Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	ACB143	1,227,173	A→G	V389A (GTC→GCC)	glpK ←	Glycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	1,227,173	0	A	G	95.9%	259.1 / 0.6	74	V389A (GTC→GCC)	glpK	Glycerol kinase
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base G (37/33);  total (38/36)
Fisher's exact test for biased strand distribution p-value = 6.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01

ATCTGCGGCCGCTCCACGCAGGTGCCGAGGATGTCGGCCTGGAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGACATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCATACGGGTCCCAGTACGGGGCACCCAGGCCGGT  >  ACB143/1227041‑1227300                                                                                                                                     |                                                                                                                                aTCTGCGGCCGCTCCACGCAGGTGCCGAGGATGTCGGCCTGGAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCAt                                                                                                                              >  1:214368/1‑136 (MQ=255) aTCTGCGGCCGCTCCACGCAGGTGCCGAGGATGTCGGCCTGGAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGACAt                                                                                                                              <  4:93408/136‑1 (MQ=255)      cGGCCGCTCCACGCAGGTGCCGAGGATGTCGGCCTGGAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATcg                                                                                                                            <  1:86135/133‑1 (MQ=255)        gCCGCTCCACGCAGGTGCCGAGGATGTCGGCCTGGAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGGCGAGGGCATCGCGGGt                                                                                                                       <  6:173648/136‑1 (MQ=255)              ccACGCAGGTGCCGAGGATGTCGGCCTGGAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTgg                                                                                                                   >  4:329876/1‑134 (MQ=255)               cACTCAGGTGCCGAGTATGTCGGCCTGTAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGt                                                                                                                  <  5:68466/134‑1 (MQ=255)                  gCAGGTGCCGAGGATGTCGGCCTGGAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCg                                                                                                             <  4:215387/136‑1 (MQ=255)                  gCAGGTGCCGAGGATGTCGGCCTGGAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCg                                                                                                             <  1:17561/136‑1 (MQ=255)                  gCAGGTGCCGAGGATGTCGGCCGGGAACTGCATGAGGCAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGGCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCg                                                                                                             <  5:26353/136‑1 (MQ=255)                         ccGAGGATGTCGGCCTGGAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACt                                                                                                      >  5:43171/1‑136 (MQ=255)                          cGAGGATGTCGGCCTGGAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTc                                                                                                     >  7:128753/1‑136 (MQ=255)                            aGGATGTCGGCCTGGAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTc                                                                                                     >  7:204809/1‑134 (MQ=255)                               aTGTCGGCCTGGAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTg                                                                                                >  6:3169/1‑136 (MQ=255)                                          taaCTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGgatg                                                                                     <  8:337595/135‑1 (MQ=255)                                          gAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGgatg                                                                                     <  7:47724/136‑1 (MQ=255)                                          gAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGgat                                                                                      <  8:57919/135‑1 (MQ=255)                                             cTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGAGCGGCGCGGaggatg                                                                                  >  4:150435/1‑136 (MQ=255)                                                cATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGGAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGGGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGAtgtg                                                                                <  2:186882/135‑1 (MQ=255)                                                cATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGTCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGAtgtg                                                                                <  8:258538/135‑1 (MQ=255)                                                cATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGAtgtg                                                                                <  1:133334/135‑1 (MQ=255)                                                cATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGAtgtg                                                                                <  1:340371/135‑1 (MQ=255)                                                cATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTgg                                                                               <  5:56438/136‑1 (MQ=255)                                                   gAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTTGTc                                                                             >  8:128926/1‑135 (MQ=255)                                                   gAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTc                                                                             >  7:178272/1‑135 (MQ=255)                                                   gAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTc                                                                             >  4:263459/1‑135 (MQ=255)                                                   gAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCt                                                                            >  6:47044/1‑136 (MQ=255)                                                             ttgGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTCCCTTCACGc                                                                  >  1:55198/1‑136 (MQ=255)                                                             ttgGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGc                                                                  >  5:344104/1‑136 (MQ=255)                                                             ttgGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGc                                                                  >  5:148423/1‑136 (MQ=255)                                                             ttgGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGc                                                                  >  8:174661/1‑136 (MQ=255)                                                                gCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCAc                                                               <  3:141723/136‑1 (MQ=255)                                                                  cACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACgt                                                             >  5:66489/1‑136 (MQ=255)                                                                     ggCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTc                                                          <  2:62310/136‑1 (MQ=255)                                                                          caccGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGcc                                                     <  5:56150/136‑1 (MQ=255)                                                                                  aCGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACg                                             >  1:166460/1‑136 (MQ=255)                                                                                  aCGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACg                                             >  3:154227/1‑136 (MQ=255)                                                                                  aCGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACg                                             >  2:85161/1‑136 (MQ=255)                                                                                  aCGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATCTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACg                                             >  1:269150/1‑136 (MQ=255)                                                                                    gcgcAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGca                                           >  4:99686/1‑136 (MQ=255)                                                                                    gcgcAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGca                                           <  4:209143/136‑1 (MQ=255)                                                                                    gcgcAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGca                                           >  7:235430/1‑136 (MQ=255)                                                                                    gcgcAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGca                                           >  3:61297/1‑136 (MQ=255)                                                                                      gcAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGcacc                                         >  3:147099/1‑136 (MQ=255)                                                                                             ggACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACggg                                    <  1:198634/134‑1 (MQ=255)                                                                                             ggACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCa                                  <  8:259122/136‑1 (MQ=255)                                                                                             ggACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGACATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCa                                  >  3:357205/1‑136 (MQ=255)                                                                                              gACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCAt                                 <  1:172023/136‑1 (MQ=255)                                                                                              gACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCAt                                 <  4:28607/136‑1 (MQ=255)                                                                                              gACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCAt                                 <  4:153318/136‑1 (MQ=255)                                                                                              gACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCAt                                 <  6:60477/136‑1 (MQ=255)                                                                                                 aGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCATACg                              <  1:142156/136‑1 (MQ=255)                                                                                                 aGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCATACg                              <  3:215446/136‑1 (MQ=255)                                                                                                 aGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCATACg                              <  1:295890/136‑1 (MQ=255)                                                                                                   gcgcTGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCATACggg                            <  2:77214/136‑1 (MQ=255)                                                                                                   gcgcTGGCCACAGTCCTGCTGCATGGCGTCGAGGACATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCATACggg                            <  2:105801/136‑1 (MQ=255)                                                                                                       tGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTg                                                                                                                    <  6:288906/44‑1 (MQ=255)                                                                                                       tGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTg                                                                                                                    >  5:288906/1‑44 (MQ=255)                                                                                                       tGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCATACGGGTcc                         >  1:70345/1‑135 (MQ=255)                                                                                                       tGGCCACAGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCATACGGGTcc                         >  1:12035/1‑135 (MQ=255)                                                                                                           cacaGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGCTGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCATACGGGTCCCAGTa                    >  4:196193/1‑136 (MQ=255)                                                                                                            acaGTCCTGCTGCATGGCGTCTAGGGCATCGAGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCATACGGGTCACAGTAc                   >  7:29207/1‑136 (MQ=255)                                                                                                            acaGTCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCATACGGGTCCCAGTAc                   >  5:53172/1‑136 (MQ=255)                                                                                                                tCCTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGAGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCAGAACAACGCACCACGGGCATACGGGTCCCAGTACgggg               >  8:5190/1‑136 (MQ=255)                                                                                                                 ccTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCATACGGGTCCCAGTACgggg               >  2:100530/1‑135 (MQ=255)                                                                                                                 ccTGCTGCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACCTGTCAGGCCGAACCACGCACCACGGGCATACGGGTCCCAGTACGGGGc              >  7:181201/1‑136 (MQ=255)                                                                                                                  ctgctgCATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCATACGGGTCCCAGTACGGGGc              <  6:329601/135‑1 (MQ=255)                                                                                                                     ctgcATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCATACGGGTCCCAGTACGGGGCAccc          <  7:100593/136‑1 (MQ=255)                                                                                                                     ctgcATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCATACGGGTCCCAGTACGGGGCAccc          <  6:327418/136‑1 (MQ=255)                                                                                                                     ctgcATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCATACGGGTCCCAGTACGGGGCAccc          <  4:154227/136‑1 (MQ=255)                                                                                                                       gcATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCATACGGGTCCCAGTACGGGGCACCCAg        >  1:319133/1‑136 (MQ=255)                                                                                                                        cATGGCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCATACGGGTCCCAGTACGGGGCACCCAgg       >  8:66660/1‑136 (MQ=255)                                                                                                                            gCGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCATACGGGTCCCAGTACGGGGCACCCAGGCCg    <  1:33078/135‑1 (MQ=255)                                                                                                                             cGTCGAGGGCATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCATACGGGTCCCAGTACGGGGCACCCAGGCCgg   >  2:353419/1‑135 (MQ=255)                                                                                                                             cGTCGAGGGCATCGC‑GGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCATACGGGTCCCAGTACGGGGCACCCAGGCCGGt  >  7:35073/1‑135 (MQ=255)                                                                                                                                     |                                                                                                                                ATCTGCGGCCGCTCCACGCAGGTGCCGAGGATGTCGGCCTGGAACTGCATGAGGAAGTTGTTGGCCACGGCACCACCGTCCACGCGCAGCTCGGACAGGCGCTGGCCACAGTCCTGCTGCATGGCGTCGAGGACATCGCGGGTCTGGTAGGCGATGGACTCCAGTGCGGCGCGGATGATGTGGTCTACCTTCACGCCACGTGTCAGGCCGAACAACGCACCACGGGCATACGGGTCCCAGTACGGGGCACCCAGGCCGGT  >  ACB143/1227041‑1227300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑