Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB143 4,190,676 G→A intergenic (+890/+101) CJ019_03940 → / ← CJ019_03941 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1434,190,6760GA97.8% 154.4 / NA 45intergenic (+890/+101)CJ019_03940/CJ019_03941hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (24/20);  minor base C (1/0);  total (25/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCGGATAGCTATCAGGGGCGATGCCCGAAGCCTAAAATTCAATGAAGAATGAAGGGACTTCCTTTCCTGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGGCAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTAAATACGCTAGCCAGCCAAACCACTTCTAGACT  >  ACB143/4190555‑4190802
                                                                                                                         |                                                                                                                              
aTCGGATAGCTATCAGGGGCGATGCCCGAAGCCTAAAATTCAATGAAGAATGAAGGGACTTCCTTTCCTGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTc                                                                                                                  >  5:342007/1‑136 (MQ=255)
   ggATAGCTATCAGGGGCGATGCCCGAAGCCTAAAATTCAATGAAGAATGAAGGGACTTCCTTTCCTGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGa                                                                                                                <  1:243983/135‑1 (MQ=255)
            tCAGGGGCGATGCCCGAAGCCTAAAATTCAATGAAGAATGAAGGGACTTCCTTTCCTGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGaa                                                                                                        <  1:12014/134‑1 (MQ=255)
               ggggCGATGCCCGAAGCCTAAAATTCAATGAAGAATGAAGGGACTTCCTTTCCTGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGACTCTGGAATACTc                                                                                                   <  2:330873/136‑1 (MQ=255)
                gggCGATGCCCGAAGCCTAAAATTCAATGAAGAATGAAGGGACTTCCTTTCCTGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTAGAATACTCg                                                                                                  <  6:118147/136‑1 (MQ=255)
                  gCGATGCCCGAAGCCTAAAATTCAATGAAGAATGAAGGGACTTCCTTTCCTGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAg                                                                                                >  6:183404/1‑136 (MQ=255)
                      tGCCCGAAGCCTAAAATTCAATGAAGAATGAAGGGACTTCCTTTCCTGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAg                                                                                             >  1:244437/1‑135 (MQ=255)
                      tGCCCGAAGCCTAAAATTCAATGAAGAATGAAGGGACTTCCTTTCCTGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAg                                                                                             >  6:109140/1‑135 (MQ=255)
                      tGCCCGAAGCCTAAAATTCAATGAAGAATGAAGGGACTTCCTTTCCTGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAg                                                                                             >  7:138120/1‑135 (MQ=255)
                       gCCCGAAGCCTAAAATTCAATGTAGAATGAAGGGACTTCCTTTCCTGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGaa                                                                                           >  2:250140/1‑136 (MQ=255)
                        cccGAAGCCTAAAATTCAATGAAGAATGAAGGGACTTCCTTTCCTGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAAt                                                                                          <  8:325834/136‑1 (MQ=255)
                         ccGAAGCCTAAAATTCAATGAAGAATGAAGGGACTTCCTTTCCTGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAAt                                                                                          >  4:254307/1‑135 (MQ=255)
                                                    aagGGACTTCCTTTCCTGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGc                                                              <  5:7231/136‑1 (MQ=255)
                                                         aCTTCCTTTCCTGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTcg                                                         <  5:335054/136‑1 (MQ=255)
                                                             cctttcctGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGAt                                                     >  8:340530/1‑136 (MQ=255)
                                                             cctttcctGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGAt                                                     >  8:67385/1‑136 (MQ=255)
                                                              ctttcctGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATc                                                    <  1:116533/136‑1 (MQ=255)
                                                                 tcctGTCCTAGGTCTACCGGATTCGTCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGa                                                  <  2:141916/135‑1 (MQ=255)
                                                                 tcctGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGa                                                  <  8:325775/135‑1 (MQ=255)
                                                                 tcctGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGa                                                  <  3:302403/135‑1 (MQ=255)
                                                                 tcctGTCCTAGGTCTACCGGATTCGGCCACACGATCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGa                                                  <  1:45858/135‑1 (MQ=255)
                                                                 tcctGTCCTAGGGCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCCCAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGa                                                  <  7:341547/135‑1 (MQ=255)
                                                                    tGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCg                                              >  7:292434/1‑136 (MQ=255)
                                                                        cTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTc                                           >  5:353431/1‑135 (MQ=255)
                                                                        cTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTc                                           >  5:359002/1‑135 (MQ=255)
                                                                              cTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTAc                                    >  2:277779/1‑136 (MQ=255)
                                                                              cTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAAGAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTAGTTAc                                    >  2:191784/1‑136 (MQ=255)
                                                                                 ccGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTAc                                    <  2:72776/133‑1 (MQ=255)
                                                                                 ccGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTAc                                    <  1:65773/133‑1 (MQ=255)
                                                                                   ggATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTaaa                                >  5:35079/1‑135 (MQ=255)
                                                                                        cGGCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTAAATACGc                           >  8:148973/1‑135 (MQ=255)
                                                                                          gCCACACGTTCCAAGATCGCCCCCTCCACAGCCAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTAAATACGCTag                        >  7:315502/1‑136 (MQ=255)
                                                                                          gCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTAAATACGCTag                        >  4:238507/1‑136 (MQ=255)
                                                                                          gCCACACGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTAAATACGCTag                        >  8:243615/1‑136 (MQ=255)
                                                                                            cacaCGTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTAAATACGCTagc                       >  5:177178/1‑135 (MQ=255)
                                                                                                 gTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTAAATACGCTagccagcc                  >  5:311503/1‑135 (MQ=255)
                                                                                                 gTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTAAATACGCTAgccagcca                 <  5:306733/136‑1 (MQ=255)
                                                                                                 gTTCCAAGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTAAATACGCTAgccagcca                 <  1:8435/136‑1 (MQ=255)
                                                                                                      aaGATCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTAAATACGCTAGCCAGCCAAACCa            >  2:274704/1‑136 (MQ=255)
                                                                                                         aTCGCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCTCCCTCGCGATCGAAGCGGTCTATTTACTAAATACGCTAGCCAGCCAAACCACtt         <  8:327420/136‑1 (MQ=255)
                                                                                                            gCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTAAATACGCTAGCCAGCCAAACCACTTCt       <  3:142963/135‑1 (MQ=255)
                                                                                                            gCCCCCTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTAAATACGCTAGCCAGCCAAACCACTTCt       <  1:338545/135‑1 (MQ=255)
                                                                                                                ccTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCTAGTAGAATAACAGATGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTAAATACGCTAGCCAGCCAAACCACTTCTAGACt  >  4:143987/1‑136 (MQ=255)
                                                                                                                ccTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTAAATACGCTAGCCAGCCAAACCACTTCTAGACt  >  2:141437/1‑136 (MQ=255)
                                                                                                                ccTCCACAGACAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTAAATACGCTAGCCAGCCAAACCACTTCTAGACt  >  8:87338/1‑136 (MQ=255)
                                                                                                                         |                                                                                                                              
ATCGGATAGCTATCAGGGGCGATGCCCGAAGCCTAAAATTCAATGAAGAATGAAGGGACTTCCTTTCCTGTCCTAGGTCTACCGGATTCGGCCACACGTTCCAAGATCGCCCCCTCCACAGGCAGTTCCAAGCTTCGAATCTGGAATACTCGAGTAGAATAACAGCTGCGAGGACGGCAAGCACCCGCCCTCGCGATCGAAGCGGTCTATTTACTAAATACGCTAGCCAGCCAAACCACTTCTAGACT  >  ACB143/4190555‑4190802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑