Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB143 253,202 C→T G89G (GGC→GGT)  ssuC_1 → Putative aliphatic sulfonates transport permeaseprotein SsuC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB143253,2020CT97.5% 242.3 / NA 79G89G (GGC→GGT) ssuC_1Putative aliphatic sulfonates transport permeaseprotein SsuC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (42/35);  minor base G (0/2);  total (42/37)
Fisher's exact test for biased strand distribution p-value = 2.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGTGCTGGACGCTGCCTGGCAGTTGCTGACCTCCAGCACCCTGCTGGCGCACCTGGGCAGCAGCCTGGCGCGGGTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGCCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTCCCGTCGTCGGAACTGTCGATGGTGTTCATCACC  >  ACB143/253069‑253331
                                                                                                                                     |                                                                                                                                 
cGGTGCTGGACGCTGCCTGGCAGTTGCTGACCTCCAGCACCCTGCTGGCGCACCTGGGCAGCAGCCTGGCGCGGGTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCg                                                                                                                                 >  3:6341/1‑136 (MQ=255)
   tGCTGGACGCTGCCTGGCAGTTGCTGACCTCCAGCACCCTGCTGGCGCACCTGGGCAGCAGCCTGGCGCGGGTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTc                                                                                                                              <  2:229227/136‑1 (MQ=255)
     cTGGACGCTGCCTGGCAGTTGCTGACCTCCAGCACCCTGCTGGCGCACCTGGGCAGCAGCCTGGCGCGGGTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCg                                                                                                                             >  5:256424/1‑135 (MQ=255)
     cTGGACGCTGCCTGGCAGTTGCTGACCTCCAGCACCCTGCTGGCGCACCTGGGCAGCAGCCTGGCGCGGGTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCg                                                                                                                             >  3:259260/1‑135 (MQ=255)
     cTGGACGCTGCCTGGCAGTTGCTGACCTCCAGCACCCTGCTGGCGCACCTGGGCAGCAGCCTGGCGCGGGTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCg                                                                                                                             >  3:138913/1‑135 (MQ=255)
     cTGGACGCTGCCTGGCAGTTGCTGACCTCCAGCACCCTGCTGGCGCACCTGGGCAGCAGCCTGGCGCGGGTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCg                                                                                                                             >  7:251179/1‑135 (MQ=255)
      tGGACGCTGCCTGGCAGTTGCTGACCTCCAGCACCCTGCTGGCGCACCTGGGCAGCAGCCTGGCGCGGGTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGa                                                                                                                            >  2:16239/1‑135 (MQ=255)
      tGGACGCTGCCTGGCAGTTGCTGACCTCCAGCACCCTGCTGGCGCACCTGGGCAGCAGCCTGGCGCGGGTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCACTGGGGTTGCTGATCGGTCGCTCGa                                                                                                                            >  5:9125/1‑135 (MQ=255)
        gACGCTGCCTGGCAGTTGCTGACCTCCAGCACCCTGCTGGCGCACCTGGGCAGCAGCCTGGCGCGGGTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAAt                                                                                                                         <  6:31719/136‑1 (MQ=255)
        gACGCTGCCTGGCAGTTGCTGACCTCCAGCACCCTGCTGGCGCACCTGGGCAGCAGCCTGGCGCGGGTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAAt                                                                                                                         <  1:235257/136‑1 (MQ=255)
        gACGCTGCCTGGCAGTTGCTGACCTCCAGCACCCTGCTGGCGCACCTGGGCAGCAGCCTGGCGCGGGTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAAt                                                                                                                         <  1:235252/136‑1 (MQ=255)
        gACGCGGCCTGGCAGTTGCTGACCTCCAGCTCCCTGCTGGCGCACCTGGGCAGCAGCCTGGCGCGGGTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGGCGCTCGAAAt                                                                                                                         <  4:298645/136‑1 (MQ=255)
         aCGCTGCCTGGCAGTTGCTGACCTCCAGCACCCTGCTGGCGCACCTGGGCAGCAGCCTGGCGCGGGTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATg                                                                                                                        <  6:304280/136‑1 (MQ=255)
         aCGCTGCCTGGCAGTTGCTGACCTCCAGCACCCTGCTGGCGCACCTGGGCAGCAGCCTGGCGCGGGTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATg                                                                                                                        <  2:18570/136‑1 (MQ=255)
         aCGCTGCCTGGCAGTTGCTGACCTCCAGCACCCTGCTGGCGCACCTGGGCAGCAGCCTGGCGCGGGTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATg                                                                                                                        <  7:105880/136‑1 (MQ=255)
                cTGGCAGTTGCTGACCTCCAGCCCCCTGCTGGCGCACCTGGGCAGCAGCCTGGCGCGGGTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATGGGCTg                                                                                                                   >  5:365854/1‑134 (MQ=255)
                cTGGCAGTTGCTGACCTCCAGCACCCTGCTGGCGCACCTGGGCAGCAGCCTGGCGCGGGTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATGGGCTg                                                                                                                   >  8:251001/1‑134 (MQ=255)
                cTGGCAGTTGCTGACCTCCAGCACCCTGCTGGCGCACCTGGGCAGCAGCCTGGCGCGGGTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATGGGCTg                                                                                                                   >  8:255507/1‑134 (MQ=255)
                cTGGCAGTTGCTGACCTCCAGCACCCTGCTGGCGCACCTGGGCAGCAGCCTGGCGCGGGTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATGGGCTg                                                                                                                   >  6:180445/1‑134 (MQ=255)
                          cTGACCTCCAGCACCCTGCTGGCGCACCTGGGCAGCAGCCTGGCGCGGGTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATTGGCTGAAGATACCcagc                                                                                                       >  5:33535/1‑136 (MQ=255)
                           tGAGCTCCAGCTCCCTGGTGGCGGAGCTGGGGAGCACCCTGGCGCGGGTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCctgct                                                                                                      <  1:179594/136‑1 (MQ=255)
                            gACCTCCAGCACCCTGCTGGCGCACCTGGGCAGCAGCCTGGCGCGGGTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCctgctg                                                                                                     >  3:119442/1‑136 (MQ=255)
                            gACCTCCAGCACCCTGCTGGCGCACCTGGGCAGCAGCCTGGCGCGGGTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCctgctg                                                                                                     >  2:153705/1‑136 (MQ=255)
                                  cAGCACCCTGCTGGCGCACCTGGGCAGCAGCCTGGCGCGGGTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGccgccg                                                                                               >  5:167578/1‑136 (MQ=255)
                                    gCACCCTGCTGGCGCACCTGGGCAGCAGCCTGGCGCGGGTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCt                                                                                             >  3:326585/1‑136 (MQ=255)
                                     cACGCTGCTGGCGCACCTGTGCATCAGCCTGGCGCGGGTATTTGCCGGTTATGCGACTGCGGCACTGTTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATGGTCTGAAGATACCCTGCTGCCGCCGCTg                                                                                            <  8:337185/136‑1 (MQ=255)
                                     cACCCTGCTGGCGCACCTGGGCAGCAGCCTGGCGCGGGTATTTGCCGGTTATGCCCCTGCGGCCCTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCcgccgcgg                                                                                            >  6:157527/1‑134 (MQ=255)
                                             tgGCGCACCTGGGCAGCAGCCTGGCGCGGGTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGtgct                                                                                    >  1:183536/1‑136 (MQ=255)
                                             tgGCGCACCTGGGCAGCAGCCTGGCGCGGGTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGtgct                                                                                    >  3:195169/1‑136 (MQ=255)
                                                     cTGGGCAGCAGCCTGGCGCGGGTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGa                                                                            <  8:228252/136‑1 (MQ=255)
                                                     cTGGGCAGCAGCCTGGCGCGGGTATTTGCCGGTTATGCCACTGAGGCACTGGTTGGCGTGGCCCTGTGGTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGa                                                                            >  6:479/1‑136 (MQ=255)
                                                       gggCAGCAGCCTGGCGCGGGTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATg                                                                                                                        <  1:373346/90‑1 (MQ=255)
                                                       gggCAGCAGCCTGGCGCGGGTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATg                                                                                                                        >  2:373346/1‑90 (MQ=255)
                                                       gggCAGCAGCCTGGCGCGGGTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGAtt                                                                          >  2:280102/1‑136 (MQ=255)
                                                       gggCAGCAGCCTGGCGCGGGTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGAtt                                                                          >  6:285229/1‑136 (MQ=255)
                                                       gggCAGCAGCCTGGCGCGGGTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGAtt                                                                          >  8:192052/1‑136 (MQ=255)
                                                        ggCATCAGCCTGGCGCGGGTATTTGCCGGTTATGCCACTTCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAACTGGGCTTAAGATAGCCTGCTGCCGCCGATGGAAGTGCTTCGGCCGATTc                                                                         <  3:85422/136‑1 (MQ=255)
                                                          gagcagcCTGGCGCGGGTATTTGCCGGTTATGACACTGCGGCACTGGTTGGCGTGGCCCTGGGGGTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCg                                                                       <  7:39134/135‑1 (MQ=255)
                                                          cagcagCCTGGCGCGGGTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCg                                                                       <  7:192052/136‑1 (MQ=255)
                                                           agcagcCTGGCGCGGGTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGGTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCgg                                                                      <  6:266923/136‑1 (MQ=255)
                                                           agcagcCTGGCGCGGGTATTTGCCGGTTATGCCACTGCGGCACTGGGTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCgg                                                                      <  5:282815/136‑1 (MQ=255)
                                                            gcagcCTGGCGCGGGTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGc                                                                     >  4:165413/1‑136 (MQ=255)
                                                                ccTGGCGCGGGTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCtgtg                                                                 >  2:62306/1‑136 (MQ=255)
                                                                       cgGGTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGa                                                          >  2:99646/1‑136 (MQ=255)
                                                                        gggTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGAt                                                         >  3:313112/1‑136 (MQ=255)
                                                                          gTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGCTTCCGGCTGTGGCGTGGATcc                                                       >  1:139713/1‑136 (MQ=255)
                                                                             tttGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGc                                                    <  3:277175/136‑1 (MQ=255)
                                                                             tttGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGc                                                    <  5:117137/136‑1 (MQ=255)
                                                                             tttGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGc                                                    <  4:50744/136‑1 (MQ=255)
                                                                             tttGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGc                                                    <  4:342222/136‑1 (MQ=255)
                                                                             tttGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGc                                                    <  3:351989/136‑1 (MQ=255)
                                                                              ttggaggTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCGTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGc                                                    <  4:193243/130‑1 (MQ=255)
                                                                              ttGCCGGTTATGCCCCTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGATGATCGGTCGCTCTAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGc                                                    <  4:264807/135‑1 (MQ=255)
                                                                                 ccGGTTATGCCACTGCGGCACTGGTTGGCGTGGCGCTGGGGTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTgg                                                 <  1:356345/135‑1 (MQ=255)
                                                                                             cttcGGCACTGGTTGGCGTGGCCCGGGGGTTGCTGATCGGTCGCTCGAAATGGGCTTAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGtt                                    <  7:199495/133‑1 (MQ=255)
                                                                                                 ggCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTCCcg                                >  3:61848/1‑136 (MQ=255)
                                                                                                 ggCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTCCcg                                >  3:20342/1‑136 (MQ=255)
                                                                                                  gCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTCCcgt                               <  1:224041/136‑1 (MQ=255)
                                                                                                           ggCGTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTACCGTCGTCGGAAc                      >  6:32045/1‑136 (MQ=255)
                                                                                                           ggCGTGGCCCTGGGGTTGCTGATCGGGCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTCCCGTCGTCGGAAc                      <  2:357941/136‑1 (MQ=255)
                                                                                                              gTGGCCCTGGGGTTGCTGTTCGGTCGCTCGAAATGGGTTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTCCCGTCGTCGGAACTGt                   <  6:33535/136‑1 (MQ=255)
                                                                                                              gTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTCCCGTCGTCGGAACTGt                   <  7:153027/136‑1 (MQ=255)
                                                                                                              gTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTCCCGTCGTCGGAACTGt                   <  7:207156/136‑1 (MQ=255)
                                                                                                              gTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTCCCGTCGTCGGAACTGt                   <  7:9548/136‑1 (MQ=255)
                                                                                                              gTGGCCCTGGGGTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTCCCGTCGTCGGAACTGt                   <  8:150550/136‑1 (MQ=255)
                                                                                                                     tGGGGTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTCCCGTCGTCGGAACTGTCGATGgt            >  6:216483/1‑136 (MQ=255)
                                                                                                                     tGGGGTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTCCCGTCGTCGGAACTGTCGAGGgt            >  2:190140/1‑136 (MQ=255)
                                                                                                                       gggTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTCCCGTCGTCGGAACTGTCGATGgtgt          <  3:198723/136‑1 (MQ=255)
                                                                                                                       gggTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTCCCGTCGTCGGAACTGTCGATGgtgt          <  4:6341/136‑1 (MQ=255)
                                                                                                                       gggTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTCCCGTCGTCGGAACTGTCGATGgtgt          <  3:289392/136‑1 (MQ=255)
                                                                                                                       gggTTGCTGATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTCCCGTCGTCGGAACTGTCGATGgtgt          <  8:119612/136‑1 (MQ=255)
                                                                                                                               gATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTCCCGTCGTCGGAACTGTCGATGGTGTtcatccc   >  1:172809/1‑133 (MQ=255)
                                                                                                                               gATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTCCCGTCGTCGGAACTGTCGATGGTGTTGATAAc   >  2:98726/1‑135 (MQ=255)
                                                                                                                               gATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTCCCGTCGTCGGAACTGTCGATGGTGTTCATcccc  >  1:79305/1‑136 (MQ=255)
                                                                                                                               gATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTCCCGTCGTCGGAACTGTCGATGGTGTTCATCAcc  >  8:230561/1‑136 (MQ=255)
                                                                                                                               gATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTCCCGTCGTCGGAACTGTCGATGGTGTTCATCAcc  >  5:256081/1‑136 (MQ=255)
                                                                                                                               gATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTCCCGTCGTCGGAACTGTCGATGGTGTTCATCAcc  >  8:6216/1‑136 (MQ=255)
                                                                                                                               gATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTCCCGTCGTCGGAACTGTAGATGGTGTtaatccc   >  5:32188/1‑133 (MQ=255)
                                                                                                                               gATCGGTCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCAGTGGCGTGGATCCCGCTGGCGAACCTGATGTTCCCGTCGGCGGAACTGGGGATGGTGTtcaaacc   >  5:30030/1‑131 (MQ=255)
                                                                                                                                     |                                                                                                                                 
CGGTGCTGGACGCTGCCTGGCAGTTGCTGACCTCCAGCACCCTGCTGGCGCACCTGGGCAGCAGCCTGGCGCGGGTATTTGCCGGTTATGCCACTGCGGCACTGGTTGGCGTGGCCCTGGGGTTGCTGATCGGCCGCTCGAAATGGGCTGAAGATACCCTGCTGCCGCCGCTGGAAGTGCTGCGGCCGATTCCGGCTGTGGCGTGGATCCCGCTGGCGATCCTGATGTTCCCGTCGTCGGAACTGTCGATGGTGTTCATCACC  >  ACB143/253069‑253331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑