Predicted mutation
evidence seq id position mutation freq annotation gene description
RA ACB143 1,834,908 T→G 29.7% T38P (ACC→CCC)  ldhA ← D‑lactate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1431,834,9080TG29.7% 41.6 / 9.5 42T38P (ACC→CCC) ldhAD‑lactate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (19/10);  new base G (4/9);  total (23/19)
Fisher's exact test for biased strand distribution p-value = 4.94e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

CGACGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGACCACCTCAAAGCCATCTGCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTCCTTTGCAA  >  ACB143/1834773‑1835037
                                                                                                                                       |                                                                                                                                 
cGACGTGGGTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGACCACCTCAAAGCCATCTGCCAGGGCGGCGgt                                                                                                                                   <  4:359970/136‑1 (MQ=255)
 gACGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGCTCGTCATTGATGAAGGCGCAGACCACCTCAAAGCCATCTGCCAGGGCGGCGgtg                                                                                                                                  <  7:194747/136‑1 (MQ=255)
      ggTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTGATTGATGAAGGCGCAGACCACCTCAAAGCCATCTGCCAGGGCGGCGGTGTCGAg                                                                                                                             >  5:129304/1‑136 (MQ=255)
           tAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGACCACCTCAAAGCCATCTGCCAGGGCGGCGGTGTCGAGGGt                                                                                                                          >  3:401060/1‑134 (MQ=255)
           tAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGGCGAGGTCGTCATTGATGAAGGCGCAGACCACCTCAAAGCCATCGGCCAGGGCGGCGGTGGCGAGGGt                                                                                                                          >  5:267705/1‑134 (MQ=255)
                ggccgAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGGCGAGTTCGTCATTGATGAAGGCGCAGCCCACCTCAAAGCCATCTGCCAGGGCGGCGGTGTCGAGGGTCAggcgg                                                                                                                   >  3:146059/1‑136 (MQ=255)
                   cgAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTGATTGATTAAGGCGCAGACCACCCCAAAGCCCTCTGCCAGGGCGGCGGGGTGGGGGGTCAcgggggcg                                                                                                                >  4:19864/1‑136 (MQ=255)
                     aGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGCCCACCTCAAAGCCATCTGCCAGGGCGGCGGTGTCGAGGGTCAGGcgggcggg                                                                                                              >  8:284854/1‑136 (MQ=255)
                     aGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGACCACCTCAAAGCCATCTGCCAGGGCGGCGGTGTCGAGGGTCAGGcgggcggg                                                                                                              >  6:199652/1‑136 (MQ=255)
                     aGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGGCGAGTTCGTCATTGATGAAGGCGCAGACCACCGCAAAGCCATCTGCCAGGGCGGCGGTGTAGAGGGTCAGGcggggggg                                                                                                              >  1:97071/1‑136 (MQ=255)
                     aGCGCAGGGAGATCAACAGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTAGATGTAGGCAGTGAGGAAGGCGCCCACCACCCGAAAGCAAGCAGGCAGGGCGGCGGGGGCGAGGGGCCGGctggaggg                                                                                                              >  2:201155/1‑136 (MQ=255)
                        gcAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTGGTGATTGATGGAGGCGCAGGCCACCGCAAAGCCCTCGGCCAGGGCGGCGGGGTGGAGGGTCAGGCgggcgggcgg                                                                                                           >  4:383141/1‑134 (MQ=255)
                               gATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGACCACCTCAAAGCCATCTTCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGc                                                                                                    >  2:248535/1‑136 (MQ=255)
                                         gTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGCCCACCTCAAAGCCATCTGCCAGGGCGGCGGTGTCGAGGGGCAGGCGGGCGGGCGGGAAATGCAGCTCCAggg                                                                                          >  3:339859/1‑136 (MQ=255)
                                           gCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGACCACCTCAAAGCCATCTGCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCg                                                                                        >  4:192277/1‑136 (MQ=255)
                                           gCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGGGGCGTTGGTCATTGGTGCAGGCGCAGACCCCCACAAAGCCCTCGGCCAGGGCGGCGGGGTGGAGGGGCCGGCGGGCGGGCGGGAAATGCAGCGCCAGGGCg                                                                                        >  5:107680/1‑136 (MQ=255)
                                                                cagcaCCGGCGCGGCGTGTTCGGCTTTGATGACGGCCCACACCACCACAACGCCATCGGCCAGGGGGGGGGGGTCGAGGGTCTGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTg                                                                   <  3:19864/136‑1 (MQ=255)
                                                                   caCCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGACCACCTCAAAGCCATCTGCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCGTGGTGAAg                                                                >  4:81495/1‑136 (MQ=255)
                                                                           cgTCGAGTTCGTCATTGATGAAGGCGCAGACCACCTCAAAGCCAACTGCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGACCCCCGCAGCCTTGGTGAAGCTTTCCt                                                         >  1:51224/1‑135 (MQ=255)
                                                                                    cGTCATTGATGAAGGCGCAGACCACCTCAACGCCAGCTGCCAGGGCGGCGGTGTGGCGGGTCCGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGt                                                <  7:235749/135‑1 (MQ=255)
                                                                                       cATTGATCACGGCGCTGACCCCCGCAAAGCCATCTGCCTGGGCGGGGGGGTCGTGGGGCCGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTgct                                             <  2:330380/135‑1 (MQ=255)
                                                                                         ttGATGATGGCGCACACCACCTCAAAGCCAGGTCCCCGGGGGGGGTGGTGGTGGGTCTGGGGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTgctgg                                           <  7:296996/135‑1 (MQ=255)
                                                                                           tatgaAGGCGCAGACCACCTCAAAGCAATCTGCCTGGGCGGCGGTGGCGAGGGTCAGGGGGGGGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTgctggct                                         <  7:26762/134‑1 (MQ=255)
                                                                                           gtggacggCGCAGACCCCCGCACAGCCATCTGCCCGGGCGGGGGGGTCGTGGGGCTGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTgctggct                                         <  1:134399/129‑1 (MQ=255)
                                                                                           gaggcaGGCGCATACCACCCACAACCTCTCTGCCGGGGCGGGGTTGTGGCGGTCCGGGGGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTgctggct                                         <  6:61954/130‑1 (MQ=255)
                                                                                                   cgcAGACCACCTCAAAGCCATCTGCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGaacaa                                >  7:98551/1‑136 (MQ=255)
                                                                                                         ccaccTAAACGCCATCTGCCCGGGCGGCGGTGGCGAGGGGCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGgc                          <  2:278881/136‑1 (MQ=255)
                                                                                                           accTCAACGCCATCTGCCCGGGGGGGGGGGGCGCGGGCCAGGCGGGGGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGgcgc                        <  7:284854/136‑1 (MQ=255)
                                                                                                           accTCAAACCCATCTGCCAGGGCGGCGGTGTCGTGGGGCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGgcgc                        <  1:269244/136‑1 (MQ=255)
                                                                                                                 acgCCATGTCCCCGGGCGGGGGGGGCGTGGGGCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATgg                  <  5:27482/134‑1 (MQ=255)
                                                                                                                 accccATCTGCCCGGGCGGCGGGGGCGAGGGTCCGGCGGGGGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATgg                  <  3:273526/133‑1 (MQ=255)
                                                                                                                 aacccAGCTGCCCGGGCGGCGGGGTCGAGGGTCAGGGGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATgg                  <  5:182488/133‑1 (MQ=255)
                                                                                                                 aaGCCATATGCCAGGGCGGCGGTGCGGAGGTTCTGGGGGGCGGCCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATgg                  <  1:314512/136‑1 (MQ=255)
                                                                                                                   gCCATCTGCCTGGGGGGGGGTGTCTAGGGTCCGGCGGGGGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTc                <  7:159503/136‑1 (MQ=255)
                                                                                                                     cATCTGCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCg               >  8:65294/1‑135 (MQ=255)
                                                                                                                     cATCTGCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCg               >  1:209797/1‑135 (MQ=255)
                                                                                                                          gCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTcc         >  6:373741/1‑136 (MQ=255)
                                                                                                                          gCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTcc         >  6:223310/1‑136 (MQ=255)
                                                                                                                          gCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTGTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTcc         >  1:286015/1‑136 (MQ=255)
                                                                                                                           ccAGGGCGGCGGTGGCTTGGGTCCGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTCCt        <  5:123021/136‑1 (MQ=255)
                                                                                                                              gggCGGCTGTGTCTCGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTCCTTTg     <  6:170667/136‑1 (MQ=255)
                                                                                                                                gcggcgGGGGCGTGGGGCCGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTCCttgcaa   <  1:317464/136‑5 (MQ=255)
                                                                                                                                 tggcggTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTCCTTTGCaa  >  8:111062/2‑136 (MQ=255)
                                                                                                                                 cgggggTGTCGTGGGTCAGGGGGGGGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTCCTTTGCaa  <  2:31264/136‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                 
CGACGTGGTTGTAGCCGGCCGAGCGCAGGGCGATCAACCGCGTGCCAGCGGCGGCCAGGCGCTGCAGCACCGGCGCGTCGAGTTCGTCATTGATGAAGGCGCAGACCACCTCAAAGCCATCTGCCAGGGCGGCGGTGTCGAGGGTCAGGCGGGCGGGCTGGAAATGCAGCTCCAGGGCGGTGCCGCCGGCAGCCTTGGTGAAGCTTTCCTGATCGTAGTGCTGGCTGCTGAACAACAGGGCGCGCATGGTCGAACTCCTTTGCAA  >  ACB143/1834773‑1835037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑