Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	ACB143	211,447	G→A	6.2%	G8769D (GGC→GAC)	CJ019_00185 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	ACB143	211,447	0	G	A	6.2%	217.5 / 3.4	65	G8769D (GGC→GAC)	CJ019_00185	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (36/24);  new base A (2/2);  total (38/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.35e-01

TCACGGCCACCTGGGGAGCCGGTGGCGGGGTGTCGGGTACCTTTACCCCAAATACCAGCGGCTACTACCCGATCGAGGTCTACCATGCCAACCAGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACGTCAAGATGTACCAGAACGTTACCGAGATGGCGAACGCAGGCCTGGGCGTGTCCGACCTGCACACCGTGAATGGTCAGAGCTACTACGACGGCTACAAGCT  >  ACB143/211315‑211582                                                                                                                                     |                                                                                                                                        tCACGGCCACCTGGGGAGCCGGTGGCGGGGTGTCGGGTACCTTTACCCCAAATACCAGCGGCTACTACCCGATCGAGGTCTACCATGCCAACCAGTCCGGTCCAGGCAGCTATGACCTGAACCTCCAGGTAGGCTc                                                                                                                                      >  3:241408/1‑136 (MQ=255)    cGGCCACCTGGGGAGCCGGTGGCGGGGTGTCGGGTACCTTTACCCCAAATACCAGCGGCTACTACCCGATCGAGGTCTACCATGCCAACCAGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCgg                                                                                                                                   <  6:315018/136‑1 (MQ=255)      ggcACCTGGGGAGCCGGTGGCGGGGTGTCGGGTACCTTAACCCCAAAGACCAGCGGCTAATACCCCATCGATGTGTACCCTGCCAACCAGTCCGGTCCAGGCAGCTATGACCTGCACATCCCGGTAGGCTCCCGTg                                                                                                                                 >  8:169013/3‑136 (MQ=255)      gCCACCTGGGGAGCCGGTGGCGGGGTGTCGGGTACCTTTACCCCAAATACCAGCGGCTACTACCCGATCGAGGTCTACCATGCCAACCAGTCCGGTCCAGGCAGCGATGACCTGAACATCCAGGTAGGCTCCGGTg                                                                                                                                 >  7:138564/1‑136 (MQ=255)      gCCACCTGGGGAGCCGGTGGCGGGGTGTCGGGTACCGTTACCCCAAATACCAGCGGCTACTACCCGATCGAGGGCTACCATGCCAACCAGTCCGGTCCAGGCAGCGATGACCTGAACATCCAGGTAGGCTACgggt                                                                                                                                 >  6:204200/1‑134 (MQ=255)      gCCACCTGGGGAGCAGGTGGCGGGGTGTCGGGTACCTTTACCCCAAATACCAGCGGCTACTACCCGATCGAGGTCTACCATGCCAACCAGTCCGGTCCAGGCAGCTATGACCTGAACATACAGGTAGACTCCGGTg                                                                                                                                 >  7:207715/1‑136 (MQ=255)       ccACCTGGGGAGCCGGTGGCGGGGTGTCGGGTACCTTTACCCCAAATACCAGCGGCTACTACCCGATCGAGGTCTACCATGCCAACCAGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGTGc                                                                                                                                >  3:149068/1‑136 (MQ=255)         aCCTGGGGAGCCGGTGGCGGGGTGTCGGGTACCTTTACCCCAAATACCAGCGGCTACTACCCGATCGAGGTCTACCATGCCAACCAGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGTGCCg                                                                                                                              <  1:208447/136‑1 (MQ=255)         aCCTGGGGAGCCGGTGGCGGGGTGTCGGGTACCTTTACCCCAAATACCAGCGGCTACTACCCGATCGAGGTCTACCATGCCAACCAGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGTGCCg                                                                                                                              <  4:185687/136‑1 (MQ=255)         aCCTGGGGAGCCGGTGGCGGGGTGTCGGGTACCTTTACCCCAAATACCAGCGGCTACTACCCGATCGAGGTCTACCATGCCAACCAGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGGGCCg                                                                                                                              <  3:138383/136‑1 (MQ=255)                  gCCGGTGGCGGGGTGTCGGGTACCTTTACCCCAAATACCAGCGGCTACTACCCGATCGAGGTCTACCATGCCAACCAGTCCGGTGCAGGCAGCTATGACCTGAACATCCAGGTAGGCTGCGGTGCAGTTACCGccc                                                                                                                     >  6:206185/1‑136 (MQ=255)                    cGGTGGCGGGGTGTCGGGTACCTTTTCCCCAAATACCAGCGGCTACTACCCGATCGAGGTCTACCATTCCCACCAGTCCCGTCCAGGCCGCTATGACCGGAACATACAGGTAGGCAGCGGTGCCGTTAACGACCt                                                                                                                    >  2:398383/1‑135 (MQ=255)                    cGGTGGCGGGGTGTCGGGTACCTTTACCCCAAATACCAGCGGCTACTACCCGATCGAGGTCTACCATGCCAACCAGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGTGCCGTTACCGACCt                                                                                                                    >  7:371470/1‑135 (MQ=255)                    cGGTGGCGGGGTGTCGGGTACCTTTACCCCAAATACCAGCGGCTAATACCCGATCGAGGTCTACCATGCCAACCAGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGTGCCGTTACCGACCt                                                                                                                    >  1:126772/1‑135 (MQ=255)                      gTGGCGGGGTGTCGGGTACCTTTACCCCAAATACCAGCGGCTACTACCCGATCGAGGTCTACCATGCCAACCAGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGTGCCGTTACCGACCTGag                                                                                                                 >  2:271190/1‑136 (MQ=255)                        tccGGGGTGTCGGGTACCTTTACCCCAAATACCAGAGGCTACTAACCGATCGAGGTCTACCATGCCAACCAGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGTGCCGTTACCGACCTGag                                                                                                                 >  8:45876/3‑134 (MQ=255)                        ggCGGGGTGTCGGGTACCTTTACCCCAAATACCAGCGGCTACTACCCGATCGAGGTCTACCATGCCAACCAGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGTGCCGTTACCGACCTGag                                                                                                                 >  5:315111/1‑134 (MQ=255)                          cGGGGTGTCGGGTACCTTTACCCCAAATACCAGCGGCTACTACCCGATCGAGGTCTACCATGCCAACCAGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGTGCCGTTACCGACCTGagcagc                                                                                                             <  1:313847/136‑1 (MQ=255)                                 tCGGGTACCTTTACCCCAAATACCAGCGGCTACTACCCGATCGAGGTCTACCATGCCAACCAGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACg                                                                                                      <  6:7916/136‑1 (MQ=255)                                     gTACCTTTACCCCAAATACCAGCGGCTACTACCCGATCGAGGTCTACCATGCCAACCAGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACGTCaa                                                                                                  <  5:33950/136‑1 (MQ=255)                                            tACCCCAAATACCAGCGGCTACTACCGGATCGAGGTCTACCATGCCAACCAGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACGTCAAGATGTa                                                                                            <  6:61460/135‑1 (MQ=255)                                            tACCCCAAATACCAGCGGCTACTACCCGATCGAGGTCTACCATGCCAACCAGTCCGGTCCAGGCAGCTATGACCTGAACATCGAGGTAGGCTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACGTGAAGATGTa                                                                                            <  6:36288/135‑1 (MQ=255)                                            tACCCCAAATACCAGCGGCTACTACCCGATCGAGGTCTACCATGCCAACCAGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACGTCAAGATGTa                                                                                            <  1:343104/135‑1 (MQ=255)                                            tACCCCAAATACCAGCGGCTACTACCCGATCGAGGTCTACCATGCCAACCAGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACGTCAAGATGTAc                                                                                           >  8:18791/1‑136 (MQ=255)                                              ccccAAATACCAGCGGCTACTACCCGATCGAGGTCTACCATGCCAACCAGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGACTCCGGTGCCGTTAcc                                                                                                                         >  1:247716/1‑104 (MQ=255)                                              ccccAAATACCAGCGGCTACTACCCGATCGAGGTCTACCATGCCAACCAGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGACTCCGGTGCCGTTAcc                                                                                                                         <  2:247716/104‑1 (MQ=255)                                                     tACCAGCGGCTACTACCCGATCGAGGTCTACCATGCCAACCAGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACGTCAAGATGTACCAGAACGt                                                                                   >  4:328363/1‑135 (MQ=255)                                                        cAGCGGCTACTACCCGATCGAGGTCTACCATGCCAACCATTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACGTCAAGATGTACCAGAACGTTAcc                                                                               <  7:86825/136‑1 (MQ=255)                                                        cAGCGGCTACTACCCGATCGAGGTCTACCATGCCAACCAGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACGTCAAGATGTACCAGAACGTTAcc                                                                               <  3:143991/136‑1 (MQ=255)                                                        cAGCGGCTACTACCCGATCGAGGTCTACCATGCCAACCAGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGAGCCGTTACCGACCTGAGCAGCTCGAACGTCAAGATGTACCAGAACGTTAcc                                                                               <  1:154675/136‑1 (MQ=255)                                                                actacCCGATCGAGGTCTACCATGCCAACCAGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACGTCAAGATGTACCAGAACGTTACCGAGATGGc                                                                       >  2:260309/1‑136 (MQ=255)                                                                actacCCGATAGAGGTCTACAATGCCAAACAGTCCGGTCCAGGCAGCTATGACCTGAAAATCCAGGTAGGCTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACGTCAAGATGTACCAGAACGATACCGAGATGGc                                                                       >  2:255262/1‑136 (MQ=255)                                                                       gATCGAGGTCTACCATGCCAACCAGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGATGCCGTTACCGACCTGAGCAGCTCGAACGTCAAGATGTACCAGAACGTTACCGAGATGGCGAACGCa                                                                >  5:246287/1‑136 (MQ=255)                                                                           gAGGTCTACCATGCCAACCAGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACg                                                                                                      >  2:7714/1‑94 (MQ=255)                                                                           gAGGTCTACCATGCCAACCAGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACg                                                                                                      <  1:7714/94‑1 (MQ=255)                                                                           gAGGTCTACCATGCCAACCAGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACGTCAAGATGTACCAGAACGTTACCGAGATGGCGAACGCAGGcc                                                            >  4:78005/1‑136 (MQ=255)                                                                           gAGGTCTACCATGCCAACCAGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACGTCAAGATGTACCAGAACGTTACCGAGATGGCGAACGCAGGcc                                                            >  4:151429/1‑136 (MQ=255)                                                                           gAGGTCTACCATGCCAACCAGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACGTCAAGATGTACCAGAACGTTACCGAGATCGCGAACGCAGGcc                                                            >  2:25173/1‑136 (MQ=255)                                                                            aGGTCTACCATGCCAACCAGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACGTCAAGATGTACCAGAACGTTACCGAGATGGCGAACGCAGGCCt                                                           <  1:5533/136‑1 (MQ=255)                                                                                 tACCATGCCAACCAGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACGTCAAGATGTACCAGAACGTTACCGAGATGGCGAACGCAGGCCTGGGCg                                                      >  7:157877/1‑136 (MQ=255)                                                                                        ccAACCAGTCCGGTCCAGGCAGCTAAGACCTGAACATCCAGGTAGGCTCCGGTTCCGTTACCGACCTGAGCAGCTCGAACGTCAAGATGTACCAGAACGTTACCTAGATGGCGAACGCAGGCCTGGGCGTGTCCGa                                               <  7:169013/136‑1 (MQ=255)                                                                                         cAACCAGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACGTCAAGATGTACCAGAACGTTACCGAGATGGCGAACGCAGGCCTGGGCGTGTCCGAc                                              <  2:171313/136‑1 (MQ=255)                                                                                         cAACCAGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACGTCAAGATGTACCAGAACGTTACCGAGATGGCGAACGCAGGCCTGGGCGTGTCCGAc                                              >  8:109325/1‑136 (MQ=255)                                                                                          aaCCAGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACGTCAAGATGTACCAGAACGTTACCGAGATGGCGAACGCAGGCCTGGGCGTGTCCGAcc                                             >  5:159194/1‑136 (MQ=255)                                                                                           aCCAGTCCGGTCCAGGCAGCTATGTCCTGAACATCCAGGTAGGGTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACGTCAAGATGTACCAGAACGTTACCGAGATGGCGAACGCAGGCCTGGGCGTGTCCGACCt                                            <  7:224001/136‑1 (MQ=255)                                                                                           aCCAGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACGTCAAGATGTACCAGAACGTTACCGAGATGGCGAACGCAGGCCTGGGCGTGTCCGACCt                                            >  8:62057/1‑136 (MQ=255)                                                                                           aCCAGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACGTCAAGATGTACCAGAACGTTACCGAGATGGCGAACGCAGGCCTGGGCGTGTCCGACCt                                            <  4:208013/136‑1 (MQ=255)                                                                                            ccAGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACGTCa                                                                                                   >  6:346671/1‑80 (MQ=255)                                                                                            ccAGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACGTCa                                                                                                   <  5:346671/80‑1 (MQ=255)                                                                                              aGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACGTCAAGATGTACCAGAACGTTACCGAGATGGCGAACGCAGGCCTGGGCg                                                      <  4:152373/123‑1 (MQ=255)                                                                                              aGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACGTCAAGATGTACCAGAACGTTACCGAGATGGCGAACGCAGGCCTGGGCg                                                      >  3:152373/1‑123 (MQ=255)                                                                                              aGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGACTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACGTCAAGATGTACCAGAACGTTACCGAGATGGCGAACGCAGGCCTGGGCGTGTCCGACCTGca                                         <  8:207715/136‑1 (MQ=255)                                                                                                tCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACGTCAAGATGTACCAGAACGTTACCGAGATGGCGAACGCAGGCCTGGGCGTGTCCGACCTGcaca                                       >  8:1125/1‑136 (MQ=255)                                                                                                   ggTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACGTCAAGATGTACCAGAACGTTACCGAGATGGCGAACGCAGGCCTGGGCGTGTCCGACCTGCACACCg                                    >  8:169618/1‑136 (MQ=255)                                                                                                   ggTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACGTCAAGATGTACCAGAACGTTACCGAGATGGCGAACGCAGGCCTGGGCGTGTCCGACCTCCACACCg                                    >  4:32955/1‑136 (MQ=255)                                                                                                              cTATGACCTGATCATCCAGGTAGTCTCCGGTGACGTTACCGACCTGAGCAGCTCGAACGTCAAGATGTACAAGAACGTTACCGAGATGGCGAACGCAGGCCTGGGCTTGTCCGACCTGCACACCGTTAATGGTCa                          <  2:190468/135‑1 (MQ=255)                                                                                                                 tGACCTGAACATCCAGGTAGGCTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACGTCAAGATGTACCAGAACGTTAACGAGATGGCGAACGCAGGCATGGGCGTGTCCGACCTGCACACCGTGAATGGTCAGAGc                      >  2:202201/1‑136 (MQ=255)                                                                                                                        aaCATCCAGGTAGGCTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACGTCAAGATGTACCAGAACGTTACCGAGATGGCGAACGCAGGCCTGGGCGTGTCCGACCTGCACACCGTGAATGGTCAGAGCtactac                <  8:106686/135‑1 (MQ=255)                                                                                                                           aTCCAGGTAGGCTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACGTCAAGATGTACCAGAACGTTACCGAGATGGCGAACGCAGGCCTGGGCGTGTCCGACCTGCACACCGTGAATGGTCAGAGCTACTacgacg            >  1:193225/1‑136 (MQ=255)                                                                                                                           aTCCAGGTAGGCTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACGTCAAGATGTACCAGAACGTTACCGAGATGGCGAACGCAGGCCTGGGCGTGTCCGACCTGCACACCGAGAATGGTCAGAGCTACTacgacg            >  6:301942/1‑136 (MQ=255)                                                                                                                           aTCCAGGTAGGCTCCGGTGCCGTTACAGAACTGAGCAGCTCGAACGACAAGATGTACAAGAACGTTACCGAGACGGCGAACGCAGGCCTGGGCGTGTCCGACCTGCACACCGTGAATGGTCAGAGCTACTacgacg            >  3:187651/1‑136 (MQ=255)                                                                                                                           aTCCAGGTAGGCTCCGCTGCCGTTACCGCCCTGAGCAGCTCGAACGTCAAGATGTACCAGAACGTTACCGAGATGGCGAACGCAGGCCTGGGCGAGTCCGACCTGCACACCGTGAATGGTCAGAGCTACTacgacg            >  7:143826/1‑136 (MQ=255)                                                                                                                              cAGGTAGGCTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACGTCAAGATGTACCAGAACGTTACCGAGATGGCGAACGCAGGCCTGGGCGTGTCCGACCTGCACACCGTGAATGGTCAGAGCTACTACGACGGc          <  7:1125/135‑1 (MQ=255)                                                                                                                                ggTAGGCTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACGTCAAGATGTACCAGAACGTTACCGAGATGGCGAACGCAGGCCTGGGCGTGTcc                                                 >  2:387893/1‑94 (MQ=255)                                                                                                                                ggTAGGCTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACGTCAAGATGTACCAGAACGTTACCGAGATGGCGAACGCAGGCCTGGGCGTGTcc                                                 <  1:387893/94‑1 (MQ=255)                                                                                                                                     gCTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACGTCAAGATGTACCAGAACGTTACCGAGATGGCGAACGCAGGCCTGGGCGTGTCCGACCTGCACACCGTGAATGGTCAGAGCTACTACGACGGCTACAAGCt  >  3:237468/1‑136 (MQ=255)                                                                                                                                     |                                                                                                                                        TCACGGCCACCTGGGGAGCCGGTGGCGGGGTGTCGGGTACCTTTACCCCAAATACCAGCGGCTACTACCCGATCGAGGTCTACCATGCCAACCAGTCCGGTCCAGGCAGCTATGACCTGAACATCCAGGTAGGCTCCGGTGCCGTTACCGACCTGAGCAGCTCGAACGTCAAGATGTACCAGAACGTTACCGAGATGGCGAACGCAGGCCTGGGCGTGTCCGACCTGCACACCGTGAATGGTCAGAGCTACTACGACGGCTACAAGCT  >  ACB143/211315‑211582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑