Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB143 465,344 C→T G214D (GGC→GAC)  rpoD ← RNA polymerase sigma factor RpoD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB143465,3440CT97.7% 138.6 / ‑4.3 43G214D (GGC→GAC) rpoDRNA polymerase sigma factor RpoD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (26/16);  minor base G (1/0);  total (27/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

CAGGTCAGCCAGGGCCTGCAGGGCCTCGATGCTTTCCTTGTGGTTGCGACCATTTTTCTTCAGGACCTTGGAGGTTGCCTGAAGCTGATCGGATACCGCACCGAAGCGTTGGGCTGCGACGACCGGATCCGGGCCGCTCTCGGCCTCTTCCTCGTCGTCACCGCTTTCGGATTCTTCTTCGTCGTCGTCGGACTCTTCCTTCGCCGCAGCGGCCTTGGCACCTGGGATCGGCACTTCTTCGGTTGGCGCGGCAATGTTGTCGTCAG  >  ACB143/465211‑465476
                                                                                                                                     |                                                                                                                                    
cAGGTCAGCCAGGGCCTGCAGGGCCTCGATGCTTTCCTTGTGGTTGCGACCATTTTTCTTCAGGACCTTGGAGGTTGCCTGAAGCTGATCGGATACCGCACCGAAGCGTTGGGCTGCGACGACCGGATCCGGGTCg                                                                                                                                    <  1:344738/136‑1 (MQ=255)
      aGCCAGGGCCTGCAGGGCCTCGATGCTTTCCTTGTGGTTGCGACCATTTTTCTTCAGGACCTTGGAGGTTGCCTGAAGCTGATCGGATACCGCACCGAAGCGTTGGGCTGCGACGACCGGATCCGGGTCGCtctc                                                                                                                               <  7:29278/135‑1 (MQ=255)
      aGCCAGGGCCTGCAGGGCCTCGATGCTTTCCTTGTGGTTGCGACCATTTTTCTTCAGGACCTTGGAGGTTGCCTGAAGCTGATCGGATACCGCACCGAAGCGTTGGGCTGCGACGACCGGATCCGGGTCGCTCTCg                                                                                                                              >  7:70706/1‑136 (MQ=255)
      aGCCAGGGCCTGCAGGGCCTCGATGCTTTCCTTGTGGTTGCGACCATTTTTCTTCAGGACCTTGGAGGTTGCCTGAAGCTGATCGGATACCGAACCGAAGCGTTGGGCTGCGACGACCGGATCCGGGTCGctgacg                                                                                                                              >  3:225426/1‑132 (MQ=255)
      aGCCAGGGCCTGCAGGGCCTCGATGCTTTCCTTGTGGTTGCGACCATTTTTCTCCAGGACCTTGGAGGTTGCCTGAAGCTGATCGGATACCGCACCGAAGCGTTGGGCTGCGACGACAGGATCCGGGTCGCTCTCg                                                                                                                              >  5:368183/1‑136 (MQ=255)
           gggCCTGCAGGGCCTCGATGCTTTCCTTGTGGTTGCGACCATTTTTCTTCAGGACCTTGGAGGTTGCCTGAAGCTGATCGGATACCGCACCGAAGCGTTGGGCTGCGACGACCGGATCCGGGTCGCTCTCGGccgc                                                                                                                         >  1:135898/1‑134 (MQ=255)
           gggCCTGCAGGGCCTCGATGCTTTCCTTGTGGTTGCGACCATTTTTCTTCAGGACCTTGGAGGTTGCCTGAAGCTGATCGGATACCGCACCGAAGCGTTGGGCTGCGACGACCGGATCCGGGTCGCTCTCGGCctc                                                                                                                         >  8:217819/1‑136 (MQ=255)
             gCCTGCAGGGCCTCGATGCTTTCCTTGTGGTTGCGACCATTTTTCTTCAGGACCTTGGAGGTTGCCTGAAGCTGATCGGATACCGCACCGAAGCGTTGGGCTGCGACGACCGGATCCGGGTCGCTCTCGGCCTCtt                                                                                                                       <  4:196627/136‑1 (MQ=255)
                tGCAGGGCCTCGATGCTTTCCTTGTGGTTGCGACCATTTTTCTTCAGGACCTTGGAGGTTGCCTGAAGCTGATCGGATACCGCACCGAAGCGTTGGGCTGCGACGACCGGATCCGGGTCGCTCTCGGGCTCTTCCt                                                                                                                    >  1:43707/1‑136 (MQ=255)
                tGCAGGGCCTCGATGCTTTCCTTGTGGTTGCGACCATTTTTCTTCAGGACCTTGGAGGTTGCCTGAAGCTGATCGGATACCGCACCGAAGCGTTGGGCTGCGACGACCGGATCCGGGTCGCTCTCGGCCTCTTCCt                                                                                                                    >  7:99719/1‑136 (MQ=255)
                    gggCCTCGATGCTTTCCTTGTGGTTGCGACCATTTTTCTTCAGGTCCTTGGAGGTTGCGTGAAGCTGTTCGGATTCCGCACCGAAGCGTTGGGCTGCGACGCCCGGATCCGGGGCGCTCTCGGTCTGTTCgtcgtc                                                                                                                >  2:370891/1‑136 (MQ=255)
                    gggCCTCGATGCTTTCCTTGTGGTTGCGACCATTTTTCTTCAGGACCTTGGAGGTTGCCTGAAGCTGATCGGATACCGCACCGAAGCGTTGGGCTGCGACGACCGGATCCGGGTCGCTCTCGGCCTCTTCCtcgtc                                                                                                                >  7:9429/1‑136 (MQ=255)
                     ggCCTCGATGCTTTCCTTGTGGTTGCGACCATTTTTCTTCAGGACCTTGGAGGTTGCCTGAAGCTGATCGGATACCGCACCGAAGCGTTGGGCTGCGACGACCGGATCCGGGTCGCTCTCGGCCTCTTCCtcgtcg                                                                                                               >  8:70827/1‑136 (MQ=255)
                      gCCTCGATGCTTTCCTTGTGGTTGCGACCATTTTTCTTCAGGACCTTGGAGGTTGCCTGAAGCTGATCGGATACCGCACCGAAGCGTTGGGCTGCGACGACCGGATCCGGGTCGCTCTCGGCCTCTTCCTcgtcgt                                                                                                              <  2:278269/136‑1 (MQ=255)
                          cGATGCTTTCCTTGTGGTTGCGACCATTTTTCTTCAGGACCTTGGAGGTTGCCTGAAGCTGATCGGATACCGCACCGAAGCGTTGGGCTGCGACGACCGGATCCGGGTCGCTCTCGGCCTCTTCCTCGTCGTCAcc                                                                                                          >  1:52748/1‑136 (MQ=255)
                          cGATGCTTTCCTTGTGGTTGCGACCATTTTTCTTCAGGACCTTGGAGGTTGCCTGAAGCTGATCGGATACCGCACCGAAGCGTTGGGCTGCGACGACCGGATCCGGGTCGCTCTCGGCCTCGTCCTcgtcgtaccc                                                                                                          >  5:231477/1‑132 (MQ=255)
                           gATGCTTTCCTTGTGGTTGCGACCATTTTTCTTCAGGACCTTGGAGGTTGCCTGAAGCTGATCGGATACCGCACCGAAGCGTTGGGCTGCGACGACCGGATCCGGGTCGCTCTCGGCCTCTTCCTCGTCGTCACCg                                                                                                         >  5:247028/1‑136 (MQ=255)
                             tGCTTTCCTTGTGGTTGCGACCATTTTTCTTCAGGACCTTGGAGGTTGCCTGAAGCTGATCGGATACCGCACCGAAGCGTTGGGCTGCGACGACCGGATCCGGGTCGCTCTCGGCCTCTTCCTCGTCGTCACCGCt                                                                                                       >  2:283358/1‑136 (MQ=255)
                                    cTTGTGGTTGCGCCCATTTTTCTTCAGGACCTTGGAGGTTGCCTGAAGCTGATCGGATACCGCACCGAAGCGTTGGGCTGCGACGACCGGCTCCGGCTCGCTCTCGGCCTCTTCCTCGTCGTCACCGAGTTCGGAt                                                                                                >  6:102052/1‑136 (MQ=255)
                                    cTTGTGGTTGCGACCATTTTTCTTCAGGACCTTGGAGGTTGCCTGAAGCTGATCGGATACCGCACCGAAGCGTTGGGCTGCGACGACCGGATCCGGGTCGCTCTCGGCCTCTTCCTCGTCGTCACCGCTTTCGGAt                                                                                                >  2:61105/1‑136 (MQ=255)
                                            tGCGACCATTTTTCTTCAGGACCTTGGAGGTTGCCTGAAGCTGATCGGATACCGCACCGAAGCGTTGGGCTGCGACGACCGGATCCGGGTCGCTCTCGGCCTCTTCCTCGTCGTCACCGCTTTCGGAttcttcttc                                                                                        >  5:155977/1‑136 (MQ=255)
                                             gCGACCATTTTTCTTCAGGACCTTGGAGGTTGCCTGAAGCTGATCGGATACCGCACCGAAGCGTTGGGCTGCGACGACCGGATCCGGGTCGCTCTCGGCCTCTTCCTCGTCGTCACCGCTTTCGGATTCTTCTtcg                                                                                       >  6:202837/1‑136 (MQ=255)
                                              cGACCATTTTTCTTCAGGACCTTGGAGGTTGCCTGAAGCTGATCGGATCCCGCACCGAAGCGTTGGGCTGCGACGACCGGATCCGGGTCGCTCTCGGCCTCTTCCTCGTCGTCACCGCTTTCGGATTCTTCTtcg                                                                                       <  7:165487/135‑1 (MQ=255)
                                              cGACCATTTTTCTTCAGGACCTTGGAGGTTGCCTGAAGCTGATCGGATACCGCACCGAAGCGTTGGGCTGCGACGACCGGATCCGGGTCGCTCTCGGCCTCTTCCTCGTCGTCACCGCTTTCGGATTCTTCTtcgt                                                                                      <  2:101049/136‑1 (MQ=255)
                                                   aTTTTTCTTCAGGACCTTGGAGGTTGCCTGAAGCTGATCGGATACCGCACCGAAGCGTTGGGCTGCGACGACCGGATCCGGGTCGCTCTCGGCCTCTTCCTCGTCGTCACCGCTTTCGGATTCTTCTtcgtcgtcg                                                                                 <  6:139296/136‑1 (MQ=255)
                                                                      gAGGTTGCCTGAAGCTGATCGGATACCGCACCGAAGCGTTGGGCTGCGACGACCGGATCCGGGTCGCTCTCGGCCTCTTCCTCGTCGTCACCGCTTTCGGATTCTTCTTCGTCGTCGTCGGACTCTTCCTTcgccg                                                              <  6:182958/136‑1 (MQ=255)
                                                                       aGGTTGCCTGAAGCTGATCGGATACCGCACCGAAGCGTTGGGCTGCGACGACCGGATCCGGGTCGCTCTCGGCCTCTTCCTCGTCGTCACCGCTTTAGGATTCTTCTTCGTCGTCGTAGGGCTCGTACTTagccgc                                                             >  7:283405/1‑136 (MQ=255)
                                                                        ggTTGCCTGAAGCTGATCGGATACCGCACCGAAGCGTTGGGCTGCGACGACCGGATCCGGGTCGCTCTCGGCCTCTTCCTCGTCGTCACCGCTTTCGGATTCTTCTTCGTCGTCGTCGGACTCTTCCTTcgccgc                                                             <  8:9429/135‑1 (MQ=255)
                                                                              cTGAAGCTGATCGGATACCGCACCGAAGCGTTGGGCTGCGACGACCGGATCCGGGTCGCTCTAGGCCTCATCCTCGTCGTCACCGCGTTCGGATTCTTCTTCGTCGTCGTCGGACTCTTCCTTCGCAGCAgccgcc                                                      >  5:185594/1‑136 (MQ=255)
                                                                               tGAAGCTGTTCGGATACCGCACCGAAGCGTTGGGCTGCGACGACCGGATCCGGGTCGCTCACGGCCTCATCCTCGTCGTCACCGCGTTCGGATTCCTCTTCGTGCTCGTGGGCCTCTTACTTCGCCGAACCGGCCt                                                     >  2:298869/1‑136 (MQ=255)
                                                                                          ggATACCGCACCGAAGCGTTGGGCTGCGACGACCGGATCCGGGTCGCTCTCGGCCTCTTCCTCGTCGTCACCGCTTTCGGATTCTTCTTCGTCGTCGTCGGACTCTTCCTTCGCCGCAGCGGCCGTGGCACCTggg                                          >  6:156811/1‑136 (MQ=255)
                                                                                          ggATACCGCACCGAAGCGTTGGGCTGCGACGACCGGATCCGGGTCGCTCTCGGCCTCTTCCTCGTCGTCACCGCTTTCGGATTCTTCTTCGTAGTCGTCGGACTCTTCCTTCGCCGCCGCGGCCTTGGCACCggg                                           >  7:319946/1‑135 (MQ=255)
                                                                                               ccGCACCGAAGCGTTGGGCTGCGACGACCGGATCCGGGTCGCTCTCGGCCTCTTCCTCGTCGTCACCGCTTTCGGATTCTTCTTCGTCGTCGTCGGACTCTTCCTTCGCCGCAGCGGCCTTGGCACCTGGGATCg                                      <  8:70706/135‑1 (MQ=255)
                                                                                                     cGAAGCGTTGGGCTGCGACGACCGGATCCGGGTCGCTCTCGGCCTCTTCCTCGTCGTCACCGCTTTCGGATTCTTCTTAGTCGTCGTCGGACTCTTCCTTCGCCGCACCGGCCGTGGCACCTGGGATCGGCActtc                               >  6:269715/1‑136 (MQ=255)
                                                                                                           gTTGGGCTGCGACGACCGGATCCGGGTCGCTCTCGGCCTCTTCCTCGTCGTCACCGCTTTCGGATTCTTCTTCGTCGTCGTCGGACTCTTCCTTCGCCGCAGCGGCCTTGGCACCTGGGATCGGCACTTCTTCGGt                         <  7:187982/136‑1 (MQ=255)
                                                                                                                    cgacgaCCGGATCCGGGTCGCTCTCGGCCTCTTCCTCGTCGTCACCGCTTTCGGATTCTTCTTCGTCGTCGTCGGACTCTTCCTTCGCCGCAGCGGCCTTGGCACCTGGGATCGGCACTTCTTCGGTTGGcgcg                  <  4:39133/134‑1 (MQ=255)
                                                                                                                    cgacgaCCGGATCCGGGTCGCTCTCGGCCTCTTCCTCGTCGTCACCGCTTTCGGATTCTTCTTCGTCGTCGTCGGACTCTTCCTTCGCCGCAGCGGCCTTGGCACCTGGGATCGGCACTTCTTCGGTTGGcgcg                  <  8:30825/134‑1 (MQ=255)
                                                                                                                       cgacCGGATCCGGGTCGCTCTCGGCCTCTTCCTCGTCGTCACCGCTTTCGGATTCTTCTTCGTCGTCGTCGGCCTCTTCCTTCGCCGCCGCGGCCTTGGCACCTGGGATCGGCACTTCTTCGGTTGGCGCGGCAAt             >  6:317717/1‑136 (MQ=255)
                                                                                                                            ggATCCGGGTCGCTCTCGGCCTCTTCCTCGTCGTCACCGCGTTCGGATTCTTCTTCGTCGTCGTCGGACTCTTCCTTCGCCGCAGCGGCCGTGGCACCTGGGATCGGCACGTCTTAGGTTGGCGCGGCAAtgttgt        >  7:309776/1‑136 (MQ=255)
                                                                                                                            ggATCCGGGTCGATCTCGGCCTCTTCCTCGTCGTCACCGCGTTCGGATTATTCTTAGTGGTCGTCGGGCTCGTGCTTCGCCGCCGCGGCCTGGGGACCGGGGATCGGGAAGTCGTCGGTGGGCTGGGGAAtgttgt        >  2:263390/1‑136 (MQ=255)
                                                                                                                             gATCCGGGTCGCTCTCGGCCTCTTCCTCGTCGTCACCGCTTTCGGATTCTTCTTCGTCGTCGTCGGACTCTTCCTTCGCCGCAGCGGCCTTGGCACCTGGGATCGGCACTTCTTCGGTTGGCGCGGCAATGTTgtc       <  7:217819/136‑1 (MQ=255)
                                                                                                                                  gggTCGCTCTCGGCCTCTTCCTCGTCGTCACCGCTTTCGGATTCTTCTTCGTCGTCGTCGGACTCTTCCTTCGCCGCAGCGGCCTTGGCACCTGGGATCGGCACTTCTTCGGTTGGCGCGGCAATGTTGTCGTCAg  <  5:202837/136‑1 (MQ=255)
                                                                                                                                  gggTCGCTCTCGGCCTCTTCCTCGTCGTAACCGCTTTCGGATTCTTCTTCGTCGTCGTCGGACTCTTCCTTCGCCGCAGCGGCCTTGGCACCTGGGATCGGCACTTCTTCGGTTGGCGCGGCAATGTTGTCGTCAg  <  6:211214/136‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                    
CAGGTCAGCCAGGGCCTGCAGGGCCTCGATGCTTTCCTTGTGGTTGCGACCATTTTTCTTCAGGACCTTGGAGGTTGCCTGAAGCTGATCGGATACCGCACCGAAGCGTTGGGCTGCGACGACCGGATCCGGGCCGCTCTCGGCCTCTTCCTCGTCGTCACCGCTTTCGGATTCTTCTTCGTCGTCGTCGGACTCTTCCTTCGCCGCAGCGGCCTTGGCACCTGGGATCGGCACTTCTTCGGTTGGCGCGGCAATGTTGTCGTCAG  >  ACB143/465211‑465476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑