Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 5,390,745 G→A 6.6% G280D (GGC→GAC)  ligH → 10‑formyltetrahydrofolate synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,390,7450GA 6.6% 251.2 / 6.1 76G280D (GGC→GAC) ligH10‑formyltetrahydrofolate synthetase
Reads supporting (aligned to +/- strand):  ref base G (49/21);  new base A (3/2);  total (53/23)
Fisher's exact test for biased strand distribution p-value = 6.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.88e-01

TGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGACCTGGGTGCGGAGAAGTTCTTCGACATCAAATGCCGCAAGGCGGGCCTCAAGCCCTC  >  ACB143/5390613‑5390880
                                                                                                                                    |                                                                                                                                       
tGGCCAGCGAGGTGAATGCCTCGGGCGCCATGACCGTGCTGCTAAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGAGATCATCCATGGCGGCACCATCGCGAACATCGCGCATGGc                                                                                                                                        >  7:317486/1‑134 (MQ=255)
tGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTg                                                                                                                                      >  8:96084/1‑136 (MQ=255)
tGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTg                                                                                                                                      >  3:189948/1‑136 (MQ=255)
tGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTg                                                                                                                                      >  2:89001/1‑136 (MQ=255)
tGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCGGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTg                                                                                                                                      >  4:277010/1‑136 (MQ=255)
     aGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGTGAACATCGCGCATGGCTGCAACt                                                                                                                                 >  1:39071/1‑136 (MQ=255)
      gCGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGACTGCAACTc                                                                                                                                <  5:106638/136‑1 (MQ=255)
       cGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGTGCATGGCTGCAACTCg                                                                                                                               >  7:3426/1‑136 (MQ=255)
          gCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTg                                                                                                                            >  7:118954/1‑136 (MQ=255)
          gCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTg                                                                                                                            <  5:6676/136‑1 (MQ=255)
          gCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTg                                                                                                                            >  5:201918/1‑136 (MQ=255)
          gCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTg                                                                                                                            >  2:243135/1‑136 (MQ=255)
          gCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTg                                                                                                                            <  1:243135/136‑1 (MQ=255)
          gCTGAATGCCTCGGGCGCCATGACCGTGCGGCTCAAGGACGCGCTGCAGCCTAACATGGGGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTg                                                                                                                            <  6:388834/136‑1 (MQ=255)
                                aCCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCCCCAAGCCCGCGCTc                                                                                                       >  6:292283/1‑135 (MQ=255)
                                    tgctgcTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGAt                                                                                                  <  5:163888/136‑1 (MQ=255)
                                    tgctgcTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGAt                                                                                                  <  7:18852/136‑1 (MQ=255)
                                     gctgctCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATTGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGAt                                                                                                  <  3:353945/135‑1 (MQ=255)
                                       tgctCAAGTACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCCCCCAGACCGAGCTCAAGATCGc                                                                                               >  2:97072/1‑136 (MQ=255)
                                       tgctCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGc                                                                                               >  6:218553/1‑136 (MQ=255)
                                       tgctCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGc                                                                                               >  5:55640/1‑136 (MQ=255)
                                       tgctCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGc                                                                                               >  4:50035/1‑136 (MQ=255)
                                       tgctCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGc                                                                                               >  2:229551/1‑136 (MQ=255)
                                       tgctCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGc                                                                                               >  3:187263/1‑136 (MQ=255)
                                       ggctCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGc                                                                                               >  5:2660/2‑136 (MQ=255)
                                       ggctCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACACGGTGCTCGCCACCAAGACCGCGCTCAAGATCGc                                                                                               >  4:19039/2‑136 (MQ=255)
                                            aaGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGCCCGCGCTCAAGATCGCCGag                                                                                           >  6:338304/1‑134 (MQ=255)
                                            aaGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGCACGCGCTCAAGATCGCCGAtt                                                                                          >  2:405649/1‑136 (MQ=255)
                                            aaGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGAt                                                                                           >  7:127879/1‑135 (MQ=255)
                                            aaGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGAt                                                                                           >  1:234796/1‑135 (MQ=255)
                                              ggACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTAt                                                                                        <  1:211675/136‑1 (MQ=255)
                                                 cgcgCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTAtgtg                                                                                     >  6:293839/1‑136 (MQ=255)
                                                      tGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGAc                                                                                >  3:124148/1‑136 (MQ=255)
                                                      tGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGAc                                                                                >  8:74399/1‑136 (MQ=255)
                                                      tGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGAc                                                                                >  8:253178/1‑136 (MQ=255)
                                                          gCCGAACATGGTGCAGACGCGTGAGACTAATCCGGTGCTCCTCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGaa                                                                            <  6:23787/136‑1 (MQ=255)
                                                               aCATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGGGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGAAGCCgg                                                                       <  7:1283/136‑1 (MQ=255)
                                                                 aTGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCt                                                                     >  1:295001/1‑136 (MQ=255)
                                                                   ggTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTc                                                                   >  6:186986/1‑136 (MQ=255)
                                                                       cAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGt                                                                <  7:56535/135‑1 (MQ=255)
                                                                          aCGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCg                                                                  <  7:284429/130‑1 (MQ=255)
                                                                          aCGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCg                                                                  >  8:284429/1‑130 (MQ=255)
                                                                             cTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGAcc                                                         <  3:252446/136‑1 (MQ=255)
                                                                                  gaATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGACCTGGGt                                                    >  5:49244/1‑136 (MQ=255)
                                                                                  gaATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGACCTGGGt                                                    >  3:375384/1‑136 (MQ=255)
                                                                                  gaATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGACCTGGGt                                                    >  6:167531/1‑136 (MQ=255)
                                                                                  gaATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGACCTGGGt                                                    >  3:252722/1‑136 (MQ=255)
                                                                                         ccGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGACCTGGGTGCGgag                                              >  1:371086/1‑135 (MQ=255)
                                                                                           ggTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGTCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGACCTGGGTGCGGAGAAg                                           >  7:362902/1‑136 (MQ=255)
                                                                                           ggTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGACCTGGGTGCGGAGAAg                                           >  1:110366/1‑136 (MQ=255)
                                                                                           ggTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGACCTGGGTGCGGAGAAg                                           >  2:313407/1‑136 (MQ=255)
                                                                                           ggTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGACCTGGGTGCGGAGAAg                                           >  2:62430/1‑136 (MQ=255)
                                                                                           ggTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGACCTGGGTGCGGAGAAg                                           >  1:60099/1‑136 (MQ=255)
                                                                                             tGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGACCTGGGTGCGGAGAAGtt                                         <  4:67015/136‑1 (MQ=255)
                                                                                             tGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGACCTGGGTGCGGAGAAGtt                                         <  1:89001/136‑1 (MQ=255)
                                                                                               cTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGACCTGGGTGCGGAGAAGttct                                       <  3:124203/136‑1 (MQ=255)
                                                                                               cTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGACCTGGGTGCGGAGAAGttct                                       <  3:352630/136‑1 (MQ=255)
                                                                                                tCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGACGCCGGCTTCGGTGCCGCCCTGGGTGCGGCGAAGtgctt                                      >  7:97670/1‑136 (MQ=255)
                                                                                                tCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGACCTGGGTGCGGAGAAGttct                                       >  3:16498/1‑135 (MQ=255)
                                                                                                tCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGACTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGACCTGGGTGCGGAGAAGttctt                                      >  8:12568/1‑136 (MQ=255)
                                                                                                     cATGGCGGCCCGTTCGCGAACATCGCGCATGACTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGCCCTGGGTGCGGAGAAGTTCTTCGACa                                 >  7:264930/1‑136 (MQ=255)
                                                                                                          cggcCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGACCTGGGTGCGGAGAAGTTCTTCGACATCaaa                            >  7:19350/1‑136 (MQ=255)
                                                                                                          cggcCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGACCTGGGTGCGGAGAAGTTCTTCGACATCaaa                            >  7:9038/1‑136 (MQ=255)
                                                                                                          cggcCCGTTCGCGAACATCGCGCATGACTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGACCTGGGTGCGGAGAAGTTCTTCGACATCaaa                            >  7:124576/1‑136 (MQ=255)
                                                                                                               cGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGCTCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGACCTGGGTGCGGAGAAGTTCTTCGACATCAAATGCCg                       >  2:9117/1‑136 (MQ=255)
                                                                                                               cGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGCCCTGGGTGCGGAGAAGTTCTTCGACATCAAATGCCg                       >  1:109054/1‑136 (MQ=255)
                                                                                                               cGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGCCCTGGGTGCGGAGAAGTTCTTCGACATCAAATGCCg                       >  6:206518/1‑136 (MQ=255)
                                                                                                                 ttCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGACCTGGGTGCGGAGAAGTTCTTCGACATCAAATGCCGCa                     <  8:118954/136‑1 (MQ=255)
                                                                                                                    gcgAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGACCTGGGTGCGGAGAAGTTCTTCGACATCAAATGCCGCAAg                   <  2:322664/135‑1 (MQ=255)
                                                                                                                    gcgAACATCGCGCATGACTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGACCTGGGTGCGGAGAAGTTCTTCGACATCAAATGCCGCAAg                   <  6:67215/135‑1 (MQ=255)
                                                                                                                    gcgAACATCGCGCAGGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGAGGGAAGCCGGCTTCGGTGCCGACCTGGGTGCGGAGAAGTTCTTCGACATCAAATGCCGCAAg                   <  2:188458/135‑1 (MQ=255)
                                                                                                                               gcATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGAAGCCgg                                                                       <  3:309954/72‑1 (MQ=255)
                                                                                                                               gcATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGAAGCCgg                                                                       >  4:309954/1‑72 (MQ=255)
                                                                                                                               gcATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGACCTGGGTGCGGAGAAGTTCTTCGACATCAAATGCCGCAAGGCGGGCCTCAAg       >  1:254449/1‑136 (MQ=255)
                                                                                                                                cATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGACCTGGGTGCGGAGAAGTTCTTCGACATCAAATGCCGCAAGGCGGGCCTCAAGc      <  2:377635/136‑1 (MQ=255)
                                                                                                                                 aTGGCTGCAACTCGGTGCTCGCAACCAAGAACGCGCTAAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGACCTGGGTGCGGAGAAGTTCTTCGACATCAAATGCCGCAAGGCGGGCCTCAAGca     >  8:72880/1‑135 (MQ=255)
                                                                                                                                    gCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGACCTGGGTGCGGAGAAGTTCTTCGACATCAAATGCCGCAAGGCGGGCCTCAAGCCCTc  <  2:234796/136‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                       
TGGCCAGCGAGCTGAATGCCTCGGGCGCCATGACCGTGCTGCTCAAGGACGCGCTGCAGCCGAACATGGTGCAGACGCTGGAGAATAATCCGGTGCTCATCCATGGCGGCCCGTTCGCGAACATCGCGCATGGCTGCAACTCGGTGCTCGCCACCAAGACCGCGCTCAAGATCGCCGATTATGTGGTGACGGAAGCCGGCTTCGGTGCCGACCTGGGTGCGGAGAAGTTCTTCGACATCAAATGCCGCAAGGCGGGCCTCAAGCCCTC  >  ACB143/5390613‑5390880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑