Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 1,686,855 C→G 27.4% L90V (CTG→GTG)  CJ019_01584 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1431,686,8550CG27.4% 49.1 / 2.4 38L90V (CTG→GTG) CJ019_01584hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (14/13);  new base G (2/9);  total (16/22)
Fisher's exact test for biased strand distribution p-value = 7.76e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCGCCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACGAGTTCCAGCCCTATGGGGTGGACACAGGCGATGCGCCTTATCTGGGCCCGGTGCGC  >  ACB143/1686724‑1686989
                                                                                                                                   |                                                                                                                                      
aGCCGATCGCCCAGCCACGGGCGTGGGGGGGGGGCATTCTCGCCCATCGTGGCCAGCGGGTGCGGGTAATCCACCTGGCCGCCTTTCGCTTTGCCCTGCCGGCGGTCAAACCCACCAGCACCCCCgtggtgctggt                                                                                                                                    <  4:278225/136‑1 (MQ=255)
         cccAGGCACCTGCCTGGGTCGCGGGCATTCTCGCCCATCGTGGCCCGCTGGTTCCGGTCCTCGCCCTTGCCGCCTTGAGCGTTGGCCAGCCGGCGGTGCAACGCACCAGCCCCCGCCTGGGGCGGGGGCACGACCg                                                                                                                           >  6:72383/1‑136 (MQ=255)
         cccAGGCACCTGCCTGGGTCGCGGGCATTCTCGCCCATCGTGGCCAGCTGGTTCCGGTCATCGCCCTTGCCGCCTTGAGCTTTGGCCCGCCGGCGGTGCAACGCACCAGCACCCCCCTGGTGGTGGTGCACTACCg                                                                                                                           >  3:39601/1‑136 (MQ=255)
            aGGCACCTGCCTGGGTCGCGGGCATTCTCGCCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCCGCCGGCGGTGCAACGCACCCGCACCCGCCTGGTGGTGGTGCACTACCGAgg                                                                                                                        >  4:286642/1‑136 (MQ=255)
                       tgGGTCGCGGGCATTCTCGCCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCCCCCGCCTGGTGCTGGTGCACTACCGCGGCGGCCTGCAg                                                                                                              >  3:296911/1‑135 (MQ=255)
                       tgGGTCGCGGGCATTCTCGCCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCGTGAGCTTTGGGCAGCCGGCGGGGCCACGCACCCCCCCCCCCCAGGTGCTGGGGGACGACCCAGGCGGCCGGCCGc                                                                                                             >  3:15294/1‑136 (MQ=255)
                       tgGGTCGCGGGCATTCTAGCCCAACCTGGCCCGCAGGTTCAGGTCATAGACCATGCCGCCATGAGCTTTGGCCAGCCGGCGGTGCAACCCACCAGCACCCCCATTGTGCTTGTGCACTACCGAGGCGGCCTGCCGc                                                                                                             >  6:189725/1‑136 (MQ=255)
                       tgGGTCGCGGGCAGTCTCGCCCATCGTGGCCAGCTGGTTCCGGTCATCGCCCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCACCGCACCAGCCCCCGCCTGGTGCTGGGGCACTACCGCGGCGGCCTGCCGc                                                                                                             >  1:248133/1‑136 (MQ=255)
                          gacgcgGGCATACTCGCCCATCGTGGCCAGGTGGTTCGGGTCAGCGACCTTGCCGACTTGATCTTTGGCCACCCGGCGGTGCAACGACCAACCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTg                                                                                                          <  8:291867/134‑1 (MQ=255)
                           tCGCGGGCATTCTCGCCAATCGTGGCCAGCTGGTTCGGGGCATCGACCTGGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTgg                                                                                                         <  7:93348/136‑1 (MQ=255)
                           gcgcgGGCATTCTCGCCCAGGGGGGCCGGCGGGTTCGGGTCATCGACCTGGCCGCCTTGAGCTTGGCCCAGCCGGCGGGGCAACCAACCAGCACCCGCCGGGGGGTGGTGCACTACCGAGGCGGCCTGCAGCTTgg                                                                                                         <  6:360855/135‑1 (MQ=255)
                                ggaaTTCTCCCCCATGGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCCGCCGGGGGTGCACCGCACCAGCACCCGCCTGGGGCGGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGa                                                                                                    <  6:155765/133‑1 (MQ=255)
                                          cccTTCGGGGCCAGCGGGTTCCGGTCAGCGACCTTGCCGCCTTCCGGTTTGGCCCGCCGGGGGGGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCa                                                                                          <  8:89329/136‑1 (MQ=255)
                                          cccATGGTGCCCAGGTGGTGCCGGTCATCCACCTGCCCGCCTGGCGCTTGGCCCCGCCGGGGGGGCAACCCACCAGCACCCCCGGGGTGGGGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCa                                                                                          <  3:358221/136‑1 (MQ=255)
                                              tCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGCGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGcc                                                                                      <  1:357404/136‑1 (MQ=255)
                                               cGTGGCCCGCGGGTTCCGGGCAGCGACCTTGCCGCCTTGAGCTTTGGCCACCGGGGGGTGCAACCCACCAGCACCCGCCGGGTGGGGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCa                                                                                     <  3:92874/136‑1 (MQ=255)
                                                gTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCAc                                                                                    >  1:317857/1‑136 (MQ=255)
                                                                    aTCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCCCCAGCCCACGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTAGCCAAc                                                                >  8:123219/1‑136 (MQ=255)
                                                                     tCGACCTTGCGGCCTTGCGGTTGGCCCTCCGGGCGGGCCAACGCACCAGCACCCGCGTGGTGCTGGGGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAAcc                                                               <  5:285967/136‑1 (MQ=255)
                                                                     tCGACCTTGCCGCCTTGTGCTTTGGCCAGCCGGCGGTGAAACGCACCAGCACCCGCCTGGTGCGGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAAcc                                                               <  2:212125/136‑1 (MQ=255)
                                                                      ccccgttGCCGCTTTGTGCTTTGGCCCGCCGGGGGTGCAACCCACCCGCACCCGCGGGGTGGTGTGGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCg                                                              <  6:358233/131‑1 (MQ=255)
                                                                             gccgccTTGCGCTTTGCCCCGCCGGCGGTGCACCCCACAAGCCCCCGCCTGGGGGTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACGAg                                                        <  5:62934/135‑1 (MQ=255)
                                                                              cggcgttGCGCTTGGCCCGGCCGGGGGGGCACCGCACCACCCCCGGGGGGGGGCGGTGGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACGAGtt                                                      <  8:12217/131‑1 (MQ=255)
                                                                              ccgccTTGCGCTTGGGCCAGCGGGGGGTGCAACCCACCAGCACCCGCGGGGGGCGGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACGAGtt                                                      <  3:199266/136‑1 (MQ=255)
                                                                              ccgccTTGAGCTTGGGCCCGCCGGGGGGGCACCGCACCCGCACCCGCGGGGGGGTGGGGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACGAGtt                                                      <  5:336411/136‑1 (MQ=255)
                                                                               cgccTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGCTCCTGGAGCAGGCCACCGCAACCCTACGTTGCCAACCGGCCGAGTTc                                                     >  1:182346/1‑136 (MQ=255)
                                                                               cgccTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACGAGTTc                                                     >  1:377476/1‑136 (MQ=255)
                                                                               cgccTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACGAGTTc                                                     >  2:226811/1‑136 (MQ=255)
                                                                               cgccTTGAGCTTTGGCCAGCCGGCGGTGCAACCCACCAGCACCCGACTGGTGCTGGTGCACGACCGACGCGGCCTGCAGCTTGGGCTGATCCTGGAGCAGGCACCCGAAACCCAGCGTTGCCAACCGGACGAGTTc                                                     >  1:160269/1‑136 (MQ=255)
                                                                                   tggcgCTTGGCCCCGCCGGGGGTGAACCGCACCAGCACCCGCGTGGGGGGGGGGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACGAGTTCCAGc                                                 <  8:224931/132‑1 (MQ=255)
                                                                                      aGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCGTGGTGCGGGGGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACGAGTTCCAGccc                                               <  8:111764/135‑1 (MQ=255)
                                                                                        cTTTGGCCCGCCGGCGGTGCAACGCACCAGCACCCGCCGGGGGCGGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACGAGTTCCAGCCCTAt                                            <  2:317857/136‑1 (MQ=255)
                                                                                           gggCCCGCCGGGGGGGCACCGCACCTGCCCCCGCCGGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACGAGTTCCAGCCCTATggg                                         <  2:216649/135‑1 (MQ=255)
                                                                                              ccAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACGAGTTCCAGCCCTATGGGGTg                                      >  7:65227/1‑136 (MQ=255)
                                                                                                     gcggTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACGAGTTCCAGCCCTATGGGGTGGACACAg                               >  5:269304/1‑136 (MQ=255)
                                                                                                      cggCGCAACGCACCAGCACCCGCCGGGGGGGGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACGAGTTCCAGCCCTATGGGGTGGACACAgg                              <  1:316240/136‑1 (MQ=255)
                                                                                                            accccACCAGCACCGCCGGGGGGGTGGTCCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACGAGTTCCAGCCCTATGGGGTGGACACAGGCGATgc                        <  6:8846/132‑1 (MQ=255)
                                                                                                             aCGAACCAGCACCCGCCGGGGGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACGAGTTCCAGCCCTATGGGGTGGACACAGGCGATgcg                       <  5:141888/136‑1 (MQ=255)
                                                                                                                 aCCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCCCCGAACCCCTGCGTTGCCAACCGGACGAGTTCCAGCCCTATGGGGTGGCCACCGGCGATGCGCCtg                   >  6:123727/1‑135 (MQ=255)
                                                                                                                          gggggggtggtggtgcACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACGAGTTCCAGCCCTATGGGGTGGACACAGGCGATGCGCCTTATCTGGGcc          <  8:65227/131‑1 (MQ=255)
                                                                                                                              tggtgctggtgcACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACGAGTTCCAGCCCTATGGGGTGGACACAGGc                             >  5:297470/1‑113 (MQ=255)
                                                                                                                              tggtgctggtgcACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACGAGTTCCAGCCCTATGGGGTGGACACAGGc                             <  6:297470/113‑1 (MQ=255)
                                                                                                                                gtgctgggccACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCACGCCACCGAAACCCTGCGTTGCCAACCGGACGAGTTCCAGCCCAATGGGGTGGACACAGGCGATGCGCCTTATCTGGGCCCGGTg     <  3:92177/135‑1 (MQ=255)
                                                                                                                                   ctggtgcACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAACCCCTGCGTTGCCAACCGGACGAGTTCCAGCCCTATGGGGTGGACACAGGCGATGCGCCTTAGCTGGGCCCGGTgcgc  >  1:366221/1‑135 (MQ=255)
                                                                                                                                   |                                                                                                                                      
AGCCGATCGCCCAGGCACCTGCCTGGGTCGCGGGCATTCTCGCCCATCGTGGCCAGCTGGTTCCGGTCATCGACCTTGCCGCCTTGAGCTTTGGCCAGCCGGCGGTGCAACGCACCAGCACCCGCCTGGTGCTGGTGCACTACCGAGGCGGCCTGCAGCTTGGCCTGATCCTGGAGCAGGCCACCGAAACCCTGCGTTGCCAACCGGACGAGTTCCAGCCCTATGGGGTGGACACAGGCGATGCGCCTTATCTGGGCCCGGTGCGC  >  ACB143/1686724‑1686989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑