Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB143 3,994,687 C→T S247S (AGC→AGT dmoA_3 → Dimethyl‑sulfide monooxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1433,994,6870CT97.4% 233.7 / NA 79S247S (AGC→AGTdmoA_3Dimethyl‑sulfide monooxygenase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (42/34);  minor base G (0/2);  total (43/36)
Fisher's exact test for biased strand distribution p-value = 2.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGAGCACTTCCAGGTGCCCGGCATGCACCTGTGCCAGCCATCGCCGCAACGCACCCCGGTGTTGTTCCAGGCCGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGCGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGCGGCCGGGCGTGGCCGTGACGAAGTGCTGATCTACGCCCAGGCGCTGTTGATCGTCGCCCCCACGCGGGAAGAG  >  ACB143/3994554‑3994819
                                                                                                                                     |                                                                                                                                    
gCGAGCACTTCCAGGTGCCCGGCATGCACCTGTGCCAGCCATCGCCGCAACTCACCCCGGTGTTGTTCCTGGCCGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTgg                                                                                                                                    <  4:290254/136‑1 (MQ=255)
gCGAGCACTTCCAGGTGCCCGGCATGCACCTGTGCCAGCCATCGCCGCAACGCACCCCGGTGTTGTTCCAGGCCGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTgg                                                                                                                                    >  3:350863/1‑136 (MQ=255)
gCGAGCACTTCCAGGTGCCCGGCATGCACCTGTGCCAGCCATCGCCGCAACGCACCCCGGTGTTGTTCCAGGCCGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTgg                                                                                                                                    >  3:199715/1‑136 (MQ=255)
gCGAGCACTTCCAGGTGCCCGGCATGCACCTGTGCCAGCCATCGCCGCAACGCACCCCGGTGTTGTTCCAGGCCGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTgg                                                                                                                                    <  4:211446/136‑1 (MQ=255)
   aGCACTTCCAGGTGCCCGGCATGCACCTGTGCCAGCCATCGCCGCAACGCACCCCGGTGTTGTTCCAGGCCGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGc                                                                                                                                  >  8:43840/1‑135 (MQ=255)
      aCTTCCAGGTGCCCGGCATGCACCTGTGCCAGCCATCGCCGCAACGCACCCCGGTGTTGTTCCAGGCCGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAAc                                                                                                                              >  1:315001/1‑136 (MQ=255)
      aCTTCCAGGTGCCCGGCATGCACCTGTGCCAGCCATCGCCGCAACGCACCCCGGTGTTGTTCCAGGCCGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAAc                                                                                                                              >  4:304749/1‑136 (MQ=255)
          ccAGGTGCCCGGCATGCCCCGGGGCCTGCCATCCCCGCAACGCACCCCGGTGTTGTTCCCGGCCGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGc                                                                                                                            <  8:312790/134‑1 (MQ=255)
          ccAGGTGCCCGGCATGCACCTGTGCCAGCCATCGCCGCAACGCACCCCGGTGTTGTTCCAGGCCGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGc                                                                                                                            <  5:152396/134‑1 (MQ=255)
          ccAGGTGCCCGGCATGCACCTGTGCCAGCCATCGCCGCAACGCACCCCGGTGTTGTTCCAGGCCGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGc                                                                                                                            <  5:128401/134‑1 (MQ=255)
          ccAGGTGCCCGGCATGCACCTGTGCCAGCCATCGCCGCAACGCACCCCGGTGTTGTTCCAGGCCGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGc                                                                                                                            <  2:64085/134‑1 (MQ=255)
                  ccGGCATGCACCTGTGCCAGCCATCGCCGCAACGCCCCCCGGTGTTGTTCCAGGCCGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCACCGCCGACGGtgct                                                                                                                  >  1:21721/1‑136 (MQ=255)
                     gCATGCACCTGTGCCAGCCATCGCCGCAACGCACCCCGGTGTTGTTCCAGGCCGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTgcg                                                                                                               >  2:185016/1‑136 (MQ=255)
                     gCATGCACCTGTGCCAGCCATCGCCGCAACGCACCCCGGTGTTGTTCCAGGCCGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTgcg                                                                                                               >  7:89688/1‑136 (MQ=255)
                     gCATGCACCTGTGCCAGCCATCGCCGCAACGCACCCCGGTGTTGTTCCAGGCCGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTgcg                                                                                                               >  7:84456/1‑136 (MQ=255)
                     gCATGCACCTGTGCCAGCCATCGCCGCAACGCACCCCGGTGTTGTTCCAGGCCGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTgcg                                                                                                               >  8:353714/1‑136 (MQ=255)
                     gCATGCACCTGTGCCAGCCATCGCCGCAACGCACCCCGGTGTTGTTCCAGGCCGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTgcg                                                                                                               >  5:19412/1‑136 (MQ=255)
                     gCATGCACCTGTGCCAGCCATCGCCGCAACGCACCCCGGTGTTGTTCCAGGCCGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTACATCAGTGGGCCAACGCCGCCGGTGCTgcg                                                                                                               >  2:261884/1‑136 (MQ=255)
                     gCATGCACCTGTGCCAGCCATCGCCGCAACGCACCCCGGTGTTGTTCCAGGCCGGTGCGTCGGCACGCGGACAGCAGTTTGCCGCCCGGCATGCCGACTGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTgcg                                                                                                               >  3:278426/1‑136 (MQ=255)
                       aTGCACCTGTGCCAGCCATCGCCGCAACGCACCCCGGTGTTGTTCCAGGCCGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTGcgcc                                                                                                             >  7:334530/1‑136 (MQ=255)
                                   ccgccaGCGCCGCAACCCACCCCGGGGTGGTTCCAGGCCGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGGGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACg                                                                                                 <  6:204636/134‑1 (MQ=255)
                                   cagccagcGCCGCAACGCACCCCGGTGTTGTGCCAGGCCGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACg                                                                                                 <  2:309877/136‑1 (MQ=255)
                                   cagccaTCGCCGCAACGCACCCTGGTGTTGTTCCAGGCCGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACg                                                                                                 >  5:225938/1‑136 (MQ=255)
                                   cagccaTCGCCGCAACGCACCCCGGTGTTGTTCCAGGCCGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCACCGCCGACGGTGCTGCGCCGCTACGCCGACg                                                                                                 >  4:23749/1‑136 (MQ=255)
                                   cagccaTCGCCGCAACGCACCCCGGTGTTGTTCCAGGCCGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACg                                                                                                 >  7:10851/1‑136 (MQ=255)
                                   cagccaTCGCCGCAACGCACCCCGGTGTTGTTCCAGGCCGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACg                                                                                                 <  8:196517/136‑1 (MQ=255)
                                   cagccaTCGCCGCAACGCACCCCGGTGTTGTTCCAGGCCGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACg                                                                                                 >  1:23526/1‑136 (MQ=255)
                                   cagccaTCGCCGCAACGCACCCCGGTGTTGTTCCAGGCCGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACg                                                                                                 >  5:263978/1‑136 (MQ=255)
                                     gccaTCGCCGCAACGCACCCCGGTGTTGTTCCAGGCCGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACgg                                                                                                >  4:205468/1‑135 (MQ=255)
                                        aTCGCCGCAACGCCCCCGGGTGTTGATCCAGCCGGGTGCGTCGGCACGCGGCCAGCATTTTGCCGCACGGCTTGCCGAATGGGTGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATc                                                                                            <  6:39472/136‑1 (MQ=255)
                                        aTCGCCGCAACGCACCCCGGTGTTGTTCCAGGCCGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATc                                                                                            <  8:208279/136‑1 (MQ=255)
                                        aTCGCCGCAACGCACCCCGGTGTTGTTCCAGGCCGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATc                                                                                            >  8:210559/1‑136 (MQ=255)
                                             cgcAACGCACCCCGGTGTTGTTCCAGGCCGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGAATCCGccc                                                                                       >  3:26179/1‑135 (MQ=255)
                                                    cACCCCGGGGTTGTGCCCGGCCGGGGCGGCGGCACGCGGCCAGCGGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGc                                                                                <  3:265814/136‑1 (MQ=255)
                                                     aCCCCGGTGTGGTTCCAGGCCGGTGCGGCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGGGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCg                                                                               <  5:85208/136‑1 (MQ=255)
                                                         cGGTGTTGTGCCAGGCCGGTGCGGCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGGTCATCAGTGGGCCACCGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGc                                                                           <  6:88483/136‑1 (MQ=255)
                                                          ggTGTTGTTCCAGGCCGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGCg                                                                          >  5:294318/1‑136 (MQ=255)
                                                          ggTGTTGTTCCAGGCCGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGCGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGCg                                                                          >  7:256890/1‑136 (MQ=255)
                                                            tgttgtTCCAGGCCGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGCGGc                                                                        >  5:921/1‑136 (MQ=255)
                                                              tggttCCAGGCCGGGGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGCGGCCg                                                                      <  3:88000/134‑1 (MQ=255)
                                                               tgttCCAGGCCGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGCGGCCgg                                                                     >  8:175569/1‑136 (MQ=255)
                                                               tgttCCAGGCCGGTGCGTCGGCACGCGGCCAGAAGTTTGCCGCCCGGCATGCCGAATGCGTTTTCATAAGTGGGCCAACGCCGACGTTGCTGCGCCGATACGCCGACGGAATCCGCCAGGCATCCGAAGCGCCCgg                                                                     >  8:187699/1‑136 (MQ=255)
                                                               tgttACACGCAGGTGCGTCGGCACGAGGCCAGCAGTTTGCCGCACGGCATGCAGATAGCGAGTTCATCAGTGTGCAAACGCCGACGGTAATGCGCCGCTACTCCGACCGCATCCGCCAGACCTGCGAAGAGGCCgg                                                                     >  8:300208/1‑136 (MQ=255)
                                                                 ttCCAGGCCGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGCGGCCggg                                                                    <  1:587/135‑1 (MQ=255)
                                                                  tCCAGGCCGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGCGGCCGGGCg                                                                  >  4:87380/1‑136 (MQ=255)
                                                                    ccgcccGGGGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAAGGCGTGGTCATCCGGGGGCCACCGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGCGGCCGGGCGTg                                                                <  3:211466/132‑1 (MQ=255)
                                                                    cAGGCCGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGCGGCCGGGCGTg                                                                >  5:96718/1‑136 (MQ=255)
                                                                         cGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGCGGCCGGGCGTGGCCGt                                                           >  2:15488/1‑136 (MQ=255)
                                                                          ggTGGGTCGCCACGCGGCCAGCAGTTTTCCGCACGGCTTGCCGAATGCGTGTTCTGCTGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCGGCCAGGCCTGCGAAGCGGCCGGGCGTGGCCGTg                                                          <  1:380005/136‑1 (MQ=255)
                                                                          ggTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGCGGCCGGTCGTGGCCGTg                                                          <  5:181037/136‑1 (MQ=255)
                                                                          ggTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGCGGCCGGGCGTGGCCGTg                                                          <  3:368209/136‑1 (MQ=255)
                                                                          ggTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGCGGCCGGGCGTGGCCGTg                                                          <  4:218450/136‑1 (MQ=255)
                                                                              cGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGATGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGCGGCCGGGCGTGGCCGTGACg                                                       >  1:40574/1‑135 (MQ=255)
                                                                              cGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGCGGCCGGGCGTGGCCGTGACg                                                       >  7:210439/1‑135 (MQ=255)
                                                                               gTCGGCACGCGGCAAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGCGGCCGGGCGTGGCCGTGACGaa                                                     <  3:86203/136‑1 (MQ=255)
                                                                                 gggCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGTATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGCGGCCGGGCGTGGCCGTGACGAAGt                                                   <  3:9992/135‑1 (MQ=255)
                                                                                    cACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAAGGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGCGGCCGGGCGTGGCCGTGACGAAGTGCt                                                <  6:26518/136‑1 (MQ=255)
                                                                                         ggCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTCCGAAGCCGCCGCGCGTGGCCGTGCCGAAGTGCTGATc                                            >  2:340594/1‑135 (MQ=255)
                                                                                               cagTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGCGGCCGGGCGTGGCCGTGACGAAGTGCTGATCTACGccc                                     <  6:294187/136‑1 (MQ=255)
                                                                                               cagTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGCGGCCGGGCGTGGCCGTGACGAAGTGCTGATCTACGccc                                     <  5:363063/136‑1 (MQ=255)
                                                                                               cagTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGCGGCCGGGCGTGGCCGTGACGAAGTGCTGATCTACGccc                                     <  3:374104/136‑1 (MQ=255)
                                                                                               cagTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGCGGCCGGGCGTGGCCGTGACGAAGTGCTGATCTACGccc                                     <  7:175569/136‑1 (MQ=255)
                                                                                               cagTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGCGGCCGGGCGTGGCCGTGACGAAGTGCTGATCTACGccc                                     <  8:265041/136‑1 (MQ=255)
                                                                                               cagTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGCGGCCGGGCGTGGCCGTGACGAAGTGCTGATCTACGcc                                      <  8:264264/135‑1 (MQ=255)
                                                                                               cagTTTGCCGCACGGCATGCCGAAGGCGTGTTCATCAGGGGGCCACCGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGCGGCCGGGCGTGGCCGTGACGAAGTGCTGATCTACGccc                                     <  6:209621/136‑1 (MQ=255)
                                                                                                 gTTTGCCGCACGGCAGGCCGAATGCGGGTGCAGCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGCGGCCGGGCGTGGCCGTGACGAAGTGCTGATCTACGCCCAg                                   <  7:281480/136‑1 (MQ=255)
                                                                                                    tGCCGCACGGCATGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGCGGCCGGGCGTGGCCGTGACGAAGTGCTGATCTACGCCCAGgcg                                >  1:357026/1‑136 (MQ=255)
                                                                                                          aCGGCGTGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGCGGCCGGGCGTGGCCGTGACGAAGTGCTGATCTACGCCCAGGCGCTGtt                           >  8:180556/1‑135 (MQ=255)
                                                                                                            ggCATGCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGCGGCCGGGCGTGGCCGTGACGAAGTGCTGATCTACGCCCAGGCGCTGTTGAt                        <  6:225938/136‑1 (MQ=255)
                                                                                                                 gCCGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGCGGCCGGGCGTGGCCGTGACGAAGTGCTGATCTACGCCCAGGCGCTGTTGAtcgtcg                   >  1:21191/1‑136 (MQ=255)
                                                                                                                   cGAATGCGTGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGCGGCCGGGCGTGGCCGTGACGAAGTGCTGATCTACGCCCAGGCGCTGTTGATCGTCGcc                 >  6:362129/1‑136 (MQ=255)
                                                                                                                         cGTGTTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGCGGCCGGGCGTGGCCGTGACGAAGTGCTGATCTACGCCCAGGCGCTGTTGATCGTCGCCCCCAc            <  4:208258/135‑1 (MQ=255)
                                                                                                                          gtgtTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGCGGCCGGGCGTGGCCGTGACGAAGTGCTGATCTACGCCCAGGCGCTGTTGATCGTCGCCCCCAcgc          >  7:287918/1‑136 (MQ=255)
                                                                                                                          gtgtTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGCGGCCGGGCGTGGCCGTGACGAAGTGCTGATCTACGCCCAGGCGCTGTTGATCGTCGCCCCCAcgc          >  1:263616/1‑136 (MQ=255)
                                                                                                                            gtTCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGCGGCCGGGCGTGGCCGTGACGAAGTGCTGATCTACGCCCAGGCGCTGTTGATCGTCGCCCCCACGCgg        <  2:320421/136‑1 (MQ=255)
                                                                                                                             ttCATCAGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGCGGCCGGGCGTGGCCGTGACGAAGTGCTGATCTACGCCCAGGCGCTGTTGATCGTCGCCCCCACGCggg       <  6:103643/136‑1 (MQ=255)
                                                                                                                              tcatcaGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGCCATCCGCCAGGCCAGCGAAGCCGCCGGGCGTGGCCGTGACGAAGTGCTGATCTACGCCCAGGCCCTGTTGATCGTAGCCCCCACGCggg       >  8:61043/1‑135 (MQ=255)
                                                                                                                                atcaGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGCGGCCGGGCGTGGCCGTGACGAAGTGCTGATCTACGCCCAGGCGCTGTTGATCGTCGCCCCCACGCggg       >  4:306087/1‑133 (MQ=255)
                                                                                                                                  caGTGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGCGGCCGGGCGTGGCCGTGACGAAGTGCTGATCTACGCCCAGGCGCTGTTGATCGTCGCCCCCACGCGGGAagag  >  3:110970/1‑136 (MQ=255)
                                                                                                                                     |                                                                                                                                    
GCGAGCACTTCCAGGTGCCCGGCATGCACCTGTGCCAGCCATCGCCGCAACGCACCCCGGTGTTGTTCCAGGCCGGTGCGTCGGCACGCGGCCAGCAGTTTGCCGCACGGCATGCCGAATGCGTGTTCATCAGCGGGCCAACGCCGACGGTGCTGCGCCGCTACGCCGACGGCATCCGCCAGGCCTGCGAAGCGGCCGGGCGTGGCCGTGACGAAGTGCTGATCTACGCCCAGGCGCTGTTGATCGTCGCCCCCACGCGGGAAGAG  >  ACB143/3994554‑3994819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: