| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | ACB143 | 4,437,644 | C→T | D125N (GAC→AAC) | nicD ← | N‑formylmaleamate deformylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 4,437,644 | 0 | C | T | 97.2% | 114.1 / ‑4.0 | 36 | D125N (GAC→AAC) | nicD | N‑formylmaleamate deformylase |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (18/17); total (18/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-01 | |||||||||||
TCGCTCCAGGTGGCACAGAAGGCGCGCATGTCATCGCCGCTCATGCCAACCGTGGCTTGGCGGATCGAATCGACGTACCACGGCAACTTGCTCGGGTAGGCGCGGCGGCCCGGCCCGGACACCGGTGGGTCGACCAGCACCAGCCGCTGCAGTCCCGGTGCCCCTTGGGCGGCTGCGCGGATGGCAAAACGCGCCCCCATCGAGTGACCGAGCAGATGGTAGCTATCCAGGCCCAGCGCGGCGGCAAAGGCGGGAATATCCG > ACB143/4437514‑4437775 | tcgCTCCAGGTGGCACAGAAGGCGCGCAAGTCATCGCCGCTCATGCCAACCGGGGCTTGGCGGCGCGAATCGACGTACCACGGCAACTTGCTCGGGTCGGCGCGGCGGCCCGGCCCGGCCACCGGGGGGTTGac > 3:15801/1‑134 (MQ=255) cgCTCCAGGTGGCACAGAAGGCGCGCATGTCATCGCCGCTCATGCCAACCGTGGCTTGGCGGATCGAATCGACGTACCACGGCAACTTGCTCGGGTAGGCGCGGCGGCCCGGCCCGGACACCGGTGGGTTGaccag > 2:271957/1‑136 (MQ=255) cAGGTGGCACAGAAGGCGCGCATGTCATCGCCGCTCATGCCAACCGTGGCTTGGCGGATCGAATCGACGTACCACGGCAACTTGCTCGGGTAGGCGCGGCGGCCCGGCCCGGACACCGGTGGGTTGaccagcac > 2:2421/1‑134 (MQ=255) aGGTGGCACAGAAGGCGCGCATGTCATCGCCGCTCATGCCAACCGTGGCTTGGCGGATCGAATCGACGTACCACGGCAACTTGCTCGGGTAGGCGCGGCGGCCCGGCCCGGACACCGGTGGGTTGaccagcaccag > 4:171459/1‑136 (MQ=255) aGAAGGCGCGCATGTCATCGCCGCTCATGCCAACCGTGGCTTGGCGGATCGAATCGACGTACCACGGCAACTTGCTCGGGTAGGCGCGGCGGCCCGGCCCGGACACCGGTGGGTTGACCAGCACCAGCCGCTGCAg > 4:363030/1‑136 (MQ=255) aGGCGCGCATGTCATCGCCGCTCATGCCAACCGAGGCTTGGCGGATCGAATCGACGTACCACGGCAACTTGATCGGGTAGGCGCGGCGGCCCGGCCCGGACACCGGTGGGTTGACCAGCACCAGCCGCTGCAGTcc > 5:347336/1‑136 (MQ=255) ggCGCGCATGTCATCGCCGCTCATGCCAACCGTGGCTTGGCGGATCGAATCGACGTACCACGGCAACTTGCTCGGGTAGGCGCGGCGGCCCGGCCCGGACACCGGTGGGTTGACCAGCACCAGCCGCTGCAGTccc < 8:5209/136‑1 (MQ=255) ggCGCGCATGTCATCGCCGCTCATGCCAACCGTGGCTTGGCGGATCGAATCGACGTACCACGGCAACTTGCTCGGGTAGGCGCGGCGGCCCGGCCCGGACACCGGTGGGTTGACCAGCACCAGCCGCTGCAGTccc < 6:364821/136‑1 (MQ=255) gcgcATGTCATCGCCGCTCATGCCAACCGTGGCTTGGCGGATCGAATCGACGTACCACGGCAACTTGCTCGGGTAGGCGCGGCGGCCCGGCCCGGACACCGGTGGGTTGACCAGCACCAGCCGCTGCAGTCCCGGt > 5:110249/1‑136 (MQ=255) tCATCGCCGCTCATGCCAACCGTGGCTTGGCGGATCGAATCGACGTACCACGGCAACTTGCTCGGGTAGGCGCGGCGGCCCGGCCCGGACACCGGTGGGTTGACCAGCACCAGCCGCTGCAGTCCCGGTGCCCCtt < 6:363281/136‑1 (MQ=255) tCGCCGCTCATGCCAACCGTGGCTTGGCGGATCGAATCGACGTACCACGGCAACTTGCTCGGGTAGGCGCGGCGGCCCGGCCCGGACACCGGTGGGTTGACCAGCACCAGCCGCTGCAGTCCCGGTGCCCCTTggg < 2:353765/136‑1 (MQ=255) aTGCCAACCGTGGCTTGGCGGATCGAATCGACGTACCACGGCAACTTGCTCGGGTAGGCGCGGCGGCCCGGCCCGGCCACCGGTGGGTTGACCAGCACCAGCCGCTGCAGTCCCGGTGCCCCTTGGGCGGCTgcgc > 2:254237/1‑136 (MQ=255) aTGCCAACCGTGGCTTGGCGGATCGAATCGACGTACCACGGCAACTTGCTCGGGTAGGCGCGGCGGCCCGGCCCGGACACCGGTGGGTTGACCAGCACCAGCCGCTGCAGTCCCGGTGCCCCTTGGGCGGCTgcgc > 1:133429/1‑136 (MQ=255) aTGCCAACCGTGGCTTGGCGGATCGAATCGACGTACCACGGCAACTTGCTCGGGTAGGCGCGGCGGCCCGGCCCGGACACCGGTGGGTTGACCAGCACCAGCCGCTGCAGTCCCGGTGCCCCTTGGGCGGCTgcgc > 5:365008/1‑136 (MQ=255) aTGCCAACCGTGGCTTGGCGGATCGAATCGACGTACCACGGCAACTTGCTCGGGTAGGCGCGGCGGCCCGGCCCGGACACCGGTGGGTTGACCAGCACCAGCCGCTGCAGTCCCGGTGCCCCTTGGGCGGCTgcgc > 5:89693/1‑136 (MQ=255) aTGCCAACCGTGGCTTGGCGGATCGAAACGCCGTACCACGGCAACTTGCTCGGGTAGGCGCGGCGGCCCGGACCGGACACCGGTGGGTTGACCAGCACCAGCCGCTGCAGTCCCGGAGCCCCTTTGGCGGCTgcgc > 8:321204/1‑136 (MQ=255) gCTTGGCGGATCGAATCGACGTACCACGGCAACTTGCTCGGGTAGGCGCGGCGGCCCGGCCCGGACACCGGTGGGTTGACCAGCACCAGCCGCTGCAGTCCCGGTGCCCCTTGGGCGGCTGCGCGGATGGCAAAAc < 3:205899/136‑1 (MQ=255) gCTTGGCGGATCGAATCGACGTACCACGGCAACTTGCTCGGGTAGGCGCGGCGGCCCGGCCCGGACACCGGTGGGTTGACCAGCACCAGCCGCTGCAGTCCCGGTGCCCCTTGGGCGGCTGCGCGGATGGCAAAAc < 8:21198/136‑1 (MQ=255) ttGGCGGATCGAATCGACGTACCACGGCAACTTGCTCGGGTAGGCGCGGCGGCCCGGCCCGGACACCGGTGGGTTGACCAGCACCAGCCGCTGCAGTCCCGGTGCCCCTTGGGCGGCTGCGCGGATGGCAAAAcgc < 5:76766/136‑1 (MQ=255) ggCGGATCGAATCGACGTACCACGGCAACTTGCTCGGGTAGGCGCGGCGGCCCGGCCCGGACACCGGTGGGTTGACCAGCACCAGCCGCTGCAGTCCCGGTGCCCCTTGGGCGGCTGCGCGGATGGCAAAAcgc < 1:92998/134‑1 (MQ=255) cgaatcgaGGTACCACGGCAACTTGGTCGGGTAGGCGCGGCGGCCCGGCCCGGACACGGGTGGGTTGACCAGCACCAGCCGCTGCAGTCCCGGTGCCCCTTGGGCGGCTGGGGGGATGGCAAAACGCGCCCCCATc < 1:112292/136‑1 (MQ=255) aatcgaCGTACCACGGCAACTTGCTCGGGTAGGCGCGGCGGCCCGGCCCGGACACCGGTGGGTTGACCAGCACCAGCCGCTGCAGTCCCGGTGCCCCTTGGGCGGCTGCGCGGATGGCAAAACGCGCCCCCATc > 5:364402/1‑134 (MQ=255) cGTACCACGGCAACTTGCTCGGGTCGGCGCGGCGGCCCGGCCCGGACACCGGTGGGTTGACCAGCACCAGCCGCTGCAGTCCCGGTGCCCCTTGGGCGGCTGCGCGGATGGCAAAACGCGCCCCCATCGAGTGAcc < 4:29017/136‑1 (MQ=255) cGTACCACGGCAACTTGCTCGGGTAGGCGCGGGGGCCCGGCCCGGACACCGGTGGGTTGACCAGCACCAGCCGCTGCAGTCCCGGTGCCCCTTGGGCGGCTGCGCGGATGGCAAAACGCGCCCCCATCGAGTGAcc < 7:255436/136‑1 (MQ=255) cGTACCACGGCAACTTGCTCGGGTAGGCGCGGCGGCCCGGCCCGGACACCGGTGGGTTGACCAGCACCAGCCGCTGCAGTCCCGGTGCCCCTTGGGCGGCTGCGCGGATGGCAAAACGCGCCCCCATCGAGTGAcc < 4:370365/136‑1 (MQ=255) aaCCACGGCACCTTGCGCGGGAAGGCGCGGCGGCCCGGCCCGGACACCGGTGGGTCGACCAGCACCAGCCGCTGCAGTCCCGGTGCCCCTTGGGCGGCTGCGCGGATGGCAAAACGCGCCCCCATCGAGTGACCGa < 4:54087/135‑1 (MQ=255) aaCTTGCTCGGGTAGGCGCGGCGGCCCGGCCCGGACACCGGTGGGTTGACCAGCACCAGCCGCTGCAGTCCCGGTGCCCCTTGGGGGGCTGCGCGGATGGCAAAACGCGCCCCCATCGAGTGACCGAGCAGATGGt < 6:312257/136‑1 (MQ=255) tCGGGTAGGCGCGGCGGCCCGGCCCGGACACCGGTGGGTTGACCAGCACCAGCCGCTGCAGTCCCGGTGCCCCTTGGGCGGCTGCGCGGATGGCAAAACGCGCCCCCATCGAGTGACCGAGCAGATGGTAGCTATc > 2:23153/1‑136 (MQ=255) ggTAGGCGCGGCGGCCCGGCCCGGACACCGGTGGGTTGACCAGCACCAGCCGCTGCAGTCCCGGTGCCCCTTGGGCGGCTGCGCGGATGGCAAAACGCGCCCCCATCGAGTGACCGAGCAGATGGTAGCTATCCAg < 8:67523/136‑1 (MQ=255) gcccggcccgGACACCGGTGGGTTGACCAGCACCAGCCGCTGCAGTCCCGGTGCCCCTTGGGCGGCTGCGCGGATGGCAAAACGCGCCCCCATCGAGTGACCGAGCAGATGGTAGCTATCCAGGCCCAGCgcggcg > 7:214872/1‑136 (MQ=255) ggcccggCCACCGGGGGGTTGACCAGCACCAGCCGCTGCAGTCCCGGTGCCCCTTGGGCGGGTGCGCGGATGGCAACACGCGCCCCCATCGAGTGACCGAGCAGATGGTAGCTATCCAGGCCCAGCGCGGCGGCaa < 6:25191/136‑1 (MQ=255) ccGGTGGGTTGACCAGCACCAGCCGCTGCAGTCCCGGTGCCCCTTGGGCGGCTGCGCGGATGGCAAAACGCGCCCCCATCGAGTGACCGAGCAGATGGTAGCTATCCAGGCCCAGCGCGGCGGCAAAGGCGGGAat < 4:198044/136‑1 (MQ=255) ggTGGGTTGACCAGCACCAGCCGCTGCAGTCCCGGGGCCCCTTGGGCGGCTGCGCGGATGGCAAAACGCGCCCCCATCGAGTGACCGAGCAGATGGTAGCTATCCAGGCCCAGCGCGGCGGCAAAGGCGGGAatat < 1:365640/136‑1 (MQ=255) tGGGTTGACCAGCACCAGCCGCTGCAGTCCCGGTTCCACTTGGGCGGCTGCGCGGATGCCAAAACGCGCCCCCATCGAGTGACCGAGCAGCTGGTAGCTATACAGGCACAGCGCGGCGGCAAAGGCGGGAATATcc > 7:332326/1‑136 (MQ=255) tGGGTTGACCAGCACCAGCCGCTGCAGTCCCGGTGCCCCTTGGGCGGCTGCGCGGATGGCAAAACGCGCCCCCATCGAGTGACCGAGCAGATGGTAGCTATCCAGGCCCAGCGCGGCGGCAAAGGCGGGAATATcc > 2:39602/1‑136 (MQ=255) gggTTGACCAGCACCAGCCGCTGCAGTCCCGGTGCCCCTTGGGCGGCTGCGCGGATGGCAAAACGCGCCCCCATCGAGTGACCGAGCAGATGGTAGCTATCCAGGCCCAGCGCGGCGGCAAAGGCGGGAATATCCg > 1:18778/1‑136 (MQ=255) | TCGCTCCAGGTGGCACAGAAGGCGCGCATGTCATCGCCGCTCATGCCAACCGTGGCTTGGCGGATCGAATCGACGTACCACGGCAACTTGCTCGGGTAGGCGCGGCGGCCCGGCCCGGACACCGGTGGGTCGACCAGCACCAGCCGCTGCAGTCCCGGTGCCCCTTGGGCGGCTGCGCGGATGGCAAAACGCGCCCCCATCGAGTGACCGAGCAGATGGTAGCTATCCAGGCCCAGCGCGGCGGCAAAGGCGGGAATATCCG > ACB143/4437514‑4437775 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |