Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 1,311,561 Δ1 bp 12.1% coding (1926/1989 nt) aaeB_2 → p‑hydroxybenzoic acid efflux pump subunit AaeB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1431,311,5610G.12.1% 178.5 / 19.2 58coding (1926/1989 nt)aaeB_2p‑hydroxybenzoic acid efflux pump subunit AaeB
Reads supporting (aligned to +/- strand):  ref base G (27/24);  new base . (4/3);  total (31/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.37e-01

CGTGGAGGCAGCGCTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAGCTGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGGTTTGCGTGAGTGGGAAGTGGGCGGTGTGCTGCTCAGCCCGTTTTTGCTTTATGTGTTGTTGGCGCTGCTGCTCACCGGC  >  ACB143/1311426‑1311694
                                                                                                                                       |                                                                                                                                     
cGTGGAGGCAGCGCTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAGCTGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTg                                                                                                                                       <  2:107088/136‑1 (MQ=255)
    gAGGCAGCGCTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCCGCTGCTGGATGCTGCCAGTGAGCAGGTCATTGCGGCCTTGCGTCGGCGACCCCCCCGCGCCCCGCCTCGCCGGGCCGAGGGGGCCGTACGGCAAt                                                                                                                                   >  6:245753/1‑136 (MQ=255)
     aGGCAGCGCTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAGCTGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAAtt                                                                                                                                  >  1:138717/1‑136 (MQ=255)
     aGGCAGCGCTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAGCTGCGGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGCCCCGCTTCGCCGGGCCGAGGGGGCCGTACTGGaaat                                                                                                                                  >  7:132379/1‑134 (MQ=255)
     aGGCAGCGCTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAGCGGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCATGCGTCGGCTACCCCCCACCGCCCCCCTGCGCCAGGGCGAGGGGGCAGTAAAGCaaat                                                                                                                                  >  2:193046/1‑134 (MQ=255)
             cTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAGCTGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGaaaa                                                                                                                          <  3:284614/136‑1 (MQ=255)
             cTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAGCTGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGaaaa                                                                                                                          <  3:110294/136‑1 (MQ=255)
                   aaaGGGCCTGCTGCCGGGCGTGGGCAGCTGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTggg                                                                                                                    >  8:291782/1‑136 (MQ=255)
                   aaaGGGCCTGCTGCCGGGCGTGGGCAGCAGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCGTAGCCTGGCCGTGGGGGCAGTACTGCAATTGCACAAAAGCTggg                                                                                                                    >  5:115109/1‑136 (MQ=255)
                        gCCTGCTGGCGGGCGTGGGCAGCTGCTGGATGCTGCCAGTGGGCAGTTCATTGCGGCCATGCGTCGGCTACCCGCCCGCGACCCGCTTCGACTGTCCGACGGGGCAGTACAGGCAGTCCAGCAAACCTGGGGCaa                                                                                                                >  8:78106/1‑135 (MQ=255)
                             ctgcCGGGCGTGGGCAGCTGCTGGATGCTGCCATTGAGCAGTTCATTGCGGCCTTGCGGCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTg                                                                                                          <  2:275653/136‑1 (MQ=255)
                             ctgcCGGGCGTGGGCAGCTGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTg                                                                                                          >  4:349734/1‑136 (MQ=255)
                             ctgcCGGGCGTGGGCAGCTGCTGGATGCAGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGCCCCGCGTCGCCTGGCCGAGGGGGCAGTACAGCAATTGCCGAAAAGCTGGGGCAAATGGTg                                                                                                          >  3:307032/1‑136 (MQ=255)
                                   ggCGTGGGCAGCTGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACT‑CAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTgg                                                                                                   <  6:189938/136‑1 (MQ=255)
                                       tGGGCAGCGGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACT‑CAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCagg                                                                                               <  4:360909/136‑1 (MQ=255)
                                        gggCAGCTGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCagg                                                                                               <  8:369911/136‑1 (MQ=255)
                                        gggCAGCTGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGGCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCagg                                                                                               <  8:67572/136‑1 (MQ=255)
                                         ggCAGCTGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTAGCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACT‑CAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCagga                                                                                              <  7:301064/135‑1 (MQ=255)
                                         ggCAGCTGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCagga                                                                                              <  8:93376/136‑1 (MQ=255)
                                                 ctgGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGCGGGGGCAGTACT‑CAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACAACCa                                                                                     >  2:181789/1‑136 (MQ=255)
                                                       gctgcCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGGt                                                                                >  4:368809/1‑136 (MQ=255)
                                                           ccAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGGttt                                                                              <  3:174964/134‑1 (MQ=255)
                                                            cAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGGTTTGCg                                                                           >  2:276504/1‑136 (MQ=255)
                                                             aGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGGTTTGCGt                                                                          <  1:308720/136‑1 (MQ=255)
                                                              gttaGCAGTTAATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGGTTTGCGTg                                                                         <  4:21118/133‑1 (MQ=255)
                                                              gTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGCAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGGTTTGCGTg                                                                         >  8:167677/1‑136 (MQ=255)
                                                              gTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGGTTTGCGt                                                                          >  3:242654/1‑135 (MQ=255)
                                                                   cAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGGTTTGCGTGAGTgg                                                                    <  6:81930/136‑1 (MQ=255)
                                                                          ttGCGGCCTTGCGTCGGCTACCCGCCAGCGAGCCGCTTCGCCTGGCCGAGGGGGAAGTACTGCAATTGCAGAAAAGGTGGGGCAAATGTTGCCGCTGGCAGGAGGACACCCATGGGTTTGCGTGAGTGGGAAGTgg                                                             <  4:343949/136‑1 (MQ=255)
                                                                          ttGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGGTTTGCGTGAGTGGGAAGTgg                                                             <  1:97043/136‑1 (MQ=255)
                                                                          ttGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGGTTTGCGTGAGTGGGAAGTgg                                                             <  5:83555/136‑1 (MQ=255)
                                                                          ttGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGGTTTGCGTGAGTGGGAAGTgg                                                             <  5:371524/136‑1 (MQ=255)
                                                                           tGCGGCCTTGCGTCGGCTACGCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTTCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGGTTTGCGTGAGTGGGAAGTgg                                                             <  7:131031/135‑1 (MQ=255)
                                                                              ggCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGGTTTGCGTGAGTGGGAAGTGGGCgg                                                         >  6:103726/1‑136 (MQ=255)
                                                                                   tGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGGTTTGCGTGAGTGGGAAGTGGGCGGTGtgc                                                    >  2:243212/1‑136 (MQ=255)
                                                                                   tGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGGTTTGCGTGAGTGGGAAGTGGGCGGTGtgc                                                    >  6:162759/1‑136 (MQ=255)
                                                                                   tGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGGTTTGCGTGAGTGGGAAGTGGGCGGTGtgc                                                    >  6:327236/1‑136 (MQ=255)
                                                                                   tGCGTCGGATACCCGCCAGCGCCCCGCTTCGCCTGGCAGAGGGGGCAGTACTGCAATTGCATAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGTCCACCCCTGGGTTTGAGTGAGTGGGAAGTTGGCGGTGtgc                                                    >  4:125676/1‑136 (MQ=255)
                                                                                     cGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGGTTTGCGTGAGTGGGAAGTGGGCGGTGtgct                                                   <  1:27268/135‑1 (MQ=255)
                                                                                     cGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGGTTTGCGTGAGTGGGAAGTGGGCGGTGtgct                                                   <  7:115176/135‑1 (MQ=255)
                                                                                      gTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGGTTTGCGTGAGTGGGAAGTGGGCGGTGtgctgc                                                 >  3:214312/1‑136 (MQ=255)
                                                                                      gTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGGTTTGCGTGAGTGGGAAGTGGGCGGTGtgctgc                                                 >  6:201564/1‑136 (MQ=255)
                                                                                            tACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGGTTTGCGTg                                                                         >  2:103355/1‑106 (MQ=255)
                                                                                            tACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGGTTTGCGTg                                                                         <  1:103355/106‑1 (MQ=255)
                                                                                              cccGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGGTTTGCGTGAGTGGGAAGTGGGCGGTGTGCTGCTCAGCCCg                                         <  5:295237/136‑1 (MQ=255)
                                                                                               ccGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGGTTTGCGTGAGTGGGAAGTGGGCGGTGTGCTGCTCAGCCCGt                                        <  4:222174/136‑1 (MQ=255)
                                                                                                       gACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGGTTTGCGTGAGTGGGAAGTGGGCGGTGTGCTGCTCAGCCCGTTTTTGCtt                                >  6:393579/1‑136 (MQ=255)
                                                                                                       gACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGGTTTGCGTGAGTGGGAAGTGGGCGGTGTGCTGCTCAGCCCGTTTTTGCtt                                >  4:6182/1‑136 (MQ=255)
                                                                                                       gACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGGTTTGCGTGAGTGGGAAGTGGGCGGTGTGCTGCTCAGCCCGTTTTTGCtt                                <  1:193046/136‑1 (MQ=255)
                                                                                                               tCGCCTGGCCGAGGGGGCAGTACT‑CAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGGTTTGCGTGAGTGGGAAGTGGGCGGTGTGCTGCTCAGCCCGTTGTTGCTTTATGtgttg                       >  8:148476/1‑136 (MQ=255)
                                                                                                                cGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGGTTTGCGTGAGTGGGAAGTGGGCGGTGTGCTGCTCAGCCCGTTTTTGCTTTATGtgttg                       >  3:41074/1‑136 (MQ=255)
                                                                                                                   cTGGCCGAGGGGGCAGTACTGCAATTGCAGAAACGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGGTTTGCGTGAGTGGGAAGTGGGCGGTGTGCTGCTCAGCCCGTTTTTGCTTTATGTGttgttg                    <  4:342492/136‑1 (MQ=255)
                                                                                                                   cTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGGTTTGCGTGAGTGGGAAGTGGGCGGTGTGCTGCTCAGCCCGTTTTTGCTTTATGTGttgttg                    >  4:255889/1‑136 (MQ=255)
                                                                                                                     ggCCGAGGGGGCAGTACT‑CAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGGTTTGCGTGAGTGGGAAGTGGGCGGTGTGCTGCTCAGCCCGTTTTTGCTTTATGTGTTGTTGgcg                 >  7:299121/1‑136 (MQ=255)
                                                                                                                      gCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGGTTTGCGTGAGTGGGAAGTGGGCGGTGTGCTGCTCAGCCCGTTTTTGCTTTATGTGTTGTTGgcg                 <  2:301127/136‑1 (MQ=255)
                                                                                                                         gAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGGTTTGCGTGAGTGGGAAGTGGGCGGTGTGCTGCTCAGCCCGTTTTTGCTTTATGTGTTGTTGGCgctg              >  3:283112/1‑136 (MQ=255)
                                                                                                                             gggCAGTACT‑CAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGGTTTGCGTGAGTGGGAAGTGGGCGGTGTGCTGCTCAGCCCGTTTTTGCTTTATGTGTTGTTGGCgctgctgct         >  7:278797/1‑136 (MQ=255)
                                                                                                                                    aCTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGGTTTGCGTGAGTGGGAAGTGGGCGGTGTGCTGCTCAGCCCGTTTTTGCTTTATGTGTTGTTGGCGCTGCTGCTCACCgg   >  6:228225/1‑136 (MQ=255)
                                                                                                                                     cTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGGTTTGCGTGAGTGGGAAGTGGGCGGTGTGCTGCTCAGCCCGTTTTTGCTTTATGTGTTGTTGGCGCTGCTGCTCACCGGc  <  3:144628/136‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                     
CGTGGAGGCAGCGCTGGCCAAAGGGCCTGCTGCCGGGCGTGGGCAGCTGCTGGATGCTGCCAGTGAGCAGTTCATTGCGGCCTTGCGTCGGCTACCCGCCAGCGACCCGCTTCGCCTGGCCGAGGGGGCAGTACTGCAATTGCAGAAAAGCTGGGGCAAATGGTGCCGCTGGCAGGAGGACACCCATGGGTTTGCGTGAGTGGGAAGTGGGCGGTGTGCTGCTCAGCCCGTTTTTGCTTTATGTGTTGTTGGCGCTGCTGCTCACCGGC  >  ACB143/1311426‑1311694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑