Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB143	5,386,514	T→C	intergenic (‑523/+1128)	CJ019_05070 ← / ← nptII	hypothetical protein/neomycin phosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	5,386,514	0	T	C	100.0%	39.2 / NA	12	intergenic (‑523/+1128)	CJ019_05070/nptII	hypothetical protein/neomycin phosphotransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/4);  total (8/4)

ATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGTCGT‑CA‑GGATC‑‑A‑‑TCCGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGG  >  ACB143/5386435‑5386605                                                                                      |                                                                                            aTCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCggggg                                          <  7:193529/135‑1 (MQ=255)        tCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTa                                  >  4:348148/1‑136 (MQ=255)              aTTAAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGGGGTGTACGGTAC‑‑AACCCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcgc                            <  6:189997/136‑1 (MQ=255)                           atatGACATGTCGGCCGGCTTGTCGACGCCGGCGGTCTCAGTGGTGTACGGTACAAA‑‑CCCCGACTCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg               <  5:350171/136‑1 (MQ=255)                           atatGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg               <  2:323315/136‑1 (MQ=255)                                    ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCt      >  1:257975/1‑136 (MQ=255)                                    ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCt      >  2:164309/1‑136 (MQ=255)                                    ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCt      >  2:269571/1‑136 (MQ=255)                                    ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCt      >  8:192406/1‑136 (MQ=255)                                       ggccggcTTGTCGACGACGGCGGTCTCAGTGGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTgg   >  4:338331/1‑136 (MQ=255)                                        gccggcTTGTCGACGACGGCGGTCTCAGTGGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCCCCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTggg  >  5:93592/1‑136 (MQ=255)                                        gccggcTTGTCGACGACGGCGGTCTCAGTGGTGTACGGTACAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCGTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTTCCCTTggg  >  3:206195/1‑136 (MQ=255)                                                                                      |                                                                                            ATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGTCGT‑CA‑GGATC‑‑A‑‑TCCGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTTGGG  >  ACB143/5386435‑5386605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑