Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 1,311,108 Δ1 bp 26.6% coding (1473/1989 nt) aaeB_2 → p‑hydroxybenzoic acid efflux pump subunit AaeB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1431,311,1080A.26.6% 167.6 / 63.1 64coding (1473/1989 nt)aaeB_2p‑hydroxybenzoic acid efflux pump subunit AaeB
Reads supporting (aligned to +/- strand):  ref base A (36/11);  new base . (13/4);  total (49/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01

CGCGTACCGGGGCTACGGCCACTTCCTATTGCCTGCACTTCATTGTGCTGGTGTCGCCGCTCAACGCCATGCAGTTCGGCGTGGCGACCATGCTGAACAGTGCCCTGGCCATGCTGGTGGGGGTGTCGGCAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGTGCTGCAACGCAGAACGACCTGGTGCGCCTGACTCGGCGCGACCTGCGCGGGGCTGACAGCTGGTTTGGCGG  >  ACB143/1310978‑1311242
                                                                                                                                  |                                                                                                                                      
cgcgTACCGGGGCTACGGCCACTTCCTATTGCCTGCACTTCATTGTGCTGGTGTCGCCGCTCAACGCCATGCAGTTCGGCGTGGCGACCATGCTGAACAGTGCCCTGGCCATGCTGGTGGGGGTGTCGGCAGCGGt                                                                                                                                   >  2:25389/1‑136 (MQ=255)
 gcgTACCGGGGCTACGGCCACTTCCTATTGCCTGCACTTCATTGTGCTGGTGTCGCCGCTCAACGCCATGCAGTTCGGCGTGGCGACCATGCTGAACAGTGCCCTGGCCATGCTGGTGGGGGTGTCGGCAGCGGt                                                                                                                                   >  4:400010/1‑135 (MQ=255)
 gcgTACCGGGGCTACGGCCACTTCCTATTGCCTGCACTTCATTGTGCTGGTGTCGCCGCTCAACGCCATGCAGTTCGGCGTGGCGACCATGCTGAACAGTGCCCTGGCCATGCTGGTGGGGGTGTCGG‑‑GCGGTCAt                                                                                                                                >  5:135652/1‑136 (MQ=255)
     aCCGGGGCTACGGCCACTTCCTATTGCCTGCACTTCATTGTGCTGGTGTCGCCGCTCAACGCCATGCAGTTCGGCGTGGCGACCATGCTGAACAGTGCCCTGGCCATGCTGGTGGGGGTGTCGG‑‑GCGGTCATGGcc                                                                                                                            >  4:149965/1‑136 (MQ=255)
       cGGGGCTACGGCCACTTCCTATTGCCTGCACTTCATTGTGCTGGTGTCGCCGCTCAACGCCATGCAGTTCGGCGTGGCGACCATGCTGAACAGTGCCCTGGCCATGCTGGTGGGGGTGTCGGCAGCGGTCATGGcc                                                                                                                            >  3:335059/1‑136 (MQ=255)
       cGGGGCTACGGCCACTTCCTATTGCCTGCACTTCATTGTGCTGGTGTCGCCGCTCAACGCCATGCAGTTCGGCGTGGCGACCATGCTGAACAGTGCCCTGGCCATGCTGGTGGGGGTGTCGGCAGCGGTCATGGcc                                                                                                                            >  7:93376/1‑136 (MQ=255)
               cGGCCACTTCCTATTGCCTGCACTTCATTGTGCTGGTGTCGCCGCTCAACGCCATGCAGTTCGGCGTGGCGACCATGCTGAACAGTGCCCTGGCCATGCTGGTGGGGGTGTCGGCAGCGGTCATGGCCTTTCGCt                                                                                                                     <  4:49460/135‑1 (MQ=255)
                       tCCTATTGCCTGCACTTCATTGTGCTGGTGTCGCAGCTCAACGCCATGCAGTTCGGCGTGGCGACCATGCTGAACAGTGCCCTGGCCATGCTGGTGGGGGTGTCGGCAGCGGTCATGGCCTTTCGCTTGCTGgtgt                                                                                                            >  2:89060/1‑136 (MQ=255)
                                   cACTTCATTGTGCTGGTGTCGCCGCTCAACGCCATGCAGTTCGGCGTGGCGACCATGCTGAACAGTGCCCTGGCCATGCTGGTGGGGGTGTCGG‑‑GCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGcc                                                                                              >  3:342492/1‑136 (MQ=255)
                                      ttCATTGTGCTGGTGTCGCCGCTCAACGCCATGCAGTTCGGCGTGGCGACCATGCTGAACAGTGCCCTGGCCATGCTGGTGGGGGTGTCGGCAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCct                                                                                             >  1:230243/1‑136 (MQ=255)
                                      ttCATTGTGCTGGTGTCGCCGCTCAACGCCATGCAGTTCGGCGTGGCGACCATGCTGAACAGTGCCCTGGCCATGCTGGTGGGGGTGTCGGCAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCct                                                                                             >  5:134079/1‑136 (MQ=255)
                                      ttCATTGTGCTGGTGTCGCCGCTCAACGCCATGCAGTTCGGCGTGGCGACCATGCTGAACAGTGCCCTGGCCATGCTGGTGGGGGTGTCGG‑‑GCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCctgg                                                                                           >  3:222174/1‑136 (MQ=255)
                                      ttCATTGTGCTGGTGTCGCCGCTCAACGCCATGCAGTTCGGCGTGGCGACCATGCTGAACAGTGCCCTGGCCATGATGGTGGGCGTGTCGG‑‑GCGGTCATTGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCctgc                                                                                           >  8:131031/1‑135 (MQ=255)
                                               cTGGTGTCGCCGCTCAACGCCATGCAGTTCGGCGTGGCGACCATGCTGAACAGTGCCCTGGCCATGCTGGTGGGGGTGTCGGCAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGcc                                                                                    >  7:254061/1‑136 (MQ=255)
                                                  gtgtCGCCGCTCAACGCCATGCAGTTCGGCGTGGCGACCATGCTGAACAGTGCCCTGGCCATGCTGGTGGGGGTGTCGGCAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGgc                                                                                 >  3:388789/1‑136 (MQ=255)
                                                   tgtCGCCGCTCAACGCCATGCAGTTCGGCGTGGCGACCATGCTGAACAGTGCCCTGGCCATGCTGGTGGGGGTGTCGGCAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGgcg                                                                                <  6:151261/136‑1 (MQ=255)
                                                     tCGCCGCTCAACGCCATGCAGTTCGGCGTGGCGACCATGCTGAACAGTGCCCTGGCCATGCTGGTGGGGGTGTCGGCAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCt                                                                              >  7:147533/1‑136 (MQ=255)
                                                     tCGCCGCTCAACGCCATGCAGTTCGGCGTGGCGACCATGCTGAACAGTGCCCTGGCCATGCTGGTGGGGGTGTCGGCAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCt                                                                              >  4:139153/1‑136 (MQ=255)
                                                     tCGCCGCTCAACGCCATGCAGTTCGGCGTGGCGACCATGCTGAACAGTGCCCTGGCCATGCTGGTGGGGGTGTCGG‑‑GCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTg                                                                            >  3:33461/1‑136 (MQ=255)
                                                      cgccgcGCAACGCCATGCAGTGCGGCGTGGCGACCATGCTGAACAGTGCCCTGGCCATGCTGGTGGGGGGGTCGGCAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCtt                                                                             <  4:114583/136‑1 (MQ=255)
                                                         cgcTCAACGCCATGCAGTTCGGCGTGGCGACCATGCTGAACAGTGCCCTGGCCATGCTGGTGGGGGTGTCGG‑‑GCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGtg                                                                        <  8:264316/136‑1 (MQ=255)
                                                          gcTCAACGCCATGCAGTTCGGCGTGGCGACCATGCTGAACAGTGCCCTGGCCATGCTGGTGGGGGTGTCGGCAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGt                                                                         >  1:102462/1‑136 (MQ=255)
                                                          gcTCAACGCCATGCAGTTCGGCGTGGCGACCATGCTGAACAGTGCCCTGGCCATGCTGGTGGGGGTGTCGG‑‑GCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGtgc                                                                       >  7:369911/1‑136 (MQ=255)
                                                            tCAACGCCATGCAGTTCGGCGTGGCGACCATGCTGAACAGTGCCCTGGCCATGCTGGTGGGGGTGTCGGCAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGtgc                                                                       >  4:129312/1‑136 (MQ=255)
                                                                cGCCATGCAGTTCGGCGTGGCGACCATGCTGAACAGTGCCCTGGCCATGCTGGTGGGGGTGTCGGCAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGTGCTGCa                                                                   <  1:388582/136‑1 (MQ=255)
                                                                   cATGCAGTTCGGCGTGGCGACCATGCTGAACAGTGCCCTGGCCATGCTGGTGGGGGTGTCGG‑‑GCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGTGCTGCAACGCa                                                              <  2:290068/136‑1 (MQ=255)
                                                                    aTGCAGTTCGGCGTGGCGACCATGCTGAACAGTGCCCTGGCCATGCTGGTGGGGGTGTCGGCAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGTGCTGCAACGc                                                               <  4:326876/136‑1 (MQ=255)
                                                                        aGTTCGGCGTGGCGACCATGCTGAACAGTGCCCTGGCCATGCTGGTGGGGGTGTCGGCAGCGGTCATGTCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGTGCTGCAACGCAGaa                                                           >  1:275653/1‑136 (MQ=255)
                                                                        aGTTCGGCGTGGCGACCATGCTGAACAGTGCCCTGGCCATGCTGGTGGGGGTGTCGGCAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGTGCTGCAACGCAGaa                                                           >  4:391812/1‑136 (MQ=255)
                                                                             ggCGTGGCGACCATGCTGAACAGTGCCCTGGCCATGCTGGTGGGGGTGTCGG‑‑GCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGTGCTGCAACGCAGAACGACCTg                                                    >  5:53256/1‑136 (MQ=255)
                                                                             ggCGTGGCGACCATGCTGAACAGTGCCCTGGCCATGCTGGTGGGGGTGTCGG‑‑GCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGTGCTGCAACGCAGAACGACCTg                                                    >  8:363793/1‑136 (MQ=255)
                                                                              gCGTGGCGACCATGCTGAACAGTGCCCTGGCCATGCTGGTGGGGGTGTCGGCAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGTGCTGCAACGCAGAACGACCt                                                     <  5:391626/136‑1 (MQ=255)
                                                                                  ggCGACCATGCTGAACAGTGCCCTGGCCATGCTGGTGGGGGTGTCGGCAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGTGCTGCAACGCAGAACGACCTGGTg                                                 >  5:293822/1‑136 (MQ=255)
                                                                                       ccATGCTGCACAGGGCCCTGGCCATGCTGGTGGGGGTGTCGGCAGCGGGCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGTGCTGCAACGCAGAACGACCTGGTGCGCCt                                            <  7:121352/136‑1 (MQ=255)
                                                                                       ccATGCTGAACAGTGCCCTGGCCATGCTGGTGGGGGTGTCGGCAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGTGCTGCAACGCAGAACGACCTGGTGCGCCt                                            <  1:205053/136‑1 (MQ=255)
                                                                                       ccATGCTGAACAGTGCCCTGGCCATGCTGGTGGGGGTGTCGG‑‑GCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGTGCTGCAACGCAGAACGACCTGGTGCGCCTGa                                          >  3:397683/1‑136 (MQ=255)
                                                                                           gCTGAACAGTGCCCTGGCCATGCTGGTGGGGGTGTCGGCAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGTGCTGCAACGCAGAACGACCTGGTGCGCCTGACt                                        >  8:301064/1‑136 (MQ=255)
                                                                                            cTGAACAGTGCCCTGGCCATGCTGGGGGGGGGGGCGG‑‑GCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCGGGGCCGGCGCTTGCGTGCTGCAACGCAGAACGACCTGGTGCGCCTGACTCg                                      <  5:347255/135‑1 (MQ=255)
                                                                                             tGAACAGTGCCCTGGCCATGCTGGTGGGGGTGTCGGCAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGTGCTGCAACGCAGAACGACCTGGTGCGCCTGACTCg                                      >  6:337644/1‑136 (MQ=255)
                                                                                                    tGCCCTGGCCATGCTGGTGGGGGTGTCGG‑‑GCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGTGCTGCAACGCAGAACGACCTGGTGCGCCTGACTCGGCGCGACCt                             <  7:15090/136‑1 (MQ=255)
                                                                                                     gCCCTGGCCATGCTGGTGGGGGTGTCGGCAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGTGCTGCAACGCAGAACGACCTGGTGCGCCTGACTCGGCGCGAcc                              <  2:174618/136‑1 (MQ=255)
                                                                                                       ccTGGCCATGCTGGTGGGGGTGTCGGCAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGTGCTGCAACGCAGAACGACCTGGTTCGCCTGACTCGGCGCGACCTg                            >  6:19401/1‑136 (MQ=255)
                                                                                                       ccTGGCCATGCTGGTGGGGGTGTCGGCAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGTGCTGCAACGCAGAACGACCTGGTGCGCCTGACTCGGCGCGCCCTg                            >  2:274848/1‑136 (MQ=255)
                                                                                                       ccTGGCCATGCTGGTGGGGGTGTCGGCAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGTGCTGCAACGCAGAACGACCTGGTGCGCCTGACTCGGCGCGACCTg                            >  7:67572/1‑136 (MQ=255)
                                                                                                       ccTGGCCATGCTGGTGGGGGTGTCGGCAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGTGCTGCAACGCAGAACGACCTGGTGCGCCTGACTCGGCGCGACCTg                            >  2:34931/1‑136 (MQ=255)
                                                                                                       ccTGGCCATGCTGGTGGGGGTGTCGG‑‑GCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGTGCTGCAACGCAGAACGACCTGGTGCGCCTGACTCGGCGCGACCTgcg                          >  5:198020/1‑136 (MQ=255)
                                                                                                         tGGCCATGCTGGTGGGGGTGTCGGCAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGTGCTGCAACGCAGAACGACCTGGTGCGCCTGACTCGGCGCGACCTgcg                          >  4:174964/1‑136 (MQ=255)
                                                                                                         tGGCCATGCTGGTGGGGGTGTCGGCAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGTGCTGCAACGCAGAACGACCTGGTGCGCCTGACTCGGCGCGACCTgcg                          >  1:125432/1‑136 (MQ=255)
                                                                                                         tGGCCATGCTGGTGGGGGTGTCGGCAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGTGCTGCAACGCAGAACGACCTGGTGCGCCTGACTCGGCGCGACCTgcg                          >  5:189938/1‑136 (MQ=255)
                                                                                                         tGGCCATGCTGGTGGGGGTGTCGGCAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCAGGCTGGGCCGGCGCTTGCGTGCTGCAACGCAGAACGACCTGGTGCGCCTGACTCGGCGCGACCTgcg                          >  5:279895/1‑136 (MQ=255)
                                                                                                                 cTGGTGGGGGTGTCGGCAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGTGCTGCAACGCAGAACGACCTGGTGCGCCTGACTCGGCGCGACCTGCGCGGGGCt                   <  4:77893/135‑1 (MQ=255)
                                                                                                                 cTGGTGGGGGTGTCGGCAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGTGCTGCAACGCAGAACGACCTGGTGCGCCTGACTCGGCGCGACCTGCGCGGGGCTg                  >  2:173879/1‑136 (MQ=255)
                                                                                                                   ggtggGGGTGTCGGCAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGTGCTGCAACGCAGAACGACCTGGTGCGCCTGACTCGGCGCGACCTGCGCGGGGCTGa                 >  2:97043/1‑135 (MQ=255)
                                                                                                                   ggtggGGGTGTCGG‑‑GCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGTGCTGCACCGCAGAACGACCTGGTGCGCCTGACTCGGCGCGACCTGCGCGGGGCTGACAg              >  2:146263/1‑136 (MQ=255)
                                                                                                                        gggTGTCGGTAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGTGCTGCAACGCAGAACGACCTGGTGCGCCt                                            <  6:374437/103‑1 (MQ=255)
                                                                                                                        gggTGTCGGTAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGTGCTGCAACGCAGAACGACCTGGTGCGCCt                                            >  5:374437/1‑103 (MQ=255)
                                                                                                                        gggTGTCGGCAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGTGCTGCAACGCAGAACGACCTGGTGCGCCTGACTCGGCGCGCCCTGCGCGGGGCTGACAGCTg           >  3:348847/1‑136 (MQ=255)
                                                                                                                        gggTGTCGGCAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGTGCTGCAACGCAGAACGACCTGGTGCGCCTGACTCGGCGCGACCTGCGCGGGGCTGACAGCTg           >  5:209102/1‑136 (MQ=255)
                                                                                                                        gggTGTCGGCAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGTGCTGCAACGCAGAACGACCTGGTGCGCCTGACTCGGCGCGACCTGCGCGGGGCTGACAGCTg           >  4:174345/1‑136 (MQ=255)
                                                                                                                        gggTGTCGG‑‑GCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGTGCTGCAACGCAGAACGACCTGGTGCGCCTGACTCGGCGCGACCTGCGCGGGGCTGACAGCTGGt         >  8:285979/1‑136 (MQ=255)
                                                                                                                            gtCGGCAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGTGCTGCAACGCAGAACGCCCTGGTGCGCCTGACTCGGCGCGACCTGCGCGGGGCTGACAGCTGGttt       >  5:251528/1‑136 (MQ=255)
                                                                                                                             tCGGCAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGTGCTGCAACGCAGAACGACCTGGTGCGCCTGACTCGGCGCGACCTGCGCGGGGCTGACAGCTGGTTTg      >  1:875/1‑136 (MQ=255)
                                                                                                                             tCGGCAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGTGCTGCAACGCAGAACGACCTGGTGCGCCTGACTCGGCGCGACCTGCGCGGGGCTGACAGCTGGTTTg      >  8:161361/1‑136 (MQ=255)
                                                                                                                                 cAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGTGCTGCAACGCAGAACGACCTGGTGCGCCTGACTCGGCGCGACCTGCGCGGGGCTGACAGCTGGTTTggcgg  >  6:314890/1‑136 (MQ=255)
                                                                                                                                  |                                                                                                                                      
CGCGTACCGGGGCTACGGCCACTTCCTATTGCCTGCACTTCATTGTGCTGGTGTCGCCGCTCAACGCCATGCAGTTCGGCGTGGCGACCATGCTGAACAGTGCCCTGGCCATGCTGGTGGGGGTGTCGGCAGCGGTCATGGCCTTTCGCTTGCTGGTGTTCCGCCATCCGGCCTGGCTGGGCCGGCGCTTGCGTGCTGCAACGCAGAACGACCTGGTGCGCCTGACTCGGCGCGACCTGCGCGGGGCTGACAGCTGGTTTGGCGG  >  ACB143/1310978‑1311242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑