Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB143	445,359	A→G	H76R (CAC→CGC)	argD_1 →	Acetylornithine aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	445,359	0	A	G	97.0%	238.4 / ‑2.0	67	H76R (CAC→CGC)	argD_1	Acetylornithine aminotransferase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (38/27);  total (39/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.14e-01

CCTGAGCGCCTGATGCCCGGCACCGAACGTGCAGCGCAGGTATTCGTGCGTGGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCACAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTGTGTCAGAGTACCGGCAGTGACCAGGC  >  ACB143/445226‑445491                                                                                                                                      |                                                                                                                                     ccTGAGCGCCTGATGCCCGGCACCGAACGTGCAGCGCAGGTATTCGTGCGTGGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCcgca                                                                                                                                    >  5:35998/1‑136 (MQ=255) ccTGAGCGCCTGATGCCCGGCACCGAACGTGCAGCGCAGGTATTCGTGCGTGGCCAGGGCTCGAGGTTGTGGGACAACGAAGGGAATGCCTACCTGGACTTCCCTCAGGGCGGTGCGGTCAACAGCTTGGGCcgca                                                                                                                                    >  4:200017/1‑136 (MQ=255)     aGCGCCTGATGCCCGGCACCGAACGTGCAGCGCAGGTTTTCGTGCGTGGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGcc                                                                                                                                 <  7:105051/135‑1 (MQ=255)     aGCGCCTGATGCCCGGCACCGAACGTGCAGCGCAGGTATTCGTGCGTGGCCAGGGCGCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGcc                                                                                                                                 <  1:130282/135‑1 (MQ=255)        gcCTGATGCCCGGCACCGAACGTGCAGCGCAGGTATTCGTGCGTGGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAg                                                                                                                             >  1:51233/1‑136 (MQ=255)         ccTGATGCCCGGCACCGAACGTGCAGCGCAGGTATTCGTGCGTGGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGc                                                                                                                            <  3:332797/136‑1 (MQ=255)         ccTGATGCCCGGCACCGAACGTGCAGCGCAGGTATTCGTGCGTGGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGc                                                                                                                            <  2:70516/136‑1 (MQ=255)         ccTGATGCCCGGCACCGAACGTGCAGCGCAGGTATTCGTGCGTGGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGc                                                                                                                            <  3:101165/136‑1 (MQ=255)             aTGCCCGGCACCGAACGTGCAGCGCAGGTATTCGTGCGTGGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGc                                                                                                                        >  8:48326/1‑136 (MQ=255)               gCCCGGCACCGAACGTGCAGCGCAGGTATTCGTGCGTGGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGcc                                                                                                                                 >  5:368676/1‑125 (MQ=255)               gCCCGGCACCGAACGTGCAGCGCAGGTATTCGTGCGTGGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGcc                                                                                                                                 <  6:368676/125‑1 (MQ=255)                cccGGCACCGAACGTGCAGCGCAGGTATTCGTGCGTGGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTgg                                                                                                                     <  1:244633/136‑1 (MQ=255)                   ggCACCGAACGTGCAGCGCAGGTATTCGTGCGTGGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTCCCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCCGCGTGCTGGTc                                                                                                                   >  4:284293/1‑135 (MQ=255)                       ccGAACGTGCAGCGCAGGTATTCGTGCGTGGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGc                                                                                                              >  8:225931/1‑136 (MQ=255)                              tGCAGCGCAGGTATTCGTGCGTGGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTTCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGc                                                                                                       <  5:311422/136‑1 (MQ=255)                              tGCAGCGCAGGTATTCGTGCGTGGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACATGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTggg                                                                                                        >  6:211792/1‑135 (MQ=255)                                     cAGGTATTCGTGCGTGGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAgg                                                                                                <  6:337765/136‑1 (MQ=255)                                     cAGGTATTCGTGCGTGGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAgg                                                                                                <  4:64536/136‑1 (MQ=255)                                     cAGGTATTCGTGCGTGGCCAGGGCTCGTGGTTGTCGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAgg                                                                                                >  5:56319/1‑136 (MQ=255)                                              gtgcgtgGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCg                                                                                        <  7:1072/135‑1 (MQ=255)                                                gcgtgGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGtt                                                                                      >  2:30228/1‑135 (MQ=255)                                                     gCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCaa                                                                                >  2:304777/1‑136 (MQ=255)                                                          ggCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCcgg                                                                           <  2:20931/136‑1 (MQ=255)                                                             tCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCACCCcgggct                                                                        >  5:57242/1‑136 (MQ=255)                                                                  gTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCcgggctcgggc                                                                   >  6:244311/1‑136 (MQ=255)                                                                  gTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCcgggctcgggc                                                                   >  6:118823/1‑136 (MQ=255)                                                                  gTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCcgggctcgggc                                                                   >  1:304099/1‑136 (MQ=255)                                                                   ttGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCcgggctcgggct                                                                  >  8:120640/1‑136 (MQ=255)                                                                     gtgGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCtt                                                                 <  5:191115/135‑1 (MQ=255)                                                                       gggACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCca                                                              >  3:330496/1‑136 (MQ=255)                                                                        ggACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCca                                                              >  5:168016/1‑135 (MQ=255)                                                                            aaCGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAAcc                                                         >  1:279563/1‑136 (MQ=255)                                                                             aCGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCg                                                        <  4:203524/136‑1 (MQ=255)                                                                                aaGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTgg                                                     >  4:284964/1‑136 (MQ=255)                                                                                aaGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTgg                                                     >  8:116734/1‑136 (MQ=255)                                                                                 aGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGc                                                    >  4:134582/1‑136 (MQ=255)                                                                                 aGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGc                                                    >  8:280316/1‑136 (MQ=255)                                                                                  gggCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCt                                                   <  3:284964/136‑1 (MQ=255)                                                                                  gggCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCt                                                   <  7:197999/136‑1 (MQ=255)                                                                                           tACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGc                                          >  3:171682/1‑136 (MQ=255)                                                                                                ggACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGt                                      >  3:376127/1‑135 (MQ=255)                                                                                                ggACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGAGGCTTGCTGGGGCTGGt                                      >  3:242770/1‑135 (MQ=255)                                                                                                 gACCTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCa                                    <  5:270581/136‑1 (MQ=255)                                                                                                   cTTCACTCCGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCCGGTCAAAGCGTTGGTCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAAc                                  <  3:276463/136‑1 (MQ=255)                                                                                                     tCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCg                                >  8:199115/1‑136 (MQ=255)                                                                                                      cACTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCgg                               >  5:218735/1‑136 (MQ=255)                                                                                                       aCTCAGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCgg                               <  1:231299/135‑1 (MQ=255)                                                                                                         tCACGGCGGTGCGGTCAACAGCTAGGGCCGCAGCCCCAGCGTGATCGTCAAAGCGTTGGGCAACCAGGATCACGCGTTGATCAACCCGGGCTCGGGATTCCACAACCGTGGCTTGCTGGCGCTGGTCAACCGGcag                            >  4:363867/1‑134 (MQ=255)                                                                                                          cAGGGCGGTGCGGTCAACAGCTTGGGCCACAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGtt                                                                                      <  8:240520/77‑1 (MQ=255)                                                                                                          cAGGGCGGTGCGGTCAACAGCTTGGGCCACAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGtt                                                                                      >  7:240520/1‑77 (MQ=255)                                                                                                           aGGGCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCtgtg                          >  6:335894/1‑136 (MQ=255)                                                                                                            gggCGGTGCGGTCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTgtgt                         <  1:304777/136‑1 (MQ=255)                                                                                                                ggtgcggtCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTGTGTCa                       >  6:362865/1‑134 (MQ=255)                                                                                                                    cggtCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTGTGTCAGAGTAc                 >  4:193791/1‑136 (MQ=255)                                                                                                                     ggtCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTGTGTCAGAGTAcc                >  5:261397/1‑136 (MQ=255)                                                                                                                     ggtCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTGTGTCAGAGTAcc                >  8:15554/1‑136 (MQ=255)                                                                                                                     ggtCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTGTGTCAGAGTAcc                >  6:180311/1‑136 (MQ=255)                                                                                                                      gtCAACCGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAACGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTGTGTCAGAGTACCg               <  5:111481/136‑1 (MQ=255)                                                                                                                      gtCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTGTGTCAGAGTACCg               <  5:22406/136‑1 (MQ=255)                                                                                                                      gtCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTGTGTCAGAGTACCg               <  2:28433/136‑1 (MQ=255)                                                                                                                      gtCAACAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTGTGTCAGAGTACCg               <  1:75965/136‑1 (MQ=255)                                                                                                                          aCAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCACCCGGCTGTGTCAGAGTACCGGCAg           >  1:31576/1‑136 (MQ=255)                                                                                                                           catgttGGGCCGCAGCCCCAGCGTGCTGGTCAAAGGGTTGTGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTGTGTCAGAGTACCGGCAGt          <  3:158133/132‑1 (MQ=255)                                                                                                                           cAGCTTGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTGTGTCAGAGTACCGGCAGt          <  2:279563/136‑1 (MQ=255)                                                                                                                               ttGGGCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTGTGTCAGAGTACCGGCAGTGAc       >  4:161389/1‑135 (MQ=255)                                                                                                                                 gggCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTGTGTCAGAGTACCGGCAGTGACCAg    >  6:317854/1‑136 (MQ=255)                                                                                                                                 gggCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTGTGTCAGAGTACCGGCAGTGACCAg    >  6:176001/1‑136 (MQ=255)                                                                                                                                  ggCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTGTGTCAGAGTACCGGCAGTGACCAgg   <  6:168016/136‑1 (MQ=255)                                                                                                                                   gCCGCAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTGTGTCAGAGTACCGGCAGTGACCAGGc  >  4:6445/1‑136 (MQ=255)                                                                                                                                      |                                                                                                                                     CCTGAGCGCCTGATGCCCGGCACCGAACGTGCAGCGCAGGTATTCGTGCGTGGCCAGGGCTCGTGGTTGTGGGACAACGAAGGGCATGCCTACCTGGACTTCACTCAGGGCGGTGCGGTCAACAGCTTGGGCCACAGCCCCAGCGTGCTGGTCAAAGCGTTGGGCAACCAGGCTCAGGCGTTGATCAACCCGGGCTCGGGCTTCCACAACCGTGGCTTGCTGGGGCTGGTCAACCGGCTGTGTCAGAGTACCGGCAGTGACCAGGC  >  ACB143/445226‑445491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑