Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB143	4,053,106	A→G	L474P (CTG→CCG)	pgm ←	Phosphoglucomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	4,053,106	0	A	G	96.0%	261.3 / 0.8	75	L474P (CTG→CCG)	pgm	Phosphoglucomutase
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base G (37/35);  total (38/37)
Fisher's exact test for biased strand distribution p-value = 6.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.13e-01

GTAGATGTCTTCAGTACCCGACGGGCGCGCGGCGAACCAGCCATTGGCCGTCATCACCTTAAGCCCGCCAATCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCAGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGACACGGGTGGCGAACGGCTTGCCCAGCGCCTCGGTCAGGTCGGCATAAGCCTGGCTCG  >  ACB143/4052979‑4053238                                                                                                                                |                                                                                                                                     gTAGATGTCTTCAGTACCCGACGGGCGCGCGGCGAACCAGCCATTGGCCGTCATCACCTTAAGCCCGCCAATCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGct                                                                                                                                   <  8:207384/131‑1 (MQ=255)    gATGTCTTCAGTACCCGACGGGCGCGCGGCGAACCAGCCATTGGCCGTCATCACCTTAAGCCCGCCAATCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGAc                                                                                                                           >  5:127930/1‑136 (MQ=255)    gATGTCTTCAGTACCCGACGGGCGCGCGGCGAACCAGCCATTGGCCGTCATCACCTTAAGCCCGCCAATCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGAc                                                                                                                           >  3:266852/1‑136 (MQ=255)          ttCAGTACCCGACGGGCGCGCGGCGAACCAGCCATTGGCCGTCATCACCTTAAGCCCGCCAATCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCAcc                                                                                                                     >  1:241305/1‑136 (MQ=255)           tCAGTACCCGACGGGCGCGCGGCGAACCCGCCATTGGCCGTCATCACCTTAAGCCCGCCAATCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCt                                                                                                                    >  7:79015/1‑136 (MQ=255)           tCAGTACCCGACGGGCGCGCGGCGAACCAGCCATTGGCCGTCATCACCTTAAGCCCGCCAATCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCt                                                                                                                    >  2:116374/1‑136 (MQ=255)             aGTACCCGACGGGCGCGCGGCGAACCAGCCATTGGCCGTCATCACCTTAAGCCCGCCAATCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGc                                                                                                                  >  6:339496/1‑136 (MQ=255)             aGTACCCGACGGGCGCGCGGCGAACCAGCCATTGGCCGTCATCACCTTAAGCCCGCCAATCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCAGCTCTGTCGACTTCACCTGc                                                                                                                  >  3:263340/1‑136 (MQ=255)               tACCCGACGGGCGCGCGGCGAACCAGCCATTGGCCGTCATCACCTTAAGCCCGCCAATCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTc                                                                                                                >  6:87392/1‑136 (MQ=255)                 cccGACGGGCGCGCGGCGAACCAGCCATTGGCCGTCATCACCTTAAGCCCGCCAATCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCg                                                                                                              <  4:198136/136‑1 (MQ=255)                 cccGACGGGCGCGCGGCGAACCAGCCATTGGCCGTCATCACCTTAAGCCCGCCAATCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCg                                                                                                              <  5:232569/136‑1 (MQ=255)                  ccGACGGGCGCGCGGCGAACCAGCCATTGGCCGTCATCACCTTAAGCCCGCCAATCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCgg                                                                                                             >  2:28907/1‑136 (MQ=255)                       gggCGCGCGGCGAACCAGCCATTGGCCGTCATCACCTTAAGCCCGCCAATCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCa                                                                                                        >  4:107188/1‑136 (MQ=255)                       gggCGCGCGGCGAACCAGCCATTGGCCGTCATCACCTTAAGCCCGCCAATCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTCCTCCGGCGCCa                                                                                                        >  5:240167/1‑136 (MQ=255)                           gcgcgGCGAACCAGCCATTGGCCGTCATCACCTTAAGCCCGCCAATCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCtt                                                                                                    <  2:215349/136‑1 (MQ=255)                            cgcgGCGAACCAGCCATTGGCCGTCATCACCTTAAGCCCGCCAATCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCCGCTTg                                                                                                   >  3:213881/1‑136 (MQ=255)                            cgcgGCGAACCAGCCATTGGCCGTCATCACCTTAAGCCCGCCAATCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTg                                                                                                   >  2:96296/1‑136 (MQ=255)                               ggcgAACCAGCCATTGGCCGTCATCACCTTAAGCCCGCCAATCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCt                                                                                                 >  8:39156/1‑135 (MQ=255)                                gcgAACCAGCCATTGGCCGTCATCACCTTAAGCCCGCCAATCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCa                                                                                               <  8:107659/136‑1 (MQ=255)                                gcgAACCAGCCATTGGCCGTCATCACCTTAAGCCCGCCAATCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCa                                                                                               <  7:140520/136‑1 (MQ=255)                                gcgAACCAGCCATTGGCCGTCATCACCTTAAGCCCGCCAATCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCa                                                                                               <  1:371080/136‑1 (MQ=255)                                gcgAACCAGCCATTGGCCGTCATCACCTTAAGCCCGCCAATCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCa                                                                                               <  1:238528/136‑1 (MQ=255)                                gcgAACCAGCCATTGGCCGTCATCACCTTAAGCCCGCCAATCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCa                                                                                               <  3:128976/136‑1 (MQ=255)                                gcgAACCAGCCATTGGCCGTCATCACCTTAAGCCCGCCAATCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCa                                                                                               <  3:16068/136‑1 (MQ=255)                                           aTTGGCCGTCATCACCTTAAGCCCGCCAATCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTc                                                                                    <  4:195113/136‑1 (MQ=255)                                                  gTCATCACCTTAAGCCCGCCAATCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCcgcg                                                                             <  5:12207/136‑1 (MQ=255)                                                  gTCATCACCTTAAGCCCGCCAATCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCcgcg                                                                             <  4:144515/136‑1 (MQ=255)                                                  gTCATCACCTTAAGCCCGCCAATCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCcgcg                                                                             <  1:168514/136‑1 (MQ=255)                                                  gTCATCACCTTAAGCCCGCCAATCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCcgcg                                                                             <  5:199494/136‑1 (MQ=255)                                                      tcaCCTTAAGCCCGCCAATCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATc                                                                         >  1:331231/1‑136 (MQ=255)                                                        aCCTTAAGCCCGCCAATCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCgg                                                                       <  7:200538/136‑1 (MQ=255)                                                         ccTTAAGCCCGCCAATCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCAGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGc                                                                      <  4:263340/136‑1 (MQ=255)                                                                cccGCCAATCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGcc                                                               >  5:241102/1‑136 (MQ=255)                                                                cccGCCAATCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGcc                                                               >  1:211232/1‑136 (MQ=255)                                                                       aTCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTTCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCTACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGacac                                                        <  4:258539/136‑1 (MQ=255)                                                                       aTCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGccac                                                        >  1:180898/1‑136 (MQ=255)                                                                       aTCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGccac                                                        >  3:199019/1‑136 (MQ=255)                                                                       aTCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGacac                                                        >  5:84166/1‑136 (MQ=255)                                                                       aTCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGacac                                                        >  7:234988/1‑136 (MQ=255)                                                                       aTCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGacac                                                        >  2:119116/1‑136 (MQ=255)                                                                       aTCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGacac                                                        >  2:349198/1‑136 (MQ=255)                                                                       aTCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGacac                                                        >  5:164164/1‑136 (MQ=255)                                                                       aTCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGacac                                                        >  5:154508/1‑136 (MQ=255)                                                                       aTCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACGTCACCTGCTCCGGCGCCCGCTGGCTCAGCAACGCCGTCTGCCGCGCAGCGGCCTTGGCcgcgcccc                                                        >  8:317222/1‑132 (MQ=255)                                                                               gCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGACACGGGTgg                                                  <  5:126505/134‑1 (MQ=255)                                                                               gCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGACACGGGTgg                                                  <  7:32972/134‑1 (MQ=255)                                                                               gCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGACACGGGTgg                                                  <  2:116334/134‑1 (MQ=255)                                                                                  aTTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTTCCGCGCATCGGCCTTGGCCTCGACACGGGTGGCGAAc                                             >  8:176696/1‑136 (MQ=255)                                                                                       cTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGACACGGGTGGCGAACGGCtt                                        >  6:329444/1‑136 (MQ=255)                                                                                          gggCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGACACGGGTGGCGAACGGCTTGc                                      <  3:352894/135‑1 (MQ=255)                                                                                             cGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGACACGGGTGGCGAACGGCTTGCCCAg                                  >  4:23784/1‑136 (MQ=255)                                                                                                    gAGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGACACGGGTGGCGAAAGGCTTGACCAGCGCCTCg                           >  6:184067/1‑136 (MQ=255)                                                                                                     aGGATCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGACACGGGTGGCGAACGGCTTGCCCAGCGCCTc                            <  3:61394/134‑1 (MQ=255)                                                                                                        aTCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGACCCGGGTGGCGAACGGCTTGCCCAGCGCCTCGGt                         >  1:249598/1‑134 (MQ=255)                                                                                                        aTCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGACACGGGTGGCGAACGGCTTGCCCAGCGCCTCGGt                         >  8:111572/1‑134 (MQ=255)                                                                                                        aTCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGACACGGGTGGCGAACGGCTTGCCCAGCGCCTCGGt                         >  3:125796/1‑134 (MQ=255)                                                                                                        aTCTGCACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGACACGGGTGGCGAACGGCTTGCCCAGCGCCGCGGt                         >  2:277281/1‑134 (MQ=255)                                                                                                             cACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGACACGGGTTTCGAACGGCTTGCCCAGCGCCTCGGTCAGGTc                   <  6:164164/135‑1 (MQ=255)                                                                                                             cACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGACACGGGTTGCGAACGGCTTGCCCAGCGCCTCGGTCa                       <  2:160916/131‑1 (MQ=255)                                                                                                             cACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGACACGGGTGGCGAACGGCTTGCCCAGCGCCTCGGTCa                       <  3:69323/131‑1 (MQ=255)                                                                                                             cACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGACACGGGTGGCGAACGGCTTGCCCAGCGCCTCGGTCa                       <  6:350226/131‑1 (MQ=255)                                                                                                             cACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGACACGGGTGGCGAACGGCTTGCCCAGCGCCTCGGTCa                       <  6:354877/131‑1 (MQ=255)                                                                                                             cACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGACACGGGTGGCGAACGGCTTGCCCAGCGCCTCGGTCa                       <  7:177240/131‑1 (MQ=255)                                                                                                             cACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGACACGGGTGGCGAACGGCTTGCCCAGCGCCTCGGTCAGGTCg                  <  5:3207/136‑1 (MQ=255)                                                                                                             cACGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGACACGGGTGGCGAACGGCTTGCCCAGCGCCTCGGTCAGGTCg                  <  6:240322/136‑1 (MQ=255)                                                                                                             cACGATCGGCTCACCGACCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGACACGGGTGGCGAACGGCTTGCCCAGCGCCTCGGTCAGGTCg                  <  3:219934/136‑1 (MQ=255)                                                                                                              aCGATCGGCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGACACGGGTGGCGAACGGCTTGCCCAGCGCCTCGGTCAGGTCgg                 >  5:148612/1‑136 (MQ=255)                                                                                                                  tCGGCGCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGACACGGGTGGCGAACGGCTTGCCCAGCGCCTCGGTCAGGTCGGCATa             <  8:99820/136‑1 (MQ=255)                                                                                                                     gCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGACACGGGTGGCGAACGGCTTGCCCAGCGCCTCGGTCAGGTCGGCATAAGc          >  2:98452/1‑136 (MQ=255)                                                                                                                     gCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGACACGGGTGGCGAACGGCTTGCCCAGCGCCTCGGTCAGGTCGGCATAAGc          >  5:235108/1‑136 (MQ=255)                                                                                                                     gCTCACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGACACGGGTGGCGAACGGCTTGCCCAGCGCCTCGGTCAGGTCGGAATAAGc          >  1:29251/1‑136 (MQ=255)                                                                                                                        cACCGGCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGACACGGGTGGCGAACGGCTTGCCCAGCGCCTCGGTCAGGGCGGCATAAGCCTg       <  2:195047/136‑1 (MQ=255)                                                                                                                          ccggccggCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCcgcg                                                                             <  6:92679/64‑1 (MQ=255)                                                                                                                          ccggccggCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCcgcg                                                                             >  5:92679/1‑64 (MQ=255)                                                                                                                             gCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGACACGGGTGGCGAACGGCTTGCCCAGCGCCTCGGTCAGGTCGGCATAAGCCTGGCTCg  <  6:240167/136‑1 (MQ=255)                                                                                                                             gCCGGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGACACGGGTGGCGAACGGCTTGCCCAGCGCCTCGGTCAGGTCGGCATAAGCCTGGCTCg  <  1:217717/136‑1 (MQ=255)                                                                                                                             gCCAGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGACACGGGTGGCGAACGGCTTGCCCAGCGCCTCGGTCAGGTCGGCATAAGCCTGGCTCg  <  3:47228/136‑1 (MQ=255)                                                                                                                                |                                                                                                                                     GTAGATGTCTTCAGTACCCGACGGGCGCGCGGCGAACCAGCCATTGGCCGTCATCACCTTAAGCCCGCCAATCGCCTGGCCATTGCCTGGGGCGTGGCTGAGGATCTGCACGATCGGCTCACCGGCCAGCTCTGTCGACTTCACCTGCTCCGGCGCCAGCTTGCTCAGCAACGCCTTCTGCCGCGCATCGGCCTTGGCCTCGACACGGGTGGCGAACGGCTTGCCCAGCGCCTCGGTCAGGTCGGCATAAGCCTGGCTCG  >  ACB143/4052979‑4053238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑