Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB143 5,983,547 A→G Y207C (TAC→TGC)  cdhR_8 → HTH‑type transcriptional regulator CdhR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,983,5470AG100.0% 159.4 / NA 42Y207C (TAC→TGC) cdhR_8HTH‑type transcriptional regulator CdhR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/6);  total (36/6)

GGGCGGTATCACTGCAGGGATCGACCTGGCACTGGCGGTGGTCGCCGAGGACCATGGCAAGGCGCTGGCCCTGGAAGTAGCCAAGGTACTGCTGGTAGCCCGCCACCGTCAGGGTGGGCAGACACCCTACGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGATTGCCCGGGTGCAGGCCTATATCGTCGACCACATCGAGCAGGCGTTCACCGTGCAGAAAATGGCCGAACTGGTGGCCATGAGCGGGCGTAA  >  ACB143/5983419‑5983679
                                                                                                                                |                                                                                                                                    
gggCGGTATCACTGCAGGGATCGACCTGGCACTGGCGGTGGTCGCCGAGGACCATGGCAAGGCGCTGGCCCTGGAAGTAGCCAAGGTACTGCTGGTAGCCCGCCACCGTCAGGGTGGGCAGACACCCTGCGGGCCa                                                                                                                               <  4:98349/136‑1 (MQ=255)
                                     gtggtCGCCGAGGACCATGGCAAGGCGCTGGCCCTGGAAGTAGCCAAGGTACTGCTGGTAGCCCGCCACCGTCAGGGTGGGCAGACACCCTGCGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGATTg                                                                                          >  4:12199/1‑136 (MQ=255)
                                          cGCCGAGGACCATGGCAAGGCGCTGGCCCTGGAAGTAGCCAAGGTACTGCTGGTAGCCCGCCACCGTCAGGGTGGGCAGACACCCTGCGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGATTGCCCgg                                                                                     <  7:334812/136‑1 (MQ=255)
                                                         cAAGGCGCTGGCCCTGGAAGTAGCCAAGGTACTGCTGGTAGCCCGCCACCGTCAGGGTGGGCAGACACCCTGCGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGATTGCCCGGGTGCAGGCCTATAtc                                                                      >  3:221651/1‑136 (MQ=255)
                                                         cAAGGCGCTGGCCCTGGAAGTAGCCAAGGTACTGCTGGTAGCCCGCCACCGTCAGGGTGGGCAGACACCCTGCGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGATTGCCCGGGTGCAGGCCTATAtc                                                                      >  4:207658/1‑136 (MQ=255)
                                                          aaGGCGCTGGCCCTGGAAGTAGCCAAGGTACTGCTGGTAGCCCGCCACCGTCAGGGTGGGCAGACACCCTGCGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGATTGCCCGGGTGCAGGCCTATAtcg                                                                     >  4:49965/1‑136 (MQ=255)
                                                          aaGGCGCTGGCCCTGGAAGTAGCCAAGGTACTGCTGGTAGCCCGCCACCGTCAGGGTGGGCAGACACCCTGCGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGATTGCCCGGGTGCAGGCCTATAtcg                                                                     >  7:321108/1‑136 (MQ=255)
                                                               gcTGGCCCTGGAAGTAGCCAAGGTACTGCTGGTAGCCCGCCACCGTCAGGGTGGGCAGACACCCTGCGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGATTGCCCGGGTGCAGGCCTATATCGTCGAc                                                                >  3:212695/1‑136 (MQ=255)
                                                               gcTGGCCCTGGAAGTAGCCAAGGTACTGCTGGTAGCCCGCCACCGTCAGGGTGAGCAGACACCATGCGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGATTGCCCGGGTGCAGGCCTATATCGTCGAc                                                                >  5:136245/1‑136 (MQ=255)
                                                                 tGGCCCTGGAAGTAGCCAAGGTACTGCTGGTAGCCCGCCACCGTCAGGGTGGGCAGACACCCTGCGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGATTGCCCGGGTGCAGGCCTATATAGTCGACca                                                              >  2:310831/1‑136 (MQ=255)
                                                                     ccTGGAAGTAGCCAAGGTACTGCTGGTAGCCCGCCACCGTCAGGGTGGGCAGACACCCTGCGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGATTGCCCGGGTGCAGGCCTATATCGTCGACCACATc                                                          <  8:133155/136‑1 (MQ=255)
                                                                     ccTGGAAGTAGCCAAGGTACTGCTGGTAGCCCGCCACCGTCAGGGTGGGCAGACACCCTGCGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGATTGCCCGGGTGCAGGCCTATATCGTCGACCACATc                                                          >  2:188397/1‑136 (MQ=255)
                                                                           aGTAGCCAAGGTACTGCTGGTAGCCCGCCACCGTCAGGGTGGGCAGACACCCTGCGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGATTGCCCGGGTGCAGGCCTATATCGTCGACCACATCGAGCAg                                                    >  5:286335/1‑136 (MQ=255)
                                                                           aGTAGCCAAGGTACTGCTGGTAGCCCGCCACCGTCAGGGTGGGCAGACACCCTGCGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGATTGCCCGGGTGCAGGCCTATATCGTCGACCACATCGAGCAg                                                    >  3:11876/1‑136 (MQ=255)
                                                                                 cAAGGTACTGCTGGTAGCCCGCCACCGTCAGGGTGGGCAGACACCCTGCGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGAtt                                                                                           <  1:144264/91‑1 (MQ=255)
                                                                                 cAAGGTACTGCTGGTAGCCCGCCACCGTCAGGGTGGGCAGACACCCTGCGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGAtt                                                                                           >  2:144264/1‑91 (MQ=255)
                                                                                  aaGGTACTGCTGGTAGCCCGCCACCGTCAGGGTGGGCAGACACCCTGCGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGATTGCCCGGGTGCAGGCCTATATCGTCGACCACATCGAGCAGGCGTTCa                                             >  4:271818/1‑136 (MQ=255)
                                                                                  aaGGTACTGCTGGTAGCCCGCCACCGTCAGGGTGGGCAGACACCCTGCGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGATTGCCCGGGTGCAGGCCTATATCGTCGACCACATCGAGCAGGCGTTCa                                             >  5:151159/1‑136 (MQ=255)
                                                                                  aaGGTACTGCTGGTAGCCCGCCACCGTCAGGGTGGGCAGACACCCTGCGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGATTGCCCGGGTGCAGGCCTATATCGACGACCACATCGAGCAGGCGTTCa                                             >  6:171037/1‑136 (MQ=255)
                                                                                   aGGTACTGCTGGTAGCCCGCCACCGTCAGGGTGGGCAGACACCCTGCGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGATTGCCCGGGTGCAGGCCTATATCGTCGACCACATCGAGCAGGCGTTCAc                                            >  7:326858/1‑136 (MQ=255)
                                                                                   aGGTACTGCTGGTAGCCCGCCACCGTCAGGGTGGGCAGACACCCTGCGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGATTGCCCGGGTGCAGGCCTATATCGTCGACCACATCGAGCAGGCGTTCAc                                            >  3:233229/1‑136 (MQ=255)
                                                                                       aCTGCTGGTAGCCCGCCACCGTCAGGGTGGGCAGACACCCTGCGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGATTGCCCgg                                                                                     >  7:83685/1‑91 (MQ=255)
                                                                                       aCTGCTGGTAGCCCGCCACCGTCAGGGTGGGCAGACACCCTGCGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGATTGCCCgg                                                                                     <  8:83685/91‑1 (MQ=255)
                                                                                            tgGTAGCCCGCCACCGTCAGGGTGGGCAGACACCCTGCGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGATTGCCCGGGTGCAGGCCTATATCGTCGACCACATCGAGCAGGCGTTCACCGTGCAGa                                    >  5:307672/1‑135 (MQ=255)
                                                                                            tgGTAGCCCGCCACCGTCAGGGTGGGCAGACACCCTGCGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGATTGCCCGGGTGCAGGCCTATATCGTCGACCACATCGAGCAGGCGTTCACCGTGCAGa                                    >  1:346347/1‑135 (MQ=255)
                                                                                            tgGTAGCCCGCCACCGTCAGGGTGGGCAGACACCCTGCGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGATTGCCCGGGTGCAGGCCTATATCGTCGACCACATCGAGCAGGCGTTCACCGTGCAGa                                    >  2:105096/1‑135 (MQ=255)
                                                                                            tgGTAGCCCGCCACCGTCAGGGTGGGCAGACACCCTGCGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGATTGCCCGGGTGCAGGCCTATATCGTCGACCACATCGACCAGGCGTTCACCGTGCAGa                                    >  5:134485/1‑135 (MQ=255)
                                                                                              gTAGCCCGCCACCGTCAGGGTGGGCAGACACCCTGCGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGATTGCCCGGGTGCAGGCCTATATCGTCGACCACATCGAGCAGGCGTTCACCGTGCAGaaaa                                 >  4:57851/1‑136 (MQ=255)
                                                                                                 gCCCGCCACCGTCAGGGTGGGCAGACACCCTGCGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGATTGCCCGGGTGCAGGCCTATATCGTCGACCACATCGAGCAGGCGTTCACCGTGCAGAAAAt                                <  4:200533/134‑1 (MQ=255)
                                                                                                 gCCCGCCACCGTCAGGGTGGGCAGACACCCTGCGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGATTGCCCGGGTGCAGGCCTATATCGTCGACCACATCGAGCAGGCGTTCACCGTGCAGAAAAt                                >  3:200533/1‑134 (MQ=255)
                                                                                                 gCCCGCCACCGTCAGGGTGGGCAGACACCCTGCGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGATTGCCCGGGTGCAGGCCTATATCGTCGACCACATCGAGCAGGCGTTCACCGTGCAGAAAATgg                              >  4:109600/1‑136 (MQ=255)
                                                                                                   ccGCCACCGTCAGGGTGGGCAGACACCCTGCGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGATTGCCCGGGTGCAGGCCTATATCGTCGACCACATCGAGCAGGCGTTCACCGTGCAGAAAATGGcc                            >  5:240257/1‑136 (MQ=255)
                                                                                                   ccGCCACCGTCAGGGTGGGCAGACACCCTGCGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGATTGCCCGGGTGCAGGCCTATATCGTCGACCACATCGAGCAGGCGTTCACCGTGCAGAAAATGGcc                            >  5:248378/1‑136 (MQ=255)
                                                                                                   ccGCCACCGTCAGGGTGGGCAGACACCCTGCGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGATTGCCCGGGTGCAGGCCTATATCGTCGACCACATCGAGCAGGCGTTCACCGTGCAGAAAATGGcc                            >  4:352393/1‑136 (MQ=255)
                                                                                                   ccGCCACCGTCAGGGTGGGCAGACACCCTGCGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGATTGCCCGGGTGCAGGCCTATATCGTCGACCACATCGAGCAGGCGTTCACCGTGCAGAAAATGGcc                            >  4:175037/1‑136 (MQ=255)
                                                                                                   ccGCCACCGTCAGGGTGGGCAGACACCCTGCGGGCCATTGCTGGCTGAGGTACCACGCGATGACTCGCCGATTGCCCGGGTGCAGGCCTATATCGTCGACCACATCGAGCAGGCGTTCACCGTGCAGAAAATGGcc                            >  6:5691/1‑136 (MQ=255)
                                                                                                                       aGACACCCTGCGGGCCATTGCTGGCTGCGGTACCCCGCGATGACTCGCCGATTGCCCGGGTGCAGGCCTATATCGTCGACCACATCGAGCAGGCGTTCACCGTGCAGAAAATGGCCGAACTGGTGGCCATGAGCgg        >  6:84583/1‑136 (MQ=255)
                                                                                                                       aGACACCCTGCGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGATTGCCCGGGTGCAGGCCTATATCGTCGACCACATCGAGCAGGCGTTCACCGTGCAGAAAATGGCCGAACTGGTGGCCATGAGCgg        >  6:306231/1‑136 (MQ=255)
                                                                                                                       aGACACCCTGCGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGATTGCCCGGGTGCAGGCCTATATCGTCGACCACATCGAGCAGGCGTTCACCGTGCAGAAAATGGCCGAACTGGTGGCCATGAGCgg        >  2:113315/1‑136 (MQ=255)
                                                                                                                       aGACACCCTGCGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGATTGCCCGGGTGCAGGCCTATATCGTCGACCACATCGAGCAGGCGTTCACCGTGCAGAAAATGGCCGAACTGGTGGCCATGAGCgg        >  2:4169/1‑136 (MQ=255)
                                                                                                                         acacCCTGCGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGATTGCCCGGGTGCAGGCCTATATCGTCGACCACATCGAGCAGGCGTTCACCGTGCAGAAAATGGCCGAACTGGTGGCCATGAGCGGGc      >  5:206556/1‑136 (MQ=255)
                                                                                                                             ccTGCGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGATTGCCCGGGTGCAGGCCTATATCGTCGACCACATCGAGCAGGCGTTCACCGTGCAGAAAATGGCCGAACTGGTGGCCATGAGCGGGCGTaa  >  6:280795/1‑136 (MQ=255)
                                                                                                                                |                                                                                                                                    
GGGCGGTATCACTGCAGGGATCGACCTGGCACTGGCGGTGGTCGCCGAGGACCATGGCAAGGCGCTGGCCCTGGAAGTAGCCAAGGTACTGCTGGTAGCCCGCCACCGTCAGGGTGGGCAGACACCCTACGGGCCATTGCTGGCTGCGGTACCACGCGATGACTCGCCGATTGCCCGGGTGCAGGCCTATATCGTCGACCACATCGAGCAGGCGTTCACCGTGCAGAAAATGGCCGAACTGGTGGCCATGAGCGGGCGTAA  >  ACB143/5983419‑5983679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑