Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB143	5,345,605	G→A	Q611* (CAG→TAG)	pnp ←	Polyribonucleotide nucleotidyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	5,345,605	0	G	A	100.0%	92.4 / NA	27	Q611* (CAG→TAG)	pnp	Polyribonucleotide nucleotidyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/12);  total (15/12)

GCATGGAGATGTGCACCAAACCGTCCTTGCCAGGCAGGATGTTGACGAAGGCGCCGAAGTCGACAATGCGCTCAACCTTGCCGACGTAGATCTTGCCGATCTCGGCCTCAGCGGTGATGCCCAGGATGCGCTGCTTGGCAGCATCTGCCGCTTCCTTGGTTTCGCCGAAGATCTTGATCGAGCCGTCGTCTTCGATGTCGATCGAAGCCTTGGTCTCTTCGCAGATGGCGCGAATGGTGGCGCCGCCTTTACCGATGACG  >  ACB143/5345473‑5345732                                                                                                                                     |                                                                                                                                gCATGGAGATGTGCACCAAACCGTCCTTGCCAGGCAGGATGTTGACGAAGGCGCCGAAGTCGACAATGCGCTCAACCTTGCCGACGTAGATCTTGCCGATCTCGGCCTCAGCGGTGATGCCCAGGATGCGCTACtt                                                                                                                              <  2:48319/136‑1 (MQ=255)               aCCAAACCGTCCTTGCCAGGCAGGATGTTGACGAAGGCGCCGAAGTCGACAATGCGCTCAACCTTGCCGACGTAGATCTTGCCGATCTCGGCCTCAGCGGTGATGCCCAGGATGCGCTACTTGGCAGCATCTGCCg                                                                                                                >  8:334108/1‑136 (MQ=255)               aCCAAACCGTCCTTGCCAGGCAGGATGTTGACGAAGGCGCCGAAGTCGACAATGCGCTCAACCTTGCCGACGTAGATCTTGCCGATCTCGGCCTCAGCGGTGATGCCCAGGATGCGCTACTTGGCAGCATCTGCCg                                                                                                                >  6:170095/1‑136 (MQ=255)               aCCAAACCGTCCTTGCCAGGCAGGATGTTGACGAAGGCGCCGAAGTCGACAATGCGCTCAACCTTGCCGACGTAGATCTTGCCGATCTCGGCCTCAGCGGTGATGCCCAGGATGCGCTACTTGGCAGCATCTGCCg                                                                                                                >  3:273752/1‑136 (MQ=255)               aCCAAACCGTCCTTGCCAGGCAGGATGTTGACGAAGGCGCCGAAGTCGACAATGCGCTCAACCTTGCCGACGTAGATCTTGCCGATCTCGGCCTCAGCGGTGATGCCCAGGATGCGCTACTTGGCAGCATCTGCCg                                                                                                                >  3:26770/1‑136 (MQ=255)               aCCAAACCGTCCTTGCCAGGCAGGATGTTGACGAAGGCGCCGAAGTCGACAATGCGCTCAACCTTGCCGACGTAGATCTTGCCGATCTCGGCCTCAGAGGTGATGCCCAGGATGCGCTACTTGGCAGCATCTGcag                                                                                                                >  4:8647/1‑134 (MQ=255)               aCCAAACCGTCCTTGCCAGGCAGGATGTTGACGAAGGCGCCGAAGTAGACAATGCGCTCAACCTTGACGACGTAGATCTTGCCGATCTCGGCCTCAGCGGTGATGCCCAGGATGCGCTACTTGGAAGCATCTGCCg                                                                                                                >  3:39853/1‑136 (MQ=255)                    aCCGTCCTTGCCAGGCAGGATGTTGACGAAGGCGCCGAATTCGACAATTCGCTCAACCTTGCCGACGTAGATCTTGCCGATCTCGGCCTCAGCGGTGATTCCCAGGATGCTCTAATTGGCAGCATCTGCCGCttaa                                                                                                           >  7:271467/1‑134 (MQ=255)                              ccAGGCAGGATGTTGACGAAGGCGCCGAAGTCGACGATGCGCTCAACCTTGCCGACGTAGATCTTGCCGATCTCGGCCTCAGCGGTGATGCCCAGGATGCGCTACTTGGCAGCATCTGCCGCTTCCTTGGTTTCg                                                                                                  <  6:41433/135‑1 (MQ=255)                              ccAGGCAGGATGTTGACGAAGGCGCCGAAGTCGACAATGCGCTCAACCTTGCCGACGTAGATCTTGCCGATCTCGGCCTCAGCGGTGATGCCCAGGATGCGCTACTTGGCAGCATCTGCCGCTTCCTTGGTTTCg                                                                                                  <  7:266512/135‑1 (MQ=255)                                aggcaggATGTTGACGAAGGCGCCGAAGTCGACAATGCGCTCAACCTTGCCGACGTAGATCTTGCCGATCTCGGCCTCAGCGGTGATGCCCAGGATGCGCTACTTGGCAGCATCTGCCGCTTCCTTGGTTTCGcc                                                                                                >  4:8080/1‑135 (MQ=255)                                aggcaggATGTTGACGAAGGCGCCGAAGTCGACAATGCGCTCAACCTTGCCGACGTAGATCTTGCCGATCTCGGCCTCAGCGGTGATGCCCAGGATGCGCTACTTGGCAGCATCTGCCGCTTCCTTGGTTTCGcc                                                                                                >  5:135605/1‑135 (MQ=255)                                     ggATGTTGACGAAGGCGCCGAAGTCGACAATGCGCTCAACCTTGCCGACGTAGATCTTGCCGATCTCGGCCTCAGCGGTGATGCCCAGGATGCGCTACTTGGCAGCATCTGCCGCTTCCTTGGTTTCGCCGAAGa                                                                                           >  6:229494/1‑135 (MQ=255)                                             aCGAAGGCGCCGAAGTCGACAATGCGCTCAACCTTGCCGACGTAGATCTTGCCGATCTCGGCCTCAGCGGTGATGCCCAGGATGCGCTACTTGGCAGCATCTGCCGCTTCCTTGGTTTCGCCGAAGATCTTGATc                                                                                   <  2:137388/135‑1 (MQ=255)                                               gAAGGCGCCGAAGTCGACAATGCGCTCAACCTTGCCGACGTAGATCTTGCCGATCTCGGCCTCAGCGGTGATGCCCAGGATGCGCTACTTGGCAGCATCTGCCGCTTCCTTGGTTTCGCCGAAGATCTTGATCGAg                                                                                <  7:5347/136‑1 (MQ=255)                                                                       cTCAACCTTGCCGACGTAGATCTTGCCGATCTCGGCCTCAGCGGTGATGCCCAGGATGCGCTACTTGGCAGCATCTGCCGCTTCCTTGGTTTCGCCGAAGATCTTGATCGAGCCGTCGTCTTCGATGTCGATCGaa                                                        <  6:295883/136‑1 (MQ=255)                                                                        tCAACCTTGCCGACGTAGATCTTGCCGATCTCGGCCTCAGCGGTGATGCCCAGGATGCGCTACTTGGCAGCATCTGCCGCTTCCTTGGTTTCGCCGAAGATCTTGATCGAGCCGTCGTCTTCGATGTCGATCGAAg                                                       >  5:151166/1‑136 (MQ=255)                                                                            ccTTGCCGACGTAGATCTTGCCGATCTCGGCCTCAGCGGTGATGCCCAGGATGCGCTACTTGGCAGCATCTGCCGCTTCCTTGGTTTCGCCGAAGATCTTGATCGAGCCGTCGTCTTCGATGTCGATCGAAGCCtt                                                   <  5:123224/136‑1 (MQ=255)                                                                                       tAGATCTTGCCGATCTCGGCCTCAGCGGTGATGCCCAGGATGCGCTACTTGGCAGCATCTGCCGCTTCCTTGGTTTCGCCGAAGATCTTGATCGAGCCGTCGTCTTCGATGTCGATCGAAGCCTTGGTCTCTTCGc                                        <  5:251752/136‑1 (MQ=255)                                                                                         gATCTTGCCGATCTCGGCCTCAGCGGTGATGCCCAGGATGCGCTACTTGGCAGCATCTGCCGCTTCCTTGGTTTCGCCGAAGATCTTGATCGAGCCGTCGTCTTCGATGTCGATCGAAGCCTTTGTCTCTTCg                                         >  7:7315/1‑133 (MQ=255)                                                                                                 cGATCTCGGCCTCAGCGGTGATGCCCAGGATGCGCTACTTGGCAGCATCTGCCGCTTCCTTGGTTTCGCCGAAGATCTTGATCGAGCCGTCGTCTTAGAGGTGGATCGAAGCCTTGGTCTCTTCGCAGATGGcgcg                              >  8:238231/1‑136 (MQ=255)                                                                                                  gATCTCGGCCTCAGCGGTGATGCCCAGGATGCGCTACTTGGCAGCATCTGCCGCTTCCTTGGTTTCGCCGAAGATCTTGATCGAGCCGTCGTCTTCGATGTCGATCGAAGCCTTGGTCTCTTCGCAGATGGcgcg                              >  7:165856/1‑135 (MQ=255)                                                                                                        ggCCTCAGCGGTGATGCCCAGGATGCGCTACTTGGCAGCATCTGCCGCTTCCTTGGTTTCGCCGAAGATCTTGATCGAGCCGTCGTCTTCGATGTCGATCGAAGCCTTGGTCTCTTCGCAGATGGCGCGAAtggtg                       <  5:198077/136‑1 (MQ=255)                                                                                                              aGCGGTGATGCCCAGGATGCGCTACTTGGCAGCATCTGCCGCTTCCTTGGTTTCGCCGAAGATCTTGATCGAGCCGTCGTCTTCGATGTCGATCGAAGCCTTGGTCTCTTCGCAGATGGCGCGAATGGTGGcgccg                 <  3:127983/136‑1 (MQ=255)                                                                                                                      tGCCCAGGATGCGCTACTTGGCAGCATCTGCCGCTTCCTTGGTTTCGCCGAAGATCTTGATCGAGCCGTCGTCTTCGATGTCGATCGAAGCCTTGGTCTCTTCGCAGATGGCGCGAATGGTGGCGCCGCCTTTAcc         <  8:22167/136‑1 (MQ=255)                                                                                                                      tGCCCAGGATGCGCTACTTGGCAGCATCTGCCGCTTCCTTGGTTTCGCCGAAGATCTTGATCGAGCCGTCGTCTTCGATGTCGATCGAAGCCTTGGTCTCTTCGCAGATGGCGCGAATGGTGGCGCCGCCTTTAcc         <  3:339854/136‑1 (MQ=255)                                                                                                                             gATGCGCTACTTGGCAGCATCTGCCGCTTCCTTGGTTTCGCCGAAGATCTTGATCGAGCCGTCGTCTTCGATGTGGATCGAAGCCGTGGTGTCTTCGCAGATGGCGCGAATGGTGGCGCCGCCGTTAGCGATGGCg  >  3:28422/1‑136 (MQ=255)                                                                                                                                     |                                                                                                                                GCATGGAGATGTGCACCAAACCGTCCTTGCCAGGCAGGATGTTGACGAAGGCGCCGAAGTCGACAATGCGCTCAACCTTGCCGACGTAGATCTTGCCGATCTCGGCCTCAGCGGTGATGCCCAGGATGCGCTGCTTGGCAGCATCTGCCGCTTCCTTGGTTTCGCCGAAGATCTTGATCGAGCCGTCGTCTTCGATGTCGATCGAAGCCTTGGTCTCTTCGCAGATGGCGCGAATGGTGGCGCCGCCTTTACCGATGACG  >  ACB143/5345473‑5345732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑