Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1433,440,3590CG40.0% 22.1 / 11.4 30Q29E (CAG→GAG) CJ019_03266hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (9/9);  new base G (12/0);  total (21/9)
Fisher's exact test for biased strand distribution p-value = 4.04e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGG‑CCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGATCAGCTGGCTGAAGAACT  >  ACB143/3440225‑3440495
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ggTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCACCGCTGGCGGCGCCCGGGCCGCCGGGGTGTGGccg                                                                                                                                         >  1:221203/1‑135 (MQ=255)
       aggaTCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAAcc                                                                                                                                  >  5:240260/1‑136 (MQ=255)
          aTCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGcca                                                                                                                               >  6:243026/1‑136 (MQ=255)
              cAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCGGCCCGCGCTGGCGGCGCCCGGGCCGCCGGGGTGTGGGGGGCCCACGccgagaa                                                                                                                           >  1:296640/1‑132 (MQ=255)
                         aCAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGGGTGGCAGGCCCACGCCGATAAGGCGCAGCTCg                                                                                                                >  1:128213/1‑137 (MQ=255)
                           aaGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCCCTGGCGGCGCCCCGGCACCCGGGGGGTGGCAGGCCCACCCCCAAAAGGCGCAGCTCGcg                                                                                                              >  3:47856/1‑136 (MQ=255)
                           aaGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCCCCAGCGCTGGCGGCGCCCGGGCCGCCGGGGGGGGGGCGGCCACCGCCAATAAGGACCAGCTCGcg                                                                                                              >  3:47857/1‑136 (MQ=255)
                                   gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGGGGCGCCCCGGCCGCCGGGGTGTGGCGGGCCACCCCCTATAATGAGCCGCTCGCGGCTAAGG‑c                                                                                                     >  3:48136/1‑137 (MQ=255)
                                   gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCGGGGTGTGGCAGGCCAACGCCGATAAGGCGCAGCTCGCTGATCAGG‑c                                                                                                     >  7:293935/1‑137 (MQ=255)
                                   gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCCGGCCGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCGGCTCAGG‑c                                                                                                     >  2:96065/1‑137 (MQ=255)
                                   gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCACCGCTGGCGGCGCCCCGGCAGCCTGGGTGGGGGAGGCCACCGCCTATAAGGCGCAGATCCCCGATAAGG‑c                                                                                                     >  6:115075/1‑137 (MQ=255)
                                   gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCCCCCGCGCTGGCGGCGCCCGGGCCGCCTGGGTGTGGGAGGCCAACGCCTATAAGGCGCCGCTCGCGGATCAGG‑c                                                                                                     >  4:206946/1‑137 (MQ=255)
                                     tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCGGGGTGTGGGCGGCCAACGCCGATAAGGCGCAGCTCGCTGATCAGG‑CCg                                                                                                   >  7:30078/1‑137 (MQ=255)
                                     tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCGGGGGTGTGGGAGGCCCCCGCCGAGAAGGCGCCGCTCGCGGAGCAGG‑ccc                                                                                                   >  6:282849/1‑136 (MQ=255)
                                     tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCGGGGGGCGCCCGGGCCGCCGGGGTGTGGGCGGCCCACGCCTATAAGGCGCAGCGCGCTGATCAGG‑CCg                                                                                                   >  4:226642/1‑137 (MQ=255)
                                       gAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGGGCCCGGGGCGCCGGGGGGTGGGCGGCCCACGCCGATAAGGCGCCGCTCGCGGCTGAGG‑CCgcg                                                                                                 >  1:244495/1‑135 (MQ=255)
                                                        gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCGGGGGGTGGGCGGCCACCGCCGATAAGGCGCCGCGCGCGGATCAGG‑CCGATGACTTCGGCAAGCAGtt                                                                                >  5:283085/1‑137 (MQ=255)
                                                        gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCCGGCCGCCTGGGTGTGGCAGGCCCACGCCTATAAGGCGCCGCTCGCGGATCAGG‑CCGCTGACGTCGGCCAGCAGtt                                                                                >  8:170963/1‑137 (MQ=255)
                                                        gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCAGCCGGGGGGTGGGCCGCCCACCCCTATAAGGCGCCGCACGCGGCTCAGGCCCGAT‑CCGTCGGCAAGCAggt                                                                                >  8:271464/1‑135 (MQ=255)
                                                        gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGCGGCGCCCGGGCCCCCGGGGTGTGGGAGGCCAACGCCGATAAAGCGCAGCGCGCTGCTGCGG‑CCGATGACTTGGGCCAGCAGtt                                                                                >  2:95807/1‑137 (MQ=255)
                                                             ggCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCCGGCCGCCGGGGGGTGGGAGGGCCACCCCGATAAGGCGCAGCGCGCGGGTCAGG‑CCGCTGCCTTGGGCCAGCAGGTGgggg                                                                           >  8:278861/1‑137 (MQ=255)
                                                                  cccGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCGGGGTGTGGCAGGCCCACGCCGATAAGGCGCAGCGCGCGGCGCAGG‑CCGAGGACGTCGTCCAGCAGGTGGCGGAGaaa                                                                      >  8:160760/1‑136 (MQ=255)
                                                                          gggggggTGGCGCTGGGGCGGCCCGCCCGGGGGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGG‑CCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc                                                              <  5:243026/131‑1 (MQ=255)
                                                                                            ggggCCGCCTGGGGGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCTCTGATCAGG‑CCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGga                                            <  5:207121/135‑1 (MQ=255)
                                                                                            cgggCCGCCCGCGCGGGCCGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGG‑CCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCACGCGCACGGTCTGGAGCGGga                                            <  8:30078/135‑1 (MQ=255)
                                                                                                       ggggggcggcGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGG‑CCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTgcag                                 <  6:283085/133‑1 (MQ=255)
                                                                                                           ggcgccGCGCGGGCAGGCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGG‑CCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTgcagcagc                             <  3:38093/137‑1 (MQ=255)
                                                                                                                cgcCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGG‑CCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAgcgc                        <  2:304220/137‑1 (MQ=255)
                                                                                                                       gCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGG‑CCTATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTTGAGCGGGAGAAGGCTTCAGCAGCAGCGCTCGATCa                 <  1:67988/137‑1 (MQ=255)
                                                                                                                       gCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGG‑CCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGATCa                 <  7:170963/137‑1 (MQ=255)
                                                                                                                             tGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGG‑CCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGATCAgctggc           <  5:300849/137‑1 (MQ=255)
                                                                                                                                      cAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGG‑CCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGATCAGCTGGCTGAAGAACt  >  7:307134/1‑137 (MQ=255)
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GGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGG‑CCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGATCAGCTGGCTGAAGAACT  >  ACB143/3440225‑3440495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑