Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB143 5,620,966 A→G Y285H (TAT→CAT)  CJ019_05282 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,620,9660AG100.0% 191.3 / NA 50Y285H (TAT→CAT) CJ019_05282hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/25);  total (25/25)

ACACCGAGGGCGACCAGCGCAACCAGGCCCGCCAGCAGCCCCAGGCGTTTGTTGCGCCGGTGGCGCTTGAGCAACTGCTGCTTGCGGCTCACGCTTGATACACCGGCACAGGGTTGCACCAGCGGTCCTTATACTCACGGTCAGCACGGCCGAACGAGAACCGCAGCGGCTTGTTGCGTGACCGGGCGTCTTCCCAGGCGGCCTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGA  >  ACB143/5620834‑5621094
                                                                                                                                    |                                                                                                                                
acacCGAGGGCGACCAGCGCAACCAGGCCCGCCAGCAGCCCCAGGCGTTTGTTGCGCCGGTGGCGCTTGAGCAACTGCTGCTTGCGGCTCACGCTTGATACACCGGCACAGTGTTGCACCAGCGGTCCTTATGCTc                                                                                                                               <  3:60688/136‑1 (MQ=255)
acacCGAGGGCGACCAGCGCAACCAGGCCCGCCAGCAGCCCCAGGCGTTTGTTGCGCCGGTGGCGCTTGAGCAACTGCTGCTTGCGGCTCACGCTTGATACACCGGCACAGGGTTGCACCAGCGGTCCTTATGCt                                                                                                                                >  1:1527/1‑135 (MQ=255)
               aGCGCAACCAGGCCCGCCAGCAGCCCCAGGCGTTTGTTGCGCCGGTGGCGCTTGAGCAACTGCTGCTTGCGGCTCACGCTTGATACACCGGCACAGGGTTGCACCCGCGGTCCTTATGCTCACGGTCAGCACGGcc                                                                                                                >  2:203641/1‑136 (MQ=255)
               aGCGCAACCAGGCCCGCCAGCAGCCCCAGGCGTTTGTTGCGCCGGTGGCGCTTGAGCAACTGCTGCTTGCGGCTCACGCTTGATACACCGGCACAGGGTTGCACCAGCGGTCCTTATGCTCACGGTCAGCACGGcc                                                                                                                >  6:346336/1‑136 (MQ=255)
                          gCCCGCCAGCAGCCCCAGGCGTTTGTTGCGCCGGTGGCGCTTGAGCAACTGCTGCTTGCGGCTCACGCTTGATACACCGGCACAGGGTTGCACCAGCGGTCCTTATGCTCACGGTCAGCACGGCCGAACGAGAAcc                                                                                                     <  6:147343/136‑1 (MQ=255)
                          gCCCGCCAGCAGCCCCAGGAGTTTGTTGCTCCGGTGTCTCTTGAGCAACTGCTGCTTGCTGCTCACGCTTGATAGTCCGTCACATGGTTGCACCAGCGGTCCTTATGCTCACGGTCAGCACGGCCGAACTAGaaac                                                                                                     <  2:138749/136‑3 (MQ=255)
                           cccGCCAGCAGCCCCAGGCGTTTGTTGCGCCGGTGGCGCTTGAGCAACTGCTGCTTGCGGCTCACGCTTGATACACCGGCACAGGGTTGCACCAGCGGTCCTTATGCTCACGGTCAGCACGGCCGAACGAGAACCg                                                                                                    <  1:172557/136‑1 (MQ=255)
                             cGCCAGCAGCCCCAGGCGTTTGTTGCGCCGGTGGCGCTTGAGCAACTGCTGCTTGCGGCTCACGCTTGATACACCGGCACAGGGTTGCACCAGCGGTCCTTATGCTCACGGTCAGCACGGCCGAACGAGAACCg                                                                                                    >  2:105651/1‑134 (MQ=255)
                             cGCCAGCAGCCCCAGGCGTTTGTTGCGCCGGTGGCGCTTGAGCAACTGCTGCTTGCGGCTCACGCTTGATACACCGGCACAGGGTTGCACCAGCGGTCCTTATGCTCACGGTCAGCACGGCCGAACGAGAACCGCa                                                                                                  <  6:240193/136‑1 (MQ=255)
                              gCCAGCAGCCCCAGGCGTTTGTTGCGCCGGTGGCGCTTGAGCAACTGCTGCTTGCGGCTCACGCTTGATACACCGGCACAGGGTTGCACCAGCGGTCCTTATGCTCACGGTCAGCACGGCCGAACGAGAACCGCAg                                                                                                 >  5:255192/1‑136 (MQ=255)
                              gCCAGCAGCCCCAGGCGTTTGTTGCGCCGGTGGCGCTTGAGCAACTGCTGCTTGCGGCTCACGCTTGATACACCGGCACAGGGTTGCACAAGCGGTCCTTATGCTCACGGTCAGCACGGCCGAACGAGAAcagcag                                                                                                 >  2:30418/1‑136 (MQ=255)
                                                 tgttgCGCCGGTGGCGCTTGAGCAACTGCTGCTTGCGGCTCACGCTTGATACACCGGCACAGGGTTGCACCAGCGGTCCTTATGCTCACGGTCAGCACGGCCGAACGAGAACCGCAGCGGCTTGTTGCGTGACCg                                                                               <  6:272666/135‑1 (MQ=255)
                                                    tgCGCCGGTGGCGCTTGAGCAACTGCTGCTTGCGGCTCACGCTTGATACACCGGCACAGGGTTGCACCAGCGGTCCTTATGCTCACGGTCAGCACGGCCGAACGAGAACCGCAGCGGCTTGTTGCGTGACCGGGCg                                                                           >  6:188720/1‑136 (MQ=255)
                                                    tgCGCCGGTGGCGCTTGAGCAACTGCTGCTTGCGGCTCACGCTTGATACACCGGCACAGGGTTGCACCAGCGGTCCTTATGCTCACGGTCAGCACGGCCGAACGAGAACCGCAGCGGCTTGTTGCGTGACCGGGCg                                                                           >  5:132281/1‑136 (MQ=255)
                                                    tgCGCCGGTGGCGCTTGAGCAACTGCTGCTTGCGGCTCACGCTTGATACACCGGCACAGGGTTGCACCAGCGGTCCTTATGCTCACGGTCAGCACGGCCGAACGAGAACCGCAGCGGCTTGTTGCGTGACCGGGCg                                                                           >  4:260046/1‑136 (MQ=255)
                                                          ggTGGCGCTTGAGCAACTGCTGCTTGCGGCTCACGCTTGATACACCGGCACAGGGTTGCACCAGCGGTCCTTATGCTCACGGTCAGCACGGCCGAACGAGAACCGCAGCGGCTTGTTGCGTGACCGGGCGTCTTcc                                                                     <  4:21787/136‑1 (MQ=255)
                                                           gTGGCGCTTGAGCAACTGCTGCTTGCGGCTCACGCTTGATACACCGGCACAGGGTTGCACCAGCGGTCCTTATGCTCACGGTCAGCACGGCCGAACGAGAACCGCAGCGGCTTGTTGCGTGACCGGGCGTCTTccc                                                                    <  1:46858/136‑1 (MQ=255)
                                                               cgcTTGAGCAACTGCTGCTTGCGGCTCACGCTTGATACACCGGCACAGGGTTGCACCAGCGGTCCTTATGCTCACGGTCAGCACGGCCGAACGAGAACCGCAGCGGCTTGTTGCGTGACCGGGCGTCTTCCCAggc                                                                <  4:266522/136‑1 (MQ=255)
                                                                 cTTGAGCAACTGCTGCTTGCGGCTCACGCTTGATACACCGGCACAGGGTTGCACCAGCGGTCCTTATGCTCACGGTCAGCACGGCCGAACGAGAACCGCAGCGGCTTGTTGCGTGACCGGGCGTCTTCCCAggcgg                                                              <  8:87243/136‑1 (MQ=255)
                                                                 cTTGAGCAACTGCTGCTTGCGGCTCACGCTTGATACACCGGCACAGGGTTGCACCAGCGGTCCTTATGCTCACGGTCAGCACGGCCGAACGAGAACCGCAGCGGCTTGTTGCGTGACCGGGCGTCTTCCCAggcgg                                                              <  6:14062/136‑1 (MQ=255)
                                                                 cTTGAGCAACTGCTGCTTGCGGCTCACGCTTGATACACCGGCACAGGGTTGCACCAGCGGTCCTTATGCTCACGGTCAGCACGGCCGAACGAGAACCGCAGCGGCTTGTTGCGTGACCGGGCGTCTTCCCAggcgg                                                              <  3:198064/136‑1 (MQ=255)
                                                                    gAGCAACTGCTGCGTGCGGCTCACGCTTGATACACCGGCACAGGGTTGCACCAGCGGTCCTTATGCTCACGGTCAGCACGGCCGAACGAGAACCGCAGCGGCTTGTTGCGTGACCGGGCGTCTTCCCAGGCGGccg                                                           >  5:98712/1‑135 (MQ=255)
                                                                             ctgctTGCGGCTCACGCTTGATACACCGGCACAGGGTTGCACCAGCGGTCCTTATGCTCACGGTCAGCACGGCCGAACGAGAACCGCAGCGGCTTGTTGCGTGACCGGGCGTCTTCCCAGGCGGCCTGGGTATTg                                                   <  4:325111/135‑1 (MQ=255)
                                                                             ctgctTGCGGCTCACGCTTGATACACCGGCACAGGGTTGCACCAGCGGTCCTTATGCTCACGGTCAGCACGGCCGAACGAGAACCGCAGCGGCTTGTTGCGTGACCGGGCGTCTTCCCAGGCGGCCTGGGTATTg                                                   <  7:207675/135‑1 (MQ=255)
                                                                                  tGCGGCTCACGCTTGATACACCGGCACAGGGTTGCAACAGCGGTCCTTATGCTCACGGTCAGCACGGCCGAACGAGAAACGCAGCGGCTTGTTGCGTGACCGCGCGTCTTCCCAGGACGCCAGGGTATTTAGGAAg                                             >  8:243427/1‑136 (MQ=255)
                                                                                    cGGCTCACGCTTGATACACCGGCACAGGGTTGCACCAGCGGTCCTTATGCTCACGGTCAGCACGGCCGAACGAGAACCGCAGCGGCTTGTTGCGTGACCGGGCGTCTTCCCAGGCGGCCTGGGTATTGAGGAAGc                                            <  6:315103/135‑1 (MQ=255)
                                                                                        tCACTCTTGATACACATGCAGAGGGTTTCACCAGCGGTCCTTCTGCTCACGGTCAGCACGGCCGAACGAGAACCGCAGCGGCTTGTTGCGTGACCGTGCGTCTTCCCAGGCGGCCTGGGTATTGAGGAAGCTCAGc                                       <  8:313646/136‑1 (MQ=255)
                                                                                          aCGCTTGATACACCGGCACAGGGTTGCACCAGCGGTCCTTATGCTCACGGTCAGCACGGCCGAACGAGAACCGCAGCGGCTTGTTGCGTGACCGGGAGTCTTCCCAGGCTGCCTGGGTATTGAGGAAGCTCAGc                                       <  5:198063/134‑1 (MQ=255)
                                                                                            gCTTGATACACCGGCACAGGGTTGCACCAGAGTTCCTTAAGCTCACGGTCAGCACGGCCGAACGAGAACCGCAGCGGCTTGTTGCGTGACCGGGCGTCTTCCCAGGCGGCCTGGGTATTGAGGAAGCTCAGCACGc                                   <  5:95495/136‑1 (MQ=255)
                                                                                             cTTGATACACCGGCACAGGGTTGCACCAGCGGTCCTTATGCTCACGGTCAGCACGGCCGAACGAGAACCGCAGCGGCTTGTTGCGTGACCGGGCGTCTTCCCAGGCGGCCTGGGTATTGAGGAAGCTCAGCACGCt                                  <  8:62840/136‑1 (MQ=255)
                                                                                              ttGATACACCGGCACAGGGTTGCACCAGCGGTCCTTATGCTCACGGTCAGCACGGCCGAACGAGAACCGCAGCGGCTTGTTGCGTGACCGGGCGTCTTCCCAGGCGGCCTGGGTATTGAGGAAGCTCAGCACGCTg                                 <  4:198705/136‑1 (MQ=255)
                                                                                                gATACACCGGCACAGGGTTGCACCAGCGGTCCTTATGCTCACGGTCAGCACGGCCGAACGAGAACCGCAGCGGCTTGTTGCGTGACCGGGCGTCTTCCCAGGCGGCCTGGGTATTGAGGAAGCTCAGCACGCTg                                 <  3:290745/134‑1 (MQ=255)
                                                                                                         gCACAGGGTTGCACCAGCGGTCCTTATGCTCACGGTCAGCACGGCCGAACGAGAACCGCAGCGGCTTGTTGCGTGACCGGGCGTCTTCCCAGGCGGCCTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTg                        >  6:136856/1‑134 (MQ=255)
                                                                                                           acaGGGTTGCACCAGCGGTCCTTATGCTCACGGTCAGCACGGCCGAATGAGAACCGCAGCGGCTTGTTGCGTGACCGGGCGTCTTCCCAGGCGGCCTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAgg                    <  2:344501/136‑1 (MQ=255)
                                                                                                           acaGGGTTGCACCAGCGGTCCTTATGCTCACGGTCAGCACGGCCGAACGAGAACCGCAGCGGCTTGTTGCGTGACCGGGCGTCTTCCCAGGCGGCCTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAgg                    >  7:43815/1‑136 (MQ=255)
                                                                                                            caGGGTTGCACCAGCGGTCCTTATGCTCACGGTCAGCACGGCCGAACGAGAACCGCAGCGGCTTGTTGCGTGACCGGGCGTCTTCCCAGGCGGCCTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGc                   <  2:169831/136‑1 (MQ=255)
                                                                                                                 ttGCACCAGCGGTCCTTATGCTCACGGTCAGCACGGCCGAACGAGAACCGCAGCGGCTTGTTGCGTGACCGGGCGTCTTCCCAGGCGGCCTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTgg              <  2:202465/136‑1 (MQ=255)
                                                                                                                  tGCACCAGCGGTCCTTATGCTCACGGTCGGCACGGCCGAACGAGAACCGCAGCGGCTTGTTGCGTGACCGGGCGTCTTCCCAGGCGGCCTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGt             >  1:203076/1‑136 (MQ=255)
                                                                                                                     aCCAGCGGTCCTTATGCTCACGGTCAGCACGGCCGAACGAGAACCGCAGCGGCTTGTTGCGTGACCGGGCGTCTTCCCAGGCGGCCTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGttt           >  5:90296/1‑135 (MQ=255)
                                                                                                                       cAGCGGTCCTTATGCTCACGGTCAGCACGGCCGAACGAGAACCGCAGCGGCTTGTTGCGTGACCGGGCGTCTTCCCAGGCGGCCTGGGTATTTAGGAAGCTCAGCACGCTGCCGGGGCGGAAGGAGTTGGTTTAAg        >  2:187668/1‑136 (MQ=255)
                                                                                                                       cAGCGGTCCTTATGCTCACGGTCAGCACGGCCGAACGAGAACCGCAGCGGCTTGTTGCGTGACCGGGCGTCTTCCCAGGCGGCCTGGGTATTGAGGAAGCTCAGCACGCTGGCTGGGCTGAAGGCCTTGGTTTccg        >  8:34260/1‑134 (MQ=255)
                                                                                                                            gTCCTTATGCTCACGGTCAGCACGGCCGAACGAGAACCGCAGCGGCTTGTTGCGTGACCGGGCGTCTTCCCCCGCGGCCTGGGGATTGAGGAAGCACAGCACGCGGGCAGGGGTTCACGCCTTGGTGTCAggg      >  7:122489/1‑133 (MQ=255)
                                                                                                                            gTCCTTATGCTCACGGTCAGCACGGCCGAACGAGAACCGCAGCGGCTTGTTGCGTGACCGGGCGTCTTCCCAGGCGGCCTGGGTATTGAGGAAGCTCCGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTc    >  6:96452/1‑135 (MQ=255)
                                                                                                                            gTCCTTATGCTCACGGTCAGCACGGCCGAACGAGAACCGCAGCGGCTTGTTGCGTGACCGGGCGTCTTCCCAGGCGGCCTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTc    >  1:12090/1‑135 (MQ=255)
                                                                                                                            gTCCTTATGCTCACGGTCAGCACGGCCGAACGAGAACCGCAGCGGCTTGTTGCGTGACCGGGCGTCTTCCCAGGCGGCCTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTc    >  3:312350/1‑135 (MQ=255)
                                                                                                                            gTCCTTATGCTCACGGTCAGCACGGCCGAACGAGAACCGCAGCGGCTTGTTGCGTGACCGGGCGTCTTCCCAGGCGGCCTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTc    >  4:151892/1‑135 (MQ=255)
                                                                                                                            gTCCTTATGCTCACGGTCAGCACGGCCGAACGAGAACCGCAGCGGCTTGTTGCGTGACCGGGCGTCTTCCCAGGCGGCCTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCg   >  4:341141/1‑136 (MQ=255)
                                                                                                                            gTCCTTATGCTCACGGTCAGCACGGCCGAACGAGAACCGCAGCGGCTTGTTGCGTGACCGGGCGTCTTCCCAGGCGGCCTGGGGATTGAGGAAGCTCCGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCg   >  3:330640/1‑136 (MQ=255)
                                                                                                                            gTCCTTATGCTCACGGTCAGCACGGCCGAACGAGAACCGCAGCGGCTTGTTGCGTCACCGGGCGTCTTACCAGGCGGCCGGGGTTTTGATGAAGCTCAGCACCCTGCCTGGGCGGAAGGGCATGGTTTCAGGGTc    >  5:205291/1‑135 (MQ=255)
                                                                                                                             tCCTTATGCTCACGGTCAGCACGGCCGAACGAGAACCGCAGCGGCTTGTTGCGTGACCGGGCGTCTTCCCAGGCGGCCTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGa  <  1:125964/136‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                
ACACCGAGGGCGACCAGCGCAACCAGGCCCGCCAGCAGCCCCAGGCGTTTGTTGCGCCGGTGGCGCTTGAGCAACTGCTGCTTGCGGCTCACGCTTGATACACCGGCACAGGGTTGCACCAGCGGTCCTTATACTCACGGTCAGCACGGCCGAACGAGAACCGCAGCGGCTTGTTGCGTGACCGGGCGTCTTCCCAGGCGGCCTGGGTATTGAGGAAGCTCAGCACGCTGCCTGGGCTGAAGGCCTTGGTTTCAGGGTCGA  >  ACB143/5620834‑5621094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑