Predicted mutation
evidence seq id position mutation annotation gene description
RA ACB143 6,137,970 T→C V171A (GTG→GCG)  cusA → Cation efflux system protein CusA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1436,137,9700TC95.3% 221.3 / 0.7 64V171A (GTG→GCG) cusACation efflux system protein CusA
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base C (34/27);  total (36/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

TCGGGCCGGACGCCACAGGCGTTGGTTGGATCTACCAGTACGCGTTGGTCGACCGCAGTGGTAGGCATGATCTGGCACAACTGCGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGTGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATACCAGGTGGTACTCGACCCGCTGCGCATGGCCAGCCTCGGCATCACCCAGGGCGAGGTCGCAAACGCCATCGGTAAG  >  ACB143/6137844‑6138094
                                                                                                                              |                                                                                                                            
tCGGGCCGGACGCCACAGGCGTTGGTTGGATCTACCAGTACGCGTTGGTCGACCGCAGTGGTAGGCATGATCTGGCACAACTGCGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGt                                                                                                                     >  1:1736/1‑136 (MQ=255)
     ccGGACGCCACAGGCGTTGGTTGGATCTACCAGTACGCGTTGGTCGACCGCAGTGGTAGGCATGATCTGGCACAACTGCGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGcc                                                                                                                 >  4:181780/1‑135 (MQ=255)
         aCGCCACAGGCGTTGGTTGGATCTACCAGTACGCGTTGGTCGACCGCAGTGGTAGGCATGATCTGGCACAACTGCGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGt                                                                                                            <  2:21593/136‑1 (MQ=255)
                 ggCGTTGGTTGGATCTACCAGTACGCGTTGGTCGACCGCAGTGGTAGGCATGATCTGGCACAACTGCGGAGCCTGCAGGATTGGTTCCTGCGCTTTGCGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCa                                                                                                    >  7:4667/1‑136 (MQ=255)
                 ggCGTTGGTTGGATCTACCAGTACGCGTTGGTCGACCGCAGTGGTAGGCATGATCTGGCACAACTGCGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGTGCCGGACGTCGCCGAAGTGGCAAGCa                                                                                                    >  2:4061/1‑136 (MQ=255)
                  gCGTTGGTTGGATCTACCAGTACGCGTTGGTCGACCTCATTGGTAGGCATGATCTGGCACAACTTCGGAGCCTGCAGGATTGGTTCCTTCGCTTTGAGCTCAAGACCGCGCCGTACGTCGCCGAAGTGGCAAGCAt                                                                                                   <  6:315462/136‑1 (MQ=255)
                     ttggttggATCTACCAGTACGCGTTGGTCGACCGCAGTGGTAGGCATGATCTGGCACAACTGCGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTgg                                                                                                <  8:84415/136‑1 (MQ=255)
                     ttggttggATCTACCAGTACGCGTTGGTCGACCGCAGGGGTAGGCATGATCTGGCACAACTGCGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTgg                                                                                                <  8:19459/136‑1 (MQ=255)
                       ggttggATCTACCAGTACGCGTTGGTCGACCGCAGTGGTAGGCATGATCTGGCACAACTGCGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTggc                                                                                               <  7:11758/135‑1 (MQ=255)
                          tggATCTACCAGTACGCGTTGGTCGACCGCAGTGGTAGGCATGATCTGGCACAACTGCGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCa                                                                                           >  4:208086/1‑136 (MQ=255)
                          tggATCTACCAGTACGCGTTGGTCGACCGCAGTGGTAGGCATGATCTGGCACAACTGCGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCa                                                                                           >  3:158878/1‑136 (MQ=255)
                           ggATCTACCAGTACGCGTTGGTCGACCGCAGTGGTAGGCATGATCTGGCACAACTGCGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCa                                                                                           <  1:115011/135‑1 (MQ=255)
                           ggATCTACCAGTACGCGTTGGTCGACCGCAGTGGTAGGCATGATCTGGCACAACTGCGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCa                                                                                           <  7:108978/135‑1 (MQ=255)
                            gATCTACCAGTACGCGTTGGTCGACCGCAGTGGTAGGCATGATCTGGCACAACTGCGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGGCGCCGAAGTGGCAAGCATTGGCGGCAt                                                                                          <  7:118752/135‑1 (MQ=255)
                               cTACCAGTACGCGTTGGTCGACCGCAGTGGTAGGCATGATCTGGCACAACTGCGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGt                                                                                       >  3:15849/1‑135 (MQ=255)
                                  ccAGTACGCGTTGGTCGACCGCAGTGGTAGGCATGATCTGGCACAACTGCGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGtt                                                                                      <  5:39881/133‑1 (MQ=255)
                                  ccAGTACGCGTTGGTCGACCGCAGTGGTAGGCATGATCTGGCACAACTGCGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGtt                                                                                      <  4:254585/133‑1 (MQ=255)
                                  ccAGTACGCGTTGGTCGACCGCAGTGGTAGGCATGATCTGGCACAACTGCGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGtt                                                                                      <  2:84481/133‑1 (MQ=255)
                                    aGTACGCGTTGGTCGACCGCAGTGGTAGGCATGATCTGGCACAACTGCGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCa                                                                                 <  7:328051/136‑1 (MQ=255)
                                     gTACGCGTTGGTCGACCGCAGTGGTAGGCATGATCTGGCACAACTGCGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCaa                                                                                <  1:269008/136‑1 (MQ=255)
                                     gTACGCGTTGGTCGACCGCAGTGGTAGGCATGATCTGGCACAACTGCGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCaa                                                                                >  4:154375/1‑136 (MQ=255)
                                     gTACGCGTTGGTCGACCGCAGTGGTAGGCATGATCTGGCACAACTGCGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCaa                                                                                >  1:357614/1‑136 (MQ=255)
                                       aCGCGTTGGTCGACCGCAGTGGTAGGCATGATCTGGCACAACTGCGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAAt                                                                               <  2:1736/135‑1 (MQ=255)
                                         gcgTTGGTCGACCGCAGTGGTAGGCATGATCTGGCACAACTGCGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATAcc                                                                            <  1:117833/136‑1 (MQ=255)
                                         gcgTTGGTCGACCGCAGTGGTAGGCATGATCTGGCACAACTGCGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATAcc                                                                            <  1:235006/136‑1 (MQ=255)
                                         gcgTTGGTCGACCGCAGTGGTAGGCATGATCTGGCACAACTGCGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATAc                                                                             >  7:190730/1‑135 (MQ=255)
                                         gcgTTGGTCGACCGCAGTGGTAGGCATGATCTGGCACAACTGCGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATAc                                                                             >  5:340995/1‑135 (MQ=255)
                                          cgTTGGTCGACCGCAGTGGTAGGCATGATCTGGCACAACTGCGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATAc                                                                             >  7:55307/1‑134 (MQ=255)
                                           gTTGGTCGACCGCAGTGGTAGGCATGATCTGGCACAACTGCGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGACGTGGCAAGCATTGGCGGCATGGTTAAGCAATACCAg                                                                          >  3:225720/1‑136 (MQ=255)
                                                   aCCGCAGTGGTAGGCATGATCTGGCACAACTGCGGAGCCTGCAGGATTGGTTCCTGCGATTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATACCAGGTGGTACt                                                                  >  5:172404/1‑136 (MQ=255)
                                                                     aTCTGGCACAACTGCGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATACCAGGAGGTACTCGACCCGCTGCGCATgtc                                                >  6:22426/1‑134 (MQ=255)
                                                                       cTGGCACAACTGCGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATACCAGGTGGTCCTCGACCCGCTGCGCATGGc                                                >  3:336983/1‑134 (MQ=255)
                                                                       cTGGCACAACTGCGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATACCAGGTGGTACTCGACCCGCTGCGCATGGc                                                >  1:331596/1‑134 (MQ=255)
                                                                          gCACAACTGCGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATACCAGGTGGTACTCGACCCGCTGCGCATGGCCAg                                             <  3:315232/134‑1 (MQ=255)
                                                                          gCACAACTGCGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATACCAGGTGGTACTCGACCCGCTGCGCATGGCCAg                                             <  6:340995/134‑1 (MQ=255)
                                                                             caACTGCGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATACCAGGTGGTACTCGACCCGCTGCGCATGGCCAGCCTCg                                        <  2:353761/136‑1 (MQ=255)
                                                                             caACTGCGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATACCAGGTGGTACTCGACCCGCTGCGCATGGCCAGCCTCg                                        <  1:338123/136‑1 (MQ=255)
                                                                             caACTGCGGAGCCTGCAGGAGTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATACCAGGTGGTACTCGACCCGCTGCGCATGGCCAGCCTCg                                        <  2:287025/136‑1 (MQ=255)
                                                                              aaCTGCGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATACCAGGTGGTACTCGACCCGCTGCGCATGGCCAGCCTCgg                                       >  4:348412/1‑136 (MQ=255)
                                                                              aaCTGCGGAGCCTGCAGGATTGGGTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATACCAGGTGGTACTCGACCCGCTGCGCATGGCCAGCCTCgg                                       <  6:350702/136‑1 (MQ=255)
                                                                                   cGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATACCAGGTGGTACTCGACCCGCTGCGCAt                                                   >  3:155795/1‑119 (MQ=255)
                                                                                   cGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATACCAGGTGGTACTCGACCCGCTGCGCAt                                                   <  4:155795/119‑1 (MQ=255)
                                                                                   cGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATACCAGGTGGTACTCGACCCGCTGCGCATGGCCAGCCTCGGCATCa                                  >  8:166116/1‑136 (MQ=255)
                                                                                   cGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATACCAGGTGGTACTCGACCCGCTGCGCATGGCCAGCCTCGGCATCa                                  >  7:23523/1‑136 (MQ=255)
                                                                                   cGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATACCAGGTGGTACTCGACCCGCTGCGCATGGCCAGCCTCGGCATCa                                  >  2:118360/1‑136 (MQ=255)
                                                                                      aGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATACCAGGTGGTACTCGACCCGCTGCGCATGGCCAGCCTCGGCATCAcc                                >  6:288728/1‑135 (MQ=255)
                                                                                       gCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATACCAGGTGGTACTCGACCCGCTGCTCATTTCCAGACTCGGCATCACCCa                              <  1:361748/136‑1 (MQ=255)
                                                                                       gCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATACCAGGTGGTACTCGACCCGCTGCGCATGGCCAGCCTCGGCATCAcc                                >  2:28708/1‑134 (MQ=255)
                                                                                        ccTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATACCAGGTGGTACTCGACCCGCTGCGCATGGCCAGCCTCGGCATCACCCAg                             >  1:112277/1‑136 (MQ=255)
                                                                                           gCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGTGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATACCAGGTGGTACTCGACCCGCTGCGCATGGCCAGCCTCGGCATCACCCAGGGc                          >  2:133728/1‑136 (MQ=255)
                                                                                            cAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATACCAGGTGGTACTCGACCCGCTGCGCATGGCCAGCCTCGGCATCACCCAGGGc                          <  7:99323/135‑1 (MQ=255)
                                                                                              ggATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATACCAGGTGGTACTCGACCCGCTGCGCATGGCCAGCCTCGGCATCACCCAGGGCGAg                       >  5:283592/1‑136 (MQ=255)
                                                                                              ggATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATACCAGGTGGTACTCGACCCGCTGCGCATGGCCAGCCTCGGCATCACCCAGGGCGAg                       >  6:16351/1‑136 (MQ=255)
                                                                                               gATTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATACCAGGTGGTACTCGACCCGCTGCGCATGGCCAGCCTCGGCATCACCCAGGGCGAgg                      <  8:160436/136‑1 (MQ=255)
                                                                                                aTTGGTTCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATACCAGGTGGTACTCGACCCGCTGCGCATGGCCAGCCTCGGCATCACCCAGTGAGAGGt                     >  6:8680/1‑136 (MQ=255)
                                                                                                     ttCCTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATACCAGGTGGTACTCGACCCGCTGCGCATGGCCAGCCTCGGCATCACCCAGGGCGAGGTCGCa                 >  2:305189/1‑135 (MQ=255)
                                                                                                     ttCCCGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATACCAGGTGGTACTCGACCCGCTGCGCATGGCCAGCCTCGGCATCACCCAGGGCGAGGTCGCa                 >  1:122236/1‑135 (MQ=255)
                                                                                                       ccTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGTCGGCAGGCTTAAGCAATACCAGGTGGTACTCGACCCGCTGCGCATGGCCAGCCTCGGCATCACCCAGGGCGAGGTCGCaacc              >  8:173716/1‑134 (MQ=255)
                                                                                                       ccTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATACCAGGTGGTACTCGACCCGCTGCGCATGGCCAGCCTCGGCATCACCCAGGGCGAGGTCGCAAAc              >  1:186032/1‑136 (MQ=255)
                                                                                                       ccTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATACCAGGTGGTACTCGACCCGCTGCGCATGGCCAGCCTCGGCATCACCCAGGGCGAGGTCGCAAAc              >  2:313616/1‑136 (MQ=255)
                                                                                                       ccTGCGCTTTGAGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATACCAGGTGGTACTCGACCCGCTGCGCATGGCCAGCCTCGGCATCACCCAGGGCGAGGTCGCAAAc              >  8:305480/1‑136 (MQ=255)
                                                                                                        cTGCGCTTTGAGCTCAAGACCGTGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATACCAGGTGGTACTCGACCCGCTGCGCATGGCCAGCCTCGGCATCACCCAGGGCGAGGTCGCAAAc              <  1:4061/135‑1 (MQ=255)
                                                                                                                  aGCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATACCAGGTGGTACTCGACCCGCTGCGCATGGCCAGCCTCGGCATCACCCAGGGCGAGGTCGCAAACGCCATCGGt     >  1:94229/1‑134 (MQ=255)
                                                                                                                   gCTCAAGACCGCGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATACCAGGTGGTACTCGACCCGCTGCGCATGGCCAGCCTCGGCATCACCCAGGGCGAGGTCGCAAACGCCATCGGTAAg  <  2:88111/136‑1 (MQ=255)
                                                                                                                              |                                                                                                                            
TCGGGCCGGACGCCACAGGCGTTGGTTGGATCTACCAGTACGCGTTGGTCGACCGCAGTGGTAGGCATGATCTGGCACAACTGCGGAGCCTGCAGGATTGGTTCCTGCGCTTTGAGCTCAAGACCGTGCCGGACGTCGCCGAAGTGGCAAGCATTGGCGGCATGGTTAAGCAATACCAGGTGGTACTCGACCCGCTGCGCATGGCCAGCCTCGGCATCACCCAGGGCGAGGTCGCAAACGCCATCGGTAAG  >  ACB143/6137844‑6138094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: