Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB143	3,833,928	G→A	R406R (CGC→CGT)	nicP_8 ←	Porin‑like protein NicP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	3,833,928	0	G	A	100.0%	240.3 / NA	71	R406R (CGC→CGT)	nicP_8	Porin‑like protein NicP
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/48);  total (23/48)

CAGATCCAAAGCGCAAGCGAAACCGGCTGCGCCCAACGAAGATTGTGTCTGAATTCAGAATTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAGCGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACTGCACCACATAGGTGATATCCGTAGTCCGCTCCCATTCCCGCCCCTCTCCGTCGCCAGCGGCCAATGCCCTGCCCTTG  >  ACB143/3833811‑3834057                                                                                                                      |                                                                                                                                  cAGCTCCAAAGCGCAAGCGAAACCGGCTGCGCCCAACGAAGATTGTGTCTGAATTCAGAATTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTgcgc                                                                                                                 <  6:262175/136‑1 (MQ=255)      ccAACGCGCAAGCGAAACCGGCTGCGCCCAACTAAGATTGTGTCTGAATTCAGAATTCGAACGTGTAGCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACg                                                                                                            <  2:168545/136‑1 (MQ=255)       cAAAGCGCAAGCGAAACCGGCTGCGCCCAACGAAGATTGTGTCTGAATTCAGAATTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACg                                                                                                            <  1:121966/135‑1 (MQ=255)       cAAAGCGCAAGCGAAACCGGCTGCGCCCAACGAAGATTGTGTCTGAATTCAGAATTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACg                                                                                                            <  7:25626/135‑1 (MQ=255)       cAAAGCGCAAGCGAAACCGGCTGCGCCCAACGAAGATTGTGTCTGAATTCAGAATTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACg                                                                                                            <  5:147538/135‑1 (MQ=255)       cAAAGCGCAAGCGAAACCGGCTGCGCCCAACGAAGATTGTGTCTGAATTCAGAATTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACg                                                                                                            <  3:286474/135‑1 (MQ=255)       cAAAGCGCAAGCGAAACCGGCTGCGCCCAACGAAGATTGTGTCTGAATTCAGAATTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACg                                                                                                            <  2:370647/135‑1 (MQ=255)       cAAAGCGCAAGCGAAACCGGCTGCGCCCAACGAAGATTGTGTCTGAATTCAGAATTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATACGAACGCATGCTCGCATTGCGCCAACg                                                                                                            >  2:325763/1‑135 (MQ=255)           gcgcAAGCGAAACCGGCTGCGCCCAACGAAGATTGTGTCTGAATTCAGAATTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCTTGCTCGCATTGCGCCAACGCAGCg                                                                                                       <  6:171425/136‑1 (MQ=255)           cggcATGCGAAACCGGCTGCGCCCAACGAAGATTGTGTCTGAATTCAGAATTCGAAGTTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCg                                                                                                       <  1:160702/134‑1 (MQ=255)            cgcAAGCGAAACCGGCTGCGCCCAACGAAGATTGTGTCTGAATTCAGAATTCGAACGTGTACCCCACGATCACCAGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGa                                                                                                      >  2:111235/1‑136 (MQ=255)                 gCGAAACCGGCTGCGCCCAACGAAGATTGTGTCTGAATTCAGAATTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGAcacag                                                                                                 >  3:128713/1‑135 (MQ=255)                 gCGAAACCGGCTGCGCCCAACGAAGATTGTGTCTGAATTCAGAATTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACAt                                                                                                 >  3:149158/1‑136 (MQ=255)                 gCGAAACCGGCTGCGCCCAACGAAGATTGTGTCTGAATTCAGAATTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACAt                                                                                                 >  6:83895/1‑136 (MQ=255)                 gCGAAACCGGCTGCGCCCAACGAAGATTGTGTCTGAATTCAGAATTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACAt                                                                                                 >  2:75400/1‑136 (MQ=255)                 gCGAAACCGGCTGCGCCCAACGAAGATTGTGTCTGAATTCAGAATTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACAt                                                                                                 >  6:133907/1‑136 (MQ=255)                     aaCCGGCTGCGCCCAACGAAGATTGTGTCTTAATTCATAATTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCt                                                                                             <  7:25054/136‑1 (MQ=255)                         ggCTGCGCCCAACGAAGATTGTGTCTGAATTCAGAATTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAgc                                                                                         >  8:46628/1‑136 (MQ=255)                         ggCTGCGCCCAACGAAGATTGTGTCTGAATTCAGAATTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAgc                                                                                         <  1:363901/136‑1 (MQ=255)                              cgcCCAACGAAGATTGTGTCTGAATTCAGAATTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGcccc                                                                                    <  1:61768/136‑1 (MQ=255)                              cgcCCAACGAAGATTGTGTCTGAATTCAGAATTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGcccc                                                                                    >  2:86885/1‑136 (MQ=255)                              cgcCCAACGAAGATTGTGTCTGAATTCAGAATTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGcccc                                                                                    <  7:160423/136‑1 (MQ=255)                              cgaccAACGAAGATTTTGTGTGAATTAAGAATTCGAACGTTTACGCCACGATCACCCGGTTCTCATACGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGcccc                                                                                    <  8:283581/133‑1 (MQ=255)                                 ccAACGAAGATTGTGTCTGAATTCAGAATTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGa                                                                                 <  6:347380/136‑1 (MQ=255)                                   aaCGAAGATTGTGTCTGAATTCAGAATTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACt                                                                               <  6:223899/136‑1 (MQ=255)                                   aaCGAAGATTGTGTCTGAATTCAGAATTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACt                                                                               <  3:89491/136‑1 (MQ=255)                                   aaCGAAGATTGTGTCTGAATTCAGAATTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACt                                                                               <  1:117055/136‑1 (MQ=255)                                   aaCGAAGATTGTGTCTGAATTCAGAATTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACt                                                                               <  7:46628/136‑1 (MQ=255)                                   aaCGAAGATTGTGTCTGAATTCAGAATTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACt                                                                               <  8:110052/136‑1 (MQ=255)                                   aaCGAAGATTGTGTCTGAATTCAGAATTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACt                                                                               >  8:285047/1‑136 (MQ=255)                                   aaCGAAGATTGTGTCTGAATTCAGAATTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACt                                                                               <  8:134255/136‑1 (MQ=255)                                    aCGAAGATTGTGTCTGAATTCAGAATTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACTg                                                                              <  5:161421/136‑1 (MQ=255)                                    aCGAAGATTGTGTCTGAATTCAGAATTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACTg                                                                              <  3:137139/136‑1 (MQ=255)                                        cgATTGTGGCTGAATTCAGAATTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACTGcacc                                                                          <  3:326822/135‑1 (MQ=255)                                        aGATTGTGTCTGAATTCAGAATTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACTGcacc                                                                          <  1:174099/136‑1 (MQ=255)                                        aGATTGTGTCTGAATTCAGAATTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACTGcacc                                                                          <  3:80546/136‑1 (MQ=255)                                        aGATTGTGTCTGAATTCAGAATTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACTGcacc                                                                          <  2:290488/136‑1 (MQ=255)                                        aGATTGTGTCTGAATTCAGAATTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACTGcacc                                                                          <  6:28552/136‑1 (MQ=255)                                                tCTGAATTCAGAATTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACTGCACCACATAgg                                                                   <  6:103797/135‑1 (MQ=255)                                                tCTGAATTCAGAATTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACTGCACCACATAGGt                                                                  >  3:335402/1‑136 (MQ=255)                                                       tCAGAATTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACTGCACCACATAGGTGATATcc                                                           >  6:309617/1‑136 (MQ=255)                                                          gAATTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACTGCACCACATAGGTGATATCCGTa                                                        <  3:221291/136‑1 (MQ=255)                                                           aaTTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACTGCACCACATAGGTGATATCCGTAg                                                       >  8:37204/1‑136 (MQ=255)                                                              tCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACTGCACCACATAGGTGATATCCGTAGt                                                      >  5:4169/1‑134 (MQ=255)                                                                   cGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACTGCACCACATAGGTGATATCCGTAGTCCGCTcc                                               <  6:285273/136‑1 (MQ=255)                                                                       tACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCACCCGACTGCACCACATAGGTGATATCCGTAGTCCGCTCCCAtt                                           >  3:89421/1‑136 (MQ=255)                                                                               gATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACTGcacc                                                                          >  8:9750/1‑97 (MQ=255)                                                                               gATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACTGcacc                                                                          <  7:9750/97‑1 (MQ=255)                                                                               gATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACTGCACCACATAGGTGATATCCGTAGTCCGCTCCCATTCCCGccc                                    >  5:76336/1‑135 (MQ=255)                                                                                   aCCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACTGCACCACATAGGTGATATCCGTAGTCCGCTCCCATTCCCGCCCCtctc                               <  3:269136/136‑1 (MQ=255)                                                                                       ggTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACTGCACCACATAGGTGATATCCGTAGTCCGCTCCCATTCCCGCCCCTCTCCGTc                           >  2:185382/1‑136 (MQ=255)                                                                                       ggTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACTGCACCACATAGGTGATATCCGTAGTCCGCTCCCATTCCCGCCCCTCTCCGTc                           <  5:309617/136‑1 (MQ=255)                                                                                       ggTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACTGCACCACATAGGTGATATCCGTAGTCCGCTCCCATTCCCGCCCCTCTCCGTc                           >  3:28458/1‑136 (MQ=255)                                                                                        gTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACTGCACCACATAGGTGATATCCGTAGTCCGCTCCCATTCCCGCCCCTCTCCGTCg                          <  8:82130/136‑1 (MQ=255)                                                                                        gTTCTCATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACTGCACCACATAGGTGATATCCGTAGTCCGCTCCCATTCCCGCCCCTCTCCGTCg                          <  4:119403/136‑1 (MQ=255)                                                                                           ctcATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACTGCACCACATAGGTGATATCCGTAGTCCGCTCCCATTCGCGCCCCTCTCCGTCGcc                        <  5:15097/135‑1 (MQ=255)                                                                                           ctcATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACTGCACCACATAGGTGATATCCGTAGTCCGCTCCCATTCCCGCCCCTCTCCGTCGcc                        <  1:166305/135‑1 (MQ=255)                                                                                           ctcATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACTGCACCACATAGGTGATATCCGTAGTCCGCTCCCATTCCCGCCCCTCTCCGTCGcc                        <  4:177497/135‑1 (MQ=255)                                                                                           ctcATCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACTGCACCACATAGGTGATATCCGTAGTCCGCTCCCATTCCCGCCCCTCTCCGTCGcc                        <  7:294213/135‑1 (MQ=255)                                                                                               tCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACTGCACCACATAGGTGATATCCGTAGTCCGCTCCCATTCCCGCCCCTCTCCGTCGCCAGCgg                   <  5:99690/136‑1 (MQ=255)                                                                                               tCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACTGCACCACATAGGTGATATCCGTAGTCCGCTCCCATTCCCGCCCCTCTCCGTCGCCAGCgg                   <  1:68092/136‑1 (MQ=255)                                                                                               tCCGCTGCATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACTGCACCACATAGGTGATATCCGTAGTCCGCTCCCATTCCCGCCCCTCTCCGTCGCCAGCgg                   <  5:37702/136‑1 (MQ=255)                                                                                                      cATCGGCAAAGTTCGAAGGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACTGCACGACATAGGTGATATCCGTAGTCCGCTCCCATTCCCGCCCCTCTCCGTCGCCAGCGGCCAAt              <  5:19630/134‑1 (MQ=255)                                                                                                      cATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACTGCACCACATAGGTGATATCCGTAGTCCGCTCCCATTCCCGCCCCTCTCCGTCGCCAGCGGCCAAtgc            >  5:160650/1‑136 (MQ=255)                                                                                                      cATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACTGCACCACATAGGTGATATCCGTAGTCCGCTCCCATTCCCGCCCCTCTCCGTCGCCAGCGGCCAAtg             <  5:261987/135‑1 (MQ=255)                                                                                                      cATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACTGCACCACATAGGTGATATCCGTAGTCCGCTCCCATTCCCGCCCCTCTCCGTCGCCAGCGGCCAAt              <  7:329428/134‑1 (MQ=255)                                                                                                      cATCGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACTGCACCACAAAGGTGATATCCGTAGTCCGCTCCCATTCCCGCCCCTCTCCGTCGCCAGCGGCCAAtgc            >  5:237452/1‑136 (MQ=255)                                                                                                      cATCGGCAAAATTCGAACGCATGCTCGAATTGCGCCACCGCAGCGACACATTCCTGAGCGCCCCCGACTGCACCACATAGGTGATATCCGTAGTCCGCTCCCATTCCCGCCCCTCTCCGTCGCCCGCGGCCAAtgc            >  8:209073/1‑136 (MQ=255)                                                                                                         cGGCAAAATTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACTGCACCACATAGGTGATATCCGTAGTCCGCTCCCATTCCCGCCCCTCTCCGTCGCCAGCGGCCAAtgccct         >  5:358531/1‑136 (MQ=255)                                                                                                                aatCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACTGCACCACATAGGTGATATCCGTAGTCCGCTCCCATTCCCGCCCCTCTCCGTCGCCAGCGGCCAATGCCCTGCCCTTg  <  2:139465/134‑1 (MQ=255)                                                                                                                aTTCGAACGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACTGCACCACATAGGTGATATCCGTAGTCCGCTCCCATTCCCGCCCCTCTCCGTCGCCAGCGGCCAATGCCCTGCCCTTg  <  5:84179/136‑1 (MQ=255)                                                                                                                      |                                                                                                                                  CAGATCCAAAGCGCAAGCGAAACCGGCTGCGCCCAACGAAGATTGTGTCTGAATTCAGAATTCGAACGTGTACCCCACGATCACCCGGTTCTCATCCGCTGCATCGGCAAAATTCGAGCGCATGCTCGCATTGCGCCAACGCAGCGACACATTCCTGAGCGCCCCCGACTGCACCACATAGGTGATATCCGTAGTCCGCTCCCATTCCCGCCCCTCTCCGTCGCCAGCGGCCAATGCCCTGCCCTTG  >  ACB143/3833811‑3834057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑