Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 5,861,107 G→A 5.6% R746R (CGC→CGT)  cadA_2 ← Cadmium‑transporting ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1435,861,1070GA 5.6% 237.4 / 3.2 71R746R (CGC→CGT) cadA_2Cadmium‑transporting ATPase
Reads supporting (aligned to +/- strand):  ref base G (38/29);  new base A (2/2);  total (40/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.43e-01

CGGATCTGCGTGGTCACCGTGGGCTGGCTGAGGCCAAGCTTCTTCGCCGCCTGGGTGATGCTGCCCAGACGGGCCACCATGTAGAAGGCCTTCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCATCCACATCGTGGCCATGCCGGCGAAGGTGATCGCCAGAAATATCGCCTTGATGCCCAACGCCAGCACGATGTTCTGCAT  >  ACB143/5860973‑5861241
                                                                                                                                      |                                                                                                                                      
cGGATCTGCGTGGTCACCGTGGGCTGGCTGAGGCCAAGCTTCTTCGCCGCCTGGGTGATGCTGCCCAGACGGGCCACCATGTAGAAGGCCTTCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGgc                                                                                                                                       >  4:243210/1‑136 (MQ=255)
   aTCTGCGTGGTCACCGTGGGCTGGCTGAGGCCAAGCTTCTTCGCCGCCTGGGTGATGCTGCCCAGACGGGCCACCATGTAGAAGGCCTTCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGgcgc                                                                                                                                     >  1:281056/1‑135 (MQ=255)
   aTCTGCGTGGTCACCGTGGGCTGGCTGAGGCCAAGCTTCTTCGCCGCCTGGGTGATGCTGCCCAGACGGGCCACCATGTAGAAGGCCTTCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGgcgc                                                                                                                                     >  7:249382/1‑135 (MQ=255)
   aTCTGCGTGGTCACCGTGGGCTGGCTGAGGCCAAGCTTCTTCGCCGCCTGGGTGATGCTGCCCAGACGGGCCACCATGTAGAAGGCCTTCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGgcgc                                                                                                                                     >  1:381699/1‑135 (MQ=255)
        cGTGGTCACCGTGGGCTGGCTGAGGCCAAGCTTCTTCGCCGCCTGGGTGATGCTGCCCAGACGGGCCACCATGTAGAAGGCCTTCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGc                                                                                                                                 <  2:130757/134‑1 (MQ=255)
        cGTGGTCACCGTGGGCTGGCTGAGGCCAAGCTTCTTCGCCGCCTGGGTGATGCTGCCCAGACGGGCCACCATGTAGAAGGCCTTCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCg                                                                                                                               <  7:189083/136‑1 (MQ=255)
          tGGTCACCGTGGGCTGGCTGAGGCCAAGCTTCTTCGCCGCCTGGGTGATGCTGCCCAGACGGGCCACCATGTAGAAGGCCTTCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGtt                                                                                                                             <  7:212876/136‑1 (MQ=255)
           ggTCACCGTGGGCTGGCTGAGGCCAAGCTTCTTCGCCGCCTGGGTGATGCTGCCCAGACGGGCCACCATGTAGAAGGCCTTCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGtt                                                                                                                             >  6:257567/1‑135 (MQ=255)
                ccGTGGGCTGGCTGAGGCCAAGCTTCTTCGCCGCCTGGGTGATGCTGCCCAGACGGGCCACCATGTAGAAGGCCTTCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGgc                                                                                                                                       <  1:216294/120‑1 (MQ=255)
                ccGTGGGCTGGCTGAGGCCAAGCTTCTTCGCCGCCTGGGTGATGCTGCCCAGACGGGCCACCATGTAGAAGGCCTTCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGgc                                                                                                                                       >  2:216294/1‑120 (MQ=255)
                ccGTGGGCTGGCTGAGGCCAAGCTTCTTCGCCGCCTGGGTGATGCTGCCCAGACGGGCCACCATGTAGAAGGCCTTCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAacac                                                                                                                       >  3:360027/1‑136 (MQ=255)
                   tGGGCTGGCTGAGGCCAAGCTTCTTCGCCGCCTGGGTGATGCTGCCCAGACGGGCCACCATGAAGAAGGCCTTCAGCTCGGTACTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGATGCCcacccc                                                                                                                    >  8:140489/1‑134 (MQ=255)
                    gggCTGGCTGAGGCCAAGCTTCTTCGCCGCCTGGGTGATGCTGCCCAGACGGGCCACCATGTAGAAGGCCTTCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACaccacc                                                                                                                   <  6:51728/136‑1 (MQ=255)
                          gctgAGGCCAAGCTTCTTCGCCGCCTGGGTGATGCTGCCCAGACGGGCCACCATGTAGAAGGCCTTCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCaa                                                                                                              <  6:362003/135‑1 (MQ=255)
                          gctgAGGCCAAGCTTCTTCGCCGCCTGGGTGATGCTGCCCAGACGGGCCACCATGTAGAAGGCCTTCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAg                                                                                                             >  6:345153/1‑136 (MQ=255)
                             gAGGCCAAGCTTCTTCGCCGCCTGGGTGATGCTGCCCAGACGGGCCACCATGTAGAAGGCCTTCAGCTCGGAACTCCGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCt                                                                                                           >  8:103765/1‑135 (MQ=255)
                              aGGCCAAGCTTCTTCGCCGCCTGGGTGATGCTGCCCAGACGGGCCACCATGTAGAAGGCCTTCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCt                                                                                                           <  3:348750/134‑1 (MQ=255)
                               ggCCAAGCTGCTTCGCCGCCTGGGTGATGCTGCCCATAGGGGCCACCATGTAGAAGGCCTTCAGCTCGGAACTCAGCATTTACGTCCTCTATTTGCGCAAAAGGCGAAAGCCGTTGAACACCACCAGCAAGCTCAc                                                                                                        <  6:230189/136‑1 (MQ=255)
                                 ccAAGCTTCTTCGCCGCCTGGGTGATGCTGCCCAGACGGGCCACCATGTAGAAGGCCTTCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGc                                                                                                      <  6:68653/136‑1 (MQ=255)
                                   aaGCTTCTTCGCCGCCTGGGTGATGCTGCCCAGACGGGCCACCATGTAGAAGGCCTTCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGcc                                                                                                     >  1:272756/1‑135 (MQ=255)
                                       ttcttcGCCGCCTGGGTGATGCTGCCCAGACGGGCCACCATGTAGAAGGCCTTCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCatat                                                                                                >  3:81757/1‑136 (MQ=255)
                                       ttcttcGCCGCCTGGGTGATGCTGCCCAGACGGGCCACCATGTAGAAGGCCTTCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCatat                                                                                                >  4:312929/1‑136 (MQ=255)
                                       ttcttcGCCGCCTGGGTGATGCTGCCCAGACGGGCCACCATGTAGAAGGCCTTCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCata                                                                                                 >  1:281607/1‑135 (MQ=255)
                                       ttcttcGCCGCCTGGGTGATGCTGCCCAGACGGGCCACCATGTAGAAGGCCTTCAGATCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCatat                                                                                                >  3:223576/1‑136 (MQ=255)
                                            cgccgcCTGGGTGATGCTGCCCAGACGGGCCACCATGTAGAAGGCCTTCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGc                                                                                            <  7:259742/135‑1 (MQ=255)
                                             gccgccTGGGTGATGCTGCCCAGACGGGCCACCATGTAGAAGGCCTTCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCg                                                                                           >  8:239943/1‑135 (MQ=255)
                                             gccgccTGGGTGATGCTGCCCAGACGGGCCACCATGTAGAAGGCCTTCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGa                                                                                          <  5:248224/136‑1 (MQ=255)
                                              ccgccTGGGTGATGCTGCCCAGACGGGCCACCATGTAGAAGGCCTTCAGCTCGGAACTCAGGATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGaa                                                                                         <  5:191350/136‑1 (MQ=255)
                                                gccTGGGTGATGCTGCCCAGACGGGCCACCATGTAGAAGGCCTTCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAaca                                                                                       <  4:392838/136‑1 (MQ=255)
                                                gccTGGGTGATGCTGCCCAGACGGGCCACCATGTAGAAGGCCTTCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAaca                                                                                       <  4:392836/136‑1 (MQ=255)
                                                 ccTGGGTGATGCTGCCCAGACGGGCCACCATGTAGAAGGCCTTCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAacac                                                                                      <  5:371559/136‑1 (MQ=255)
                                                      gTGATGCTGCCCAGACGGGCCACCATGTAGAAGGCCTTCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCa                                                                                 <  6:340460/136‑1 (MQ=255)
                                                                  aGACGGGCCACCATGTAGAAGGCCTTCAGCTCGGTACTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCTTCCACATCGTgg                                                                     >  3:43043/1‑136 (MQ=255)
                                                                     cGGGCCACCATGTAGAAGGCCTTCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCATCCACATCGTGGCCa                                                                  <  1:136640/136‑1 (MQ=255)
                                                                            ccaTGTAGAAGGCCTTCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCCTATCAGCGAACACCGCCATCCACATCGTGGCCATGCCGGc                                                           >  2:254847/1‑136 (MQ=255)
                                                                            ccaTGTAGAAGGCCTTCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCATCCACATCGTGGCCATGCCGGc                                                           >  3:280578/1‑136 (MQ=255)
                                                                                   gAAGGCCTTCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCATCCACATCGTGGCCATGCCGGCGAAGGt                                                     <  1:247683/135‑1 (MQ=255)
                                                                                     aGGCCTTCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCATCCACATCGTGGCCATGCCGGCGAAGGTGAt                                                  >  3:187213/1‑136 (MQ=255)
                                                                                     aGGCCTTCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGAGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCATCCACATCGTGGCCATGCCGGCGAAGGTGAt                                                  >  1:12573/1‑136 (MQ=255)
                                                                                        ccTTCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCATCCACATCGTGGCCATGCCGGCGAAGGTGATCGc                                               >  1:49028/1‑136 (MQ=255)
                                                                                         cTTCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCATCCACATCGTGGCCATGCCGGCGAAGGTGATCGc                                               >  3:107954/1‑135 (MQ=255)
                                                                                         cTTCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCATCCACATCGTGGCCATGCCGGCGAAGGTGATCGc                                               >  5:245190/1‑135 (MQ=255)
                                                                                          ttCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCACGCTCACGCCCATCTCAGCGAACACCGCCGTCCACATCGTGGCCAGGCCGGCGAAGGTGATCGc                                               >  2:298763/1‑134 (MQ=255)
                                                                                          ttCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCATCCACATCGTGGCCATGCCGGCGAAGGTGATCGc                                               >  6:108716/1‑134 (MQ=255)
                                                                                          ttCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCATCCACATCGTGGCCATGCCGGCGAAGGTGATCGc                                               >  8:363032/1‑134 (MQ=255)
                                                                                          ttCAGCTAGGAACTCAGCATGTACGTACTCTATTTGCGCAAAAGGCGCAAGAAGTTGAAAACCACCAGCCAGCTCACGCCAATATAAGCGAACACCGCCATCCAAATCGTGGCCATGCCGGCGAATATGATCGCCa                                             >  2:85436/1‑136 (MQ=255)
                                                                                          atCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCATCCACATCGTGGCCATCCCGGCGAAGGTGATCGc                                               >  2:51486/2‑134 (MQ=255)
                                                                                              gCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCATCCACATCGTGGCCATGCCGGCGAAGGTGATCGCCAGaaa                                         >  1:371488/1‑136 (MQ=255)
                                                                                                     aCTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCATCCACATCGTGGCCATGCCGGCGAAGGTGATCGCCAGAAATATCGcc                                  <  4:228632/136‑1 (MQ=255)
                                                                                                     aCTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCATCCACATCGTGGCCATGCCGGCGAAGGTGATCGCCAGAAATATCGcc                                  <  8:352729/136‑1 (MQ=255)
                                                                                                      cTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCATCCACATCGTGGCCATGCCGGCGAAGGTGATCGCCAGAAATATCGCCt                                 <  5:178752/136‑1 (MQ=255)
                                                                                                           cATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCATCCACATCGTGGCCATGCCGGCGAAGGTGATCGCCAGAAATATCGCCTTGATg                            <  4:108157/136‑1 (MQ=255)
                                                                                                            aTGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCATCCACATCGTGGCCATGCCGGCGAAGGTGATCGCCAGAAATATCGCCTTGATGc                           >  6:160023/1‑136 (MQ=255)
                                                                                                            aTGTACGTCCTCTATTTGCGCAAAAGACGCAAGCCGTTGAACACCACCAGCaa                                                                                                              <  8:65683/53‑1 (MQ=255)
                                                                                                            aTGTACGTCCTCTATTTGCGCAAAAGACGCAAGCCGTTGAACACCACCAGCaa                                                                                                              <  8:71113/53‑1 (MQ=255)
                                                                                                            aTGTACGTCCTCTATTTGCGCAAAAGACGCAAGCCGTTGAACACCACCAGCaa                                                                                                              >  7:65683/1‑53 (MQ=255)
                                                                                                            aTGTACGTCCTCTATTTGCGCAAAAGACGCAAGCCGTTGAACACCACCAGCaa                                                                                                              >  7:71113/1‑53 (MQ=255)
                                                                                                                 cGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCATCCACATCGTGGCCATGCCGGCGAAGGTGATCGCCAGAAATATCGCCTTGATGCCCAAc                      >  1:349498/1‑136 (MQ=255)
                                                                                                                 cGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCATCCACATCGTGGCCATGCCGGCGAAGGTGATCGCCAGAAATATCGCCTTGATGCCCAAc                      >  8:282916/1‑136 (MQ=255)
                                                                                                                 cGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCATCCACATCGTGGCCATGCCGGCGAAGGTGATCGCCAGAAATATCGCCTTGATGCCCAAc                      >  5:120095/1‑136 (MQ=255)
                                                                                                                  gTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCATCCACATCGTGGCCATGCCGGCGAAGGTGATCGCCAGAAATATCGCCTTGATGCCCAACg                     <  4:329550/136‑1 (MQ=255)
                                                                                                                    ccTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCATCCACATCGTGGCCATGCCGGCGAAGGTGATCGCCAGAAATATCGCCTTGATGCCCAACg                     <  6:323507/134‑1 (MQ=255)
                                                                                                                     ctctATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCATCCACATCGTGGCCATGCCGGCGAAGGTGATCGCCAGAAATATCGCCTTGATGCCCAACGCCa                  <  3:176890/136‑1 (MQ=255)
                                                                                                                     ctctATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCATCCACATCGTGGCCATGCCGGCGAAGGTGATCGCCAGAAATATCGCCTTGATGCCCAACGCCa                  <  8:249382/136‑1 (MQ=255)
                                                                                                                     ctctATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCATCCACATCGTGGCCATGCCGGCGAAGGTGATCGCCAGAAATATCGCCTTGATGCCCAACGCCa                  <  2:276085/136‑1 (MQ=255)
                                                                                                                      tctATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCATCCACATCGTGGCCATGCCGGCGAAGGTGATCGCCAGAAATATCGCCTTGATGCCCAACGCCAg                 >  1:79626/1‑136 (MQ=255)
                                                                                                                        tATTTGCGCAAAAGGCGCAAGCCGTTGAACACCCCCAGCAAGCTCCCGCCCATATCAGCGAACACCGCCATCCACATCGTGGCCATGCCGGCGAAGGTGATCGCCAGAAATATCGCCTTGATGCCCAACGCCAGCa               >  6:331283/1‑136 (MQ=255)
                                                                                                                        tATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCATCCACATCGTGGCCATGCCGGCGAAGGTGATCGCCAGAAATATCGCCTTGATGCCCAACGCCAGCa               >  2:120981/1‑136 (MQ=255)
                                                                                                                        tATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCATCCACATCGTGGCCATGCCGGCGAAGGTGATCGCCAGAAATATCGCCTTGATGCCAAACGCCAGCa               >  8:238625/1‑136 (MQ=255)
                                                                                                                          tttGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCATCCACATCGTGGCCATGCCGGCGAAGGTGATCGCCAGAAATATCGCCTTGATGCCCAACGCCAGc                <  7:332455/133‑1 (MQ=255)
                                                                                                                            tGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCATCCACATCGTGGCCATGCCGGCGAAGGTGATCGCCAGAAATATCGCCTTGATGCCCAACGCCAGCACGAt           >  5:105662/1‑136 (MQ=255)
                                                                                                                             gctcAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCATCCACATCGTGGCCATGCCGGCGAAGGTGATCGCCAGAAATATCGCCTTGATGCCCAACGCCAGCACGAt           <  5:44880/132‑1 (MQ=255)
                                                                                                                             gcgcAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCATCCACATCGTGGCCATGCCGGCGAAGGTGATCGCCAGAAATATCGCCTTGATGCCCAACGCCAGCACGAt           <  7:123820/135‑1 (MQ=255)
                                                                                                                             gcgcAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCATCCACATCGTGGCCATGCCGGCGAAGGTGATCGCCAGAAATATCGCCTTGATGCACAACGCCAGCACGATg          >  5:289683/1‑136 (MQ=255)
                                                                                                                                 aaaaGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCATCCACATCGTGGCCATGCCGGCGAAGGTGATCGCCAGAAATATCGCCTTGATGCCCAACGCCAGCACGATGTTCt      >  4:264477/1‑136 (MQ=255)
                                                                                                                                  aaaGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCATCCACATCGTGGCCATGCCGGCGAAGGTGATCGCCAGAAATATCGCCTTGATGCCCAACGCCAGCACGATGTTCTg     >  4:211066/1‑136 (MQ=255)
                                                                                                                                   aaGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCATCCACATCGTGGCCATGCCGGCGAAGGTGATCGCCAGAAATATCGCCTTGATGCCCAACGCCAGCACGATGTTCTg     >  6:296286/1‑135 (MQ=255)
                                                                                                                                     ggCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCATCCACATCGTGGCCATGCCGGCGAAGGTGATCGCCAGAAATATCGCCTTGATGCCCAACGCCAGCACGATGTTCTGCAt  >  3:385054/1‑136 (MQ=255)
                                                                                                                                      |                                                                                                                                      
CGGATCTGCGTGGTCACCGTGGGCTGGCTGAGGCCAAGCTTCTTCGCCGCCTGGGTGATGCTGCCCAGACGGGCCACCATGTAGAAGGCCTTCAGCTCGGAACTCAGCATGTACGTCCTCTATTTGCGCAAAAGGCGCAAGCCGTTGAACACCACCAGCAAGCTCACGCCCATATCAGCGAACACCGCCATCCACATCGTGGCCATGCCGGCGAAGGTGATCGCCAGAAATATCGCCTTGATGCCCAACGCCAGCACGATGTTCTGCAT  >  ACB143/5860973‑5861241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑