Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 1,834,850 T→G 25.9% D57A (GAC→GCC)  ldhA ← D‑lactate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1431,834,8500TG25.9% 65.3 / 9.4 47D57A (GAC→GCC) ldhAD‑lactate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (22/12);  new base G (11/2);  total (33/14)
Fisher's exact test for biased strand distribution p-value = 2.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGAGTAGGCCGGCACCCGCACCACGGCCAGCCCCAAGCGCTGGGCGGCGGCCAGGTCGACGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGACCACCTCAAAGCCATCTGCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCT  >  ACB143/1834715‑1834982
                                                                                                                                       |                                                                                                                                    
ggCGAGTAGGCCGGCACCCGCACCACGGCCAGCCCCAAGCGCTGGGCGGCGGCCAGGTCGACGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGt                                                                                                                                      >  7:322994/1‑136 (MQ=255)
 gCGAGTAGGCCGGCACCCGCACCACGGCCAGCCCCAAGCGCTGGGCGGCGGCCAGGTCGACGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTc                                                                                                                                     <  3:350608/136‑1 (MQ=255)
  cGAGTAGGCCGGCACCCGCACCACGGCCAGCCCCAAGCGCTGGGCGGCGGCCAGGTCGCCGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGtgg                                                                                                                                    >  1:365578/1‑134 (MQ=255)
        ggCCGGCACCCGCACCACGGCCAGCCCCAAGCGCTGGGCGGCGGCCAGGTCGACGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGGGTTCg                                                                                                                              >  1:196018/1‑136 (MQ=255)
          ccGGCACCCGCACCACGGCCAGCCCCAAGCGCTGGGCGGCGGCCAGGTCGACGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGt                                                                                                                             >  7:271154/1‑135 (MQ=255)
            ggCACCCGCACCACGGCCAGCCCCAAGCGCTGGGCGGCGGCCAGGTCGACGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGGCGAGTTCggctt                                                                                                                          >  5:352134/1‑132 (MQ=255)
              cACCCGCACCACGGCCAGCCCCAAGCGCTGGGCGGCGGCCAGGTCGACGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATtg                                                                                                                        <  7:28042/136‑1 (MQ=255)
              cACCCGCACCACGGCCAGCCCCAAGCGCTGGGCGGCGGCCAGGTCGACGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGGGGCGGTGGTCATtg                                                                                                                        >  6:254029/1‑136 (MQ=255)
               aCCCGCACCACGGCCAGCCCCAAGCGCTGGGCGGCGGCCAGGTCGACGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATtga                                                                                                                       <  3:308453/136‑1 (MQ=255)
                 ccGCACCACGGCCAGCCCCAAGCGCTGGGCGGCGGCCAGGTCGACGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCCGCACCGGCGCGGCGAGTTCGGCATtgatg                                                                                                                     >  8:347200/1‑136 (MQ=255)
                 ccGCACCACGGCCAGCCCCAAGCGCTGGGCGGCGGCCAGGTCGACGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATtgat                                                                                                                      >  6:153159/1‑135 (MQ=255)
                   gCACCACGGCCAGCCCCAAGCGCTGGGCGGCGGCCAGGTCGACGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATtgatg                                                                                                                     >  8:159503/1‑134 (MQ=255)
                    caccacGGCCAGCCCCAAGCGCTGGGCGGCGGCCAGGTCGACGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAg                                                                                                                  <  2:191354/136‑1 (MQ=255)
                      ccacGGCCAGCCCCAAGCGCTGGGCGGCGGCCAGGTCGAGGTGGTTGTAGCCGGCCGAGCGCAGGGCGCTCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGGCGAGGTCGGCATTGATGAAGgc                                                                                                                >  8:249202/1‑136 (MQ=255)
                         cGGCCAGCCCCAAGCGCTGGGCGGCGGCCAGGTCGACGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCa                                                                                                             <  5:77878/136‑1 (MQ=255)
                         cGGCCAGCCCCAAGCGCTGGGCGGCGGCCAGGTCGACGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCa                                                                                                             <  4:224792/136‑1 (MQ=255)
                            ccAGCCCCAAGCGCTGGGCGGCGGCCAGGTCGACGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGGCGAGTTCGTCATTGATGAAGgcgccgcc                                                                                                          >  6:331375/1‑132 (MQ=255)
                                  ccAAGCGCTGGGCGGCGGCCAGGTCGACGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGACCACCt                                                                                                     >  1:31264/1‑135 (MQ=255)
                                   cAAGCGCTGGGCGGCGGCCAGGTCGACGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGACCACCTCa                                                                                                   <  8:153299/136‑1 (MQ=255)
                                       cgcTGGGCGGCGGCCAGGTCGACGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGGCGAGTTCGTGATTGATGATGGAGCCGCCCACCTCACAGc                                                                                               >  5:305859/1‑136 (MQ=255)
                                        gcTGGGCGGCGGCCAGGTCGACGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGACCACCTCAAAGc                                                                                               >  7:13344/1‑135 (MQ=255)
                                             gcggcggcCAGGTCGACGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTGGTCATTGATGAAGGCGCAGACCACCGCAAAGCCATcag                                                                                         >  3:372532/1‑134 (MQ=255)
                                             gcggcggcCAGGTCGACGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGTAGGCGCAGACCACCTCAAAGCCATCTg                                                                                         >  1:30690/1‑136 (MQ=255)
                                               ggcggcCAGCTCGACGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCCGCACCGGCGCGTCGAGATCCTCATTGATGAAGGCGCAGACCACCTCCACGCCATCTGcc                                                                                       >  2:248364/1‑136 (MQ=255)
                                                    ccAGGTCGACGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGCCTTCGGGATTGAAGAAGGCGCAGACCACCACAAAGCCATCTGCCAGggc                                                                                  >  5:78152/1‑136 (MQ=255)
                                                    ccAGGTCGACGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGGCGCGTTCGTCCTTGCTGAAGGCGCAGACCACCTCAAAGCCATCTGccccggc                                                                                  >  7:381456/1‑131 (MQ=255)
                                                     cAGGTCGACGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGACCACCTCAAAGCCATCTGCCAGggcg                                                                                 <  8:257691/136‑1 (MQ=255)
                                                     cAGGTCGACGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGACCACCTCAAAGCCATCTGCCAGggcg                                                                                 <  2:395950/136‑1 (MQ=255)
                                                     cAGGTCGACGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGACCACCTCAAAGCCATCTGCCAGggcg                                                                                 <  4:191257/136‑1 (MQ=255)
                                                     cAGGTCGACGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGGCGAGTTCGTCATTGATGAAGGCGCAGACCACCTCAAAGCCATCTGCCAGggcg                                                                                 <  1:141541/136‑1 (MQ=255)
                                                          cGACGTGGGTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGACCACCTCAAAGCCATCTGCCAGGGCGGCGgt                                                                            <  4:359970/136‑1 (MQ=255)
                                                           gACGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGCTCGTCATTGATGAAGGCGCAGACCACCTCAAAGCCATCTGCCAGGGCGGCGgtg                                                                           <  7:194747/136‑1 (MQ=255)
                                                                ggTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTGATTGATGAAGGCGCAGACCACCTCAAAGCCATCTGCCAGGGCGGCGGTGTCGAg                                                                      >  5:129304/1‑136 (MQ=255)
                                                                     tAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGACCACCTCAAAGCCATCTGCCAGGGCGGCGGTGTCGAGGGt                                                                   >  3:401060/1‑134 (MQ=255)
                                                                     tAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGGCGAGGTCGTCATTGATGAAGGCGCAGACCACCTCAAAGCCATCGGCCAGGGCGGCGGTGGCGAGGGt                                                                   >  5:267705/1‑134 (MQ=255)
                                                                          ggccgAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGGCGAGTTCGTCATTGATGAAGGCGCAGCCCACCTCAAAGCCATCTGCCAGGGCGGCGGTGTCGAGGGTCAggcgg                                                            >  3:146059/1‑136 (MQ=255)
                                                                             cgAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTGATTGATTAAGGCGCAGACCACCCCAAAGCCCTCTGCCAGGGCGGCGGGGTGGGGGGTCAcgggggcg                                                         >  4:19864/1‑136 (MQ=255)
                                                                               aGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGCCCACCTCAAAGCCATCTGCCAGGGCGGCGGTGTCGAGGGTCAGGcgggcggg                                                       >  8:284854/1‑136 (MQ=255)
                                                                               aGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGACCACCTCAAAGCCATCTGCCAGGGCGGCGGTGTCGAGGGTCAGGcgggcggg                                                       >  6:199652/1‑136 (MQ=255)
                                                                               aGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGGCGAGTTCGTCATTGATGAAGGCGCAGACCACCGCAAAGCCATCTGCCAGGGCGGCGGTGTAGAGGGTCAGGcggggggg                                                       >  1:97071/1‑136 (MQ=255)
                                                                               aGCGCAGGGAGATCAACAGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTAGATGTAGGCAGTGAGGAAGGCGCCCACCACCCGAAAGCAAGCAGGCAGGGCGGCGGGGGCGAGGGGCCGGctggaggg                                                       >  2:201155/1‑136 (MQ=255)
                                                                                  gcAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTGGTGATTGATGGAGGCGCAGGCCACCGCAAAGCCCTCGGCCAGGGCGGCGGGGTGGAGGGTCAGGCgggcgggcgg                                                    >  4:383141/1‑134 (MQ=255)
                                                                                         gATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGACCACCTCAAAGCCATCTTCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGc                                             >  2:248535/1‑136 (MQ=255)
                                                                                                   gTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGCCCACCTCAAAGCCATCTGCCAGGGCGGCGGTGTCGAGGGGCAGGCGGGCGGGCGGGAAATGCAGCTCCAggg                                   >  3:339859/1‑136 (MQ=255)
                                                                                                     gCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGACCACCTCAAAGCCATCTGCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCg                                 >  4:192277/1‑136 (MQ=255)
                                                                                                     gCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGGGGCGTTGGTCATTGGTGCAGGCGCAGACCCCCACAAAGCCCTCGGCCAGGGCGGCGGGGTGGAGGGGCCGGCGGGCGGGCGGGAAATGCAGCGCCAGGGCg                                 >  5:107680/1‑136 (MQ=255)
                                                                                                                          cagcaCCGGCGCGGCGTGTTCGGCTTTGATGACGGCCCACACCACCACAACGCCATCGGCCAGGGGGGGGGGGTCGAGGGTCTGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTg            <  3:19864/136‑1 (MQ=255)
                                                                                                                             caCCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGACCACCTCAAAGCCATCTGCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCGTGGTGAAg         >  4:81495/1‑136 (MQ=255)
                                                                                                                                     cgTCGAGTTCGTCATTGATGAAGGCGCAGACCACCTCAAAGCCAACTGCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGACCCCCGCAGCCTTGGTGAAGCTTTCCt  >  1:51224/1‑135 (MQ=255)
                                                                                                                                       |                                                                                                                                    
GGCGAGTAGGCCGGCACCCGCACCACGGCCAGCCCCAAGCGCTGGGCGGCGGCCAGGTCGACGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGACCACCTCAAAGCCATCTGCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCT  >  ACB143/1834715‑1834982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑