Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 1,466,816 C→A 7.2% A153A (GCG→GCT)  algD ← GDP‑mannose 6‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1431,466,8160CA 7.2% 171.4 / 3.5 57A153A (GCG→GCT) algDGDP‑mannose 6‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (19/33);  new base A (2/2);  total (22/35)
Fisher's exact test for biased strand distribution p-value = 6.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.39e-01

GGGGCGTCGAGCTCTTCGTACAGGGCCTGGAGGATGTCACCGCTGGCGCTGTCCAGTTCGCCGATGACTGTCATCGGTGGCTGGTCGTAGTCCTTGATCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGCCCGAGCAGTCTTCGAGGATCGGGATGACCACGTTCTTGACGGTGCCAGGCAGTACGGTGCTGCGCACCACGATGGTGTGACGGCGGGTGGTGTC  >  ACB143/1466685‑1466941
                                                                                                                                   |                                                                                                                             
ggggCGTCGCGCTCTTCGTACAGGGCCTGGAGGATGTCACCGCTGGCGCTGTCCAGTTCGCCGCTGACTGTCATCGGTGGCTGGTCGTAGTCCTTGATCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACCGCCa                                                                                                                           >  4:116637/1‑136 (MQ=255)
ggggCGTCGAGCTCTTCGTACAGGGCCTGGAGGATGTCACCGCTGGCGCTGTCCAGTTCGCCGCTGACTGTCATCGGTGGCTGGTCGTAGTCCGTGATAGCGGGTCTTTCACGCAGGAATTCCGGGTTGACCGCCa                                                                                                                           >  6:48208/1‑136 (MQ=255)
    cGTCGAGCTCTTCGTACAGGGCCTGGAGGATGTCACCGCTGGCGCCGTCCAGTTCGCCGATGACTGTCATCGGTGGCTGGTCGTAGTCCTTGATCGCGGTGCTTTCACGAAGGAATTACGGGTTGACCGCCACGcc                                                                                                                       >  7:346805/1‑136 (MQ=255)
      tCGAGCTCTTCGTACAGGGCCTGGAGGATGTCACCGCTGGCGCTGTCCAGTTCGCCGATGACTGTCATCGGTGGCTGGTCGTAGTCCTTGATCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACCGCCACGCCGa                                                                                                                     <  3:106645/136‑1 (MQ=255)
         aGCTCTTCGTACAGGGCCTGGAGGATGTCACCGCTGGCGCTGTCCAGTTCGCCGATGACTGTCATCGGTGGCTGGTCGTAGTCCTTGATCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACAGCCACGCCGAAGt                                                                                                                  >  3:288838/1‑136 (MQ=255)
         aGCTCTTCGTACAGGGCCTGGAGGATGTCACCGCTGGCGCTGTCCAGTTCGCCGATGACTGTCATCGGTGGCTGGTCGTAGTCCTTGATCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACAGCCACGCCGAAGt                                                                                                                  >  3:294422/1‑136 (MQ=255)
                     aGGGCCTGGAGGATGTCACCGCTGGCGCTGTCCAGTTCGCCGATGACTGTCATCGGTGGCTGGTCGTAGTCCTTGATCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACAGCCACGCCGAAGTCGACGCCGGCct                                                                                                      <  4:288838/136‑1 (MQ=255)
                     aGGGCCTGGAGGATGTCACCGCTGGCGCTGTCCAGTTCGCCGATGACTGTCATCGGTGGCTGGTCGTAGTCCTTGATCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACAGCCACGCCGAAGTCGACGCCGGCct                                                                                                      <  4:294422/136‑1 (MQ=255)
                           tGGAGGATGTCACCGCTGGCGCTGTCCAGTTCGCCGATGACTGTCATCGGTGGCTGGTCGTAGTCCTTGATCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGc                                                                                                >  2:316591/1‑136 (MQ=255)
                           tGGAGGATGTCACCGCTGGCGCTGTCCAGTTCGCCGATGACTGTCATCGGTGGCTGGTCGTAGTCCTTGATCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGc                                                                                                >  7:176108/1‑136 (MQ=255)
                           tGGAGGATGTCACCGCTGGCGCTGTCCAGTTAGCCGATGACTGTCATCGGTGGGTGGTCGTAGTCCTTGATCGCGGTGCTTTCACGCAGGAATACCGGGTTGACCGCCACGACTAAGTCGACGCCGCCCTTCTTGc                                                                                                >  4:9/1‑136 (MQ=255)
                               ggaTGTCACCGCTGGCGCTGTCCAGTTCGCCGATGACTGTCATCGGTGTCTGGTCGTAGTCCTTGATCGCGGTGCTTTCACGCAGGAATTCCGGGTAGACCGACACGCCGAAGTAGACGCCGGCCTTCTTGCCCgt                                                                                            >  6:257178/1‑135 (MQ=255)
                               ggaTGTCACCGCTGGCGCTGTCCAGTTCGCCGATGACTGTCATCGGTGGCTGGTCGTAGTCCTTGATCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGccc                                                                                              >  5:258485/1‑134 (MQ=255)
                               ggaTGTCACCGCTGGCGCTGTCCAGTTCGCCGATGACTGTCATCGGTGGCTGGTCGTAGTCCTTAATCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGccc                                                                                              >  2:185886/1‑134 (MQ=255)
                                    tCACCGCTGGCGCTGTCCAGTTCGCCGATGACTGTCATCGGTGGCTGGTCGTAGTCCTTGATCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACTGCCACGCCGAAGTCGACGCCGGCCTTCTTGCCCGAGCAGt                                                                                       >  7:322652/1‑136 (MQ=255)
                                     gaCCGCTGGCGCTGTCCAGTTCGCCGATGACTGTCATCGGTGGCTGGTCGTAGTCCTTGATCGCGTTGCTTTCACGCAGGAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGCCCGAGCAGTc                                                                                      <  7:202666/135‑1 (MQ=255)
                                      aCCGCTGGCGCTGTCCAGTTCGCCGATGACTGTCATAGGTGGCTGGTCGAAGTCCTTGATCGCTGTGCTTTCAGGCAGGAATTCAGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGCCCGAGCAGACt                                                                                     >  4:36695/1‑136 (MQ=255)
                                             gcgcTGTCCAGTTCGCCGATGACTGTCATCGGTGGCTGGTCGTAGTCCTTGATCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGCCCGAGCAGTCTTCGAg                                                                                <  3:169126/134‑1 (MQ=255)
                                               gcTGTCCAGTTCGCCGATGACTGTCATCGGTGGCTGGTCGTAGTCCTTGATCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGCCCGAGCAGTCTTCGAGGATc                                                                            >  3:152818/1‑136 (MQ=255)
                                                ctttcgaGTTCGCCGATGACTGTCATTGGTGGCTGGTCGTAGTCCTTGATCGCGGTGCTTTGACGCTGTAATTCCGGGTTGACCGCGACGCGGAAGTCGTGGCCGGCCTTCTTGGCCGAGCAGTCTTCGAGGATCg                                                                           <  8:199320/130‑1 (MQ=255)
                                                cTGTCCAGTTCGCCGATGACTGTCATCGGTGGCTGGTCGTAGTCCTTGATCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGCCCGAGCAGTCTTCGAGGATCg                                                                           >  1:6934/1‑136 (MQ=255)
                                                cTGTCCAGTTCGCCGATGACTGTCATCGGAGGCTGGTCGTAGTCCTTGATCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGCCCGAGCAGTCTTCGATGATCg                                                                           <  7:168010/136‑1 (MQ=255)
                                                     cAGTTCGCCGATGACTGTCATCGGTGGCTGGTCGTAGTCCTTGATCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGCCCGAGCAGTCTTCGAGGATCGGGATg                                                                      <  4:333712/136‑1 (MQ=255)
                                                     cAGTTCGCCGATGACTGTCATCGGTGGCTGGTCGTAGTCCTTGATCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGCCCGAGCAGTCTTCGAGGATCGGGATg                                                                      <  6:112443/136‑1 (MQ=255)
                                                     cAGTTCGCCGATGACTGTCATCGGTGGCTGGTCGTAGTCCTTGATCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGCCCGAGCAGACTTCGAGGATCGGGATg                                                                      <  1:186167/136‑1 (MQ=255)
                                                               aTGACTGTCATCGGTGGCTGGTCGTAGTCCTTGATCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGCCCGAGCAGTCTTCGAGGATCGGGATGACCACGTTCt                                                            <  4:213718/136‑1 (MQ=255)
                                                               aTGACTGTCATCGGTGGCTGGTCGTAGTCCTTGATCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGCCCGAGCAGTCTTCGAGGATCGGGATGACCACGTTCt                                                            <  4:349202/136‑1 (MQ=255)
                                                               aTGACTGTCATCGGTGGCTGGTCGTAGTCCTTGATCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGCCCGAGCAGTCTTCGAGGATCGGGATGACCACGTTCt                                                            <  2:307859/136‑1 (MQ=255)
                                                                tGACTGTCATCGGTGGCTGGTCGTAGTCCTTGATCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGCCCGAGCAGTCTTCGAGGATCGGGATGACCACGTTCtt                                                           >  3:271303/1‑136 (MQ=255)
                                                                      tCATCGGTGGCTGGTCGTAGTCCTTGATCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGCCCGAGCAGTCTTCGAGGATCGGGATGACCACGTTCTTGACg                                                       <  2:561/134‑1 (MQ=255)
                                                                      tCATCGGTGGCTGGTCGTAGTCCTTGATCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGCCCGAGCAGTCTTCGAGGATCGGGATGACCACGTTCTTGACGGt                                                     <  6:46445/136‑1 (MQ=255)
                                                                      gcATCGGTGGCTGGTCGTAGTCCTTGATCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGCCCGAGCAGTCTTCGAGGATCGGGATGACCACGTTCTTGACGGt                                                     <  6:278416/135‑1 (MQ=255)
                                                                         tCGGTGGCTGGTCGTAGTCCTTGATCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGCCCGAGCAGTCTTCGAGGATCGGGATGACCACGTTCTTGACGGTGcc                                                  <  7:191782/136‑1 (MQ=255)
                                                                         tCGGTGGCTGGTCGTAGTCCTTGATCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGCCCGAGCAGTCTTCGAGGATCGGGATGACCACGTTCTTGACGGTGcc                                                  <  6:338911/136‑1 (MQ=255)
                                                                         tCGGTGGCTGGTCGTAGTCCTTGATCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGCCCGAGCAGTCTTCGAGGATCGGGATGACCACGTTCTTGACGGTGcc                                                  <  6:214570/136‑1 (MQ=255)
                                                                         tCGGTGGCTGGTCGTAGTCCTTGATCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGCCCGAGCAGTCTTCGAGGATCGGGATGACCACGTTCTTGACGGTGcc                                                  <  8:53926/136‑1 (MQ=255)
                                                                         tCGGTGGCTGGTCGTAGTCCTTGATCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGCCCGAGCAGTCTTCGAGGATCGGGATGACCACGTTCTTGACGGTGcc                                                  <  1:324763/136‑1 (MQ=255)
                                                                         tCGGTGGCTGGTCGTAGTCCTTGATCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGCCCGAGCAGTCTTCGAGGATCGGGATGACCACGTTCTTGACGGTGcc                                                  <  5:184374/136‑1 (MQ=255)
                                                                         tCGGTGGCTGGTCGTAGTCCTTGATCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGCCCGAGCAGTCTTCGAGGATCGGGATGACCACGTTCTTGACGGTGcc                                                  <  3:249123/136‑1 (MQ=255)
                                                                         gcGGTGGCTGGTCGTAGTCCTTGATCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGCCCGAGCAGTCTTCGAGGATCGGGATGACCACGTTCTTGACGGTGcc                                                  <  2:386907/135‑1 (MQ=255)
                                                                             gggCTGGTCGTAGTCCTTTATAGCGGTGCTTTCACGCAGGAATTCCGGGTTGAACGCCACGCCGAAGTCGACGCCGGCCTTCTTGCCCGAGCAGTCTTCGATGATCTGGATGACCACGTTCTTGACGGTGcc                                                  <  8:323/131‑1 (MQ=255)
                                                                                  ggTCGTAGTCCTTGATCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGCCCGAGCAGTCTTCGAGGATCGGGATGACCACGTTCTTGACGGTGCCAGGCAGTAc                                         >  1:355874/1‑136 (MQ=255)
                                                                                   gTCGTAGTCCTTGATCGCGTTGCTTTCACGCAGGAATTCCGGGTGGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGCCCGAGCAGTCTTCGAGGATCGGGATGACCACGTTCTTGACGGTGCCAGGCAGTACg                                        <  2:44244/136‑1 (MQ=255)
                                                                                      gTAGTCCTTGATCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGCCCGAGCAGTCTTCGAGGATCGGGATGACCACGTTCTTGACGGTGCCAGGCAGTACGGtg                                     <  1:343963/136‑1 (MQ=255)
                                                                                               gATCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGCCCGAGCAGTCTTCGAGGATCGGGATGACCACGTTCTTGACGGTGCCAGGCAGTACGGTGCTGCGca                              <  8:60489/134‑1 (MQ=255)
                                                                                                aTCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGCCCGAGCAGTCTTCGAGGATCGGGATGACCACGTTCTTGACGGTGCCAGGCAGTACGGTGCTGCGcacca                           <  1:403971/136‑1 (MQ=255)
                                                                                                 tCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACCGCCAc                                                                                                                          <  6:249163/40‑1 (MQ=255)
                                                                                                 tCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACCGCCAc                                                                                                                          >  5:249163/1‑40 (MQ=255)
                                                                                                 tCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGccc                                                                                              <  3:131840/68‑1 (MQ=255)
                                                                                                 tCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGccc                                                                                              >  4:131840/1‑68 (MQ=255)
                                                                                                 tCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGCCCGAGCAGTCTTCGAGGATCGGGATGACCACGTTCTTGACGGTGCCAGGCAGTACGGTGCTGCGcaccac                          >  4:232880/1‑136 (MQ=255)
                                                                                                 tCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGCCCGAGCAGTCTTCGAGGATCGGGATGACCACGTTCTTGACGGTGCCAGGCAGTACGGTGCTGCGcaccac                          >  6:258410/1‑136 (MQ=255)
                                                                                                       tGCTTTCACGCAGGAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGCCCGAGCAGTCTTCGAGGATCGGGATGACCACGTTCTTGACGGTGcc                                                  <  5:219244/106‑1 (MQ=255)
                                                                                                       tGCTTTCACGCAGGAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGCCCGAGCAGTCTTCGAGGATCGGGATGACCACGTTCTTGACGGTGcc                                                  >  6:219244/1‑106 (MQ=255)
                                                                                                                    gAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGCCCGAGCAGTCTTCGAGGATCGGGATGACCACGTTCTTGACGGTGCCAGGCAGTACGGTGCTGCGCACCACGATGGTGTGACGGCGggtg       <  1:47847/136‑1 (MQ=255)
                                                                                                                    gAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGCCCGAGCAGTCTTCGAGGATCGGGATGACCACGTTCTTGACGGTGCCAGGCAGTACGGTGCTGCGCACCACGATGGTGTGACGGCGggtg       <  5:243360/136‑1 (MQ=255)
                                                                                                                          cGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGCCCGAGCAGTCTTCGAGGATCGGGATGACCACGTTCTTGACGGTGCCAGGCAGTACGGTGCTGCGCACCACGATGGTGTGACGGCGGGTGGTGTc  <  8:176108/135‑1 (MQ=255)
                                                                                                                                   |                                                                                                                             
GGGGCGTCGAGCTCTTCGTACAGGGCCTGGAGGATGTCACCGCTGGCGCTGTCCAGTTCGCCGATGACTGTCATCGGTGGCTGGTCGTAGTCCTTGATCGCGGTGCTTTCACGCAGGAATTCCGGGTTGACCGCCACGCCGAAGTCGACGCCGGCCTTCTTGCCCGAGCAGTCTTCGAGGATCGGGATGACCACGTTCTTGACGGTGCCAGGCAGTACGGTGCTGCGCACCACGATGGTGTGACGGCGGGTGGTGTC  >  ACB143/1466685‑1466941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑