Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB143 5,050,496 G→A G225G (GGC→GGT hcnB ← Hydrogen cyanide synthase subunit HcnB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,050,4960GA98.6% 226.9 / ‑4.7 73G225G (GGC→GGThcnBHydrogen cyanide synthase subunit HcnB
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (53/19);  total (54/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

ATAGAAGGAATCACCCCTTCGTGGGACAGTAGGACGCTCGCCGGCACTGTGCAGAGCTCCCCGCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACGCCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGACTTCCTTGAACTCAGCAACCATCGAGCTCGCCCATGGAAGCGCGCGTCGCCAACCGCCTGGCGGCGTA  >  ACB143/5050371‑5050625
                                                                                                                             |                                                                                                                                 
aTAGAAGGAATCACCCCTTCGTGGGACAGTAGGACGCTCGCCGGCACTGTGCAGAGCTCCCCGCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTc                                                                                                                         <  4:208610/136‑1 (MQ=255)
 tAGAAGGAATCACCCCTTCGTGGGACAGTAGGACGCTCGCCGGCACTGTGCAGAGCTCCCCGCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCg                                                                                                                        <  8:151178/136‑1 (MQ=255)
   gAAGGAATCACCCCTTCGTGGGACAGTAGGACGCTCGCCGGCACTGTGCAGAGCTCCCCGCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCgcc                                                                                                                      >  4:234350/1‑134 (MQ=255)
   gAAGGAATCACCCCTTCGTGGGACAGTAGGACGCTCGCCGGCACTGTGCAGAGCTCCCCGCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGAc                                                                                                                      >  8:251496/1‑136 (MQ=255)
            aCCCCTTCGTGGGACAGTAGGACGCTCGCCGGCACTGTGCAGAGCTCCCCGCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACGCCTTTGATTCGACGGGCTCCCg                                                                                                             >  2:90622/1‑136 (MQ=255)
            aCCCCTTCGTGGGACAGTAGGACGCTCGCCGGCACTGTGCAGAGCTCCCCGCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTccc                                                                                                              >  6:176915/1‑135 (MQ=255)
            aCCCCTTCGTGGGACAGTAGGACGCTCGCCGGCACTGTGCAGAGCTCCCCGCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTccc                                                                                                              >  3:369881/1‑135 (MQ=255)
            aCCCCTTCGTGGGACAGTAGGACGCTCGCCGGCACTGTGCAGAGCTCCCCGCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTccc                                                                                                              >  3:98116/1‑135 (MQ=255)
            aCCCCTTCGTGGGACAGTAGGACGCTCGCCGGCACTGTGCAGAGCTCCCCGCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTccc                                                                                                              >  4:156976/1‑135 (MQ=255)
            aCCCCTTCGTGGGACAGTAGGACGCTCGCCGGCACTGTGCAGAGCTCCCCGCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCg                                                                                                             >  7:7092/1‑136 (MQ=255)
            aCCCCTTCGTGGGACAGTAGGACGCTCGCCGGCACAGTGCAGAGCTCCCCGCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCATGGCCCGCAGCTCTTTAACACCTTTGATACGACGGGCTccc                                                                                                              >  3:306154/1‑135 (MQ=255)
               ccTTCGTGGGACAGTAGGACGCTCGCCGGCACTGTGCAGAGCTCCCCGCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGc                                                                                                          <  3:179569/136‑1 (MQ=255)
                 ttCGTGGGACAGTAGGACGCTCGCCGGCACTGTGCAGAGCTCCCCGCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGa                                                                                                        >  6:103324/1‑136 (MQ=255)
                         aCAGTAGGACGCTCGCCGGCACTGTGCAGAGCTCCCCGCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTc                                                                                                <  2:200979/136‑1 (MQ=255)
                         aCAGTAGGACGCTCGCCGGCACTGTGCAGAGCTCCCCGCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTc                                                                                                <  2:217880/136‑1 (MQ=255)
                         aCAGTAGGACGCTCGCCGGCACTGTGCAGAGCTCCCCGCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTc                                                                                                <  5:176915/136‑1 (MQ=255)
                         aCAGTAGGACGCTCGCCGGCACTGTGCAGAGCTCCCCGCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTc                                                                                                <  1:162143/136‑1 (MQ=255)
                                gACGCTCGCCGGCACTGTGCAGAGCTCCCCGCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGc                                                                                         >  3:191382/1‑136 (MQ=255)
                                    cTCGCCGGCACTGTGCAGAGCTCCCCGCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATg                                                                                     <  5:59718/136‑1 (MQ=255)
                                     tCGCCGGCACTGTGCAGAGCTCCCCGCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCCTg                                                                                     >  1:192697/1‑135 (MQ=255)
                                     tCGCCGGCACTGTGCAGAGCTCCCCGCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATg                                                                                     >  2:128152/1‑135 (MQ=255)
                                       gCCGGCACTGTGCAGAGCTCCCCGCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGc                                                                                    >  1:10895/1‑134 (MQ=255)
                                          ggCACTGTGCAGAGCTCCCCGCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGc                                                                               >  6:90378/1‑136 (MQ=255)
                                          ggCACTGTGCAGAGCTCCCCGCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGc                                                                               >  3:298774/1‑136 (MQ=255)
                                                 tgCAGAGCTCCCCGCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTAc                                                                         <  8:205775/135‑1 (MQ=255)
                                                  gCAGAGCTCCCCGCTTTTGAGCCTGTATTGAAGCTGCTCAAGCCGTCCATCACCCAGGGCCCGCAGCTCTTTCACACGTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTg                                                                       <  2:250169/136‑1 (MQ=255)
                                                   ctgagCTCCCCGCTTTTGAGCCTGAATTGAAGCTTCTCACGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTg                                                                       <  3:309979/133‑1 (MQ=255)
                                                    agagCTCCCCGCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCCCCCAGGGCCCGCAGCTCTGTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGAc                                                                     >  4:219505/1‑136 (MQ=255)
                                                    agagCTCCCCGCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGAc                                                                     >  4:151604/1‑136 (MQ=255)
                                                    agagCTCCCCGCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGAc                                                                     >  5:116145/1‑136 (MQ=255)
                                                    agagCTCCCCGCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGAc                                                                     >  5:60374/1‑136 (MQ=255)
                                                    agagCTCCCCGCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGAc                                                                     >  1:76596/1‑136 (MQ=255)
                                                    agagCTCCCCGCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGAc                                                                     >  2:2654/1‑136 (MQ=255)
                                                    agagCTCCCCGCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGAc                                                                     >  2:77115/1‑136 (MQ=255)
                                                    agagCTCCCCGCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGAc                                                                     >  2:99780/1‑136 (MQ=255)
                                                    agagCTCCCCGCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGAc                                                                     >  3:310467/1‑136 (MQ=255)
                                                    agagCTCCCCGCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGAc                                                                     >  6:62385/1‑136 (MQ=255)
                                                           cccGCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGACTTCCtt                                                               >  1:364009/1‑135 (MQ=255)
                                                           cccGCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTCTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGACTTCCtt                                                               >  6:276150/1‑135 (MQ=255)
                                                             cGCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGACTTCCTTGaa                                                            <  3:81733/136‑1 (MQ=255)
                                                              gCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCCCCCAGCGCCCGCAGCACTTTCACACCTTTGATTAGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGACTTCCTTGAAc                                                           >  5:239221/1‑136 (MQ=255)
                                                              gCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTTATTCGACGGGCTCCCGAGCGACCGATTTCATCAAGCCAAGAATGGCCCTTACTCACTTCCTTGaa                                                            >  5:273159/1‑135 (MQ=255)
                                                              gCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGACTTCCTTGAAc                                                           >  5:327621/1‑136 (MQ=255)
                                                              gCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGACTTCCTTGAAc                                                           >  3:199066/1‑136 (MQ=255)
                                                              gCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGACTTCCTTGAAc                                                           >  1:227910/1‑136 (MQ=255)
                                                              gCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGACTTCCTTGAAc                                                           >  6:175843/1‑136 (MQ=255)
                                                              gCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGACTTCCTTGAAc                                                           >  1:131129/1‑136 (MQ=255)
                                                              gCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGACTTCCTTGAAc                                                           >  7:352416/1‑136 (MQ=255)
                                                              gCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGACTTCCTTGAAc                                                           >  2:122832/1‑136 (MQ=255)
                                                              gCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGACTTCCTTGAAc                                                           >  4:163808/1‑136 (MQ=255)
                                                              gCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGACTTCCTTGAAc                                                           >  3:292456/1‑136 (MQ=255)
                                                              gCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGACTTCCTTGAAc                                                           >  3:280049/1‑136 (MQ=255)
                                                              gCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTAATGCAGCCATGCAAGGCCCTTACTGACTTCCTTGAAc                                                           >  5:83824/1‑136 (MQ=255)
                                                              gCTTTTGAGACTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTATCACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGACTTCCTTGagc                                                           >  5:293280/1‑134 (MQ=255)
                                                              gCTGTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCGGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGCGTTGCTTGatc                                                           >  7:96392/1‑134 (MQ=255)
                                                                ttttGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGACTTCCTTGAACt                                                          >  5:285010/1‑135 (MQ=255)
                                                                  ttGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGACTTCCTTGAACTCa                                                        <  4:89821/135‑1 (MQ=255)
                                                                        cTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGACTTCCTTGAACTCAGCAACCa                                                 >  5:331373/1‑136 (MQ=255)
                                                                        cTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGACTTCCTTGAACTCAGCAACCa                                                 >  1:174177/1‑136 (MQ=255)
                                                                           aaTTGCAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGACTTCCTTGAACTCAGCAACCATc                                               <  5:48401/135‑1 (MQ=255)
                                                                           aaTTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGACTTCCTTGAACTCAGCAACCATCg                                              <  3:174274/136‑1 (MQ=255)
                                                                            aTTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACTGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGACTTCCTTGAACTCAGCAACCATCg                                              <  4:98116/135‑1 (MQ=255)
                                                                             ttGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGACTTCCTTGAACTCAGCAACCATCGAg                                            <  8:7092/136‑1 (MQ=255)
                                                                                                 cATCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGACTTCCTTGAACTCAGCAACCATCGAGCTCGCCCATGGAAGcgcgcg                        >  8:220281/1‑136 (MQ=255)
                                                                                                   tCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGACTTCCTTGAACTCAGCAACCATCGCGCTCGCCCATGGAAGCGCGCGTc                      >  4:61970/1‑136 (MQ=255)
                                                                                                   tCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGACTTCCTTGAACTCAGCAACCATCGAGCTCGCCCATGGAAGCGCGCGTc                      >  3:86130/1‑136 (MQ=255)
                                                                                                   tCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGACTTCCTTGAACTCAGCAACCATCGAGCTCGCCCATGGAAGCGCGCGTc                      >  3:232373/1‑136 (MQ=255)
                                                                                                   tCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGACTTCCTTGAACTCAGCAACCATCGAGCTCGCCCATGGAAGCGCGCGTc                      >  8:49875/1‑136 (MQ=255)
                                                                                                   tCACCCAGGGCCCGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGACTTCATTGAACTCAGCAACCATCGAGCTCGACCATGGAAGCGCGCGTc                      >  8:218215/1‑136 (MQ=255)
                                                                                                              ccGCAGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGACTTCCTTGAACTCAGCAACCATCGAGCTCGCCCATGGAAGCGCGCGTCGCCAACCGCCt           <  1:122832/136‑1 (MQ=255)
                                                                                                                  aGCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGACTTCCTTGAACTCAGCAACCATCGAGCTCGCCCATGGAAGCGCGCGTCGCCAACCGCCTggcg       >  4:325002/1‑136 (MQ=255)
                                                                                                                   gCTCTTTCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGACTTCCTTGAACTCAGCAACCATCGAGCTCGCCCATGGAAGCGCGCGTCGCCAACCGCCTggcgg      >  8:311136/1‑136 (MQ=255)
                                                                                                                         tCACACCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGACTTCCTTGAACTCAGCAACCATCGAGCTCGCCCATGGAAGCGCGCGTCGCCAACCGCCTGGCGGCgta  <  8:152552/134‑1 (MQ=255)
                                                                                                                             |                                                                                                                                 
ATAGAAGGAATCACCCCTTCGTGGGACAGTAGGACGCTCGCCGGCACTGTGCAGAGCTCCCCGCTTTTGAGCCTGAATTGAAGCTGCTCAAGCCGGCCATCACCCAGGGCCCGCAGCTCTTTCACGCCTTTGATTCGACGGGCTCCCGAGCGACGGATTTCATGCAGCCATGCAAGGCCCTTACTGACTTCCTTGAACTCAGCAACCATCGAGCTCGCCCATGGAAGCGCGCGTCGCCAACCGCCTGGCGGCGTA  >  ACB143/5050371‑5050625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: