Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB143	5,386,500	C→A	intergenic (‑509/+1142)	CJ019_05070 ← / ← nptII	hypothetical protein/neomycin phosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	5,386,500	0	C	A	100.0%	36.4 / NA	12	intergenic (‑509/+1142)	CJ019_05070/nptII	hypothetical protein/neomycin phosphotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (8/4);  total (8/4)

AGCCAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGTCGT‑CA‑GGATC‑‑A‑‑TCCGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGG  >  ACB143/5386370‑5386605                                                                                                                                   |                                                                                                                aGCCAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCcgtggt                                                                                                            >  5:31700/1‑136 (MQ=255)  gCCAGCAGTCTAACTAAAAAGTCATCCCAAATCGCGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagtggtg                                                                                                           <  8:134962/136‑2 (MQ=255)  gCCAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagtggt                                                                                                            <  1:154906/135‑1 (MQ=255)  gCCAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagtggt                                                                                                            <  5:77048/135‑1 (MQ=255)   ccAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagtggtg                                                                                                           >  4:51178/1‑134 (MQ=255)   ccAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagtggtg                                                                                                           >  5:189997/1‑134 (MQ=255)   ccAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagtggtg                                                                                                           >  1:83707/1‑134 (MQ=255)      gcagTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagtggtgtacg                                                                                                       <  1:100259/136‑6 (MQ=255)       cagTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagtggtgtacgg                                                                                                      <  5:158182/136‑7 (MQ=255)                                                                  aTCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggggg                                          <  7:193529/135‑1 (MQ=255)                                                                         tCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTa                                  >  4:348148/1‑136 (MQ=255)                                                                               aTTAAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGGGGTGTACGGTAC‑‑AACCCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcgc                            <  6:189997/136‑1 (MQ=255)                                                                                            atatGACATGTCGGCCGGCTTGTCGACGCCGGCGGTCTCAGTGGTGTACGGTACAAA‑‑CCCCGACTCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg               <  5:350171/136‑1 (MQ=255)                                                                                            atatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg               <  2:323315/136‑1 (MQ=255)                                                                                                     ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCt      >  2:269571/1‑136 (MQ=255)                                                                                                     ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCt      >  2:164309/1‑136 (MQ=255)                                                                                                     ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCt      >  1:257975/1‑136 (MQ=255)                                                                                                     ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCt      >  8:192406/1‑136 (MQ=255)                                                                                                        ggccggcTTGTCGACGACGGCGGTCTCAGTGGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTgg   >  4:338331/1‑136 (MQ=255)                                                                                                         gccggcTTGTCGACGACGGCGGTCTCAGTGGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCCCCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTggg  >  5:93592/1‑136 (MQ=255)                                                                                                         gccggcTTGTCGACGACGGCGGTCTCAGTGGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCGTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTTCCCTTggg  >  3:206195/1‑136 (MQ=255)                                                                                                                                   |                                                                                                                AGCCAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGTCGT‑CA‑GGATC‑‑A‑‑TCCGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGG  >  ACB143/5386370‑5386605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑