Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB143	4,749,727	C→T	S441S (AGC→AGT)	ttgI_4 →	Toluene efflux pump outer membrane protein TtgI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	4,749,727	0	C	T	100.0%	196.2 / NA	57	S441S (AGC→AGT)	ttgI_4	Toluene efflux pump outer membrane protein TtgI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/21);  total (36/21)

TGACCGTGCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAG  >  ACB143/4749600‑4749858                                                                                                                                |                                                                                                                                    tGACCGTGCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCAAACTGCAGGCCAGCGTGGCCCTGTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGAcgcg                                                                                                                             >  6:236894/1‑136 (MQ=255)   aCCGTGCTCGACAGCCAGGGCAGCCTGTACGAGGCCGAGGACCAGCAGGCCCGCCTGCAGGTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACACAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGAcgcg                                                                                                                             <  3:59555/134‑1 (MQ=255)         cTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGc                                                                                                                      <  7:80746/135‑1 (MQ=255)         cTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGc                                                                                                                      <  1:224832/135‑1 (MQ=255)         cTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCg                                                                                                                     >  3:87329/1‑136 (MQ=255)           cGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTACCCCAACTGCAGGCCAGCGTGGCCCTTTACACCGCCTTGGGCGGTGGCTGGCAAGCGGCTAGTCCGACGCGCTAGGGCGTc                                                                                                                   <  8:340963/136‑1 (MQ=255)            gACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTc                                                                                                                   <  7:245641/135‑1 (MQ=255)            gACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTc                                                                                                                   <  5:252446/135‑1 (MQ=255)                      gcAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTgg                                                                                                        >  7:46718/1‑136 (MQ=255)                        aGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCGTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGgtg                                                                                                      >  3:65481/1‑136 (MQ=255)                           cTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGtt                                                                                                    >  4:320736/1‑135 (MQ=255)                                 cAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGAcc                                                                                             >  4:165616/1‑136 (MQ=255)                                  aGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTaccgaccg                                                                                            >  1:319889/1‑136 (MQ=255)                                  aGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCg                                                                                            >  4:320592/1‑136 (MQ=255)                                    gCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCt                                                                                          <  3:285513/136‑1 (MQ=255)                                      ccAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGc                                                                                        >  2:152508/1‑136 (MQ=255)                                       cAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGTCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCGAGGGCGTCAGGCTATCTGGTGTTCCCGACCGCTGcc                                                                                       >  7:145325/1‑136 (MQ=255)                                       cAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGcc                                                                                       >  7:35297/1‑136 (MQ=255)                                       cAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGcc                                                                                       >  3:286279/1‑136 (MQ=255)                                       cAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTAGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGcc                                                                                       >  1:290966/1‑136 (MQ=255)                                           aCCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGCCCGCTGCCTGAc                                                                                   >  3:10529/1‑136 (MQ=255)                                                 agGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAg                                                                             <  2:305680/136‑1 (MQ=255)                                                  ggCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAgg                                                                            >  1:136848/1‑136 (MQ=255)                                                   gCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGt                                                                           <  6:59799/136‑1 (MQ=255)                                                   gCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGt                                                                           >  2:101517/1‑136 (MQ=255)                                                      cGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGc                                                                        <  8:35297/136‑1 (MQ=255)                                                      cGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGc                                                                        <  1:349405/136‑1 (MQ=255)                                                       gCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGc                                                                        >  8:82154/1‑135 (MQ=255)                                                           gCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCa                                                                   >  4:88024/1‑136 (MQ=255)                                                                tAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATAc                                                                >  3:189047/1‑134 (MQ=255)                                                                 aaGCCAACTGCAGGCCAGCGTGGCCCTTTACAACGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAg                                                             <  2:283177/136‑1 (MQ=255)                                                                     cAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGggc                                                         >  3:37940/1‑136 (MQ=255)                                                                     cAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGggc                                                         >  3:301108/1‑136 (MQ=255)                                                                      aaCTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGggcg                                                        >  7:48415/1‑136 (MQ=255)                                                                      aaCTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGggcg                                                        <  2:46423/136‑1 (MQ=255)                                                                      aaCTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTGCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGggcg                                                        <  1:158118/136‑1 (MQ=255)                                                                                 aGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACtt                                             >  1:124950/1‑136 (MQ=255)                                                                                 aGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACtt                                             >  5:60085/1‑136 (MQ=255)                                                                                 aGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACtt                                             >  7:65586/1‑136 (MQ=255)                                                                                 aGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACtt                                             >  7:279966/1‑136 (MQ=255)                                                                                     tGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGcc                                         <  4:28407/136‑1 (MQ=255)                                                                                           tttACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAg                                   >  2:47181/1‑136 (MQ=255)                                                                                                aaaGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCCCATTTCCTACCACGCCGt                              >  1:82008/1‑136 (MQ=255)                                                                                                aaaGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGt                              >  7:245959/1‑136 (MQ=255)                                                                                                aaaGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGt                              >  1:87582/1‑136 (MQ=255)                                                                                                aaaGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGt                              >  6:107169/1‑136 (MQ=255)                                                                                                aaaGCCTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGt                              >  5:220838/1‑136 (MQ=255)                                                                                                   gCCTTGGGCGGTGGCTGGCAAGCGTATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCa                            <  1:187197/135‑1 (MQ=255)                                                                                                     cTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAg                         >  7:184223/1‑136 (MQ=255)                                                                                                     cTTGGGCGGTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAg                         >  1:145958/1‑136 (MQ=255)                                                                                                            ggTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGctcct                  <  3:158715/136‑1 (MQ=255)                                                                                                            ggTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGctcct                  <  7:315571/136‑1 (MQ=255)                                                                                                            ggTGGCTGGCAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGctcct                  <  8:128455/136‑1 (MQ=255)                                                                                                                gctggcAAGCGGATAGTCCGACGCGCTAGGGCGTGAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCtcgt               <  3:326285/135‑1 (MQ=255)                                                                                                                 ctggcAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCtcgtcg             >  7:304208/1‑136 (MQ=255)                                                                                                                 ctggcAAGCGGATAGTCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCtcgtcg             >  7:113633/1‑136 (MQ=255)                                                                                                                            aTAGTCCGACGCGCTAGGCCGGCAGGCTATCTGGGGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAg  <  4:211597/136‑1 (MQ=255)                                                                                                                                |                                                                                                                                    TGACCGTGCTCGACACCCAGCGCAGCCTGTACCAGGCCCAGGACCAGCAGGCCCGCCTGCAGCTAAGCCAACTGCAGGCCAGCGTGGCCCTTTACAAAGCCTTGGGCGGTGGCTGGCAAGCGGATAGCCCGACGCGCTAGGGCGTCAGGCTATCTGGTGTTCACGACCGCTGCCTGACCTTCAGGTGGCGAGCATACCAGGGGCGGCGCGGCACTTTGCCTACCAGCCCGTCAAAGCGCTCCTCGTCGCTGTAAATCAG  >  ACB143/4749600‑4749858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑