3,181,018 G→A A532T (GCC→ACC)  actP_2 → Cation/acetate symporter ActP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1433,181,0180GA96.2% 152.1 / NA 53A532T (GCC→ACC) actP_2Cation/acetate symporter ActP
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (30/21);  minor base C (2/0);  total (32/21)
Fisher's exact test for biased strand distribution p-value = 5.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGATGTGTTGCACTTTGCCGAACCGCTGTTCCCCTATACCCAGCCTGCGCTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGGCCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGATAGCTCGCTAGTAACCTCTGAA  >  ACB143/3180885‑3181148
                                                                                                                                     |                                                                                                                                  
ggATGTGTTGCACTTTGCCGAACCGCTGTTCCCCTATACCCAGCCTGCGCTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGccc                                                                                                                                  >  7:345621/1‑136 (MQ=255)
     tgtTGCACTTTGCCGAACCGCTGTTCCCCTATACCCAGCCTGCGCTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGc                                                                                                                             >  5:305368/1‑136 (MQ=255)
       ttGCACTTTGCCGAACCGCTGTTCCCCTATACCCAGCCTGCGCTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGcc                                                                                                                            <  3:61326/135‑1 (MQ=255)
       ttGCACTTTGCCGAACCGCTGTTCCCCTATACCCAGCCTGCGCTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGcc                                                                                                                            <  6:14128/135‑1 (MQ=255)
                  cGAACCGCTGTTCCCCTATACCCAGCCTGCGCTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTc                                                                                                                >  6:298139/1‑136 (MQ=255)
                  cGAACCGCTGTTCCCCTATACCCAGCCTGCGCTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTc                                                                                                                >  3:350821/1‑136 (MQ=255)
                  cGAACCGCTGTTCCCCTATACCCAGCCTGCGCTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTc                                                                                                                >  7:33963/1‑136 (MQ=255)
                     tccGCTGGTCCCCTATACCCAGACGGCGCTGTTCTCCATGCCGCTGTCGTTTTTGGTGGTGTATGCGGTTTCCCGTAATGACCGCACTGCGCGGGCCTAGGCCGATATTGCGACCTTCGCCGACCAGTGCGTCCGt                                                                                                             <  8:82086/135‑1 (MQ=255)
                            ttCCCCTATACCCAGCCTGCGCTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCa                                                                                                        <  1:331716/134‑1 (MQ=255)
                            ttCCCCTATACCCAGCCTGCGCTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCa                                                                                                        <  5:344221/134‑1 (MQ=255)
                               cccTATACCCAGCCTGCGCTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCg                                                                                                   <  5:298139/136‑1 (MQ=255)
                                    taCCCAGCCTGCGCTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGCCCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCtt                                                                                              >  1:263807/1‑136 (MQ=255)
                                    taCCCAGCCTGCGCTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCtt                                                                                              >  6:298989/1‑136 (MQ=255)
                                         aGCCTGCGCTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGc                                                                                         >  5:256093/1‑136 (MQ=255)
                                            cTGCGCTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGcc                                                                                                                            >  7:304819/1‑98 (MQ=255)
                                            cTGCGCTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGcc                                                                                                                            <  8:304819/98‑1 (MQ=255)
                                            cTGCGCTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAg                                                                                      >  5:146703/1‑136 (MQ=255)
                                            cTGCGCTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAg                                                                                      >  5:176395/1‑136 (MQ=255)
                                            cTGCGCTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAg                                                                                      >  3:346790/1‑136 (MQ=255)
                                              gcgcTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATTCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGCCCTTCGCCGACCAGGTCGTCCGTGGCCAGCCCGGCCGTGGTGCCcgcg                                                                                    >  6:24177/1‑136 (MQ=255)
                                              gcgcTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCg                                                                                    >  7:6315/1‑136 (MQ=255)
                                              gcgcTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCg                                                                                    >  4:239614/1‑136 (MQ=255)
                                              gcgcTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCg                                                                                    >  2:224359/1‑136 (MQ=255)
                                              gcgcTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACAGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGACAGCg                                                                                    >  4:68378/1‑136 (MQ=255)
                                                 cTGTTCTCCATGCCGCTGGCGTTTTTGGTTGCGTATGCGGTTTCCCGGATTGACCGCCGTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGAGCAGTTCGTCCGTGGCCAGACCGGCCGTGGTGCCAGCGGTg                                                                                 >  7:132774/1‑136 (MQ=255)
                                                       tcCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTc                                                                            >  4:163572/1‑135 (MQ=255)
                                                       tcCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCg                                                                           >  3:309648/1‑136 (MQ=255)
                                                               gCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGAACTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTTCCAGCGGTGCCGGCGATCAtt                                                                     >  2:333548/1‑134 (MQ=255)
                                                                           tgtggCGTATGCGGTTTACCGGATGGACCGCAATGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGACAGACCGGCCTTGGTGCAAGCGGTTCCGTCGATCATTGATTTACCCGCGGc                                                       >  1:7105/2‑136 (MQ=255)
                                                                           ggtggCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACAGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGc                                                       >  1:13387/1‑136 (MQ=255)
                                                                                              cGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGc                                      >  2:49746/1‑134 (MQ=255)
                                                                                                 aTGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCg                                 <  7:165595/136‑1 (MQ=255)
                                                                                                       cGCACTGCGCGGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCgtg                           <  5:239938/136‑1 (MQ=255)
                                                                                                           cggcgcGGGCCAAGGCCGAAAGGGCAACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGAt                       <  8:351124/134‑1 (MQ=255)
                                                                                                              cgcgGGCCATGGCCGAAAGGGCGACCTTCGCCTACCAGTTCGTCCGTGGCAAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGGTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGATAGc                    <  6:141039/136‑1 (MQ=255)
                                                                                                              cgcgGGCCAAGGCCGACCGGGCTACCTTCGCCGACCAGTTCGTCCTTGGCCAGACCGGAGGTGGTGCCAGCGGTGCCTTCGAGCATTTAGTTACCCGCGGCCGCATGCGCAGTAGTGCCAGCGGCCGTGTGATa                      <  6:253240/134‑1 (MQ=255)
                                                                                                              cgcgGGCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGATAGc                    <  7:320870/136‑1 (MQ=255)
                                                                                                              cgcgGGCAATGGCCGAAAGGGCTACCTTCGCCGACCATTTCGTCCGGGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCTATAATTGATTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGATAGc                    <  2:152312/136‑1 (MQ=255)
                                                                                                                 gggCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGATAGCTCg                 >  2:301536/1‑136 (MQ=255)
                                                                                                                 gggCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGATAGCTCg                 >  1:162106/1‑136 (MQ=255)
                                                                                                                 gggCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGGGATAGCTCg                 >  3:95569/1‑136 (MQ=255)
                                                                                                                   gcaaaGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTAACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGATAGCTCGCt               <  1:114583/133‑1 (MQ=255)
                                                                                                                   gCCAAGGCCGATAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGATAGCTCGCt               <  1:243072/136‑1 (MQ=255)
                                                                                                                   gCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGATAGCTCGCt               <  4:309648/136‑1 (MQ=255)
                                                                                                                   gCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGATAGCTCGCt               <  7:29391/136‑1 (MQ=255)
                                                                                                                   gCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGATAGCTCGCt               <  4:350821/136‑1 (MQ=255)
                                                                                                                   gCCAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGATAGCTCGCt               <  7:47004/136‑1 (MQ=255)
                                                                                                                    ccAAGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCCGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGATAGCTCGc                >  4:233511/1‑134 (MQ=255)
                                                                                                                      aaGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGATAGCTCGCTAg             >  7:276479/1‑135 (MQ=255)
                                                                                                                      aaGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGATAGCTCGCTAg             >  1:375378/1‑135 (MQ=255)
                                                                                                                      aaGGCCGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGATAGCTCGCTAg             >  2:63198/1‑135 (MQ=255)
                                                                                                                           cGAAAGGGCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCCGCGGCCGTGTGATAGCTCGCTAGTAACct       >  1:272396/1‑136 (MQ=255)
                                                                                                                                gggCGACCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGATAGCTCGCTAGTAACCTCTg    >  5:341471/1‑134 (MQ=255)
                                                                                                                                gggCGAACTTCGCCGACCAGTTCTTCCGTGGCCAGACCGTCCTTGGTGCCAGCTTTGACGTCGATAATTGATTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCTGCCGTGTGATAGCTAGCTAGTAACCTCTGaa  <  4:62624/136‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                  
GGATGTGTTGCACTTTGCCGAACCGCTGTTCCCCTATACCCAGCCTGCGCTGTTCTCCATGCCGCTGGCGTTTTTGGTGGCGTATGCGGTTTCCCGGATGGACCGCACTGCGCGGGCCAAGGCCGAAAGGGCGGCCTTCGCCGACCAGTTCGTCCGTGGCCAGACCGGCCTTGGTGCCAGCGGTGCCGTCGATCATTGAGTTACCCGCGGCCGCAAGCGCAGGAGTGCCAGCGGCCGTGTGATAGCTCGCTAGTAACCTCTGAA  >  ACB143/3180885‑3181148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑