| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | ACB143 | 5,386,500 | C→A | intergenic (‑509/+1142) | CJ019_05070 ← / ← nptII | hypothetical protein/neomycin phosphotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | ACB143 | 5,386,500 | 0 | C | A | 93.8% | 38.8 / ‑6.4 | 16 | intergenic (‑509/+1142) | CJ019_05070/nptII | hypothetical protein/neomycin phosphotransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (7/8); total (7/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAGCCAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGT‑‑CGT‑CA‑GGAT‑C‑‑A‑‑TCCGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTT > ACB143/5386369‑5386602 | tAGCCAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagt‑‑ggt < 4:229796/137‑1 (MQ=255)tAGCCAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagt‑‑ggt < 7:250829/137‑1 (MQ=255)tAGCCAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagt‑‑ggt < 5:69430/137‑1 (MQ=255) aGCCAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagt‑‑ggtg < 2:296677/137‑2 (MQ=255) ccAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagt‑‑ggtgta > 2:75219/1‑134 (MQ=255) gcagTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCagt‑‑ggtgtacgg > 8:133162/1‑131 (MQ=255) tATTTATAAAAAAAATTGATCTAATTCTTTCATTTAATACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGGCTCAGT‑‑GGTGTACGGTA‑C‑‑AACCCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGaa < 4:72639/137‑1 (MQ=255) tATTGAAAACAAAAATTGCTCGAATTCTTTCATTTAAGACCCTAATCTGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTA‑CAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGaa < 3:118565/137‑1 (MQ=255) aaCAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTA‑CAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCAcc > 2:78492/1‑137 (MQ=255) aaaaaaTTGATCTAATTCTTTCATTTAAGACCCTAATATGACAGCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGGGT‑AC‑GGTA‑CAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCta < 5:281863/136‑2 (MQ=255) aaaaaTTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTA‑C‑‑AACCCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCAGCttg > 6:241103/1‑136 (MQ=255) aaTTGATCGACTTCTTTCATTTAAGACCCAAATATGACATCGCGGCCGGCTTGTCGACGACGGCGGTCTCAGG‑‑GGTGTACGGTA‑CAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCgg < 1:233075/137‑1 (MQ=255) gtatCATTTAACACACTAATATGACATCACGGCCGGCTTGTCGACGACGGCGGTCTCCGT‑‑GGTGTA‑CGTTACAAA‑‑CCCCGACGCTAGCTACGCTTGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTAtt < 3:214805/134‑1 (MQ=255) cATTAAAGCCCCTAATATGACATCTCGGCCGGCTTGTCGCCGACGGCGGTCTCAGT‑‑GGTGTACGGTA‑CAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcgc < 6:190059/137‑1 (MQ=255) aaTTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGTGGTGT‑CC‑GGTA‑CAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTgcgc < 8:263231/136‑1 (MQ=255) aGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTA‑CAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCggg < 2:12479/137‑1 (MQ=255) aaTATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTA‑CAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAgg < 5:34780/137‑1 (MQ=255) ctcGGCCGGCTTGTCGACGCCGGCGGTCTCAGT‑‑GGTGTACGGTA‑CAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt > 7:254349/1‑137 (MQ=255) ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTA‑CAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt > 6:238825/1‑137 (MQ=255) ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTA‑CAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCtt > 5:166838/1‑137 (MQ=255) ctcGGCCGGCTTGTCGACGACGGCGGTCTCAGT‑‑GGTGTACGGTA‑CAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGCAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGTTGCCAGGGCGTGCCCtt > 4:296192/1‑137 (MQ=255) atcGGCCGGCTTGTCGACGACTGCGGTCTCAGT‑‑GGTGTACGGTA‑CAAA‑‑CCCCGACGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCTCTGCGTGTGCCAGGGCGTGCCCtt > 2:84756/2‑137 (MQ=255) | TAGCCAGCAGTCTAACTAAAAAGGCCTCCCAAATCGGGGGGCCTTTTTTATTGATAACAAAAATTGATCGAATTCTTTCATTTAAGACCCTAATATGACATCTCGGCCGGCTTGTCGACGACGGCGGTCTCCGT‑‑CGT‑CA‑GGAT‑C‑‑A‑‑TCCGGGCGCTAGCAACGCATGAGAAAGCCCCCGGAAGATCACCTTCCGGGGGCTTTTTTATTGCGCTGCGGGTGCCAGGGCGTGCCCTT > ACB143/5386369‑5386602 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |